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| 1 | 草鱼呼肠孤病毒的三维重构与衣壳蛋白特性显示文摘草鱼呼肠孤病毒(grass carp reovirus,GCRV)为呼肠孤病毒科水生呼肠孤病毒属一新成员.最新的基因组序列分析发现,GCRV与哺乳动物呼肠孤病毒(mammalian reovirus,MRV)具有高度的同源性.为了解GCRV致病机理,进行了分辨率达到17?的三维重构与衣壳蛋白特性研究.结果表明:GCRV颗粒呈多层排列,包括RNA核心与内壳层、中间层及外壳层.由200个按T=13对称排列的三聚体组成外衣壳,其典型特征是在5次轴上出现三聚体缺失凹陷区,暴露出中间层三聚体亚单位.内壳层由120个单体组成,按T=1排列,结构特点与呼肠孤病毒科成员内衣壳特征相一致.衣壳蛋白电泳显示,GCRV颗粒含有7种蛋白(VP1-VP7)组分,与MRV衣壳蛋白特性相近,两者在衣壳结构组成上的相似性与基因组序列的高度同源性相吻合.此结果对进一步研究GCRV与宿主细胞相互作用机理具有指导意义. | 方勤 Sanket Shah Yuyao Liang Z.H.Zhou | 2005 | 中国科学(C辑)2005，35，3： | 14 |
| 2 | Three-dimensional structure of the wild-type RHDV显示文摘The three-dimensional (3D) structure of the wild-type rabbit hemorrhagic disease virus (RHDV) has been determined to a resolution of 3.2 nm by electron cryo-microscopy and computer image reconstruction techniques. The 3D density map exhibits characteristic structural features of a calicivirus: a T=3 icosahedral capsid with 90 arch-like capsomeres at the icosahedral and local 2-fold axes and 32 large surface hollows at the icosahedral 5- and 3-fold axes. This result confirms that the RHDV isolated in China is a member of the Caliciviridae family. A rather continuous capsid shell was found without channels. However, our RHDV structure also reveals some distinct structural char-acteristics not observed in other caliciviruses, including interconnected capsomeres and the lack of protuberance on the base of each of the surface hollows. Two types of particles were identified with similar outer capsid structure but different density distributions inside the capsid shells, which could not be distinguished by | ZHENG Dong XUE Tao CHEN Donghua ZHENG Ming Z.H.ZHOU XU Wei | 2001 | Chinese Science Bulletin2001，46，12： | 3 |
| 3 | Photovoltaic enhancement of Si solar cells by assembled carbon nanotubes显示文摘Photovoltaic conversion was enhanced by directly assemble of a network of single-walled carbon nanotubes(SWNTs) onto the surface of n-p junction silicon solar cells. When the density of SWNTs increased from 50 to 400 tubes μm^(-2), an enhancement of 3.92% in energy conversion efficiency was typically obtained. The effect of the SWNTs network is proposed for trapping incident photons and assisting electronic transportation at the interface of silicon solar cells. | Y.F.Zhang Y.F.Wang N.Chen Y.Y.Wang Y.Z.Zhang Z.H.Zhou L.M.Wei | 2010 | Nano-Micro Letters2010，2，1： | 3 |
| 4 | Three-dimensional structure of the inner core of rice dwarf virus显示文摘Rice dwarf virus (RDV) is a double-shelled icosahedral virus.Using electron cryomicroscopy and computer reconstruction techniques, we have determined a 3.3 nm resolution three-dimensional (3D) structure of the inner shell capsid without the outer shell and viral RNA. The results show that the inner shell is a thin, densely packed, smooth structure, which provides a scaffold for the full virus. A total of 120 copies of the major inner shell capsid protein P3 forms 60 dimers arranged in a T=1 icosahedral lattice. A close examination on the subunit packing of the T=1 inner core P3 with that of the T=13l outer shell P8 indicated that P8 trimers connect with P3 through completely non-equivalent, yet highly specific, intermolecular interactions. | 邵承华 Z.H.Zhou 卢光莹 | 2001 | Science China(Life Sciences)2001，44，2： | 1 |
| 5 | 伊蚊浓核病毒三维结构的比较分析显示文摘细小病毒是目前已知的结构最小的病毒,其宿主范围很广.利用冷冻电镜和三维重构技术获得了伊蚊浓核病毒1.2nm分辨率下的三维结构,并利用计算机生物信息处理技术和氨基酸序列比对技术比较了该病毒和其他细小病毒的结构和序列的异同.尽管均属于浓核病毒亚科,但是无论从衣壳结构还是其蛋白质的氨基酸序列上,伊蚊浓核病毒和其他昆虫的细小病毒都有很大差异.相比之下,伊蚊浓核病毒和人类B19细小病毒的衣壳蛋白相似性较大,两者的结构蛋白和非结构蛋白一致性也高于伊蚊浓核病毒与其他昆虫的细小病毒的一致性.此结果表明:伊蚊浓核病毒和人类B19细小病毒有着密切关系,前者可能来源于B19病毒的较近期变种. | 程凌鹏 陈森雄 Z.H.Zhou 张景强 | 2006 | 中国科学(C辑)2006，36，4： | 1 |
| 6 | 新颖的超高真空CVD外延设备与低温低压硅外延研究显示文摘为实现低温低压外延新工艺,建立了一台具有多项原位监控功能的新颖的超高真空CVD(chemicalvapordeposition)外延设备,并用此设备,(100)和(111)硅片分别在550℃和650℃下由硅烷热解法成功地生长了硅外延层。高分辨率的横断面透射电镜(XTEM)照片表明,低温硅外延层中存在一些缺陷,这些缺陷都由衬底表面引起的,然后扩展进入外延层。此外,衬底{100}Si和{111}Si因晶向不同,不仅获得外延层所需的最低温度不同,而且外延层中的缺陷特征差异也很大。 | 叶志镇 Z.H.Zhou R.Reif | 1994 | 真空科学与技术1994，14，5： | 0 |
| 7 | Three-dimensional Structure of Rice Dwarf Virus by Electron Cryomicroscopy显示文摘The three-dimensional(3D)structure of intact rice dwarf virus(RDV)has been deter-mined to 2.6 nm resolution by 400kV electron cryomicroscopy and computer reconstructiontechniques.The intact virion of 69.8 nm in diameter was seen to be made up of an outer shellof 6.9 nm in thickness and an inner shell of 2.5 nm in thickness at a diameter of 54 nm.Theouter shell surface forms a T=13 icosahedral lattice with holes at 5-and 6-coordinated posi-tions.The 780 capsomeres at local 3-fold positions appear trimeric.The interface betweenthese two shells could be clearly delineated and the interaction was maintained by a cylindri-cal density whose upper domain connects subunits of the trimeric capsomeres by an interlock-ing mechanism.The 3D structure of inner shell was also reconstructed to 3.3 nm from100kV images of purified inner shell particles,confirming our interpretation of the multilayerstructure of intact RDV. | Lu Guangying~(①②) Z.H.Zhou~② J.Jakana~② Cai Deyou~① Chen Shengxiang~③ Wei Xincheng~① Gu Xiaocheng~① W.Chiu~② (①College of Life Sciences,Peking University,Beijing 100871,P.R.China) (②W.M.Keck Center for Computational Biology and Verna & Marts McLean Dept.of Biochem.,Baylor College of Medicine,Houston,TX 77030,USA) (③Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,P.R.China) | 1995 | High Technology Letters1995，1，1： | 0 |
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