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| 1 | Expression of miRNA-140 in Chondrocytes and Synovial Fluid of Knee Joints in Patients with Osteoarthritis显示文摘Objective To investigate the expression of miRNA-140 in chondrocytes and synovial fluid of osteoarthritis(OA) patients, and explore the relationship between the miRNA-140 expression and OA severity. Methods This study enrolled 30 OA patients who underwent total knee arthroplasty for chondrocytes sampling and 30 OA patients who underwent intra-articular injection for synovial fluid sampling. All OA patients were grouped into mild [Kellgren and Lawrence(KL) grade 1-2], moderate(KL grade 3) and severe(KL grade 4), with 10 in each subgroups for each sampling purposes. 7 non-OA patients and 10 patients with knee injury were collected for cartilage and synovial fluid sampling respectively as control groups. Chondrocytes were isolated from the cartilage tissue and cultured in vitro. Quantitative real time PCR for miRNA-140 in chondrocytes and synovial fluid were performed, and the U6 sn RNA was used as internal control. The expression difference of miRNA-140 among groups and correlation between the expression and the KL grade of OA were analysed using one-way ANOVA and Spearman test respectively. Results The expression of miRNA-140 in chondrocytes of knees in OA patients was reduced than that in normal knees, and the between-group difference was statistically significant(F=305.464, P<0.001). miRNA-140 could be detected in synovial fluid of both normal knees and OA knees, its relative expression level was reduced in synovial fluid of OA group compared with normal group, and the between-group difference was statistically significant as well(F=314.245, P<0.001). The relative expression level of miRNA-140 in both chondrocytes and synovial fluid were negatively correlated with the KL grade of OA(r=-0.969, P<0.001; r=-0.970, P<0.001). Conclusion miRNA-140 could be detected in chondrocytes and synovial fluid of OA patients, and its expression was negatively correlated with the severity of OA. | Hai-bo Si Yi Zeng Zong-ke Zhou Fu-xing Pei Yan-rong Lu Jing-qiu Cheng Bin Shen | 2016 | Chinese Medical Sciences Journal2016，31，4： | 9 |
| 2 | Lotus seed skin proanthocyanidin extract exhibits potent antioxidant property via activation of the Nrf2-ARE pathway显示文摘Lotus seed is well known as traditional food and medicine,but its skin is usually discarded.Recent studies have shown that lotus seed skin contains a high concentration of proanthocyanidins that have multi-functions,such as antioxidation,anti-inflammation,and anti-cancer effects.In the present study,we aimed to isolate and purify the proanthocyanidins from lotus seed skin by acetone extraction and rotary evaporation,identify their chemical structures by HPLC-MS-MS and NMR,and further investigate the antioxidant properties of the extract purified by macroporous resin (PMR) from lotus seed skin both in vitro and in vivo.The results showed that PMR mainly contained oligomeric proanthocyanidins,especially dimeric procyanidin B1 (PB1),procyanidin B2 and procyanidin B4.Although it had limited ability to directly scavenge radicals in vitro,PMR could significantly enhance the expressions of antioxidant proteins via activation of nuclear factory-related factor 2 (Nrf2)-antioxidant response element (ARE) pathway in HepG2 cells.Molecular data revealed that PB1,a major component in PMR,stabilized Nrf2 by inhibiting the ubiquitination of Nrf2,which led to subsequent activation of the Nrf2-ARE pathway,including the enhancements of Nrf2 nuclear translocation,Nrf2-ARE binding and ARE transcriptional activity.Moreover,the in vivo results in high fat diet-induced mice further verified the powerful antioxidant property of PMR.These results revealed that lotus seed skin is a promising resource for functional food development. | Tao Li Qili Li Weiguo Wu Yong Li De-xing Hou Hua Xu Baodong Zheng Shaoxiao Zeng Yang Shan Xiangyang Lu Fangming Deng Si Qin | 2019 | Acta Biochimica et Biophysica Sinica2019，51，1： | 8 |
| 3 | Protective effect of heme oxygenase-1 on lung injury induced by erythrocyte instillation in rats显示文摘血的背景 Intratracheal 灌输导致自我修理的尖锐的肺 injury.However ,修理的机制是 unclear.Heme-oxygenase (惊讶) -1,which 催化 heine 故障,对在这研究客观的 inflammation.The 的一个重要角色是在 intratracheal 引起红 cells.Methods 的灌输的肺损害测试 HO-1 的角色的氧化应力和戏充当可诱导的防卫四十只健康、男 Sprague-Dawley 老鼠随机被划分成五个 groups:normal 组, | PANG Qing-feng ZHOU Qiao-mei ZENG Si DOU Li-dong JI Yong ZENG Yin-ming | 2008 | Chinese Medical Journal2008，，17： | 8 |
| 4 | Suppressed expression of miR-378 targeting gzmb in N cells is required to control dengue virus infection显示文摘 | Shuyan Liu Lingming Chen Ying Zeng Lulu Si Xiaolan Guo Junmei Zhou Danyun Fang Gucheng Zeng Lifang Jiang | 2016 | Cellular & Molecular Immunology2016，13，5： | 7 |
| 5 | Protective effects of heat shock protein70 induced by geranyl-geranylacetone in atrophic gastritis in rats显示文摘瞄准:在老鼠和它的潜在的机制在衰退胃炎的前进上调查 geranylgeranylacetone (GGA ) 的效果。方法:衰退胃炎与 24 个星期的 0.1% 氨答案, 60% 乙醇,和 20 mmol/Ldeoxycholic 酸在 Sprague-Dawley 老鼠被导致。由衰退胃炎的正式就职伴随了, 200 mg/kg GGA 被口头的 gavage 管理 8 个星期(星期 17-24 ) 。在胃的 mucosawere 的组织学的变化由 1 公里的腺的发炎,胃的 mucosal 厚度,和数量的索引测定了在窦的水平长度。70 铺平的内长的热吃惊蛋白质(HSP ) 和分发被弄污的免疫和免疫组织化学在胃的 mucosa 决定。结果: GGA 与发炎地势减轻了衰退胃炎的病理学的前进(发炎索引:1.40 在 GGA 组织, 1.65 在衰退胃炎组织) 并且腺的恢复(mucosal 厚度和腺的数量:在 GGA 组的 194.3 妈妈和 38.7 公里;在衰退胃炎组的 123.3mum 和 32.7 公里;P < 0.05 ) 。GGA 显著地导致了 HSP70 合成(P < 0.05 ) 。而且,橡黄素, HSP70 表示的一个禁止者,在窦加重了煽动性的房间和腺的损失的渗入。结论:GGA 经由 HSP70 表示的正式就职在老鼠阻止了衰退胃炎的前进。 | Wei-li LIU Shu-jie CHEN Yan CHEN Lei-min SUN Wei ZHANG Ya-min ZENG Tian-hua ZHOU Jian-min SI | 2007 | Acta Pharmacologica Sinica2007，28，7： | 7 |
| 6 | Promoting hepatic growth factor in the treatment of heavy type hepatitis and severe chronic hepatitis: a multicenter clinical study显示文摘BACKGROUND: The mortality rate of heavy type hepatitisis high. No special treatment is available except generaltreatment. This multicenter clinical study was designed toobserve the safety and efficacy of promoting hepatic growthfactor (PHGF) in the treatment of heavy type hepatitis andsevere chronic hepatitis.METHODS: 347 patients with heavy type hepatitis and 324with severe chronic hepatitis were subjected to administra-tion of 120 μg of PHGF per day for 4 weeks on the basis ofgeneral treatment. Those who were being effectively treat-ed would last additional 2 to 4 weeks. Blood routine, urineroutine, blood urea nitrogen (BUN), blood creatinine(Cr), blood ammonia, alpha fetoprotein (AFP), electro-lyte, alanine transaminase (ALT), aspartate transaminase(AST), serum total bilirubin (TBIL), serum direct biliru-bin (DBIL), prothrombin time activity (PTA), total pro-tein (TP) and albumin (ALB) were detected in the pa-tients before treatment, 2 weeks after treatment, and at theend of the treatment. Any side-effect would be recorded.RESULTS: In the patients with severe chronic hepatitis, thetotal effective rate of the treatment was 88. 9% The levelsof ALT, AST and TBIL decreased significantly (P<0.001),whereas those of PTA and ALB increased significantly (P <0.001), and the level of AFP increased slightly. In patientswith heavy type hepatitis, the total effective rate of thistreatment was 78.4%, and patients at different stage showeddifferent results. The total effective rates of patients withearly, medium and terminal stage heavy type hepatitis were89.9%, 84.8% and 27.5%, respectively. No severe side-effect was shown.CONCLUSION: PHGF is effective and safe in the treat-ment of patients with heavy type hepatitis and severe chro-nic hepatitis. But it should be administered early in patientswith heavy type hepatitis so as to get better curative effects. | Jing-Hang Xu, Yan-Yan Yu, Chong-Wen Si, Zheng Zeng, Qin-Huan Wang, Duan-De Luo,Yong-Xing Zhou, Min-De Zeng, Guang-Yan Qiao, Ji-Lu Yao and Wei-Lun Lu Department of Infectious Diseases, First Hospital ofBeijing University, Beijing 100034, China Department of Gastroenterology, Wuhan UnionHospital, Wuhan 430022, China Department of Gastroentero-logy , Affiliated Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, China Department of Gastroenterology, RenjiHospital, Shanghai 200001 , China Department of InfectiousDiseases, Second Affiliated Hospital, China Medical University, Shenyang110004, China Department of Gastroenterology, Third Affilia-ted Hospital, China Medical University, Shenyang 110004 , China | 2004 | Hepatobiliary & Pancreatic Diseases International2004，3，3： | 7 |
| 7 | 863 genomes reveal the origin and domestication of chicken显示文摘Despite the substantial role that chickens have played in human societies across the world,both the geographic and temporal origins of their domestication remain controversial.To address this issue,we analyzed 863 genomes from a worldwide sampling of chickens and representatives of all four species of wild jungle fowl and each of the five subspecies of red jungle fowl(RJF).Our study suggests that domestic chickens were initially derived from the RJF subspecies Gallus gallus spadiceus whose present-day distribution is predominantly in southwestern China,northern Thailand and Myanmar.Following their domestication,chickens were translocated across Southeast and South Asia where they interbred locally with both RJF subspecies and other jungle fowl species.In addition,our results show that the White Leghorn chicken breed possesses a mosaic of divergent ancestries inherited from other subspecies of RJF.Despite the strong episodic gene flow from geographically divergent lineages of jungle fowls,our analyses show that domestic chickens undergo genetic adaptations that underlie their unique behavioral,morphological and reproductive traits.Our study provides novel insights into the evolutionary history of domestic chickens and a valuable resource to facilitate ongoing genetic and functional investigations of the world's most numerous domestic animal. | Ming-Shan Wang Mukesh Thakur Min-Sheng Peng Yu Jiang Laurent Alain Francois Frantz Ming Li Jin-Jin Zhang Sheng Wang Joris Peters Newton Otieno Otecko Chatmongkon Suwannapoom Xing Guo Zhu-Qing Zheng Ali Esmailizadeh Nalini Yasoda Hirimuthugoda Hidayat Ashari Sri Suladari Moch Syamsul Arifin Zein Szilvia Kusza Saeed Sohrabi Hamed Kharrati-Koopaee Quan-Kuan Shen Lin Zeng Min-Min Yang Ya-Jiang Wu Xing-Yan Yang Xue-Mei Lu Xin-Zheng Jia Qing-Hua Nie Susan Joy Lamont Emiliano Lasagna Simone Ceccobelli Humpita Gamaralalage Thilini Nisanka Gunwardana Thilina Madusanka Senasige Shao-Hong Feng Jing-Fang Si Hao Zhang Jie-Qiong Jin Ming-Li Li Yan-Hu Liu Hong-Man Chen Cheng Ma Shan-Shan Dai Abul Kashem Fazlul Haque Bhuiyan Muhammad Sajjad Khan Gamamada Liyanage Lalanie Pradeepa Silva Thi-Thuy Le Okeyo Ally Mwai Mohamed Nawaz Mohamed Ibrahim Megan Supple Beth Shapiro Olivier Hanotte Guojie Zhang Greger Larson Jian-Lin Han Dong-Dong Wu Ya-Ping Zhang | 2020 | Cell Research2020，30，8： | 7 |
| 8 | Rumen fermentation and bacterial communities in weaned Chahaer lambs on diets with different protein levels显示文摘We evaluated the effects of diets with different crude protein(CP) levels on growth performance, rumen fermentation and bacterial communities in weaned Chahaer lambs. 128 weaned Chahaer lambs((20.56±1.43) kg body weight; ram:ewe 1:1) aged(61±1.85) d were randomly allotted to one of four diets with CP content of 11.17%(T1), 12.06%(T2), 13.40%(T3) or 14.36%(T4). Ruminal fermentation parameters were measured and bacterial communities were analysed using PCR-denaturing gradient gel electrophoresis(PCR-DGGE) and quantitative PCR. The average daily gain and feed utilization efficiency in T3 were higher than those in the other groups(P<0.05), although the dry matter intake and metabolizable energy intake were similar. Total volatile fatty acid concentration in the ruminal fluid of T3 was lower than that of T1(P=0.011), T2(P=0.008) or T4(P=0.309). The ammonia nitrogen concentration and acetate/propionate ratio of ruminal fluid were significantly higher in lambs fed the higher CP diets, whereas the molar concentrations of propionate and butyrate of ruminal fluid were lower. The rumen bacterial community was similar in T2 and T3 which shown more stable and diverse rumen microbes ecosystem compared with the other groups. The DGGE profiles and phylogenetic tree indicated that Bacteroides uniformis, Clostridium alkalicellulosi, Alkalibaculum bacchi and Saccharofermentans sp. were common bacterium of Chahaer lamb rumen. B. uniformis, C. alkalicellulosi, Saccharofermentans sp. and Gracilibacter thermotolerans, which belong to the Bacteroidetes and Firmicutes phyla, were the dominant species in the rumen of lambs fed 13.40% CP. However, Ruminococcus albus, Ruminococcus flavefaciens and Butyrivibrio fibrisolvens were not different in lambs fed different CP diets. Therefore, it could be concluded that B. uniformis, C. alkalicellulosi, A. bacchi and Saccharofermentans sp. were common bacteria of Chahaer lamb rumen. Furthermore, the dietary CP of 13.04% could improve performance and change rumen fermentation model by increasing the dominant species' peak intensities of B. uniformis, C. alkalicellulosi, Saccharofermentans sp. and Gracilibacter thermotolerans and stabilizing rumen microbial ecosystem. | YANG Chun-tao SI Bing-wen DIAO Qi-yu JIN Hai ZENG Shu-qin TU Yan | 2016 | Journal of Integrative Agriculture2016，15，7： | 6 |
| 9 | Effects of antiviral agents and HBV genotypes on intrahepaticcovalently closed circular DNA in HBeAg-positive chronichepatitis B patients显示文摘AIM:To evaluate the effects of antiviral agents and HBV genotypes on intrahepatic covalently closed circular DNA (ccc DNA) in HBeAg-positive chronic hepatitis B patients. METHODS:Seventy-one patients received lamivudine (n = 35), or sequential therapy with lamivudine-interferon alpha 2b (IFN-α 2b, n = 24) for 48 wk, or IFN-α 2b (n = 12) for 24 wk. All subjects were followed up for 24 wk. Intrahepatic ccc DNA was measured quantitatively by PCR. HBV genotypes were analyzed by PCR-RFLP. RESULTS:Sequential lamivudine-INF-α therapy, lamivudine and INF-α monotherapy reduced ccc DNA of 1.7 log, 1.4 log and 0.8 log, respectively (P < 0.05). Seventeen out of the 71 patients developed HBeAg seroconversion, the reduction of ccc DNA in the HBeAg seroconversion patients was more significant than that in the HBeAg positive patients (3.0 log vs 1.6 log, P = 0.0407). Twenty-four weeks after antiviral therapy withdrawal, 16 patients had a sustained virological response, the baseline intrahepatic ccc DNA in the patients with a sustained virological response was significantly lower than that in the patients with virological rebound (4.6 log vs 5.4 log, P = 0.0472). HBV genotype C accounted for 85.9% (n = 61), and genotype B for 14.1% (n = 10), respectively, in the 71 patients. There was no significant difference in the change of ccc DNA level between HBV genotypes C and B (2.1 log vs 1.9 log). CONCLUSION:Forty-eight week sequential lamivudine-INF-α therapy and lamivudine monotherapy reduce ccc DNA more significantly than 24-wk INF-α monotherapy. Low baseline intrahepatic ccc DNA level may predict the long-term efficacy of antiviral treatment. HBV genotypes C and B have no obvious influence on ccc DNA load. | Hai-Ying Lu Li-Wei Zhuang Yan-Yan Yu Chong-Wen Si Jun Li Jian-Jun Zhang Zheng Zeng Xin-Yue Chen Zhong-Hou Han Yong Chen | 2008 | World Journal of Gastroenterology2008，14，8： | 6 |
| 10 | Modulation of expression of P16 and Her2 in rat breast tissues of mammary hyperplasia model by external use of Rupifang Extract显示文摘OBJECTIVE:To observe the effect of Rupifang Extract in external use on expression of proto-oncogenes her2 and tumor suppression genes p16 in rat breast tissues of mammary hyperplasia model.To explore the mechanisms of Rupifang Extract in external use for preventing and treating mammary hyperplasia.METHODS:Thirty virginal female Wistar rats were randomized into 5 groups,6 in each,A:blank control group;B:model group;C:the low dose group of Rupifang;D:the middle dose group of Rupifang;and E:The high dose group of Rupifang.The mammary hyperplasia rat models were produced by injecting estradiol benzoate and progesterone and irritating by tail nipping.Drug intervention was also launched during the model formation.After 30 days,the expression of her2 and p16 in breast tissues of rats in each group were detected by the SP immunohistochemical method.RESULTS:Compared with Blank control group,the expression of her2 in breast tissues in Model group was higher,and the expression of p16 was lower(P<0.05 or P<0.01).After intervention with Rupifang Extract,compared with Model group,the expression of her2 in breast tissues in Rupifang groups was lower,and the expression of p16 higher(P<0.05 or P<0.01).CONCLUSION:The mechanisms of Rupifang Extract in external application for preventing and treating mammary hyperplasia may be reducing the expression of proto-oncogenes her2 and increasing the expression of tumor suppression genes p16. | Guijuan Zhang Dehui Li Hui Guo Rui Liao Bizhu Tan Suyi Zhang Yubin Liu Min Ma Xiaoting Zeng Zhuolong Peng Si Huang Yinghui Zheng Yi Ma | 2012 | Journal of Traditional Chinese Medicine2012，32，4： | 6 |
| 11 | Virtual reality-based rehabilitation in patients following total knee arthroplasty:a systematic review and meta-analysis of randomized controlled trials显示文摘Background:Physical therapy is regarded as an essential aspect in achieving optimal outcomes following total knee arthroplasty(TKA).The coronavirus disease 2019(COVID-19)pandemic has made face-to-face rehabilitation inaccessible.Virtual reality(VR)is increasingly regarded as a potentially effective option for offering health care interventions.This systematic review and meta-analysis investigate VR-based rehabilitation's effectiveness on outcomes following TKA.Methods:From inception to May 22,2021,PubMed/Medline,Embase,Web of Science,the Cochrane Central Register of Controlled Trials,Scopus,PsycINFO,Physiotherapy Evidence Database,China National Knowledge Infrastructure,and Wanfang were comprehensively searched to identify randomized controlled trials(RCTs)evaluating the effect of VR-based rehabilitation on patients following TKA according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses statement and the Cochrane Handbook for Systematic Reviews of Interventions.Results:Eight studies were included in the systematic review,and seven studies were included in the meta-analysis.VR-based rehabilitation significantly improved visual analog scale(VAS)scores within 1 month(standardized mean difference[SMD]:−0.44;95%confidence interval[CI]:−0.79 to−0.08,P=0.02),the Western Ontario and McMaster Universities Osteoarthritis Index(WOMAC)within 1 month(SMD:−0.71;95%CI:−1.03 to−0.40,P<0.01),and the Hospital for Special Surgery Knee Score(HSS)within 1 month and between 2 months and 3 months(MD:7.62;95%CI:5.77 to 9.47,P<0.01;MD:10.15;95%CI:8.03 to 12.27,P<0.01;respectively)following TKA compared to conventional rehabilitation.No significant difference was found in terms of the Timed Up and Go(TUG)test.Conclusions:VR-based rehabilitation improved pain and function but not postural control following TKA compared to conventional rehabilitation.More high-quality RCTs are needed to prove the advantage of VR-based rehabilitation.As the COVID-19 pandemic continues,it is necessary to promote this rehabilitation model. | Linbo Peng Yi Zeng Yuangang Wu Haibo Si Bin Shen | 2022 | Chinese Medical Journal2022，，2： | 5 |
| 12 | ERK1/2-mediated Cytoplasmic Accumulation of hnRNPK Antagonizes TRAIL-induced Apoptosis through Upregulation of XIAP in H1299 Cells显示文摘Objective Tumor necrosis factor-related apoptosis-inducing ligand(TRAIL) resistance greatly limits the clinical therapeutic efficacy of TRAIL. Elucidating the molecular mechanism underlying TRAIL resistance will be fundamental to resolving this problem. Methods Nuclear and cytoplasmic protein extraction and immunofluorescence(IF) assay were used to detect changes in heterogeneous nuclear ribonucleoprotein K(hn RNPK) localization in H1299 cells. The evaluation of cell apoptosis in cells transfected with GFP-hn RNPK, GFP-hn RNPK S284/353 A, or GFP-hn RNPK S284/353 D mutant was performed using cleaved caspase-3 antibody. The gene expression of XIAP was tested by quantitative RT-PCR. Results Previously, we reported that hn RNPK antagonized TRAIL-induced apoptosis through inhibition of PKC-mediated GSK3β phosphorylation. In this study, we further demonstrate that TRAIL treatment induces cytoplasmic accumulation of hn RNPK in H1299 cells. The hn RNPK localized in the cytoplasm has a higher capacity to antagonize TRAIL-induced apoptosis. Both ERK1/2 signaling inhibitor U0126 and ERK-phosphoacceptor-site mutant(GFP-hn RNPK S284/353A) diminish cytoplasmic accumulation of hn RNPK induced by TRAIL. Moreover, we show that XIAP is involved in hn RNPK-mediated TRAIL resistance in H1299 cells. Conclusion Taken together, these results give new insights into the understanding of the molecular mechanism associated with TRAIL resistance in lung adenocarcinoma. | HUANG Wen Si XU Feng Mei ZENG Qing Zhong LIU Xiao Hui GAO Xue Juan LIU Lang Xia | 2017 | Biomedical and Environmental Sciences2017，30，7： | 5 |
| 13 | Cardiac fibroblast heat shock protein 47 aggravates cardiac fibrosis post myocardial ischemia-reperfusion injury by encouraging ubiquitin specific peptidase 10 dependent Smad4 deubiquitination显示文摘Despite complications were significantly reduced due to the popularity of percutaneous coronary intervention(PCI) in clinical trials, reperfusion injury and chronic cardiac remodeling significantly contribute to poor prognosis and rehabilitation in AMI patients. We revealed the effects of HSP47 on myocardial ischemia-reperfusion injury(IRI) and shed light on the underlying molecular mechanism.We generated adult mice with lentivirus-mediated or miRNA(mi1/133TS)-aided cardiac fibroblastselective HSP47 overexpression. Myocardial IRI was induced by 45-min occlusion of the left anterior descending(LAD) artery followed by 24 h reperfusion in mice, while ischemia-mediated cardiac remodeling was induced by four weeks of reperfusion. Also, the role of HSP47 in fibrogenesis was evaluated in cardiac fibroblasts following hypoxia-reoxygenation(HR). Extensive HSP47 was observed in murine infarcted hearts, human ischemic hearts, and cardiac fibroblasts and accelerated oxidative stress and apoptosis after myocardial IRI. Cardiac fibroblast-selective HSP47 overexpression exacerbated cardiac dysfunction caused by chronic myocardial IRI and presented deteriorative fibrosis and cell proliferation.HSP47 upregulation in cardiac fibroblasts promoted TGFβ1-Smad4 pathway activation and Smad4 deubiquitination by recruiting ubiquitin-specific peptidase 10(USP10) in fibroblasts. However, cardiac fibroblast specific USP10 deficiency abolished HSP47-mediated fibrogenesis in hearts. Moreover, blockage of HSP47 with Col003 disturbed fibrogenesis in fibroblasts following HR. Altogether, cardiac fibroblast HSP47 aggravates fibrosis post-myocardial IRI by enhancing USP10-dependent Smad4 deubiquitination,which provided a potential strategy for myocardial IRI and cardiac remodeling. | Saiyang Xie Yun Xing Wenke Shi Min Zhang Mengya Chen Wenxi Fang Shiqiang Liu Tong Zhang Xiaofeng Zeng Si Chen Shasha Wang Wei Deng Qizhu Tang | 2022 | Acta Pharmaceutica Sinica B2022，12，11： | 3 |
| 14 | Lupeol protects against cardiac hypertrophy via TLR4-PI3K-Akt-NF-κB pathways显示文摘Inflammation and apoptosis are main pathological processes that lead to the development of cardiac hypertrophy.Lupeol,a natural triterpenoid,has shown anti-inflammatory and anti-apoptotic activities as well as potential protective effects on cardiovascular diseases.In this study we investigated whether lupeol attenuated cardiac hypertrophy and fibrosis induced by pressure overload in vivo and in vitro,and explored the underlying mechanisms.Cardiac hypertrophy was induced in mice by transverse aortic constriction(TAC)surgery,and in neonatal rat cardiomyocytes(NRCMs)by stimulation with phenylephrine(PE)in vitro.We showed that administration of lupeol(50 mg·kg^(-1)·d^(-1),i.g.,for 4 weeks)prevented the morphological changes and cardiac dysfunction and remodeling in TAC mice,and treatment with lupeol(50μg/mL)significantly attenuated the hypertrophy of PE-stimulated NRCMs,and blunted the upregulated hypertrophic markers ANP,BNP,andβ-MHC.Furthermore,lupeol treatment attenuated the apoptotic and inflammatory responses in the heart tissue.We revealed that lupeol attenuated the inflammatory responses including the reduction of inflammatory cytokines and inhibition of NF-κB p65 nuclear translocation,which was mediated by the TLR4-PI3K-Akt signaling.Administration of a PI3K/Akt agonist 740 Y-P reversed the protective effects of lupeol in TAC mice as well as in PE-stimulated NRCMs.Moreover,pre-treatment with a TLR4 agonist RS 09 abolished the protective effects of lupeol and restored the inhibition of PI3K-Akt-NF-κB signaling by lupeol in PE-stimulated NRCMs.Collectively,our results demonstrate that the lupeol protects against cardiac hypertrophy via anti-inflammatory mechanisms,which results from inhibiting the TLR4-PI3K-Akt-NF-κB signaling. | Dan Li Ying-ying Guo Xian-feng Cen Hong-liang Qiu Si Chen Xiao-feng Zeng Qian Zeng Man Xu Qi-zhu Tang | 2022 | Acta Pharmacologica Sinica2022，43，8： | 3 |
| 15 | Intrahepatic HBV DNA as a predictor of antivirus treatment efficacy in HBeAg-positive chronic hepatitis B patients显示文摘AIM: To evaluate the effect of antiviral agents on intrahepatic HBV DNA in HBeAg-positive chronic hepatitis B patients. METHODS: Seventy-one patients received treatment with lamivudine,interferon alpha (IFN-α2b) or sequential therapy with lamivudine-IFN-α2b for 48 wk. All subjects were followed up for 24 wk. Serum and intrahepatic HBV DNA were measured quantitatively by PCR. HBV genotypes were analyzed by PCR-RFLP. RESULTS: At the end of treatment,the intrahepatic HBV DNA level in 71 patients decreased from a mean of (6.1 ± 1.0) log10 to (4.9 ± 1.4) log10. Further,a larger decrease was seen in the intrahepatic HBV DNA level in patients with HBeAg seroconversion. Intrahepatic HBV DNA level (before and after treatment) was not significantly affected by the patients' HBV genotype,or by the probability of virological flare after treatment. CONCLUSION: Intrahepatic HBV DNA can be effectively lowered by antiviral agents and is a significant marker for monitoring antivirus treatment. Low intrahepatic HBV DNA level may achieve better efficacy of antivirus treatment. | Hai-Ying Lu Li-Wei Zhuang Yan-Yan Yu Hadad Ivan Chong-Wen Si Zheng Zeng Jun Li Dong-Ming Hou Xin-Yue Chen Zhong-Hou Han Yong Chen | 2007 | World Journal of Gastroenterology2007，13，20： | 3 |
| 16 | Weighted Estimates for Iterated Commutators of Multilinear Fractional Operators显示文摘The author establishes weighted strong type estimates for iterated commutators of multilinear fractional operators. | Zeng Yan SI Shan Zhen LU | 2012 | Acta Mathematica Sinica,English Series2012，28，9： | 3 |
| 17 | λ-Central BMO Estimates for Commutators of Multilinear Maximal Operators显示文摘The authors establish λ-central BMO estimates for commutators of maximal multilinear Calderón-Zygmund operators TΠb* and multilinear fractional operators Iα,b on central Morrey spaces respectively. Similar results still hold for Tb,Tb* and Iα,b*. | Zeng Yan SI Qing Ying XUE | 2013 | Acta Mathematica Sinica,English Series2013，29，4： | 2 |
| 18 | Examine the Correlation between Heat Shock Protein IbpA and Heat Tolerance in Cronobacter sakazakii显示文摘We used a proteomic approach to identify Ibp A in Cronobacter sakazakii(C. sakazaki), which is related to heat tolerance in this strain. The abundance of Ibp A in C. sakazakii strains strongly increased after heat shock. C. sakazakii CMCC45402 ibpA deletion mutants were successfully constructed. The C. sakazakii CMCC 45402 ΔibpA and wild-type strains could not be distinguished based on colony morphology on LB agar plates or biochemical assays. The growth of the C. sakazaki CMCC 45402 ΔibpA mutant in heat shock conditions was indistinguishable from that of the isogenic wild-type, but showed greater heat resistance than E. coli O157:H7 strain CMCC 44828. This study suggests that the absence of a single ibpA gene has no obvious effect on the phenotype or heat resistance of the strain C. sakazakii CMCC 45402. | ZHAO Zhi Jing WANG Bin YUAN Jing LIANG Hao Yu DONG Si Guo ZENG Ming | 2017 | Biomedical and Environmental Sciences2017，30，8： | 2 |
| 19 | LMP2-DC Vaccine Elicits Specific EBV-LMP2 Response to Effectively Improve Immunotherapy in Patients with Nasopharyngeal Cancer显示文摘Objective To evaluate the safety and effectiveness of a vaccine based on latent membrane protein 2(LMP2)modified dendritic cells(DCs)that boosts specific responses of cytotoxic T lymphocytes(CTLs)to LMP2 before and after intradermal injection in patients with nasopharyngeal carcinoma(NPC).Methods DCs were derived from peripheral blood monocytes of patients with NPC.We prepared LMP2-DCs infected by recombinant adenovirus vector expressing LMP2(rAd-LMP2).NPC patients were immunized with 2×105 LMP2-DCs by intradermal injection at week 0 and after the second and fourth weeks.Specific responses to LMP2 were detected by enzyme-linked immunospot(ELISPOT)assay at week 0 and at the fifth and eighth weeks.Local clinicians performed the follow-up and tracking of patients.Results We demonstrated that DCs derived from monocytes displayed typical DC morphologies;the expression of LMP2 in the LMP2-DCs vaccine was confirmed by immunocytochemical assay.Twenty-nine patients with NPC were enrolled in this clinical trial.The LMP2-DCs vaccine was well tolerated in all of the patients.Boosted responses to LMP2 peptide sub-pools were observed in 18 of the 29 patients with NPC.The follow-up data of 29 immunized patients from April,2010 to April 2015 indicated a five-year survival rate of 94.4%in responders and 45.5%in non-responders.Conclusion In this pilot study,we demonstrated that the LMP2-DCs vaccine is safe and effective in patients with NPC.Specific CTLs responses to LMP2 play a certain role in controlling and preventing the recurrence and metastasis of NPC,which warrants further clinical testing. | ZENG Yi SI Yong Feng LAN Gui Ping WANG Zhan ZHOU Ling TANG Min Zhong SJ O`Brien LAN Jiao ZHOU Xiang Yang WANG Yong Li TANG Juan ZHOU Zhi Xiang DU Hai Jun LIN Hui | 2020 | Biomedical and Environmental Sciences2020，33，11： | 2 |
| 20 | λ-Central BMO Estimates for Multilinear Commutators of Fractional Integrals显示文摘作者建立中央 BMO 为 multilinear 的整流器估计的位 - 在中央 Morrey 空格的部分不可分的操作员。关键词位 - 中央 BMO 空格 - 中央 Morrey 空格 - multilinear 部分操作符先生(2000 ) 题目分类 42B20 - 42B35 由中国(资助号码 10871024 ) | Zeng Yan SI | 2010 | Acta Mathematica Sinica,English Series2010，26，11： | 1 |
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