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| 1 | Clinical features,diagnosis,treatment and prognosis of multiple primary colorectal carcinoma显示文摘AIM: To investigate the clinical features, diagnosis,treatment and prognosis of multiple primary colorectal carcinomas (MPCC). METHODS: A retrospective analysis of 37 patients with MPCC from 1974 to 1998 was carried out.RESULTS: The incidence of MPCC was 2.74%(37/1 348) in patients with primary colorectal carcinomas, 15 cases of them were patients with synchronous carcinomas (SC) and 22 cases were diagnosed as metachronous carcinomas (MC). Most tumors were located in the right colon and rectum. Fifty-five percent (12/22) of MC were diagnosed within 3 years after tumor resection and 41%(9/22) of MC occurred after 8 years. Radical resections were performed in all patients except for 1 case. The 5-year survival rate of SC was 72.7%(8/11) and that of MC after the first cancer and second cancer was 71.4%(15/21) and 38.9%(7/18), respectively. CONCLUSION: The results indicate the importance of complete preoperative examination, careful intraoperative exploration and periodic postoperative surveillance. Early diagnosis and radical resection can increase survival rate of MPCC. | Hong-ZhiWang Xin-FuHuang Yiwang Jia-FuJi JinGu | 2004 | World Journal of Gastroenterology2004，10，14： | 44 |
| 2 | Activating mechanism of transcriptor NF-kappaB regulated by hepatitis B virus X protein in hepatocellular carcinoma显示文摘AIM:To investigate the mechanism and significance of NF-κB activation regulated by hepatitis B virus X protein (HBx) in hepatitis B virus (HBV)-associated hepatocellular carcinoma (HCC).METHODS:The expression levels of HBx, p65,IκB-α and ubiquitin were detected by immunohistochemistry in HCC tissue microarrays (TMA) respectively, and IκB-α was detected by Western blot in HCC and corresponding liver tissues.RESULTS: The percentage of informative TMA samples was 98.8% in 186 cases with a total of 367 samples. Compared with corresponding liver tissues (60.0%),the HBx expression was obviously decreased in HBV-associated HCC (47.9%,u=2.24,P<0.05).On the contrary, the expressions of p65 (20.6% vs45.3%, u=4.85, P<0.01) and ubiquitin (8.9% vs 59.0%,u=9.68,P<0.01) were notably elevated in HCC.In addition, IκB-α had a tendency to go up. Importantly, positive relativity was observed between HBx and p65 (X^2=10.26,P<0.01), p65 and IκB-α (x^2=16.86,P<0.01), IκB-α and ubiquitin (x^2=8.90, P<0.01) in HCC, respectively.CONCLUSION:Both active and non-active forms of NF-κB are increased in HBV-associated HCC. Variant HBx is the major cause of the enhancement of NF-κB activity. The activation always proceeds in nucleus and the proteasome complexes play an important role in the activation. | TaoWang YiWang Meng-ChaoWu Xin-YuanGuan Zheng-FengYin | 2004 | World Journal of Gastroenterology2004，10，3： | 36 |
| 3 | Effects of ulinastatin on renal ischemia-reperfusion injury in rats显示文摘AIM: To investigate the effect and possible mechanism of ulinastatin on renal ischemia-reperfusion injury in rats. METHODS: Male Sprague-Dawley rats were subjected to 45-min bilateral renal ischemia, treated with intravenously 12 500 U ulinastatin at 30 min prior to ischemia and at the beginning of reperfusion, compared with a nontreated group without ulinastatin and a sham-operation group without bilateral renal ischemia. After 0 h, 2 h, 6 h, 12 h, and 24 h of reperfusion, serum creatinine and blood urea nitrogen were measured for the assessment of renal function, renal sections were used for histologic grading of renal injury, for immunohistochemical localization of Bcl-2 and heat shock protein 70. Renal ultrastructure was observed through a transmission electron microscope. RESULTS: Ulinastatin significantly reduced the increase in blood urea nitrogen and creatinine produced by renal ischemia-reperfusion, suggesting an improvement in renal function. Ulinastatin reduced the histologic evidence of renal damage associated with ischemia-reperfusion and accompanied with an up-regulation in the expression of Bcl-2 protein, but it had no significent effect on the expression of HSP 70. Ulinastatin also significantly reduced kidney ultrastructure damage caused by renal ischemia-reperfusion. CONCLUSION: The protease inhibitor, ulinastatin, reduced the renal dysfunction and injury associated with ischemia-reperfusion of the kidney. The protective effect of ulinastatin might be associated with the up-regulation of Bcl-2 expression and the effect on membrane fragility. | Cong-congCHEN Zi-mingLIU Hui-huaWANG WeiHE YiWANG Wei-dongWU | 2004 | Acta Pharmacologica Sinica2004，25，10： | 20 |
| 4 | Microvessel density of malignant and benign hepatic lesions and MRI evaluation显示文摘AIM. To study the difference of microvessel density (MVD) between malignant and benign hepatic lesions and study the relationship between MVD and dynamic enhanced magnetic resonance imaging (MRI) for evaluation of microvessels within malignant and benign hepatic lesions. METHODS: A total of 265 specimens of hepatocellular carcinoma (HCC), 122 cirrhosis tissues and 22 hepatic benign lesions were enrolled for MVD by immunohistochemistry on tissue microarray, of which 49 underwent MRI examination before surgery, then contrast-to-noise ratios (CNR) and enhancement index (EI) in all the phases were calculated. Pearson correlation was performed for correlation analysis between CNR, EI and MVD. RESULTS: MVD of HCC was 22.7±15.8 (mean±SD), which was obviously higher than that of cirrhosis tissue (8.3±7.6, P<0.01), but was not statistically different from that of benign lesions (31.3±22.7, P>0.05). Among HCC, MVD of gradesⅠ-Ⅱ was 29.9±18.6, which was much higher than those ofgrade Ⅲ (22.2±18.2, P<0.01) and gradeⅣ (22.9±19.0, P<0.01). MVD of HCC (P=0.018) and of benign lesions (P=0.014) were both correlative with CNR in arterial phase. CONCLUSION: Neoangiogenesis is an important feature for malignant tumor, and MVD may act as a biological marker in differentiating malignant from benign hepatic lesions. Dynamic enhanced MRI, especially image in arterial phase, may act as an MVD evaluation criterion for malignant and benign hepatic lesions. | Jian-PingLu JianWang TaoWang YiWang wei-Qingwu LiGao | 2004 | World Journal of Gastroenterology2004，10，12： | 13 |
| 5 | Effect of body mass index on adenocarcinoma of gastric cardia显示文摘AIM: Obesity has been proved as one of the main risk factors for gastric cardia adenocarcinoma (GCA) in the West.The objective of our research was to evaluate the relationship between obesity and the risk of GCA in people from North China.METHODS: A total of 300 patients who had been diagnosed as GCA and had accepted surgical operation at Beijing Cancer Hospital from 1995 to 2002 were enrolled. Data were collected from pathology materials and hospital records. Two hundred and fifty-eight healthy people who had accepted health examination at the same hospital during the same period were enrolled as controls. Height, weight and gender of them at the time of examination were also collected.Obesity was estimated by body mass index (BMI), computed as weight in kilograms per square surface area (Kg/m2).The degree of obesity was determined by using BMI≤ 18.5,24-27.9 and ≥28 (Kg/m2) as the cut-off points for underweight/normal, overweight and obesity, respectively.Associations with obesity were estimated by odds ratios (ORs) and 95 % confidence intervals (CIs). All ORs were adjusted for age and sex.RESULTS: The mean level of BMI was significantly lower in the patient group than that in the control group. The ORs for obesity in age groups 30-59 and 60-79 were 1.15 (95 %CI=0.37-3.65) and 0.16 (95 % CI=0.05-0.44) for males and 0.78 (95 % CI=0.26-2.36) and 0.28 (95 % CI=0.04-2.05)for females, respectively. The ORs for underweight were 2.42 (95 % CI=0.56-10.53) and 4.68 (95 % CI=1.13-19.40)for males in age subgroups 30-59 and 60-79 and 40.7 (95 %CI=9.32-177.92) for females older than 60 yrs. BMI was significantly associated with GCA (P<0.01). Underweight people were at high risk for GCA.CONCLUSION: BMI is an independent risk factor for GCA.Underweight is positively associated with GCA. | JiZhang Xiang-QianSu Xiao-JiangWu Ya-HangLiu HuaWang Xiang-NongZong YiWang Jia-FuJi | 2003 | World Journal of Gastroenterology2003，9，12： | 10 |
| 6 | Method of coupled mode for long-range bottom reverberation显示文摘The theory of coupled mode is used for modeling the long-range bottom reverberation in shallow water caused by bottom roughness. The distant bottom reverberation level and spatial coherence of impulsive source are both derived. The results agree with those from the classical reverberation model, and are compared with the experimental data. The influence of source bandwidth and the distance between sources and receivers on the intensity of bottom reverberation are particularly discussed. The method is shown to be available for both the monoand the bi-static cases. | GAO Bo , YANG ShiE, PIAO ShengChun & HUANG YiWang National Laboratory of Underwater Acoustic Technology,Harbin Engineering University, Harbin 150001, China | 2010 | Science China(Physics,Mechanics & Astronomy)2010，53，12： | 9 |
| 7 | A simple way to make pre-stressed ceramics with high strength显示文摘A pre-stressing design and a simple fabrication technology to substantially improve the strength of ceramic components are presented.Residual surface compressive stress is generated in ceramic components by pressureless sintering of a green bulk coated with a thin layer of low coefficient of thermal expansion(CTE).The stress level can be controlled by changing the cross-section area ratio,Young's modulus ratio and CTE ratio of the coating.Pre-stressed ZrO_(2) ceramics coated with Al_(2)O_(3) can achieve a flexural strength of 1330±52MPa,45% higher than their uncoated counterpart.Similarly,the flexural strength of building porcelain tiles is increased by 70%,from 67±3 MPa to 114±5MPa.The damage tolerance of pre-stressed ZrO_(2) ceramics is excellent with a high residual strength of ~1200 MPa in a thermal shock test at 325℃.This simple technique can improve the mechanical performance of ceramic components with no limitation of size and shape. | Yiwang Bao Fenghua Kuang Yi Sun Yueming Li Detian Wan Zongyang Shen Delong Ma Lingfeng He | 2019 | Journal of Materiomics2019，5，4： | 7 |
| 8 | Establishment of a P-glycoprotein substrate screening model and its preliminary application显示文摘AIM: To establish a high P-glycoprotein (P-gp) expressing cell line as a model for studying drug absorption and distribution, and to explore the preliminary application of this screening model.METHODS: A full-length MDR1 cDNA fragment in plasmid pMDRA1 was first subcloned into plasmid pET28a(+), then MDR1 cDNA was cut from the recombinant plasmid with double-digestion and ligated into the mammalian expression vector pcDNA3.1(+). The recombinant plasmid pcDNA3.1(+)/MDR1 was transfected into breast cancer cell line Bcap37 using the Superfect transfection reagent. Several stably transfected clones were obtained after selection with G418.Real-time fluorescent quantitative RT- PCR and Western blot methods were used to detect the expression of P-gp, and the cellular location of the expressed protein was determined by immunohistochemical staining. Drug sensitivity assay was used to evaluate the biological function of expressed P-gp.Concentration of quercetin in cells was determined by high-performance liquid chromatography (HPLC).RESULTS: The recombinant plasmid was confirmed to be inserted in the correct orientation by restrictive enzyme digestion and DNA sequencing. Real-time fluorescent quantitative RT-PCR showed a higher level of P-gp mRNA in transfected cells compared to that in the control cells, and the Western blot result also indicated that P-gp expression in transfected cells was higher than that in control cells. The immunohistochemical staining showed that the expressed P-gp was localized on cell membranes. Drug sensitivity assay showed that the ICs0 for adriamycin and colchicine of the transfected cells was higher than that of the control cells.The concentration of quercetin in model cells was lower than that in control cells by HPLC. After P-gp inhibitor verapamil was administered, the concentration of quercetin in model cells was increased.CONCLUSION: A high P-gp expressing cell line can be established, which could provide a suitable in vitro model system for studying drug intestinal absorption mechanism,predicting the drug permeability characteristics and screening new multi-drug resistance reversing agents. With this model,quercetin can be found to be transported by P-gp, and it is a P-gp substrate. | YiWang JiangCao SuZeng | 2004 | World Journal of Gastroenterology2004，10，9： | 6 |
| 9 | Recent progress in organic solar cells(PartⅠmaterial science)显示文摘During past several years,the photovoltaic performances of organic solar cells(OSCs)have achieved rapid progress with power conversion efficiencies(PCEs)over 18%,demonstrating a great practical application prospect.The development of material science including conjugated polymer donors,oligomer-like organic molecule donors,fused and nonfused ring acceptors,polymer acceptors,single-component organic solar cells and water/alcohol soluble interface materials are the key research topics in OSC field.Herein,the recent progress of these aspects is systematically summarized.Meanwhile,the current problems and future development are also discussed. | Yahui Liu Bowen Liu Chang-Qi Ma Fei Huang Guitao Feng Hongzheng Chen Jianhui Hou Lingpeng Yan Qingya Wei Qun Luo Qinye Bao Wei Ma Wei Liu Weiwei Li Xiangjian Wan Xiaotian Hu Yanchun Han Yaowen Li Yinhua Zhou Yingping Zou Yiwang Chen Yongfang Li Yongsheng Chen Zheng Tang Zhicheng Hu Zhi-Guo Zhang Zhishan Bo | 2022 | Science China Chemistry2022，65，2： | 5 |
| 10 | Molecular crowding agents engineered to make bioinspired electrolytes for high-voltage aqueous supercapacitors显示文摘The development of low-cost and eco-friendly aqueous electrolytes with a wide voltage window is the key to achieving safe high energy density supercapacitors(SCs).In this work,a molecular crowding electrolyte is prepared by simulating the crowded environment in living cells.Ion transport in the molecular crowding electrolyte can be effectively improved via reducing the molecular weight of the crowding agent,polyethylene glycol(PEG).The results show that PEG with a molecular weight of 200(PEG200)can significantly improve ionic conductivity while maintaining a wide voltage window.These advantages enable commercial activated carbon-based SCs to work at 2.5 V with high energy density,outstanding rate performance and good stability for more than 10,000 cycles.On this basis,three series of molecular crowding electrolytes using sodium perchlorate,lithium perchlorate,and sodium trifluoromethanesulfonate as salts are developed,demonstrating the versatility of PEG200 for wide-voltage aqueous electrolytes. | Mengke Peng Li Wang Longbin Li Zhongyou Peng Xiannong Tang Ting Hu Kai Yuan Yiwang Chen | 2021 | eScience2021，1，1： | 5 |
| 11 | A geometric Model for the Spatial Correlation of an Acoustic Vector Field in Surface-generated Noise显示文摘健全压力和在水平地成层的媒介的表面噪音的粒子速度的空间关联被表明,与一致地在海洋表面上散布的方向性的噪音来源一起。在粒子速度的评估,飞机波浪近似被用于每条事件光线。由于健全来源关联性质和它的择向性的等价,这域的空间关联的解决方案被转变成单身的方向性的来源产生的协调函数的集成。作为一典型水平地成层的媒介,在完美的波导的表面噪音被调查。正常模式和几何模型给的关联系数显示出令人满意的同意。另外,在健全压力之间的规范的协变性和粒子速度的垂直部件与声学的吸收系数成正比,当在半无穷地同类的空间的表面噪音的是零时。 | Yiwang Huang (1) huangyiwang@hrbeu.edu.cn Qunyan Ren (12) Ting Li (3) | 2012 | Journal of Marine Science and Application2012，11，1： | 4 |
| 12 | Geoacoustic inversion based on reflection model of effective density fluid approximation显示文摘In order to obtain the physical and geoacoustic properties of marine sediments,an inverse method using reflection loss of different grazing angles is presented.The reflection loss is calculated according to the reflection model of effective density fluid approximation.A two-step hybrid optimization algorithm combining differential evolution and particle swarm optimization along with Bayesian inversion is employed in estimation of porosity,mean grain size,mass density and bulk modulus of grains.Based on the above physical parameters,geoacoustic parameters,including sound speed and attenuation,are further calculated.According to the numerical simulations,we can draw a conclusion that all the parameters can be well estimated with the exception of bulk modulus of grains.In particular,this indirect inverse method for bottom geoacoustic parameters performs high accuracy and strong robustness.The relative errors are 0.092%and 17%,respectively.Finally,measured reflection loss data of sandy sediments at the bottom of a water tank is analyzed,and the estimation value,uncertainty and correlation of each parameter are presented.The availability of this inverse method is verified through comparison between inverse results and part of measured parameters. | YU Shengqi HUANG Yiwang WU Qiong | 2014 | Chinese Journal of Acoustics2014，33，3： | 4 |
| 13 | Reducing Energy Loss and Morphology Optimization Manipulated by Molecular Geometry Engineering for Hetero-junction Organic Solar Cells显示文摘of main observation and conclusion Molecular geometry engineering is an efective strategy to control the micromorphology and molecularenergy level in organic photovoltaics(OPVs).Two novel copolymers based on alkysilyl-and chloride-functionalied benzodithiophene(BDT)were designed and synthesized for wide bandgap copolymer donor materials in OPVs.It was found that the two copolymers exhited distinctly different proper-ties in active layer when blended with fulerene-fre acceptor T-4.The chloride-functionalited copolymer PBDTCI-TZ2 with deeper molecular energy leveland better coplanar structure induced more ordered aggregation in blend flm.Thus,the device based on PBDTC-TZ exhibits better energy alinmentwith IT-4F and smallr radiative recombination.furthermore,the non-radiative recombination of PBDTCI-TZ.T-4F based device is about 45 mV lowerthan the PBDTS-TZ/T-4F based device,contributing to a lower enery loss(Ein,and a higher open-cicut voltage(Vc).As a resut,the devices based onthe blend of PBDTC-TZ2.IT-4F exhibit a high power conversion efficiency(PCE)of up to 12.2%with a high Vvoe of 0.837 Vv,higher than that of PBDTSi-TZ:IT-4F,of which the PCE is 11.2%with a Voc of 0.781V. | Guodong Xu Huan Rao Xunfan Liao Youdi Zhang Yuming Wang Zhi Xing Ting Hu Licheng Tan Lie Chen Yiwang Chen | 2020 | Chinese Journal of Chemistry2020，38，12： | 4 |
| 14 | Wide Band-gap Two-dimension Conjugated Polymer Donors with Different Amounts of Chlorine Substitution on Alkoxyphenyl Conjugated Side Chains for Non-fullerene Polymer Solar Cells显示文摘In this study,wide bandgap(WBG)two-dimensional(2D)copolymer donors(DZ1,DZ2,and DZ3)based on benzodithiophene(BDT)on alkoxyphenyl conjugated side chains without and with different amounts of chlorine atoms and difluorobenzotriazole(FBTZ)are designed and synthesized successfully for efficient non-fullerene polymer solar cells(PSCs).Three polymer donors DZ1,DZ2,and DZ3 display similar absorption spectra at 300-700 nm range with optional band-gap(Egopt)of 1.84,1.92,and 1.97 eV,respectively.Compared with reported DZ1 without chlorine substitution,it is found that introducing chlorine atoms into the meta-position of the alkoxyphenyl group affords polymer possessing a deeper the highest occupied molecular orbital(HOMO)energy level,which can increase open circuit voltage(Voc)of PSCs,as well as improve hole mobility.Non-fullerene bulk heterojunction PSCs based on DZ2:MelC demonstrate a relatively high power conversion efficiency(PCE)of 10.22%with a Voc of 0.88 V,a short-circuit current density(Jsc)of 17.62 mA/cm^2,and a fill factor(FF)of 68%,compared with PSCs based on DZ1:MelC(a PCE of 8.26%)and DZ3:MelC(a PCE of 6.28%).The results imply that adjusting chlorine atom amount on alkoxyphenyl side chains based on BDT polymer donors is a promising approach of synthesizing electron-rich building block for high performance of PSCs. | Youdi Zhang Yong Wang Ruijie Ma Zhenghui Luo Tao Liu So-Huei Kang He Yan Zhongyi Yuan Changduk Yang Yiwang Chen | 2020 | Chinese Journal of Polymer Science2020，38，8： | 3 |
| 15 | Mechanical and thermal properties of polypeptide modified hydroxyapatite/poly(L-lactide) nanocomposites显示文摘A new type of polypeptide(poly(-benzyl-L-glutamate)(PBLG))modified hydroxyapatite(HA)/poly(L-lactide)(PLLA)nanocomposites(PBLG-g-HA/PLLA)were prepared by the solvent-mixing method,and their mechanical and thermal properties were investigated.The tensile test showed that the mechanical properties of PBLG-g-HA/PLLA nanocomposites were better than that of PLLA,even a 0.3 wt%content of PBLG-g-HA in the nanocomposites could make the tensile strength 12%higher than that of the neat PLLA sample,and the tensile modulus was about 17%higher than that of the PLLA sample.The thermal gravimetric analysis(TGA)showed that the PBLG-g-HA/PLLA composites have better thermal stability than the PLLA sample.The differential scanning calorimetry(DSC)was used to characterize the effect of PBLG-g-HA on the crystallization of PLLA.The isothermal crystallization behavior showed that the half crystallization time(t1/2)of PBLG-g-HA/PLLA was much shorter than that of the PLLA sample.When the PBLG-g-HA content was 10%,t1/2 was only 18.7 min,while t1/2 of the PLLA sample was 61.4 min.The results showed that the PBLG-g-HA worked as a nucleating agent and enhanced the crystallization speed of PLLA. | WEI JunChao DAI YanFeng CHEN YiWang CHEN XueSi | 2011 | Science China Chemistry2011，54，3： | 3 |
| 16 | Recent progress in organic solar cells(PartⅡdevice engineering)显示文摘1 Introduction Organic solar cells(OSCs)belong to a multidisciplinary field composed of chemistry,materials science,physics,engineering,etc.For a better reviewing of this field,we briefly divide the research field of OSCs into two parts:material science and device engineering.In our previous review,the material science part of OSCs,including conjugated polymer donors and acceptors,small molecular donors and acceptors. | Yahui Liu Bowen Liu Chang-Qi Ma Fei Huang Guitao Feng Hongzheng Chen Jianhui Hou Lingpeng Yan Qingya Wei Qun Luo Qinye Bao Wei Ma Wei Liu Weiwei Li Xiangjian Wan Xiaotian Hu Yanchun Han Yaowen Li Yinhua Zhou Yingping Zou Yiwang Chen Yuqiang Liu Lei Meng Yongfang Li Yongsheng Chen Zheng Tang Zhicheng Hu Zhi-Guo Zhang Zhishan Bo | 2022 | Science China Chemistry2022，65，8： | 3 |
| 17 | Additive-free non-fullerene organic solar cells with random copolymers as donors over 9% power conversion efficiency显示文摘In this study, a series of random conjugated polymers (PBDB-TBTn) as donors were designed and synthesized. In these polymers, benzodithiophene unit with thiophene conjugated side chains (BDT) are donor part, and two different content of benzo[1,2-c:4,5-c']dithiophene-4,8-dione (BDD) and difluorobenzothiadizole (BT) linked alkylthiophene are acceptor unit. Polymer solar cells (PSCs) were fabricated with ITIC as an acceptor, and over the power conversion efficiency (PCE) of 9%was obtained, with open circuit voltage (Voc) of 0.86 V, short-circuit current density (Jsc) of 16.84 mA/cm^2, and fill factor (FF) of 62.5%. These random conjugated polymers based solar cells are insensitive to solvent additives and thermal annealing. The performance of the device decreases gradually with the increasing of the proportion of fluorinated acceptor unit. The declining efficiency is due to the excessive fluorinated acceptor unit, which leads to over aggregated topography, destroys the effective charge transport pathways, and affects phase separation domain size between the donor and the acceptor. The phenomena are explained by the charge carrier recombination, atomic force microscope (AFM), and transmission electron microscope (TEM). These results indicate that proper addition of fluorinated acceptor units to build random copolymers can enhance the efficiency of organic photovoltaics toward additive-free and thermal annealing-free PSCs. | Meimei Wu Laitao Shi Yu Hu Lie Chen Ting Hu Youdi Zhang Zhongyi Yuan Yiwang Chen | 2019 | Chinese Chemical Letters2019，30，6： | 3 |
| 18 | A key‘foxy’aroma gene is regulated by homologyinduced promoter indels in the iconic juice grape‘Concord’显示文摘‘Concord’,the most well-known juice grape with a parentage of the North American grape species Vitis labrusca L.,possesses a special‘foxy’aroma predominantly resulted from the accumulation of methyl anthranilate(MA)in berries.This aroma,however,is often perceived as an undesirable attribute by wine consumers and rarely noticeable in the common table and wine grape species V.vinifera.Here we discovered homology-induced promoter indels as a major genetic mechanism for species-specific regulation of a key‘foxy’aroma gene,anthraniloyl-CoA:methanol acyltransferase(AMAT),that is responsible for MA biosynthesis.We found the absence of a 426-bp and/or a 42-bp sequence in AMAT promoters highly associated with high levels of AMAT expression and MA accumulation in‘Concord’and other V.labrusca-derived grapes.These promoter variants,all with direct and inverted repeats,were further confirmed in more than 1,300 Vitis germplasm.Moreover,functional impact of these indels was validated in transgenic Arabidopsis.Superimposed on the promoter regulation,large structural changes including exonic insertion of a retrotransposon were present at the AMAT locus in some V.vinifera grapes.Elucidation of the AMAT genetic regulation advances our understanding of the‘foxy’aroma trait and makes it genetically trackable and amenable in grapevine breeding. | Yingzhen Yang JoséCuenca Nian Wang Zhenchang Liang Honghe Sun Benjamin Gutierrez Xiaojun Xi Jie Arro YiWang Peige Fan Jason Londo Peter Cousins Shaohua Li Zhangjun Fei Gan-Yuan Zhong | 2020 | Horticulture Research2020，7，1： | 3 |
| 19 | Effects of adrenomedullin gene overexpression on biological behavior of hepatic stellate cells显示文摘AIM: To investigate the effects of adrenomedullin (AM) gene overexpression on the biological characteristics of human hepatic stellate cells (hHSCs) by stable transfection.METHODS: hHSCs which express low basal levels of AM were stably transfected with an expression construct containing rat AM gene or with an empty expression vector. Expression of AM in hHSCs was determined by reverse transcription (RT)-polymerase chain reaction (PCR) and radioimmunoassay (RIA). Cell proliferation was evaluated by 5-bromo-2'-deoxyuridine (BrdU) incorporation and immunocytochemistry. RT-PCR and Western blot were used to test the expression of procollagen types Ⅰ and Ⅲ. Protein expressions of interstitial collagenase (MMP-1), gelatinase (MMP-2) and tissue inhibitors of matrix metalloproteinases-2 (TIMP-2) were assessed by Western blot.RESULTS: Two cell clones (A-2, A-8) transfected withthe AM gene expressed higher levels of AM mRNA (nontransfected group: 0.86±0.11, empty vector group: 1.01±0.11, A-2 clone group: 1.44±0.08 and A-8 clone group: 1.36±0.05) and protein (12.31±0.17, 12.35±0.12,12.56±0.06 and 12.62±0.07) (P<0.05). AM geneoverexpression had inhibitory effects on cell proliferation of hHSCs (29.6%, 30.9%, 18.9% and 21.8%, respectively. P<0.05) and expression of procollagen type Ⅰ (0.58±0.1,0.48±0.11, 0.3±0.06 and 0.31±0.07 at mRNA level)(0.27±0.07, 0.3±0.06, 0.14±0.05 and 0.13±0.05 at protein level) (P<0.05) and procollagen type Ⅲ (0.17±0.04, 0.15±0.03, 0.1±0.02 and 0.09±0.02 at mRNA level) (0.22±0.04, 0.2±0.03, 0.11±0.04 and 0.13±0.03 at protein level) (P<0.05). Compared with cells non-transfected (TIMp2: 2.77±0.03, MMP-2: 0.5±0.04, MMP-1: 0.49±0.07) and transfected with empty vector (TIMP2: 2.79±0.04,MMP-2: 0.48±0.03, MMP-1: 0.45±0.09), these two clones had lower expression levels of TIMP2(A-2 clone group: 2.7±0.02 and A-8 clone group: 2.71±0.02) (P<0.05) and MMP-2(A-2 clone group: 0.15±0.05 and A-8 clone group: 0.13±0.04) (P<0.05) but displayed a higher expression level of MMP-1(A-2 clone group: 0.68±0.06 and A-8 clone group: 0.81±0.09) (P<0.05).CONCLUSION: AM gene exerts negative influence to some extent on hHSCs by inhibiting proliferation and production of extracellular matrix (ECM) in addition to inducing MMP-1 expression. | YiWang Jin-ShengZhang Guang-CunHuang QiCheng Zhong-HuaZhao | 2005 | World Journal of Gastroenterology2005，11，23： | 3 |
| 20 | Large-area Flexible Organic Solar Cells: Printing Technologies and Modular Design显示文摘Flexibility is the most prominent advantage of organic solar cells(OSCs) compared with traditional photovoltaic devices, showing an irreplaceable commercial potential. Currently, the maximum power conversion efficiencies(PCEs) of single-junction OSCs have been over 19% and 16% upon rigid and flexible substrates, respectively, which meet the criteria for commercial application. Extensive research efforts are under way, such as device configuration design, interface/photosensitive layer synthesis, transparent electrode modification and printing technology innovation, however, the reasonable selection of printing technologies, the huge performance loss of large-area printing process and the structural design of flexible modules are still the bottlenecks, limiting the commercialization of OSCs. This review focuses on the technical challenges and rational modular configuration design for printing preparation of flexible high-efficiency large-area organic devices, from the aspects of the functional layer material selection, printing process research status and large-scale efficiency losses. These will promote the integrated applications of printable organic semiconductor materials for next-generation clean energy and appeal extensive attentions in wearable electronics, building-integrated photovoltaics and Internet of Things, etc. | Xiangchuan Meng Zhi Xing Xiaotian Hu Yiwang Chen | 2022 | Chinese Journal of Polymer Science2022，40，12： | 2 |
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