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| 1 | Isolation and phylogenetic assignation of actinomycetes in the marine sediments from the Arctic Ocean显示文摘Actinomycetes in five marine sediments collected from the Arctic Ocean at depths of 43 to 3 050 m were cultivated using a variety of media. A total of 61 actinomycete colonies with substrate mycelia only were observed, and no colonies with aerial mycelia were observed under aerobic conditions at 15 ℃. From these colonies, 28 were selected to represent different morphological types.Denaturing gradient gel electrophoresis(DGGE) was used to check the purity of isolates and select representatives for subsequent sequencing. Phylogentic analyses based on nearly full-length 16S ribosomal RNA gene (rDNA) sequences indicated that the actinomycetes isolated were accommodated within genus Rhodococcus of family Nocardiaceae, genus Dietzia of family Dietziaceae, genera Janibacter and Terrabacter of family Instrasporangiaceae and genera Kocuria and Arthrobacter of family Micrococcaceae. One of the strains(P27-24) from the deep-sea sediment at depth of 3 050 m was found to be identical in 16S rDNA sequence(1474/1474)with the radiation-resistant Kocuria rosea ATCC 187T isolated from air. More than half of the isolates showed the similarities ranging from 99.5% to 99.9% in 16S rDNA sequence to dibenzofran-degrading, butyl 2-ethylhexanoate-hydrolysising and nitrile-metabolizing actinomycetes. All the strains isolated were psychrotolerant bacteria and grew better on the media prepared with natural seawater than on the media prepared with deionized water. Three of them (Dietzia sp. P27-10, Rhodococcus sp. S11-3 and Rhodococcus sp.P11-5) had an obligate growth requirement for salt, confirming that these strains are indigenous marine actinomycetes. | YU Yong LI Huirong ZENG Yinxin CHEN Bo | 2005 | Acta Oceanologica Sinica2005，24，6： | 8 |
| 2 | Molecular genetic diversity of bacteria in the bottom section of arctic sea ice from the Canada Basin显示文摘PCR-DGGE approach was used to analyze bacterial diversity in the bottom section of seven arctic sea ice samples colleted from the Canada Basin. Thirty-two 16S rDNA sequences were obtained from prominent DGGE bands. The closest relatives of these sequences are found to be those of cultivated or uncultured bacteria from antarctic or arctic sea ice. Phylogenetic analysis clustered these sequences or phylotypes within α- proteobacteria, γ-proteobacteria and CFB (cytophaga-flexibacter-bacteroides) group. Sequences belonging to γ-proteobacteria were dominant and members of the CFB group were highly abundant. It was suggested that the CFB group was the representative of the bottom section of sea ice samples. | LI Huirong YU Yong CHEN Bo ZENG Yinxin REN Daming | 2005 | Acta Oceanologica Sinica2005，24，6： | 6 |
| 3 | Isolation and characteristics of one marine psychrotrophic cellulase-generating bacterium Ar/w/b/75°/10/5 from Chuckchi Sea,Arctic显示文摘Microorganisms living in polar zones play an important part as the potential source of organic activity materials with low temperature characteristics in the biotechnological applications. A psychrotrophic bacterium (strain Ar/w/b/75°/10/5), producing cellulase at low temperatures during late-exponential and early-stationary phases of cell growth, was isolated from sea ice-covered surface water in Chuckchi Sea, Arctic. This bacterium, with rod cells, was Gram-negative, slightly halophilic. Colony growing on agar plate was in black. Optimum growth temperature was 15℃. No cell growth was observed at 35℃ or above. Optimum salt concentration for cell growth was between 2 and 3 % of sodium chloride in media. Maximal cellulase activity was detected at a temperature of 35℃ and pH8. Cellulase was irreversibly inactivated when incubated at 55℃ within 30 min. Enzyme can be kept stable at the temperature no higher than 25℃. Of special interest was that this bacterium produced various extracellular enzymes including cellulase, amylase, agar hydrolase and protease, at low or moderate temperature conditions, which is certainly of it potential value for applications. | Zeng Yinxin(曾胤新) Chen Bo(陈波) | 2002 | Chinese Journal of Polar Science2002，13，2： | 4 |
| 4 | Phylogenetic diversity of dimethylsulfoniopropionate-dependent demethylase gene dmdA in distantly related bacteria isolated from Arctic and Antarctic marine environments显示文摘Dimethylsulfoniopropionate(DMSP) is mainly produced by marine phytoplankton as an osmolyte, antioxidant,predator deterrent, or cryoprotectant. DMSP is also an important carbon and sulfur source for marine bacteria.Bacteria may metabolize DMSP via the demethylation pathway involving the DMSP demethylase gene(dmdA) or the cleavage pathway involving several different DMSP lyase genes. Most DMSP released into seawater is degraded by bacteria via demethylation. To test a hypothesis that the high gene frequency of dmdA among major marine taxa results in part from horizontal gene transfer(HGT) events, a total of thirty-one bacterial strains were isolated from Arctic Kongsfjorden seawater in this study. Analysis of 16S rRNA gene sequences showed that,except for strains BSw22118, BSw22131 and BSw22132 belonging to the genera Colwellia, Pseudomonas and Glaciecola, respectively, all bacteria fell into the genus Pseudoalteromonas. DmdA genes were detected in five distantly related bacterial strains, including four Arctic strains(Pseudoalteromonas sp. BSw22112, Colwellia sp.BSw22118, Pseudomonas sp. BSw22131 and Glaciecola sp. BSw22132) and one Antarctic strain(Roseicitreum antarcticum ZS2–28). Their dmdA genes showed significant similarities(97.7%–98.3%) to that of Ruegeria pomeroyi DSS–3, which was originally isolated from temperate coastal seawater. In addition, the sequence of the gene transfer agent(GTA) capsid protein gene(g5) detected in Antarctic strain ZS2–28 exhibited a genetically closely related to that of Ruegeria pomeroyi DSS–3. Among the five tested strains, only Pseudomonas sp. BSw22131 could grow using DMSP as the sole carbon source. The results of this study support the hypothesis of HGT for dmdA among taxonomically heterogeneous bacterioplankton, and suggest a wide distribution of functional gene(i.e., dmdA) in global marine environments. | Yinxin Zeng | 2019 | Acta Oceanologica Sinica2019，38，8： | 3 |
| 5 | Diversity of the aerobic anoxygenic phototrophy gene pufM in Arctic and Antarctic coastal seawaters显示文摘Aerobic anoxygenic phototrophic(AAP) bacteria serve important functions in marine carbon and energy cycling because of their capability to utilize dissolved organic substrates and harvest light energy.AAP bacteria are widely distributed in marine environments,and their diversity has been examined in marine habitats.However,information about AAP bacteria at high latitudes remains insufficient to date.Therefore,this study determined the summer AAP bacterial diversity in Arctic Kongsfjorden and in the Antarctic coastal seawater of King George Island on the basis of puf M,a gene that encodes a pigment-binding protein subunit of the reaction center complex.Four puf M clone libraries were constructed,and 674 positive clones were obtained from four investigated stations(two in Kongsfjorden and two in the Antarctic Maxwell Bay).Arctic clones were clustered within the Alphaproteobacteria,whereas Antarctic clones were classified into the Alphaproteobacteria and Betaproteobacteria classes.Rhodobacteraceae-like puf M genes dominated in all samples.In addition,sequences closely related to puf M encoded on a plasmid in Sulfitobacter guttiformis were predominant in both Arctic and Antarctic samples.This result indicates the transpolar or even global distribution of puf M genes in marine environments.Meanwhile,differences between the Arctic and Antarctic sequences may prove polar endemism.These results indicate the important role of Rhodobacteraceae as AAP bacteria in bipolar coastal waters. | ZENG Yinxin DONG Peiyan QIAO Zongyun ZHENG Tianling | 2016 | Acta Oceanologica Sinica2016，35，6： | 3 |
| 6 | Phylogenetic diversity and phenotypic characterization of cultivable bacterioplankton isolated from polar oceans显示文摘A set of 27 marine planktonic bacteria isolated from the polar regions was characterized by 16S rDNA sequencing and physiological and biochemical testing. More than half of these bacteria were positive for caseinase, gelatinase and β-glucosidase, and could utilize glucose, maltose or malic acid as carbon source for cell growth. Twelve isolates expressed nitrate reduction activities. Except for one antarctic isolate BSw10175 belonging to Actinobacteria phylum, these isolates were classified as γ-Proteobacteria, suggesting that γ-Proteobacteria dominated in cultivable marine bacterioplankton at both poles. Genus Pseudoalteromonas was the predominant group in the Chukchi Sea and the Bering Sea, and genus Shewanella dominated in cultivable bacterioplankton in the Prydz Bay. With sequence similarities above 97%, genus Psychrobacter was found at both poles. These 27 isolates were psychrotolerant, and significant 16S rDNA sequence similarities were found not only between arctic and antarctic marine bacteria (>99%), but also between polar marine bacteria and bacteria from other aquatic environments (≥98.8%), including temperate ocean, deep sea, pond and lake, suggesting that in the polar oceans less temperature-sensitive bacteria may be cosmopolitan and have a bipolar, even global, distribution at the species level. | ZENG Yinxin LI Huirong YU Yong CHEN Bo ZHENG Tianling | 2007 | Acta Oceanologica Sinica2007，26，4： | 1 |
| 7 | Diversity of bacterioplankton in coastal seawaters of Fildes Peninsula, King George Island, Antarcti- ca显示文摘 | Zeng Yinxin Yu Yong Qiao Zongyun | 2014 | Archives of Microbiology2014，196，2： | 1 |
| 8 | Phylogenetic diversity of planktonic bacteria in the Chukchi Borderland region in summer显示文摘Planktonic bacteria are abundant in the Chukchi Borderland region. However, little is known about their diversity and the roles of various bacteria in the ocean. Seawater samples were collected from two stations K2S and K4S where sea ice was melting obviously. The analysis of water samples with fluorescence in situ hybridization (FISH) showed that DMSP-degrading bacteria accounted for 13% of the total bacteria at the station K2S. No aerobic anoxygenic phototrophic (AAP) bacteria were detected in both samples. The bacterial communities were characterized by two 16S rRNA gene clone libraries. Sequences fell into four major lineages of the domain Bacteria, including Proteobacteria (Alpha, Beta and Gamma subclasses), Bacteroidetes, Actinobacteria and Firmicutes. No significant difference was found between the two clone libraries. SAR11 and Rhodobacteraceae clades of Alphaproteobacteria and Pseudoalteromonas of Gammapro-teobacteria constituted three dominant fractions in the clone libraries. A total of 191 heterotrophic bacterial strains were isolated and 76% showed extracellular proteolytic activity. Phylogenetic analysis reveals that the isolates fell into Gammaproteobacteria, Bacteroidetes, Actinobacteria and Firmicutes. The most common genus in both the bacterial isolates and protease-producing bacteria was Pseudoalteromonas. UniFrac data showed suggestive differences in bacterial communities between the Chukchi Borderland and the northern Bering Sea. | ZENG Yinxin YU Yong LI Huirong HE Jianfeng LEE Sang H SUN Kun | 2013 | Acta Oceanologica Sinica2013，32，6： | 1 |
| 9 | Diversity of bacteria in surface ice of Austre Lov6nbreen glacier, Svalbard 显示文摘 | Zeng Yinxin Yan Ming Yu Yong | 2013 | Ar- chives of Microbiology2013，195，5： | 1 |
| 10 | Spontaneous decoration of ionic compounds at perovskite interfaces to achieve 23.38% efficiency with 85% fill factor in NiO_X-based perovskite solar cells显示文摘Inorganic hole transport materials, particularly NiO_X, have shown considerable promise in boosting the efficiency and stability of perovskite solar cells. However, a major barrier to commercialization of NiO_X-based perovskite solar cells with positive-intrinsic-negative architectures is their direct contact with the absorbing layer, which can lead to losses of photovoltage and fill factor. Furthermore, highly positive under-coordinated Ni cations degrade the perovskite at the interface. Here, we address these issues with the use of an ionic compound(QAPyBF_(4)) as an additive to passivate defects throughout the perovskite layer and improve carrier conduction and interactions with under-coordinated Ni cations. Specifically,the highly electronegative inorganic anion [BF_(4)]~- interacts with the NiO_x/perovskite interface to passivate under-coordinated cations(Ni^(≥3+)). Accordingly, the decorated cells achieved a power conversion efficiency of 23.38% and a fill factor of 85.5% without a complex surface treatment or NiO_X doping. | Geping Qu Deng Wang Xiaoyuan Liu Ying Qiao Danish Khan Yinxin Li Jie Zeng Pengfei Xie Yintai Xu Peide Zhu Limin Huang Yang-Gang Wang Baomin Xu Zong-Xiang Xu | 2023 | Journal of Energy Chemistry2023，，10： | 0 |
| 11 | Preliminary genome analysis of psychrotolerant marine bacterium Pseudoalteromonas sp. BSw20308 reveals its potential applications显示文摘The genus Pseudoalteromonas is ubiquitous in the marine environment and can synthesize a wide range of extracellular compounds.Psychrotolerant Pseudoalteromonas sp.BSw20308 was isolated from the Chukchi Sea,a marginal sea of the Arctic Ocean.It produces a number of extracellular enzymes that can degrade polysaccharides and proteins.The BSw20308 genome was sequenced to 38.1-fold coverage,and the sequences were assembled into 146 contigs(≥500 bp).In total,4 172 open reading frames(ORFs)with an average gene length of 987 bp were detected.At least 86 ORFs were predicted by sequence analysis to encode a variety of catalytic modules involved in the degradation of polysaccharides,proteins,and lipids.In addition,36 ORFs were predicted to encode catalytic modules involved in the degradation of organic pollutants and halogenated compounds,and in the production of bioactive compounds.The draft genome sequence of BSw20308 provides new information about the ecological function and adaptation of the genus Pseudoalteromonas in Arctic marine environments,and also indicates its potential applications in the biotechnology industries(e.g.,enzymology,and pollutant degradation). | ZENG Yinxin YU Yong QIAO Zongyun LI Huirong LUO Wei | 2013 | Advances in Polar Science2013，24，4： | 0 |
| 12 | Phylogenetic relationship and phenotypic comparison of Psychrobacter species isolated from polar oceans显示文摘在极的海洋,四 Psychrobacter 紧张, BSw10170, BSw20352, BSw20370,和 BSw20461 调查 bacterioplankton 的种系发生的关系和生物地理学,从 Bering 海, Chukchi 海,和 Prydz 海湾的海水孤立,被定序的 16S rDNA 描绘并且生理、生物化学严峻。结果证明靠近的关系比 97% 高与顺序类似在北极和南极紧张之间存在。这四 Psythrobcicter 紧张不仅在他们之中显示出几乎相同的 phenotypic 特征,而且与那些相关 Psychrobacter 种分享了很多类似,显示那 psychrotolerance 和 Psythrobacterstrains 的光圈忍耐可能在为他们在在类水平的海洋的环境的双极的、甚至全球的分发的原因之中。 | ZENG Yinxin LI Huirong YU Yong CHEN Bo | 2007 | Progress in Natural Science:Materials International2007，17，1： | 0 |
| 13 | Relationships between two Pseudoalteromonas strains isolated from the Canada Basin and the Southern Ocean using a polyphasic approach显示文摘The evolutionary relationships and taxonomic position of two marine planktonic bacterial strains BSw20211 and BSw10014,isolated from the Canada Basin and from the Southern Ocean,respectively,were determined using a polyphasic taxonomic approach.There was a close phylogenetic relationship between the two strains and most phenotypic properties were shared.Nonetheless,they were found to belong to different species of the genus Pseudoalteromonas on the basis of genotypic analyses.Findings were consistent with the suggestion that gyrB gene sequence comparison and DNA-DNA relatedness might better define phylogenetic relationships of bacteria at the species level.However,a cut-off value of 90% gyrB gene sequence similarity was not reliable for the differentiation of species within the genus Pseudoalteromonas. | Zeng YinXin Zheng TianLing | 2011 | Chinese Journal of Polar Science2011，22，1： | 0 |
| 14 | Complete genome analysis of bacteriochlorophyll acontaining Roseicitreum antarcticum ZS2-28^(T)reveals its adaptation to Antarctic intertidal environment显示文摘Aerobic anoxygenic phototrophic bacteria(AAPB)are photoheterotrophic prokaryotes able to use both light and dissolved organic matter as energy sources.Roseicitreum antarcticum ZS2-28^(T)was isolated from intertidal sediment in the Larsemann Hills,Princess Elizabeth Land,Antarctica,and was able to produce bacteriochlorophyll a.It is the type strain of the sole species within the genus Roseicitreum.The complete genome sequence of the bacterium was determined using Illumina HiSeq X and PacBio RSII systems.The genome of R.antarcticum ZS2-28^(T)was 4253095 bp and consisted of one chromosome and four plasmids.A number of genes related to the bacteriochlorophyll a production,photosynthetic reaction,cold adaptation,salt adaptation,ultra-violet resistance and DNA damage repairing were found in the genome.In addition to genomic islands and typeⅣsecretion systems,genes related to gene transfer agents were detected in the genome of R.antarcticum ZS2-28^(T),suggesting that this bacterium can adapt to its environment by acquiring exogenous nucleic acids.The annotated complete genome sequence provides genetic insights into the environmental adaptation and ecological function of R.antarcticum ZS2-28^(T)in Antarctic coastal area. | ZENG Yinxin YU Yong LI Huirong LUO Wei DING Haitao | 2021 | Advances in Polar Science2021，32，1： | 0 |
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