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19篇 您的检索式:作者名="Yikun He"
    题名 作者 年代 出处 被引量
1CDP1, a novel component of chloroplast division site positioning system in Arabidopsis显示文摘叶绿体是从 endosymbiotic cyanobacteria 演变的植物特定的细胞器。他们通过二进制分裂划分。叶绿体部门地点的选择为对称的叶绿体部门是枢轴的。在 E。coli,在房间的中点部门地点放被 Min 系统的动态摆动调整,它包括 MinC,头脑和矿。在植物的头脑和矿的相当或相同的事物涉及叶绿体分割。MinC 的相当或相同的事物仍然没在更高的植物被识别。然而,象 FtsZ 一样蛋白质, ARC3,被发现地点放涉及叶绿体分割。这里,我们报导放 1 的那个叶绿体部门地点(AtCDP1 ) 是在 Arabidopsis 涉及叶绿体部门地点放置的新奇叶绿体部门蛋白质。AtCDP1 被与房间部门显型为殖民地在细菌屏蔽一个 Arabidopsis cDNA 表示图书馆发现。AtCDP1 只在 Arabidopsis 在年轻绿纸巾被表示。有多重部门地点的伸长的叶绿体在 loss-of-function cdp1 异种被观察。AtCDP1 的 Overexpression 也引起了一个叶绿体部门显型。蛋白质相互作用试金建议 AtCDP1 可以调停通过和 ARC3 的相互作用放的叶绿体部门地点。总的来说,我们的结果显示 AtCDP1 是放系统,和这个系统的工作机制的叶绿体部门地点的一个新奇部件与在原核生物的房间的传统的 MinCDE 系统的不同。Min Zhang Yong Hu Jingjing Jia Dapeng Li Runjie Zhang Hongbo Gao Yikun He 2009Cell Research2009,19,7:5
2A transceptor-channel complex couples nitrate sensing to calcium signaling in Ambidopsis显示文摘Nitrate-induced Ca^(2+) signaling is crucial for the primary nitrate response in plants.However,the molecular mechanism underlying the generation of the nitrate-specific calcium signature remains unknown.We report here that a cyclic nucleotide-gated channel(CNGC)protein,CNGC15,and the nitrate transceptor(NRT1.1)constitute a molecular switch that controls calcium influx depending on nitrate levels.The expression of CNGC15 is induced by nitrate,and its protein is localized at the plasma membrane after establishment of young seedlings.We found that disruption of CNGC15 results in the loss of the nitrate-induced Ca^(2+) signature(primary nitrate response)and retards root growth,reminiscent of the phenotype observed in the nrt1.1 mutant.We further showed that CNGC15 is an active Ca^(2+)-permeable channel that physically interacts with the NRT1.1 protein in the plasma membrane.Importantly,we discovered that CNGC15-NRT1.1 interaction silences the channel activity of the heterocomplex,which dissociates upon a rise in nitrate levels,leading to reactivation of the CNGC15 channel.The dynamic interactions between CNGC15 and NRT1.1 therefore control the channel activity and Ca^(2+) influx in a nitrate-dependent manner.Our study reveals a new nutrient-sensing mechanism that utilizes a nutrient transceptor-channel complex assembly to couple nutrient status to a specific Ca^(2+) signature.Xiaohan Wang Changxin Feng LiLi Tian Congcong Hou Wang Tian Bin Hu Qian Zhang Zhijie Ren Qi Niu Jiali Song Dongdong Kong Liangyu Liu Yikun He Ligeng Ma Chengcai Chu Sheng Luan Legong Li 2021Molecular Plant2021,14,5:5
3Cloning of plastid division gene GlFtsZ from Gentiana lutea and its expression during petal development显示文摘A full-length cDNA of GlFtsZ was isolated by screening the cDNA library of Gentiana lutea. Analysis of the deduced amino acid sequence encoded by GlFtsZ indicated that GlFtsZ protein possesses the typical conservative motifs existed in all FtsZ proteins. The existence of putative plastid transit peptide in its N-terminus suggested that GlFtsZ might function inside of plastids. With the deve- lopmental process of petals of Gentiana lutea, the expression of plastid division gene GlFtsZ declined gradually, whereas the expression of carotenoids biosynthesis gene Zds increased obviously; meanwhile, in contrast to the increment of carotenoids, the content of chlorophyll in petals decreased sharply. The chloroplasts turned into chromoplasts, and the color of petals also turned from green to golden. All of these results suggested that the expression of GlFtsZ is accompanied with the development and differentiation of plastids.HE Yikun , ZHU Changfu , WANG Dong, KONG Dongdong and SUN Jingsan(1. Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China 2. Department of Biology, Capital Normal University, Beijing 100037, China 3. College of Life Science, Northeast Normal University, Changchun 130024, China) 2002Progress in Natural Science:Materials International2002,12,8:5
4Cloning of two plastid division ftsZ genes from Nicotiana tabacum and their expression in E. coli显示文摘Two cDNAs of plastid division gene NtFtsZ1-1 and NtFtsZ1-2 are isolated from Nicotiana tabacum by RT-PCR and rapid amplification cDNA ends (RACE) method. Analysis of the deduced amino acid sequences encoded by NtFtsZ1-1 and NtFtsZ1-2 indicate that these two proteins possess the typical conservative motifs and GTP binding sites existing in all FtsZ proteins. The existence of putative plastid transit peptide in their N-terminal suggests that there are at least two transit-peptide containing FtsZ proteins in higher plants. Phylogenetic analysis based on amino acid sequences of FtsZ proteins also supports this interference. These two NtFtsZ genes demonstrate a similar expression pattern during the plant development, detected by Northern blot. Expression of NtFtsZ1-1 and NtFtsZ1-2 in E.coli interrupts the normal division process of host cells. These results suggest the diverse functions of FtsZ proteins in higher plants.WANG Dong KONG Dongdong HU Yong JU Chuanli HE Yikun and SUN Jingsan(1. Department of Biology, Capital Normal University, Beijing 100037, China 2. Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China 3. College of Life Sciences, Beijing Normal University, Beijing 100875, China) 2002Progress in Natural Science:Materials International2002,12,12:4
5PIF3 Is Involved in the Primary Root Growth Inhibition of Arabidopsis Induced by Nitric Oxide in the Light显示文摘PHYTOCHROME 交往 FACTOR3 (PIF3 ) 是在表明小径的 phytochrome 的一个重要部件并且调停植物 responsesto 各种各样的环境条件。我们发现 PIF3 涉及氮的氧化物导致的 Arabidopsis thaliana 幼苗的根生长的抑制(没有) 在光。PIF3 的 Overexpression 部分减轻了禁止的效果不在根生长上,而 pif3-1 异种显示了提高的敏感到不以根生长。在 phytochrome 发信号期间,光敏电阻器 PHYBmediates PIF3 的降级。我们发现 phyB-9 异种而且以应答有类似的显型到 PIF3ox 的到号码,没有处理支持了 PHYB 的累积,并且因此进一步减少了 PIF3 content.Our 结果证明 PIF3 的活动被 DELLA 蛋白质 RGL3 调整[象 3 一样的 RGA ( ga1-3 的抑压者)]。因此,我们推测那 PIF3 PHYB 和 RGL3 下游地躺,并且在禁止的效果起一个重要作用不在在 light.Key 词的 Arabidopsis 幼苗的根生长上:Sulan Bai Tao Yao Miaomiao Li Xiaomin Guo Yaochuan Zhang Shengwei Zhu Yikun He 2014Molecular Plant2014,7,4:3
6A chlorinated polymer promoted analogue co-donors for efficient ternary all-polymer solar cells显示文摘The efficient ternary all-polymer solar cells(PSCs) are designed and fabricated, using a polymer acceptor of NDP-V-C7 and analogue co-donors containing a chlorinated polymer PBCl T and classical PTB7-Th. PBCl T and PTB7-Th possess very similar chemical structure and matched energy levels to form the cascade of the co-donors. Meanwhile, benefiting from those analogous polymer structures, there is little influence of the morphology in blend film compared to their pristine polymer films. The binary PBCl T:NDP-V-C7 devices exhibit a high open-circuit voltage(V_(oc)) due to the deep HOMO level of PBCl T. The V_(oc)of all-PSCs could be finely manipulated by adjusting the content of PBCl T in blend film. The ternary all-PSCs have the more balanced charge mobility and prolonged carrier lifetime compared to the binary devices. The PBCl T also help improve the miscibility of ternary blend and suppress crystallization in films, bringing about favorable morphology with appropriate orientation and surface roughness in blend film. With the optimal processing, the champion ternary all-PSCs obtain a high PCE of 9.03%, which is about 10% enhancement compared to that of binary device. The results indicate that the ternary approach using analogue co-donors is a practical method to enhance the performance of all-PSCs.Hui Chen Yikun Guo Pengjie Chao Longzhu Liu Wei Chen Dahui Zhao Feng He 2019Science China Chemistry2019,62,2:3
7Targeted deletion of mouse Rad1 leads to deficient cellular DNA damage responses显示文摘The Rad1 gene is evolutionarily conserved from yeast to human.The fission yeast Schizosaccharomyces pombe Rad1 ortholog promotes cell survival against DNA damage and is required for G_(2)/M checkpoint activation.In this study,mouse embryonic stem(ES)cells with a targeted deletion of Mrad1,the mouse ortholog of this gene,were created to evaluate its function in mammalian cells.Mrad1^(−/−)ES cells were highly sensitive to ultraviolet-light(UV light),hydroxyurea(HU)and gamma rays,and were defective in G_(2)/M as well as S/M checkpoints.These data indicate that Mrad1 is required for repairing DNA lesions induced by UV-light,HU and gamma rays,and for mediating G_(2)/M and S/M checkpoint controls.We further demonstrated that Mrad1 plays an important role in homologous recombination repair(HRR)in ES cells,but a minor HRR role in differentiated mouse cells.Chunbo Zhang Yuheng Liu Zhishang Hu Lili An Yikun He Haiying Hang 2011Protein & Cell2011,2,5:3
8Ultra-small hollow ternary alloy nanoparticles for efficient hydrogen evolution reaction显示文摘Hollow nanoparticles with large specific surface area and high atom utilization are promising catalysts for the hydrogen evolution reaction(HER). We describe herein the design and synthesis of a series of ultra-small hollow ternary alloy nanostructures using a simple one-pot strategy. The same technique was demonstrated for hollow PtNiCu nanoparticles, hollow PtCoCu nanoparticles and hollow CuNiCo nanoparticles. During synthesis, the displacement reaction and oxidative etching played important roles in the formation of hollow structures. Moreover, our hollow PtNiCu and PtCoCu nanoparticles were single crystalline, with an average diameter of 5 nm. Impressively, ultra-small hollow PtNiCu nanoparticles,containing only 10% Pt, exhibited greater electrocatalytic HER activity and stability than a commercial Pt/C catalyst. The overpotential of hollow Pt Ni Cu nanoparticles at 10 mA cm^(-2) was 28 mV versus reversible hydrogen electrode(RHE). The mass activity was 4.54 A mg Pt^(-1) at-70 mV versus RHE, which is 5.62-fold greater than that of a commercial Pt/C system(0.81 A mg Pt^(-1)). Through analyses of bonding and antibonding orbital filling, density functional theory calculations demonstrated that the bonding strength of different metals to the hydrogen intermediate(H*) was in the order of Pt > Co > Ni > Cu. The excellent HER performance of our hollow PtNiCu nanoparticles derives from moderately synergistic interactions between the three metals and H*. This work demonstrates a new strategy for the design of low-cost and high-activity HER catalysts.Zhenxing Li Chengcheng Yu Yikun Kang Xin Zhang Yangyang Wen Zhao-Kui Wang Chang Ma Cong Wang Kaiwen Wang Xianlin Qu Miao He Ya-Wen Zhang Weiyu Song 2021National Science Review2021,8,7:2
9Target deletion of the AAA ATPase PpCDC48II in Physcomitrella patens results in freezing sensitivity after cold acclimation显示文摘CDC48 is a highly conserved protein in eukaryotes and belongs to the AAA (ATPase associated with a variety of cellular activities) superfamily. It can interact with many different co-factors and form protein complexes that play important roles in various cellular processes. According to the Physcomitrella patens database, one member of the ATPases, the cell cycle gene PpCDC48II, was cloned. PpCDC48II contains two typical ATPase modules and is highly homologous to AtCDC48A. PpCDC48II was up-regulated in mRNA levels after incubation at 0°C for 36 and 72 h. To further elucidate protein function, we disrupted the PpCDC48II gene by transforming P. patens with the corresponding linear genomic sequences. When treated to the same freezing stress, it was found that PpCDC48II knockout plants were less resistant to freezing treatment than wild type after acclimation. This suggested that PpCDC48II was an essential gene for low-temperature-induced freezing tolerance in P. patens cells.WANG Hui JIN Shuo CHEN Xi GEN XuKe HE YiKun 2012Science China(Life Sciences)2012,55,2:1
10A chloride efflux transporter,BIG RICE GRAIN 1,is involved in mediating grain size and salt tolerance in rice显示文摘Grain size is determined by the size and number of cells in the grain.The regulation of grain size is crucial for improving crop yield;however,the genes and molecular mechanisms that control grain size remain elusive.Here,we report that a member of the detoxification efflux carrier/Multidrug and Toxic Compound Extrusion(DTX/MATE)family transporters,BIG RICE GRAIN 1(BIRG1),negatively influences grain size in rice(Oryza sativa L.).BIRG1 is highly expressed in reproductive organs and roots.In birg1 grain,the outer parenchyma layer cells of spikelet hulls are larger than in wild-type(WT)grains,but the cell number is unaltered.When expressed in Xenopus laevis oocytes,BIRG1 exhibits chloride efflux activity.Consistent with this role of BIRG1,the birg1 mutant shows reduced tolerance to salt stress at a toxic chloride level.Moreover,grains from birg1 plants contain a higher level of chloride than those of WT plants when grown under normal paddy field conditions,and the roots of birg1 accumulate more chloride than those of WT under saline conditions.Collectively,the data suggest that BIRG1 in rice functions as a chloride efflux transporter that is involved in mediating grain size and salt tolerance by controlling chloride homeostasis.Zhijie Ren Fenglin Bai Jingwen Xu Li Wang Xiaohan Wang Qian Zhang Changxin Feng Qi Niu Liying Zhang Jiali Song Fang Bao Liangyu Liu Yikun He Ligeng Ma Wang Tian Congcong Hou Legong Li 2021Journal of Integrative Plant Biology2021,63,12:1
11Nitric oxide suppresses stomatal opening by inhibiting inward-rectifying K_(in)^+ channels in Arabidopsis guard cells显示文摘We explore nitric oxide (NO) effect on K+in channels in Arabidopsis guard cells. We observed NO inhib- ited K+in currents when Ca2+ chelator EGTA (Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid) was not added in the pipette solution; K+in currents were not sensitive to NO when cytosolic Ca2+ was chelated by EGTA. NO inhibited the Arabidopsis stomatal opening, but when EGTA was added in the bath solution, inhibition effect of NO on stomatal opening vanished. Thus, it implies that NO ele- vates cytosolic Ca2+ by activating plasma membrane Ca2+ channels firstly, then inactivates K+in chan- nels, resulting in stomatal opening suppressed subsequently.XUE ShaoWu YANG Pin HE YiKun 2008Chinese Science Bulletin2008,53,14:1
12ftsZ gene and plastid division显示文摘Plastid is one of the most important cellularorganelles, the normal division process of plastid is essential for the differentiation and development of plant cells. For a long time, morphological observations and genetic analyses to special mutants are the major research fields of plastiddivision, but the molecular mechanisms underlying plastiddivision are largely unknown. Because of the endosymbiotic origin, plastid division might have mechanisms in common with those involved in bacterial cell division. It has beenproved that several prokaryotic cell division genes also par-ticipate in the plastid division. Recently, the mechanisms of prokaryotic cell division have been well documented, which provides a valuable paradigm for understanding the plastid division mechanisms. In plants, the functional analyses of ftsZ, a key gene involved both in bacteria and plastid division, have established the solid foundation for people to under-stand the plastid division in molecular level. In this paper we will make a review for the research history and progress of plastid division.KONG Dongdong WANG Dong HU Yong JU Chuanli WANG Yingdian HE Yikun SUN Jingsan 2003Chinese Science Bulletin2003,48,12:1
13α-Ketoglutarate dehydrogenase(KGDH):A new balancer between energy metabolism and gene expression in plants显示文摘Sessile plants can adjust their growth and development in response to environmental stimuli.Sunlight(light),is the primary source of energy for all life on the earth,in the long course of phylogenetic evolution,plants have developed a unique relationship with light:they can absorb light energy and convert it into organic matter for use by themselves and others。Wenwen Zhu Yikun He 2023Journal of Integrative Plant Biology2023,65,8:0
14Exploiting the point spread function for optical imaging through a scattering medium based on deconvolution method显示文摘Visual perception of humans penetrating turbid medium is hampered by scattering.Various techniques have been prompted recently to recover optical imaging through turbid materials.Among them,speckle correlation based on deconvolution is one of the most attractive methods taking advantage of high imaging quality,robustness,eas-of-use,and ease-of-integration.By exploiting the point spread function(PSF)of the scattering system,large Field-of-View,extended Depth-of-Field,noninvasiveness and spectral resoluation are now available as successful solutions for high quality and multifunctional image reconstruction.In this paper,we review the progress of imaging through a scattering medium based on deconvolution method,including the principle,the breakthrough of the limitation of the optical memory ffect,the improvement of the deconvolution algorithm and innovative applications.Hexiang He Xiangsheng Xie Yikun Liu Haowen Liang Jianying Zhou 2019Journal of Innovative Optical Health Sciences2019,,4:0
15Cis-regulatory element-based genome-wide identification of DREB1/CBF targets in Arabidopsis显示文摘Microarray analysis is used to identify transcriptional targets. However, the direct targets of transcription factors cannot be distin- guished from indirect ones; further, genes with low-expression levels cannot be identi ed by this method. In the present study, we exploit the cis-element dehydration-responsive element (DRE) that is known to be responsible for the transcription of DRE binding factor 1 (DREB1) targets in the promoter region of all Arabidopsis genes. Putative targets whose promoters contain the elements were veri ed by both microarray and reverse transcription-polymerase chain reaction (RT-PCR) analysis. Five new DREB1/CBF direct targets were identi ed. Compared with traditional microarray analysis, our method is convenient and cost-e ective for identifying the downstream targets of transcription factors.Huan Zhao Weizhong Liu Yong Hu Xianglin Liu Yikun He 2008Progress in Natural Science:Materials International2008,18,5:0
16Transfer of a eubacteria-type cell division site-determining factor CrMinD gene to the nucleus from the chloroplast genome in Chlamydomonas reinhardtii显示文摘MinD is a ubiquitous ATPase that plays a crucial role in selection of the division site in eubacteria, chloroplasts, and probably Archaea. In four green algae, Mesostigma viride, Nephroselmis olivacea, Chlorella vulgaris and Prototheca wickerhamii, MinD homologues are encoded in the plastid genome. However, in Arabidopsis, MinD is a nucleus-encoded, chloroplast-targeted protein involved in chloro- plast division, which suggests that MinD has been transferred to the nucleus in higher land plants. Yet the lateral gene transfer (LGT) of MinD from plastid to nucleus during plastid evolution remains poorly understood. Here, we identified a nucleus-encoded MinD homologue from unicellular green alga Chlamydomonas reinhardtii, a basal species in the green plant lineage. Overexpression of CrMinD in wild type E. coli inhibited cell division and resulted in the filamentous cell formation, clearly demon- strated the conservation of the MinD protein during the evolution of photosynthetic eukaryotes. The transient expression of CrMinD-egfp confirmed the role of CrMinD protein in the regulation of plastid division. Searching all the published plastid genomic sequences of land plants, no MinD homologues were found, which suggests that the transfer of MinD from plastid to nucleus might have occurred be- fore the evolution of land plants.LIU WeiZhong HU Yong ZHANG RunJie ZHOU WeiWei ZHU JiaYing LIU XiangLin HE YiKun 2007Chinese Science Bulletin2007,52,18:0
17The mechanism of hydraulic fracturing assisted oil displacement to enhance oil recovery in low and medium permeability reservoirs显示文摘Aiming at the technology of hydraulic fracturing assisted oil displacement which combines hydraulic fracturing,seepage and oil displacement,an experimental system of energy storage and flowback in fracturing assisted oil displacement process has been developed and used to simulate the mechanism of percolation,energy storage,oil displacement and flowback of chemical agents in the whole process.The research shows that in hydraulic fracturing assisted oil displacement,the chemical agent could be directly pushed to the deeper area of the low and medium permeability reservoirs,avoiding the viscosity loss and adhesion retention of chemical agents near the pay zone;in addition,this technology could effectively enlarge the swept volume,improve the oil displacement efficiency,replenish formation energy,gather and exploit the scattered residual oil.For the reservoir with higher permeability,this measure takes effect fast,so to lower cost,and the high pressure hydraulic fracturing assisted oil displacement could be adopted directly.For the reservoir with lower permeability which is difficult to absorb water,hydraulic fracturing assisted oil displacement with surfactant should be adopted to reduce flow resistance of the reservoir and improve the water absorption capacity and development effect of the reservoir.The degree of formation energy deficit was the main factor affecting the effective swept range of chemical agents.Moreover,the larger the formation energy deficit was,the further the seepage distance of chemical agents was,accordingly,the larger the effective swept volume was,and the greater the increase of oil recovery was.Formation energy enhancement was the most important contribution to enhanced oil recovery(EOR),which was the key to EOR by the technology of hydraulic fracturing assisted oil displacement.LIU Yikun WANG Fengjiao WANG Yumei LI Binhui ZHANG Dong YANG Guang ZHI Jiqiang SUN Shuo WANG Xu DENG Qingjun XU He 2022Petroleum Exploration and Development2022,49,4:0
18A benzenesulfonic acid-modified organic polymer monolithic column with reversed-phase/hydrophilic bifunctional selectivity for capillary electrochromatography显示文摘Here,a styrene-based polymer monolithic column poly(VBS-co-TAT-co-AHM)with reversed-phase/hydrophilic interaction liquid chromatography(RPLC/HILIC)bifunctional separation mode was success-fully prepared for capillary electrochromatography by the in situ polymerization of sodium p-styrene sulfonate(VBS)with cross-linkers 3-(acryloyloxy)-2-hydroxypropyl methacrylate(AHM)and 1,3,5-triacryloylhexahydro-1,3,5-triazine(TAT).The preparation conditions of the monolith were optimized.The morphology and formation of the poly(VBS-co-TAT-co-AHM)monolith were confirmed by scanning electron microscopy(SEM)and Fourier transform infrared spectroscopy(FT-IR).The separation perfor-mances of the monolith were evaluated systematically.It should be noted that the incorporation of VBS functional monomer can provideπ-πinteractions,hydrophilic interactions,and ion-exchange in-teractions.Hence,the prepared poly(VBS-co-TAT-co-AHM)monolith can achieve efficient separation of thiourea compounds,benzene series,phenol compounds,aniline compounds and sulfonamides in RPLC or HILIC separation mode.The largest theoretical plate number for N,N0-dimethylthiourea reached 1.7×10^(5)plates/m.In addition,the poly(VBS-co-TAT-co-AHM)monolithic column showed excellent reproducibility and stability.This novel monolithic column has great application value and potential in capillary electrochromatography(CEC).Yikun Liu Ning He Yingfang Lu Weiqiang Li Xin He Zhentao Li Zilin Chen 2023Journal of Pharmaceutical Analysis2023,13,2:0
19An Improved Prediction Model of Vortex Shedding Noise from Blades of Fans显示文摘The main source of the noise of an axial flow fan is the fluctuating pressure field on blade surfaces caused by the shedding of vortices at the trailing edge of blades.An analytical model to predict the vortex shedding noise generated at the trailing edge of blades of axial flow fans was proposed by Lee in 1993.In this model,for mathematical convenience,an idealized vortex street is considered.However,the agreement between the analytical results and the experimental data needs to be improved because of the simplification about the Karman vortex street in the wake of blade.In the present study,a modified model is proposed based on the prediction model by Lee.The boundary layer theory is used to analyze and calculate the boundary layer development on both the pressure and the suction sides of blades.Considering the effect of boundary layer separation on the location of noise source,the predicted overall sound pressure level compares favorably with the experimental data of an axial fan.In the calculation of A-weighted sound pressure level(La),considering the effect of static pressure on radiate energy,the predicted broadband noise with the modified model compares favorably with the experimental data of a multiblade centrifugal fan.DOU Huashu LI Zhehong LIN Peifeng WEI Yikun CHEN Yongning CAO Wenbin HE Haijiang 2016Journal of Thermal Science2016,25,6:0
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