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| 1 | Discovery of mitochondrial chimeric-gene associated with cytoplasmic male sterility of HL-rice显示文摘The mitochondrial genome libraries of HL-type sterile line(A) and maintainer line(B) have been constructed. Mitochondrial gene, atp6, was used to screen libraries, due to the different Southern and Northern blot results between sterile and maintainer line. Sequencing analysis of positive clones proved that there were two copies of atp6 gene in sterile line and only one in maintainer line. One copy of atpt6 in sterile line was same to that in maintainer line; the other showed different flanking sequence from the 49th nucleotide downstream of the termination codon of atp6 gene. A new chimeric gene, orfH79, was found in the region. OrfH79 had homology to mitochondrial gene cox Ⅱ and orf107, and was special to HL-sterile cytoplasm. | Yl Ping WANG Li SUN Qingping ZHU Yingguo | 2002 | Chinese Science Bulletin2002，47，9： | 36 |
| 2 | Characterization and Use of Male Sterility in Hybrid Rice Breeding显示文摘引起那的杂交稻(Oryza sativa L.) 在导致的 the1970s 在中国被开始在米饭生产率的大改进。一般来说,它在超过 20% 增加谷物收益到生来的米饭变化,和现在杂交稻广泛地被介绍了进非洲,南部的亚洲和美洲。这些杂种品种通过也被产生三线的混合 andtwo 线混血儿系统;前者从细胞质雄性不育(厘米) 被导出,后者源于遗传因子的雄性不育性(公克) 。有厘米(HL, BT 和 WA ) 的三种主要类型和公克的二种类型(光周期敏感(PGMS ) 并且温度敏感(TGMS )) 。BT-typeCMS 和 HL-typeCMS 基因作为 orf79 和 orfH79 被描绘,它是妄想的有毒的基因导出的 frommitochondrial 重新整理。,为 CMS-WA 的 Rf3 位于染色体 1 Rf1, Rf4, Rf5 和 Rf6correspond 到 CMS-BT, CMS-WA 和 CMS-HL,在染色体 10 上定位了。为 BT 厘米的 Rf1 基因最近被克隆,并且编码指向线粒体的 PPR 蛋白质。PGMS 被认为被二后退的 loci 在染色体上控制 7 和 12,而九后退的等位基因为 TGMS 被识别了并且在不同染色体上印射。注意仍然着急地被需要解决雄性不育性的分子的复杂性帮助米饭繁殖…… | Shaoqing Li Daichang Yang Yingguo Zhu | 2007 | Journal of Integrative Plant Biology2007，49，6： | 33 |
| 3 | Mapping of the nuclear fertility restorer gene for HL cytoplasmic male sterility in rice using microsatellite markers显示文摘Bulked segregant analysis (BSA) of a BC1 population derived from Congguang 41A//Miyang 23/Congguang 41B was used to map the nuclear fertility restorer gene for Honglian (HL) cytoplasmic male sterility. One hundred and fifty-nine microsatellite primer pairs were screened for polymorphisms between the parents and between two bulks representing fertile and sterile plants. One microsatellite marker RM258 produced polymorphic products. The nuclear fertility restorer gene for HL cytoplasmic male sterility was mapped on chromosome 10, 7.8cM from RM258. The restorer gene may be clustered on chromosome. | HUANG Qingyang HE Yuqing JING Runchun ZHU Renshan ZHU Yingguo | 2000 | Chinese Science Bulletin2000，45，5： | 8 |
| 4 | Evaluation and optimization of blasting approaches to reducing oversize boulders and toes in open-pit mine显示文摘A new blasting approach of combined blastholes with different diameters is proposed to solve the problems of oversize boulders and rock toes in open-pit mine. A non-ideal detonation model and a statistical damage constitutive model are implemented in dynamic finite element analysis to investigate the formation mechanism of oversize boulders and toes. The damage distribution and evolution process of rock blasting fragmentation is simulated, and the scheme is further optimized. Numerical analysis results showed that pocket charges and satellite blastholes can only improve bench top fragmentation, but they cannot reduce the oversize in the middle and bottom of bench as well as the toe problem. The new blasting approach of combined blastholes with different diameters can effectively reduce the oversize boulders and toes as well as the production costs. | Zhendong Leng Yong Fan Qidong Gao Yingguo Hu | 2020 | International Journal of Mining Science and Technology2020，30，3： | 6 |
| 5 | Is a low dose of hepatitis B vaccine enough for a rapid vaccination scheme?显示文摘AIM: To determine whether or not a low dose of HB vaccine can be effectively used in the rapid vaccination.METHODS: Rapid vaccination (0, 1, 2 months) with low dose (5 μg) or routine dose (10 μg) HB vaccine was studied in 250 subjects (130 school children and 120 university students). Serum from all the participants was tested for HBsAg, anti-HBs and anti-HBc at 1, 3 and 7 months after the first dose of vaccination and all subjects were serum HBV marks negative before the vaccination. Non-responders to a complete initial vaccination from university students were given an additional vaccination with 10 μg of HB vaccine and their serum anti-HBs was tested again one month later.RESULTS: One month after the third dose of vaccination (third month) sero-conversion rates and geometric mean titer (GMTs) were significantly (P<0.01) higher in the routine dose (resp. 89 % and 106.8) than in the low dose group (resp. 72 % and 59.5). Sero-conversion rates and GMTs were maintained stable for another 4 months in both groups.After an additional vaccination to non-responders with 10 μg HB vaccine, 17/23 subjects (13/15 from those vaccinated with 5 μg vaccine and 4/8 from those vaccinated with 10 μg vaccine) became anti-HBs positive, yielding similar seroconversion rates for both dose groups.CONCLUSION: Higher sero-conversion rates and GMTs were reached in those vaccinated with 10 μg HB vaccine than in those vaccinated with 5 μg HB vaccine after a comp^te vaccination with a 0, 1, 2 month scheme. But the subjects vaccinated with 5 μg vaccine can also reach the similar sero-conversion rate after an additional vaccination. | Ru-XiangWang GreetBoland YingGuo Shao-PingLei Chang-HongYang JuanChen JieTian Jin-YingWen Ke-HongDu JanvanHattum GijsbertC.deGast | 2003 | World Journal of Gastroenterology2003，9，10： | 5 |
| 6 | Detection of bcl-2 and bax expression and bcl-2/JH fusion gene in intrahepatic cholangiocarcinoma显示文摘AIM: To investigate the relationship between bcl-2 gene and its related protein bax and intrahepatic cholangiocellular carcinoma (CCC). METHODS: Semi-nested in situ PCR (SNISPCR) and immunohistochemistry were performed to detect bcl-2/JH fusion gene and bcl-2, bax protein expression in 29 cases of CCC. RESULTS: No bcl-2/JH fusion gene was found in all cases of CCC, 72.4% of 29 cases expressed bcl-2 protein. Bcl-2 protein expression was related to histopathological grades (P<0.05). There was no corresponding relationship between bcl-2/JH fusion gene formation and bcl-2 protein expression in CCC (P<0.05). Bax was expressed in 10.3% of 29 cases. The ratio of bcl-2 to bax in normal liver tissues (3.5 to 1) was different from that in tumor tissues (7.0 to 1). CONCLUSION: It is suggested that bcl-2/JH fusion gene formation is not a frequent event and may not play an important role in the pathogenesis of CCC. However, aberrant ratio of bcl-2 to bax protein expression may be involved in the course of tumorigenesis of CCC. Abnormal bcl-2 protein expression may not be solely resulted from bcl-2/JH fusion gene. | Lin-LangGuo ShaXiao YingGuo | 2004 | World Journal of Gastroenterology2004，10，22： | 5 |
| 7 | Activation of transcription factors NF-kappaB and AP-1 and their relations with apoptosis-associated proteins in hepatocellular carcinoma显示文摘AIM: To study the distribution pattern of transcription factors NF-κB and AP-1 and their relations with the expression of apoptosis associated-proteins Fas/FasL and ICH-1L/S in human hepatocellular carcinoma (HCC).METHODS: We performed in situ hybridization and immunohistochemical techniques for NF-κB, AP-1,Fas/FasL and ICH-1 in 40 cases of human HCC along with corresponding nontumoral tissues and 7 cases of normal liver tissues.RESULTS: Twenty-two (55%) and 25 (62.5%) of 40 cases for NF-κB and AP-1 were presented for nuclear or both nuclear and cytoplastic staining respectively, while less cases were presented for only cytoplastic staining for NF-κB (18%) and AP-1 (10%) in adjacent nontumoral tissues and negative staining in normal liver tissues. There was no statistically significant difference of NF-κB or AP-1activation between well differentiated tumors and poorly differentiated tumors (P>0.05). NF-κB activity is positively corresponded to AP-1 activation. The expression of ICH-1L/S was associated with the activation of NF-κB and AP-1 (P<0.05), but no significant relationship was found between Fas/FasL and NF-κB or AP-1(P>0.05).CONCLUSION: Activation of both NF-κB and AP-1 may be required for ICH-1L/S-induced apoptosis in HCC, but not for Fas/FasL-mediated apoptosis. NF-κB and AP-1may play important roles in the pathogenesis of human HCC. | Lin-LangGuo ShaXiao YingGuo | 2005 | World Journal of Gastroenterology2005，11，25： | 4 |
| 8 | Effect of vector-expressed siRNA on HBV replication in hepatoblastoma cells显示文摘AIM: To study the effect of siRNA expressed from DNA vector on HBV replication.METHODS: Human U6 promoter was amplified from genomic DNA and cloned into plasmid pUC18 to construct a mammalian siRNA expression vector pUC18U6. Then oligonucleotides coding for a short hairpin RNA against HBV were cloned into pUC18U6 to form pUC18U6HBVsir which was introduced into 2.2.15 cells by using liposome-mediated transfection.2.2.15 cells transfected by pUC18U6 and pUC18U6GFPsir which expressed siRNA against green fluorescent protein and mock-transfected 2.2.15 cells were used as controls.Concentration of HBsAg in the supernatant of the transfected cells was measured by using solid-phase radioimmunoassay.RESULTS: A mammalian siRNA expression vector pUC18U6 was constructed successfully. Compared with controls,pUC18U6HBVsir which expressed siRNA against HBV decreased concentration of HBsAg significantly by 44%(P<0.05).CONCLUSION: HBV replication in 2.2.15 cells is inhibited by siRNA expressed from the DNA vector. | JunLiu YingGuo Cai-FangXue Ying-HuiLi Yu-XiaoHuang JinDing Wei-DongGong YaZhao | 2004 | World Journal of Gastroenterology2004，10，13： | 4 |
| 9 | The editing sites in transcripts of functional genes of rice mitochondria显示文摘RNA editing exists extensively in the higher plant mitochondria, and is a required step for forming functional proteins. There may be some relationship between RNA editing and cytoplasmic male sterility (CMS), a kind of phenomenon that is attributed to mitochondrial genome mutations. The research materials used are the gametophytic male sterility line (A), maintainer line (B) and F1 hybrid (F1) of HL-type CMS rice. cDNAs and DNAs of atp6 and coxII have been obtained from A, B and F1 by PCR and RT-PCR. Comparing sequences of cDNAs and DNAs, 18 and 15 editing sites were found respectively in the transcripts of atp6 and coxll. A, B and F1 shared the same editing sites. RNA editing improves hydrophobicity and conservation of the predicted protein as compared with other organisms. | Yl Ping WANG Li SUN Qingping ZHU Yingguo | 2002 | Chinese Science Bulletin2002，47，11： | 3 |
| 10 | Dam foundation excavation techniques in China:A review显示文摘A protective layer(PL) is commonly reserved above foundation surface to protect the underlying rock mass during dam foundation excavation. In China, the PL of dam foundation is conventionally subdivided into two or three thin layers and excavated with the shallow-hole blasting method, even by pneumatic pick method in case of soft rock mass. The aforementioned layered excavation of the PL delays the construction of the whole project. After nearly 30-year practices, several safe and effcient methods for the PL excavation of dam foundation are gradually developed. They include shallow-hole bench blasting with cushion material(SBC) at the bottom of the hole, and horizontal smooth blasting(HSB). The PL is even cancelled on the condition that horizontal pre-split technique is employed during dam foundation excavation. This paper introduces the aforementioned two PL excavation methods(shallow-hole blasting and bench blasting) and horizontal pre-split technique of dam foundation without protective layer(HPP). The basic principles of blasting method, blasting geometry, charge structure, drill-and-blast parameters of typical projects are examined. Meanwhile, the merits and limitations of each method are compared. Engineering practices in China show that HSB is basically the optimal method for dam foundation PL excavation in terms of foundation damage control and rapid construction. Some new problems for dam foundation PL excavation arising, such as strong unloading and relaxation phenomenon that encountered in the gorge region of southwest China, are needed to be addressed; and the corresponding countermeasures are discussed as well. | Yuzhu Zhang Wenbo Lu Ming Chen Peng Yan Yingguo Hu | 2013 | Journal of Rock Mechanics and Geotechnical Engineering2013，5，6： | 3 |
| 11 | Effect of vector-expressed shRNAs on hTERT expression显示文摘AIM: To study the effect of short hairpin RNAs (shRNAs)expressed from DNA vector on hTERT expression.METHODS: Oligonucleotides coding for four shRNAs against hTERT were cloned into a mammalian shRNA expressionvector pUC18U6 to form pUC18U6ht1-4, which were thenintroduced into HepG2 cells by using liposome-mediated transfection. HepG2 cells transfected by pUC18U6 and pUC18U6GFPsir, which expressed shRNA against green fluorescent protein (GFP), were used as controls. hTERT mRNA in the transfected cells were quantified by using real-time fluorescent RT-PCR.RESULTS: Among the four shRNAs against hTERT, two decreased the hTERT mRNA level. Compared with the controls, pUC18U6ht which expressed the two shRNAs reduced hTERT mRNA by 39% and 49% (P<0.05).CONCLUSION: hTERT expression is inhibited by the shRNAs expressed from the DNA vector. | YingGuo JunLiu Ying-HuiLi Tian-BaoSong JingWu Cai-XiaZheng Cai-FangXue | 2005 | World Journal of Gastroenterology2005，11，19： | 3 |
| 12 | Multi Points Updated and Distance Filtered Kriging Surrogate Model: Application in EOSS Optimization显示文摘 | LIU Xiaolu CHEN Yingwu CHEN Yingguo HE Renjie | 2013 | Chinese Journal of Electronics2013，22，1： | 2 |
| 13 | Defining reference genes for quantitative real-time PCR analysis of anther development in rice显示文摘量的即时聚合酶链反应(qPCR ) 是为基因 expressionanalysis 的最精确、广泛地使用的方法之一。然而,为正规化的引用基因的选择为 genomics 的基因 expression.As 开发的精确 quantification 是批评的,采矿大规模数据集象 microarray 和定序 RNA 那样数据成为新引用基因的新 approachfor 利用。在这研究,我们分析了包含不同纸巾和米饭花药和花粉的发展中的阶段的米饭花药和 167 microarraydatasets 的定序 RNA 数据集。我们选择了 5 参考基因,包括的 ACT1, eEF-1, GAPDH, Exp2,和 CCDC72 在以前的研究使用了的 12 候选人基因 andother,并且在八纸巾和花药 inrice 变化 9311 的不同发展阶段和 Yuetai 计算了他们的表达式。UPF3, eIF4A-3, GAPDH,和 PPP6 在米饭为花药开发的 qPCR 分析作为最合适的参考基因被识别。新候选人 referencegenes 比传统地使用的参考基因显示出更多的稳定的表示。这些结果在米饭花药为研究提供一套可靠 referencegenes 发展过程。 | Yanxiao Ji Ping Tu Kun Wang Feng Gao Weilong Yang Yingguo Zhu Shaoqing Li | 2014 | Acta Biochimica et Biophysica Sinica2014，46，4： | 2 |
| 14 | Unveiling the Effects of Interchain Hydrogen Bonds on Solution Gelation and Mechanical Properties of Diarylfluorene-Based Semiconductor Polymers显示文摘The intrinsically rigid and limited strain of most conjugated polymers has encouraged us to optimize the extensible properties of conjugated polymers.Herein,learning from the hydrogen bonds in glucose,which were facilitated to the toughness enhancement of cellulose,we introduced interchain hydrogen bonds to polydiarylfluorene by amide-containing side chains.Through tuning the copolymerization ratio,we systematically investigated their influence on the hierarchical condensed structures,rheology behavior,tensile performances,and optoelectronic properties of conjugated polymers.Compared to the reference copolymers with a low ratio of amide units,copolymers with 30%and 40%amide units present a feature of the shearthinning process that resembled the non-Newtonian fluid,which was enabled by the interchain dynamic hydrogen bonds.Besides,we developed a practical and universal method for measuring the intrinsic mechanical properties of conjugated polymers.We demonstrated the significant impact of hydrogen bonds in solution gelation,material crystallization,and thin film stretchability.Impressively,the breaking elongation for P4 was even up to~30%,which confirmed the partially enhanced film ductility and toughness due to the increased amide groups.Furthermore,polymer light-emitting devices(PLEDs)based on these copolymers presented comparable performances and stable electroluminescence(EL).Thin films of these copolymers also exhibited random laser emission with the threshold as low as 0.52μJ/cm^(2),suggesting the wide prospective application in the field of flexible optoelectronic devices. | Lubing Bai Yamin Han Chen Sun Xiang An Chuanxin Wei Wei Liu Man Xu Lili Sun Ning Sun Mengna Yu He Zhang Qi Wei Chunxiang Xu Yingguo Yang Tianshi Qin Linghai Xie Jinyi Lin Wei Huang | 2020 | Research2020，，1： | 2 |
| 15 | Origin of High Efficiency and Long-Term Stability in Ionic Liquid Perovskite Photovoltaic显示文摘Environment-friendly protic amine carboxylic acid ionic liquids(ILs)as solvents is a significant breakthrough with respect to traditional highly coordinating and toxic solvents in achieving efficient and stable perovskite solar cells(PSCs)with a simple one-step air processing and without an antisolvent treatment approach.However,it remains mysterious for the improved efficiency and stability of PSCs without any passivation strategy.Here,we unambiguously demonstrate that the three functions of solvents,additive,and passivation are present for protic amine carboxylic acid ILs.We found that the ILs have the capability to dissolve a series of perovskite precursors,induce oriented crystallization,and chemically passivate the grain boundaries.This is attributed to the unique molecular structure of ILs with carbonyl and amine groups,allowing for strong interaction with perovskite precursors by forming C=O…Pb chelate bonds and N-H…I hydrogen bonds in both solution and film.This finding is generic in nature with extension to a wide range of IL-based perovskite optoelectronics. | Lingfeng Chao Tingting Niu Hao Gu Yingguo Yang Qi Wei Yingdong Xia Wei Hui Shouwei Zuo Zhaohua Zhu Chengjie Pei Xiaodong Li Jing Zhang Junfeng Fang Guichuan Xing Hai Li Xiao Huang Xingyu Gao Chenxin Ran Lin Song Li Fu Yonghua Chen Wei Huang | 2020 | Research2020，，1： | 2 |
| 16 | A small biomoleculeassisted synthesis of iron sulfide nanostructures and magnetic properties 显示文摘 | MIN Yulin CHEN Youcun ZHAO Yingguo | 2009 | Solid State Sci2009，11，： | 1 |
| 17 | High-yield production of a low-temperature-active polygalacturonase for papaya juice clarification显示文摘 | Tao Tu Kun Meng Yingguo Bai Pengjun Shi Huiying Luo Yaru Wang Peilong Yang Yuhong Zhang Wei Zhang Bin Yao | 2013 | Food Chemistry2013，，3： | 1 |
| 18 | STABILITY AND BIFURCATION ANALYSIS OF A PREDATOR-PREY MODEL WITH CROWLEY-MARTIN TYPE FUNCTIONAL RESPONSE AND TIME DELAY显示文摘In this paper,a class of predator-prey model with Crowley-Martin type functional response and time delay is considered.By choosing the delay as a bifurcation parameter,it is shown that Hopf bifurcation occurs as the delay passes through a certain critical value.Some numerical simulations for verifying the main results are also provided. | Yingguo Li ,Junbin Ke(School of Math.and Computer Science,Fujian Normal University,Fuzhou 350007) | 2012 | Annals of Differential Equations2012，28，4： | 1 |
| 19 | Development of Maweizhan cytoplasmic male-sterile line in rice(Oryza sativa L.)显示文摘A male sterile mutant plant was found from the rice field of the local rice variety which was cultivated for a long time in the west of Hubei Province. The plant has more vigorous tillering ability. Its plant height of 95 cm was a bit shorter than that of the normal ones. | ZHU Yingguo YU Jinhong,and ZHANG Xiaoguo,College of Life Sci,Wuhan Univ,Wuhan 430072,China | 1996 | Chinese Rice Research Newsletter1996，4，4： | 1 |
| 20 | Identification of rice Di19 family reveals OsDi19 - 4 involved in drought resistance显示文摘 | Lili Wang Changchun Yu Cong Chen Chunlan He Yingguo Zhu Wenchao Huang | 2014 | Plant Cell Reports2014，，12： | 1 |
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