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| 1 | KAI1/CD82 suppresses hepatocyte growth factorinduced migration of hepatoma cells via upregulationof Sprouty2显示文摘We conducted a study concerning the suppressive mechanism of KAI1/CD82 on hepatoma cell metastasis.Hepatocyte growth factor(HGF)induces the migration of hepatoma cells through activation of cellular sphingosine kinase 1(SphK1).Adenovirus-mediated gene transfer of KAI1(Ad-KAI1)downregulates the SphK1 expression and suppresses the HGF-induced migration of SMMC-7721 human hepatocellcular carcinoma cells.Overexpression of KAI1/CD82 significantly elevates Sprouty2 at the protein level.Ablation of Sprouty2 with RNA interference can block the KAI1/CD82-induced suppression of hepatoma cell migration and downregulation of SphK1 expression.It is demonstrated that KAI1/CD82 suppresses HGF-induced migration of hepatoma cells via upregulation of Sprouty2. | MU ZhenBin1 ,3,WANG Hua 2,ZHANG Jing 2,LI QingFang 2,WANG LiSheng 2&GUO XiaoZhong 3 1State Key Laboratory of Cancer Biology and Institute of Digestive Diseases,Xijing Hospital,Fourth Military Medical University,Xi’an 710032,China 2Department of Experimental Hematology,Beijing Institute of Radiation Medicine,Beijing 100850,China 3Department of Gastroenterology,Shenyang General Hospital of PLA,Shenyang 110016,China | 2008 | Science China(Life Sciences)2008，51，7： | 7 |
| 2 | Whole-genome sequencing provides insights into the genetic diversity and domestication of bitter gourd(Momordica spp.)显示文摘Bitter gourd(Momordica charantia)is a popular cultivated vegetable in Asian and African countries.To reveal the characteristics of the genomic structure,evolutionary trajectory,and genetic basis underlying the domestication of bitter gourd,we performed whole-genome sequencing of the cultivar Dali-11 and the wild small-fruited line TR and resequencing of 187 bitter gourd germplasms from 16 countries.The major gene clusters(Bi clusters)for the biosynthesis of cucurbitane triterpenoids,which confer a bitter taste,are highly conserved in cucumber,melon,and watermelon.Comparative analysis among cucurbit genomes revealed that the Bi cluster involved in cucurbitane triterpenoid biosynthesis is absent in bitter gourd.Phylogenetic analysis revealed that the TR group,including 21 bitter gourd germplasms,may belong to a new species or subspecies independent from M.charantia.Furthermore,we found that the remaining 166 M.charantia germplasms are geographically differentiated,and we identified 710,412,and 290 candidate domestication genes in the South Asia,Southeast Asia,and China populations,respectively.This study provides new insights into bitter gourd genetic diversity and domestication and will facilitate the future genomics-enabled improvement of bitter gourd. | Junjie Cui Yan Yang Shaobo Luo Le Wang Rukui Huang Qingfang Wen Xiaoxia Han Nansheng Miao Jiaowen Cheng Ziji Liu Changyuan Zhang Chengcheng Feng Haisheng Zhu Jianwen Su Xinjian Wan Fang Hu Yu Niu Xiaoming Zheng Yulan Yang Dai Shan Zhensheng Dong Weiming He Narinder P.S.Dhillon Kailin Hu | 2020 | Horticulture Research2020，7，1： | 7 |
| 3 | Antitumor effects of an engineered and energized fusion protein consisting of an anti-CD20 scFv fragment and lidamycin显示文摘Antibody-based fusion proteins are the next generation of antibody therapies for cancer and other diseases.CD20 antigen,which is overexpressed on cell membranes in nearly 95% of cases of B-cell Non-Hodgkin's Lymphoma,is an attractive target for the therapy of B-lymphoid malignancies.Lidamycin (LDM) is a potent enediyne-containing antitumor antibiotic that now has entered phase II clinical trials.In this study,we prepared an engineered fusion protein,scFv-LDP,consisting of an anti-CD20 scFv fragment and the apoprotein LDP of LDM using DNA recombination.After purification and refolding,scFv-LDP was found to bind specifically to CD20-positive lymphoma cells using ELISA and indirect immunofluorescent cytochemical staining assays.The energized fusion protein scFv-LDP-AE was obtained using molecular reconstitution of the active chromophore AE of LDM and scFv-LDP.MTT assay revealed potent cytotoxicity of scFv-LDP-AE to CD20-positive Raji and Daudi cells,with IC 50 values of 1.21×10-11 and 6.24×10-11 mol L-1,respectively.An in vivo subcutaneous xenograft model of CD20-positive B cell lymphoma in BALB/c (nu/nu) mice was also utilized.Drugs were given intravenously on day 14 and 21 after tumor transplantation.In terms of maximal tolerated doses,scFv-LDP-AE at 0.3 mg kg-1 suppressed tumor growth by 79.3%,and LDM at 0.05 mg kg-1 by 68.6% (P<0.05).Results suggested scFv-LDP-AE could be a potential candidate for tumor-targeting therapy. | FANG Hong MIAO QingFang ZHANG ShengHua CHENG Xin XIONG DongSheng ZHEN YongSu | 2011 | Science China(Life Sciences)2011，54，3： | 6 |
| 4 | Establishment and evaluation of a peanut association panel and analysis of key nutritional traits显示文摘Breeding programs aim to improve the yield and quality of peanut(Arachis hypogaea L.); using association mapping to identify genetic markers linked to these quantitative traits could facilitate selection efficiency. A peanut association panel was established consisting of 268 lines with extensive phenotypic and genetic variation, meeting the requirements for association analysis. These lines were grown over 3 years and the key agronomic traits, including protein and oil content were examined. Population structure(Q) analysis showed two subpopulations and clustering analysis was consistent with Q-based membership assignment and closely related to botanical type. Relative Kinship(K) indicated that most of the panel members have no or weak familial relatedness, with 52.78% of lines showing K ? 0. Linkagedisequilibrium(LD) analysis showed a high level of LD occurs in the panel. Model comparisons indicated false positives can be effectively controlled by taking Q and K into consideration and more false positives were generated by K than Q. A preliminary association analysis using a Q t K model found markers significantly associated with oil, protein, oleic acid, and linoleic acid, and identified a set of alleles with positive and negative effects. These results show that this panel is suitable for association analysis,providing a resource for marker-assisted selection for peanut improvement. | Xiurong Zhang Suqing Zhu Kun Zhang Yongshan Wan Fengzhen Liu Qingfang Sun Yingjie Li | 2018 | Journal of Integrative Plant Biology2018，60，3： | 5 |
| 5 | The heterogeneity of islet autoantibodies and the progression of islet failure in type 1 diabetic patients显示文摘Type 1 diabetes mellitus is heterogeneous in many facets. The patients suffered from type 1 diabetes present several levels of islet function as well as variable number and type of islet-specific autoantibodies. This study was to investigate prevalence and heterogeneity of the islet autoantibodies and clinical phenotypes of type 1 diabetes mellitus; and also discussed the process of islet failure and its risk factors in Chinese type 1 diabetic patients. A total of 1,291 type 1 diabetic patients were enrolled in this study. Demographic information was collected. Laboratory tests including mixed-meal tolerance test, human leukocyte antigen alleles, hemoglobin A1 c, lipids, thyroid function and islet autoantibodies were conducted. The frequency of islet-specific autoantibody in newly diagnosed T1 DM patients(duration shorter than half year) was 73% in East China. According to binary logistic regressions, autoantibody positivity, longer duration and lower Body Mass Index were the risk factors of islet failure. As the disease developed, autoantibodies against glutamic acid decarboxylase declined as well as the other two autoantibodies against zinc transporter 8 and islet antigen 2. The decrease of autoantibodies was positively correlated with aggressive beta cell destruction. Autoantibodies can facilitate the identification of classic T1 DM from other subtypes and predict the progression of islet failure. As there were obvious heterogeneity in autoantibodies and clinical manifestation in different phenotypes of the disease, we should take more factors into consideration when identifying type 1 diabetes mellitus. | Jin Liu Lingling Bian Li Ji Yang Chen Heng Chen Yong Gu Bingqin Ma Wei Gu Xinyu Xu Yun Shi Jian Wang Dalong Zhu Zilin Sun Jianhua Ma Hui Jin Xing Shi Heng Miao Bing Xin Yan Zhu Zhenwen Zhang Ruifang Bu Lan Xu Guangde Shi Wei Tang Wei Li Dongmei Zhou Jun Liang Xingbo Cheng Bimin Shi Jixiang Dong Ji Hu Chen Fang Shao Zhong Weinan Yu Weiping Lu Chenguang Wu Li Qian Jiancheng Yu Jialin Gao Xiaoqiang Fei Qingqing Zhang Xueqin Wang Shiwei Cui Jinluo Cheng Ning Xu Guofeng Wang Guoqing Han Chunrong Xu Yun Xie Minmin An Wei Zhang Zhixiao Wang Yun Cai Qi Fu Yu Fu Shuai Zheng Fan Yang Qingfang Hu Hao Dai Yu Jin Zheng Zhang Kuanfeng Xu Yifan Li Jie Shen Hongwen Zhou Wei He Xuqin Zheng Xiao Han Liping Yu Jinxiong She Mei Zhang Tao Yang | 2016 | Science China(Life Sciences)2016，59，9： | 5 |
| 6 | Regulatory effect of salicylic acid and methyl jasmonate supplementation on ergosterol production in Hericium erinaceus mycelia显示文摘We evaluated the biomass and ergosterol content of Hericium erinaceus mycelium, and extracellular enzyme activities in H. erinaceus liquid culture following salicylic acid(SA) and methyl jasmonic acid(Me JA)supplementation. The optimal SA concentration was100 lmoláL-1, where the highest ergosterol content of 2.33 mgág-1was obtained following 6-day cultivation with100 lmoláL-1SA supplementation, and which was significantly higher than the unsupplemented control(p \ 0.01). Following 4-day supplementation with50 lmoláL-1Me JA, the highest ergosterol content obtained was 1.988 mgág-1, which was 25.8 % higher than the unsupplemented control. Our data indicate that SA and Me JA supplementation improves ergosterol content in H.erinaceus mycelium. | Xiaodong Dai Yaguang Zhan Jiechi Zhang Piqi Zhang Zenghua Han Qingfang Ma Xianghui Kong Jianing Liu Yinpeng Ma | 2015 | Journal of Forestry Research2015，26，1： | 5 |
| 7 | Crystalline behaviors and phase transition during the manufacture of fine denier PA6 fibers显示文摘Recently we have successfully produced fine denier PA6 fibers by using additives containing lanthanide compounds.Meanwhile,crystallization and phase transition of PA6 fibers during spinning and drawing processes were investigated.During the spinning process,β phase crystal could be obtained in as-spun PA6 fibers which were produced with relatively high melt draw ratio,while γ phase crystal predominated when the melt draw ratio was relatively low.β phase crystal,whose behaviors are similar with those of γ phase by FT-IR and XRD characterization,could be transformed to α form easily when PA6 fibers are immersed in boiling water.However,γ phase crystal of PA6 remains unchanged in boiling water.Thus,β and γ phase crystals of PA6 can be differentiated by the crystalline behaviors of PA6 fibers after treatment in boiling water.Further experiments demonstrate that the β phase can also be produced during a drawing process where a phase transformation from γ to α occurs.In other words,β phase may act as an intermediate state during the phase transformation. | ZHANG ChengFeng LIU YuHai LIU ShaoXuan LI HuiZhen HUANG Kun PAN QingHua HUA XiaoHui HAO ChaoWei MA QingFang LV ChangYou LI WeiHong YANG ZhanLan ZHAO Ying WANG DuJin LAI GuoQiao JIANG JianXiong XU YiZhuang WU JinGuang | 2009 | Science China Chemistry2009，52，11： | 4 |
| 8 | Kinetic study of the hydrocarbon generation from marine carbonate source rocks characterization of products of gas and liquid hydrocarbon显示文摘The kinetic parameters of hydrocarbon generation from the marine carbonate source rocks were determined and calibrated through kinetic simulating experiment. The kinetic parameters of hydrocarbon generation then were extrapolated to geological condition by using the relative software. The result shows that gaseous hydrocarbons (C_1, C_2, C_3, C_(4-5)) were generated in condition of 150℃ | GENG Xinhua GENG Ansong XIONG Yongqiang LIU Jinzhong ZHANG Haizu ZHAO Qingfang | 2006 | Chinese Science Bulletin2006，51，23： | 3 |
| 9 | Effect of Soil Drying Intensity During an Experimental Drying-Rewetting Event on Nutrient Transformation and Microbial Community Composition显示文摘Soil drying-rewetting(DRW) events affect nutrient transformation and microbial community composition; however, little is known about the influence of drying intensity during the DRW events. Therefore, we analyzed soil nutrient composition and microbial communities with exposure to various drying intensities during an experimental drying-rewetting event, using a silt loam from a grassland of northern China, where the semi-arid climate exposes soils to a wide range of moisture conditions, and grasslands account for over 40% of the nation's land area. We also conducted a sterilization experiment to examine the contribution of soil microbes to nutrient pulses. Soil drying-rewetting decreased carbon(C) mineralization by 9%–27%. Both monosaccharide and mineral nitrogen(N) contents increased with higher drying intensities(drying to ≤ 10% gravimetric water content), with the increases being 204% and 110% with the highest drying intensity(drying to 2% gravimetric water content), respectively, whereas labile phosphorus(P)only increased(by 105%) with the highest drying intensity. Moreover, levels of microbial biomass C and N and dissolved organic N decreased with increasing drying intensity and were correlated with increases in dissolved organic C and mineral N, respectively,whereas the increases in labile P were not consistent with reductions in microbial biomass P. The sterilization experiment results indicated that microbes were primarily responsible for the C and N pulses, whereas non-microbial factors were the main contributors to the labile P pulses. Phospholipid fatty acid analysis indicated that soil microbes were highly resistant to drying-rewetting events and that drought-resistant groups were probably responsible for nutrient transformation. Therefore, the present study demonstrated that moderate soil drying during drying-rewetting events could improve the mineralization of N, but not P, and that different mechanisms were responsible for the C, N, and P pulses observed during drying-rewetting events. | SUN Dashend BI Qingfang LI Kejie ZHU Jun ZHANG Qichun JIN Chongwei LU Lingli LIN Xianyong | 2018 | Pedosphere2018，28，4： | 2 |
| 10 | Energy consumption and GHG emissions of six biofuel pathways by LCA in (the) People’s Republic of China显示文摘 | Xunmin Ou Xiliang Zhang Shiyan Chang Qingfang Guo | 2009 | Applied Energy2009，，： | 1 |
| 11 | Synthesis and properties of poly (urethane-imide) diacid/epoxy composites cured with an aziridine system显示文摘 | Liu Pinggui Zhang Qingfang He Lihua | 2009 | Journal of Applied Polymer Science2009，113，4： | 1 |
| 12 | A hybrid approach for the vehicle routing problem with three-dimensional loading constraints 显示文摘 | Ruan Qingfang Zhang Zhengqian Miao Lixin | 2011 | Computers & Operations Research2011，38，11： | 1 |
| 13 | A Combinatorial Condition and Boolean Functions with Optimal Algebraic Immunity显示文摘This paper first proposes an infinite class of 2k-variable Boolean functions with high nonlinearity and high algebraic degree. Then an infinite class of balanced Boolean functions are proposed by modifying the above Boolean functions. This class of balanced Boolean functions have optimal algebraic degree and high nonlinearity. Both classes have optimal algebraic immunity based on a general combinatorial conjecture. | JIN Qingfang LIU Zhuojun WU Baofeng ZHANG Xiaoming | 2015 | Journal of Systems Science & Complexity2015，28，3： | 1 |
| 14 | Bilateral inferior petrosal sinus sampling for the treatment of Cushing's disease Data from 52 cases from one institute over an eight-year period显示文摘The present study analyzed data from 108 Cushing's disease patients,who underwent trans-sphenoidal surgery in one hospital between January 2003 and April 2010,to investigate the performance value of bilateral inferior petrosal sinus sampling (BIPSS).Of the 108 patients,52 underwent BIPSS prior to surgery and 56 did not.Results showed that BIPSS accuracy for the diagnosis of Cushing's disease was 86.5% (45/52),and accuracy for adenoma lateralization during surgery was 76.9% (40/52).In addition,early remission rate was significantly greater compared to patients without BIPSS.Results demonstrated that BIPSS facilitated localization and diagnosis of Cushing's disease and helped to predict adenoma lateralization. | Changyan Fan Xiuhua Shi Qingfang Sun Liuguan Bian Weiguo Zhao Jiankang Shen Hua Zhang Tingwei Su Weiqing Wang Xiaoying Li Guang Ning Liang Kong Lingling Hu | 2011 | Neural Regeneration Research2011，6，28： | 1 |
| 15 | Study on Crustal Structure in Haiyuan Strong Earthquake Region显示文摘The crust/upper-mantle velocity structure and deep tectonic features are obtained in Haiyuan M = 8. 5 earthquake region from data processing of Xiji-Zhongwei deep seismic sounding (DSS) profile. The result shows that there is a great difference between the velocity structures on the two sides of the epicenter. The crust consists of alternative high and low velocity layers on the southern side while on the northern side it consists of a series of layers with positive velocity gradient. There exists a deep fault cutting Moho or an anomalous deep structural zone in the epicentral region. | Li Songlin,Zhang Xiankang,Zhang Chengke,Ren Qingfang,Shi Jinhu,Zhao Jinren,Fang Shengming,Liu Baofeng,Pan Suzhen, and Zhang Jianshi Research Center of Exploration Geophysics, CSB, Zhengzhou 450002 , China | 2001 | Earthquake Research in China2001，15，3： | 1 |
| 16 | A hybrid approach for the vehicle routing problem with three-dimensional loading constraints显示文摘 | Qingfang Ruan Zhengqian Zhang Lixin Miao Haitao Shen | 2011 | Computers and Operations Research2011，，： | 1 |
| 17 | Exploration and Study on Deep Crust Structural Characteristics in the Jiashi-Artux Seismic Region显示文摘The data from two deep seismic sounding profiles was processed and studied comprehensively. The results show that crust_mantle structures in the investigated region obviously display layered characteristics and velocity structures and tectonic features have larger distinction in different geological structure blocks. The boundary interface C between the upper and lower crust and Moho fluctuate greatly. The shallowest depths of C (30 0km) and Moho (45 5km) under Jiashi deepen sharply from Jiashi to the western Kunlun mountain areas, where the depths of C and Moho are 44 0km and 70 0km, respectively. The higher velocity structures in the Tarim massif determine its relatively “stable” characteristics in crust tectonics. The phenomenon in the Jiashi region, where the distribution of earthquake foci mostly range from 20km to 40km in depth, may infer that the local uplift of C and Moho interface, anomalously lower velocity bodies and deep large faults control earthquake occurrence and seismogenic processes in the Jiashi strong earthquake swarm. | Zhao Jinren, Zhang Xiankang, Zhang Chengke, Ren Qingfang, Cheng Shuangxi, Zhang Jianshi, Nie Wenying anf Pan ShuzhenResearch Center of Exploration Geophysics, China Seismological Bureau, Zhengzhou 450002, China | 2003 | Earthquake Research in China2003，17，2： | 1 |
| 18 | A hybridapproach for the vehicle routing problem with three-dimensionalloading constraints显示文摘 | Qingfang Ruan Zhengqian Zhang LixinMiao etal | 2013 | Computers & Operations Research Volume 40 Issue 62013，2013，： | 1 |
| 19 | Ammonia Volatilization from Soils Fertilized with Different Nitrogen Type and Application Method in Germination and Early Seedling Stages from the Radish Field显示文摘Ammonia volatilization(AV) from basal fertilizer with different nitrogen(N) types and application methods was investigated by the ventilation method in germination and early seedling stages during radish growth seasons in 2014. Four N fertilizer types, urea(U), ammonium bicarbonate(AB), ammonia sulfate(AS), and controlled urea formaldehyde(CUF) were applied through 5 cm depth placement(I) and 10 cm depth placement(II). The results showed that the N fertilizer type was the main factor that caused AV loss in germination and early seedling stages from the radish field. The highest and the lowest cumulative AV losses in germination and early seedling stages from the radish fields were 33.23 and 11.21 N kg/hm^2 for the treatments of AB+I and CUF+II, respectively, accounting for 60.40 and 26.40% of the N application for each treatment. The 10 cm deep placement of N reduced AV rates and lagged the AV process, and CUF significantly reduced ammonia volatilization. The data showed that the suitable N fertilizer type and application method for basal fertilizer were CUF and deep placement, respectively.Therefore, fertilizing with proper N fertilizer types and methods should be the efficient measures to mitigate AV losses from the radish field and will alleviate environment problems. | Weiling YUAN Shangyong YUAN Feng ZHANG Xiaohui DENG Caixia GAN Lei CUI Qingfang WANG | 2016 | Agricultural Science & Technology2016，17，4： | 1 |
| 20 | Enhancement of terbium efficiency by gallium and copper co-doping in(Pr,Nd)-Fe-B sintered magnets显示文摘It is well known that Tb substitution for(Pr,Nd)in(Pr,Nd)-Fe-B based sintered magnetic materials is an effective way to increase intrinsic coercivity,but it is not quite clear whether the increment depends on the different matrix phases with various doping ingredient or not,which is essential to develop high quality magnets with high coercivity more efficiently and effectively with economic consumption of expensive Tb and other costly heavy rare earths.In this paper,we investigated the efficiency of Tb substitution for magnetic property in(Pr,Nd)-Fe-B sintered permanent magnets by co-doping Ga and Cu elements.It is shown that Ga and Cu co-doping can effectively improve the efficiency of Tb substitution to increase the thermal stability and the coercivity.The intrinsic coercivity increases up to 549 and 987 kA/m respectively by 1.5 wt%and 3.0 wt%Tb substitution in Ga and Cu co-doped magnets while the intrinsic coercivity increases up to only 334 and 613 kA/m respectively by the same amounts of Tb substitution in non-Ga and low-Cu magnets.In other words,it demonstrates that there is about 329-366 kA/m linear equivalent enhancement of intrinsic coercivity by 1.0 wt%Tb substitution for(Pr,Nd)in Ga and Cu co-doped magnets.The temperature coefficients of both intrinsic coercivityβand remanenceαat 20-150℃by 3.0 wt%Tb substitution for the magnets with Ga and Cu co-doping are-0.47%/K and-0.109%/K respectively,and in contrast those values are-0.52%/K and 0.116%/K respectively for the non-Ga and low-Cu magnets.It is the principal reason for more efficient enhancement of magnetic property by Tb substitution in the Ga and Cu co-doped magnets in which Tb atoms are expelled from triple junction phases(TJPs)to penetrate into the grain boundary phases(GB phases)and thus modify the grain boundary.It is prospected that the efficiency of Tb substitution would rely on different matrix phases with various doping constituents. | Qingfang Huang Qingzheng Jiang Yao Shi Sajjad Ur Rehman Xing Wei Ruoshu Wang Yongjian Zhang Deqin Xu Dakun Chen Zhenchen Zhong | 2023 | Journal of Rare Earths2023，41，4： | 0 |
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