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| 1 | Blunted nitric oxide regulation in Tibetans under high-altitude hypoxia显示文摘Nitric oxide(NO) is an important molecule for vasomotor tone, and elevated NO signaling was previously hypothesized as a unique and adaptive physiological change in highland Tibetans. However, there has been lack of NO data from Tibetans living at low altitude and lowlander immigrants living at high altitude, which is crucial to test this hypothesis. Here, through cross-altitude(1990–5018 m) and cross-population(Tibetans and Han Chinese) analyses of serum NO metabolites(NOx) of 2086 individuals, we demonstrate that although Tibetans have a higher serum NOx level compared to lowlanders, Han Chinese immigrants living at high altitude show an even higher level than Tibetans. Consequently, our data contradict the previous proposal of increased NO signaling as the unique adaptive strategy in Tibetans.Instead, Tibetans have a relatively lower circulating NOx level at high altitude. This observation is further supported by data from the hypoxic experiments using human umbilical vein endothelial cells and gene knockout mice. No difference is detected between Tibetans and Han Chinese for endothelial nitric oxide synthase(e NOS), the key enzyme for circulating NO synthesis, suggesting that e NOS itself is unlikely to be the cause. We show that other NO synthesis-related genes(e.g. GCH1) carry Tibetan-enriched mutations significantly associated with the level of circulating NOx in Tibetans. Furthermore, gene network analysis revealed that the downregulation and upregulation of NOx is possibly achieved through distinct pathways.Collectively, our findings provide novel insights into the physiological and genetic mechanisms of the evolutionary adaptation of Tibetans to high-altitude hypoxia. | Yaoxi He Xuebin Qi Ouzhuluobu Shiming Liu Jun Li Hui Zhang Baimakangzhuo Caijuan Bai Wangshan Zheng Yongbo Guo Duojizhuoma Baimayangji Dejiquzong Bianba Gonggalanzi Yongyue Pan Qula Kangmin Cirenyangji Wei Guo Yangla Yi Peng Xiaoming Zhang Kun Xiang Zhaohui Yang Liangbang Wang Gengdeng Yanfeng Zhang Tianyi Wu Bing Su Chaoying Cui | 2018 | National Science Review2018，5，4： | 7 |
| 2 | Comparative genomic and transcriptomic analyses uncover the molecular basis of high nitrogen-use efficiency in the wheat cultivar Kenong 9204显示文摘Studying the regulatory mechanisms that drive nitrogen-use efficiency(NUE)in crops is important for sustainable agriculture and environmental protection.In this study,we generated a high-quality genome assembly for the high-NUE wheat cultivar Kenong 9204 and systematically analyzed genes related to nitrogen uptake and metabolism.By comparative analyses,we found that the high-affinity nitrate transporter gene family had expanded in Triticeae.Further studies showed that subsequent functional differentiation endowed the expanded family members with saline inducibility,providing a genetic basis for improving the adaptability of wheat to nitrogen deficiency in various habitats.To explore the genetic and molecular mechanisms of high NUE,we compared genomic and transcriptomic data from the high-NUE cultivar Kenong 9204(KN9204)and the low-NUE cultivar Jing 411 and quantified their nitrogen accumulation under high-and low-nitrogen conditions.Compared with Jing 411,KN9204 absorbed significantly more nitrogen at the reproductive stage after shooting and accumulated it in the shoots and seeds.Transcriptome data analysis revealed that nitrogen deficiency clearly suppressed the expression of genes related to cell division in the young spike of Jing 411,whereas this suppression of gene expression was much lower in KN9204.In addition,KN9204 maintained relatively high expression of NPF genes for a longer time than Jing 411 during seed maturity.Physiological and transcriptome data revealed that KN9204 was more tolerant of nitrogen deficiency than Jing 411,especially at the reproductive stage.The high NUE of KN9204 is an integrated effect controlled at different levels.Taken together,our data provide new insights into the molecular mechanisms of NUE and important gene resources for improving wheat cultivars with a higher NUE trait. | Xiaoli Shi Fa Cui Xinyin Han Yilin He Long Zhao Na Zhang Hao Zhang Haidong Zhu Zhexin Liu Bin Ma Shusong Zheng Wei Zhang Jiajia Liu Xiaoli Fan Yaoqi Si Shuiquan Tian Jianqing Niu Huilan Wu Xuemei Liu Zhuo Chen Deyuan Meng Xiaoyan Wang Liqiang Song Lijing Sun Jie Han Hui Zhao Jun Ji Zhiguo Wang Xiaoyu He Ruilin Li Xuebin Chi Chengzhi Liang Beifang Niu Jun Xiao Junming Li Hong-Qing Ling | 2022 | Molecular Plant2022，15，9： | 6 |
| 3 | Mesenchymal stem cell treatment improves outcome of COVID-19 patients via multiple immunomodulatory mechanisms显示文摘The infusion of coronavirus disease 2019(COVID-19)patients with mesenchymal stem cells(MSCs)potentially improves clinical symptoms,but the underlying mechanism remains unclear.We conducted a randomized,single-blind,placebo-controlled(29 patients/group)phase II clinical trial to validate previous findings and explore the potential mechanisms.Patients treated with umbilical cord-derived MSCs exhibited a shorter hospital stay(P=0.0198)and less time required for symptoms remission(P=0.0194)than those who received placebo.Based on chest images,both severe and critical patients treated with MSCs showed improvement by day 7(P=0.0099)and day 21(P=0.0084).MSC-treated patients had fewer adverse events.MSC infusion reduced the levels of C-reactive protein,proinflammatory cytokines,and neutrophil extracellular traps(NETs)and promoted the maintenance of SARS-CoV-2-specific antibodies.To explore how MSCs modulate the immune system,we employed single-cell RNA sequencing analysis on peripheral blood.Our analysis identified a novel subpopulation of VNN2+hematopoietic stem/progenitorlike(HSPC-like)cells expressing CSF3R and PTPRE that were mobilized following MSC infusion.Genes encoding chemotaxis factors—CX3CR1 and L-selectin—were upregulated in various immune cells.MSC treatment also regulated B cell subsets and increased the expression of costimulatory CD28 in T cells in vivo and in vitro.In addition,an in vivo mouse study confirmed that MSCs suppressed NET release and reduced venous thrombosis by upregulating kindlin-3 signaling.Together,our results underscore the role of MSCs in improving COVID-19 patient outcomes via maintenance of immune homeostasis. | Rongjia Zhu Tingdong Yan Yingmei Feng Yan Liu Hongcui Cao Gongxin Peng Yanlei Yang Zhen Xu Jingqi Liu Wei Hou Xiaoyue Wang Zhe Li Luchan Deng Shihua Wang Jing Li Qin Han Hongling Li Guangliang Shan Yinghao Cao Xingyan An Jianshe Yan Zhonghui Zhang Huafei Li Xuebin Qu Jiaqi Zhu Shumin Zhou Jiao Wang Fengchun Zhang Jinming Gao Ronghua Jin Dayong Xu Yan-Qing Ma Tao Huang Shuang Peng Zhi Zheng Ilia Stambler Eric Gilson Lee Wei Lim Alexey Moskalev Antonio Cano Sasanka Chakrabarti Brun Ulfhake Huanxing Su Haoying Xu Sihuan Xu Feng Wei Holly MBrown-Borg Kyung-Jin Min Georgina Ellison-Hughes Calogero Caruso Kunlin Jin Robert Chunhua Zhao | 2021 | Cell Research2021，31，12： | 5 |
| 4 | De novo assembly of a Tibetan genome and identification of novel structural variants associated with high-altitude adaptation显示文摘Structural variants(SVs) may play important roles in human adaptation to extreme environments such as high altitude but have been under-investigated. Here, combining long-read sequencing with multiple scaffolding techniques, we assembled a high-quality Tibetan genome(ZF1), with a contig N50 length of 24.57 mega-base pairs(Mb) and a scaffold N50 length of 58.80 Mb. The ZF1 assembly filled 80 remaining N-gaps(0.25 Mb in total length) in the reference human genome(GRCh38). Markedly, we detected17 900 SVs, among which the ZF1-specific SVs are enriched in GTPase activity that is required for activation of the hypoxic pathway. Further population analysis uncovered a 163-bp intronic deletion in the MKL1 gene showing large divergence between highland Tibetans and lowland Han Chinese. This deletion is significantly associated with lower systolic pulmonary arterial pressure, one of the key adaptive physiological traits in Tibetans. Moreover, with the use of the high-quality de novo assembly, we observed a much higher rate of genome-wide archaic hominid(Altai Neanderthal and Denisovan) shared non-reference sequences in ZF1(1.32%–1.53%) compared to other East Asian genomes(0.70%–0.98%),reflecting a unique genomic composition of Tibetans. One such archaic hominid shared sequence-a662-bp intronic insertion in the SCUBE2 gene-is enriched and associated with better lung function(the FEV1/FVC ratio) in Tibetans. Collectively, we generated the first high-resolution Tibetan reference genome, and the identified SVs may serve as valuable resources for future evolutionary and medical studies. | Ouzhuluobu Yaoxi He Haiyi Lou Chaoying Cui Lian Deng Yang Gao Wangshan Zheng Yongbo Guo Xiaoji Wang Zhilin Ning Jun Li Bin Li Caijuan Bai Baimakangzhuo Gonggalanzi Dejiquzong Bianba Duojizhuoma Shiming Liu Tianyi Wu Shuhua Xu Xuebin Qi Bing Su | 2020 | National Science Review2020，7，2： | 4 |
| 5 | Application of third-party mediation for medical disputes: an introduction of Chinese experience显示文摘 | Cao Yanlin Wang Jiangjun Zheng Xueqian Jin Qinghan Tian Yongquan Wen Xuebin Wei Zhanying Li Wu | 2014 | Chinese Medical Journal2014，，14： | 4 |
| 6 | Room-temperature facile synthesis of MnO2on carbon film via UV-photolysis for supercapacitor显示文摘A facile UV-photolysis method has been developed to synthesize birnessite-type MnO2 nanosheets on carbon film at room temperature for supercapacitor electrode materials.The results clearly demonstrated that the specific capacitance of carbon/MnO2 composite electrodes was controlled by the adjustable UV-photolysis reaction time in KMnO4 solution.For the optimized composite electrode obtained after 2 h reaction,a superior specific capacitance of 277 Fg^-1 was achieved at current density of 0.2 Ag^-1.This finding suggests that UV-photolysis is a potential method to fabricate MnO2 on carbon film for supercapacitor. | Shiwei Liu Kai Li Xuebin Zheng | 2019 | Progress in Natural Science:Materials International2019，29，1： | 2 |
| 7 | In vitro antibacterial and osteogenic properties of plasma sprayed silver-containing hydroxyapatite coating显示文摘The objective of the present investigation was to characterize the antibacterial and osteogenic properties of plasma sprayed silver-containing hydroxyapatite (HA/Ag) coating in vitro. HA/Ag coating was deposited via vacuum plasma spraying. The concentration of silver ions released from HA/Ag coating, the efficacy of the HA/Ag coating against bacterial biofilm development, the effect of the HA/Ag coating on early adhesion and ossification of osteoblast cells in vitro was measured. The silver ion concentration released from the HA/Ag coating was between the minimum inhibitory concentration to bacteria and the cytotoxic concentration. Bacterial biofilm inhibition studies indicated an antibacterial activity on the HA/Ag coating surface when compared with hydroxyapatite (HA) coating alone. Moreover, it was demonstrated that osteoblast cell adhesion and mineralization occurred on the HA/Ag coating surface during the testing period. We conclude that the vacuum plasma sprayed HA/Ag coating possesses good antibacterial capability and osteogenic properties in vitro and represents a promising candidate for coating orthopedic implants. | RUAN HongJiang FAN CunYi ZHENG XueBin ZHANG Yan CHEN YiKai | 2009 | Chinese Science Bulletin2009，54，23： | 2 |
| 8 | Effect of microstructure evolution and crystal structure on thermal properties for plasma-sprayed RE_(2)SiO_(5)(RE=Gd,Y,Er)environmental barrier coatings显示文摘In this work,the microstructure evolution,thermal expansion,thermal conductivity,and thermal shock resistance properties of the plasma-sprayed Xl-Gd_(2)SiO_(5),X2-Y_(2)SiO_(5),and X2-Er_(2)SiO_(5)coatings were evaluated and compared by experimental measurement and theoretical exploration.Results showed that significant microstructure evolution such as crystallization of amorphous phase,grain growth,and defects reduction was observed in the RE_(2)SiO_(5)coatings after thermal aging at 1400℃.The Xl-Gd_(2)SiO_(5)coating exhibited higher CTE values than the X2-Y_(2)SiO_(5)and X2-Er_(2)SiO_(5)coatings,which was related to their crystal structure.The thermal conductivity of thermal-aged RE_(2)SiO_(5)coating was much higher than that of the as-sprayed RE_(2)SiO_(5)coating,and thermal conductivity was determined not only by crystal structure but also mainly by the microstructure of the coatings.The X2-Y_(2)SiO_(5)and X2-Er_(2)SiO_(5)coatings with lower thermal mismatch stre s ses presented much better thermal shock resistance than the X1-Gd_(2)SiO_(5)coating. | Xin Zhong Tao Zhu Yaran Niu Haijun Zhou Le Zhang Xiangyu Zhang Qilian Li Xuebin Zheng | 2021 | Journal of Materials Science & Technology2021，，26： | 1 |
| 9 | Bond strength of plasma-sprayed hydroxyapatite/Ti composite coatings显示文摘 | Zheng Xuebin Huang Minhui Ding Chuanxian | 2000 | Biomaterials2000，21，： | 1 |
| 10 | Anti-bacterial and cytotoxic properties of plasma sprayed silver-containing HA coatings显示文摘 | Yikai Chen Xuebin Zheng Youtao Xie Chuanxian Ding Hongjiang Ruan Cunyi Fan | 2008 | Journal of Materials Science: Materials in Medicine2008，，12： | 1 |
| 11 | Plasma-sprayer diopside coatings for biomedical applications显示文摘 | Xue Weichang Liu Xuanyong Zheng Xuebin Ding Chuanxian | 2004 | Surface & Coatings Technology2004，185，23： | 1 |
| 12 | Microstruc- ture characteristics of silicon carbide coatings fabricated on C/C composites by plasma spraying technology显示文摘 | Niu Yaran Zheng Xuebin Ding Chuanxian | 2011 | Ceram Inter2011，37，5： | 1 |
| 13 | Improved stability of plasma-sprayed dicalcium silicate/zirconia composite coating显示文摘 | Xie Youtao Liu Xuanyong Zheng Xuebin Ding Chuanxian Chu Paul K | 2006 | Thin Solid Films2006，515，3： | 1 |
| 14 | Oxidation Behavior of ZrO2 Reinforced MoSi2 Composite Coatings Fabricated by Vacuum Plasma Spraying Technology显示文摘 | Xiaoai Fei Yaran Niu Heng Ji Liping Huang Xuebin Zheng | 2010 | Journal of Thermal Spray Technology2010，，5： | 1 |
| 15 | Microstructure and Properties Characterization of Silicon Coatings Prepared by Vacuum Plasma Spraying Technology显示文摘 | Yaran Niu Xuanyong Liu Xuebin Zheng Heng Ji Chuanxian Ding | 2009 | Journal of Thermal Spray Technology2009，，3： | 1 |
| 16 | LINK PERDURABILITY BASED ROUTING FOR MOBILE AD HOC NETWORKS显示文摘Ad Hoc networks are prone to link failures due to mobility. In this letter, a link perdurability based routing scheme is proposed to try dealing with this problem. This scheme uses signal strength measurements to estimate the route life time and hence chooses a stable route, and is implemented in two typical ad hoc routing protocols to evaluate its performance. The simulation results have shown that this scheme can improve these protocols' packet delivery ratio in cases where there are frequent link failures. | Sun Xuebin Zhou Zheng (Beijing University of Posts and Telecommunications, Beijing 100876) | 2003 | Journal of Electronics(China)2003，20，4： | 1 |
| 17 | High-fluxceramic membranes with a nanomesh of metal oxide nanofibers显示文摘 | KE Xuebin ZHENG Zhenfeng LIU Hongwei | 2008 | J Plays Chem B2008，112，16： | 1 |
| 18 | Bond strength of plasma-sprayed hydroxyapatite/Ti composite coating显示文摘 | Zheng Xuebin Hang Minhui Ding Chuanxian | 2000 | Biomaterials2000，21，8： | 1 |
| 19 | A Novel UWB Pulse Design Based on Chirp Wave form with Muhiband Division显示文摘 | HU Hongsheng SUN Xuebin ZHOU Zheng | 2010 | AIA12010，1049，10： | 1 |
| 20 | Evidence for the role of KIF17 in fish spermatid reshaping:expression pattern of KIF17 in Larimichthys polyactis spermiogenesis显示文摘The homodimeric kinesin-2 protein KIF17 functions in intracellular transport and spermiogenesis in mammals.However,its role in fish spermiogenesis has not been reported.Here,we aimed to clone full-length kif17 cDNA and determine the molecular characteristics and expression patterns of KIF17 in Larimichthys polyactis spermiogenesis.The full-length cDNA of L.polyactis kif17(Lp-kif17)was sequenced and found to contain a 332-bp 5′untranslated region,480-bp 3′untranslated region,and 2433-bp open reading frame encoding 810 amino acids.Bioinformatics analyses showed that L.polyactis KIF17(Lp-KIF17)shared high sequence similarity with homologs in other animals and possessed an N-terminal motor domain with microtubule-binding sites and adenosine triphosphate(ATP)hydrolysis sites,a stalk domain containing two coiled-coil regions,and a C-terminal tail domain.The Lp-kif17 mRNA was widely expressed in various tissues,with the highest level in the brain,followed by that in the testis.Fluorescence in situ hybridization(FISH)analysis revealed that Lp-kif17 was continuously expressed in spermiogenesis,showing that it had potential functions in this process.Using immunofluorescence(IF)analysis,we found that Lp-KIF17 colocalized with tubulin and was transferred from the perinuclear cytoplasm to the side of spermatid where the tail forms during spermiogenesis.These findings suggested that KIF17 is involved in L.polyactis spermiogenesis.In particular,it may participate in nuclear shaping and tail formation by interacting with perinuclear microtubules during spermatid reshaping.In addition to providing evidence for the role of KIF17 in fish spermatid reshaping,this study provides important data for studies of reproductive biology in L.polyactis. | Jingqian WANG Xinming GAO Xuebin ZHENG Chen DU Congcong HOU Qingping XIE Bao LOU Feng LIU Shan JIN Junquan ZHU | 2021 | Journal of Oceanology and Limnology2021，39，6： | 1 |
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