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| 1 | Natural variation of GmFATA1B regulates seed oil content and composition in soybean显示文摘Soybean(Glycine max) produces seeds that are rich in unsaturated fatty acids and is an important oilseed crop worldwide. Seed oil content and composition largely determine the economic value of soybean. Due to natural genetic variation, seed oil content varies substantially across soybean cultivars. Although much progress has been made in elucidating the genetic trajectory underlying fatty acid metabolism and oil biosynthesis in plants, the causal genes for many quantitative trait loci(QTLs) regulating seed oil content in soybean remain to be revealed. In this study, we identified Gm FATA1B as the gene underlying a QTL that regulates seed oil content and composition, as well as seed size in soybean. Nine extra amino acids in the conserved region of Gm FATA1B impair its function as a fatty acyl–acyl carrier protein thioesterase, thereby affecting seed oil content and composition. Heterogeneously overexpressing the functional Gm FATA1B allele in Arabidopsis thaliana increased both the total oil content and the oleic acid and linoleic acid contents of seeds. Our findings uncover a previously unknown locus underlying variation in seed oil content in soybean and lay the foundation for improving seed oil content and composition in soybean. | Zhandong Cai Peiqi Xian Yanbo Cheng Yuan Yang Yakun Zhang Zihang He Chuwen Xiong Zhibin Guo Zhicheng Chen Huiqian Jiang Qibin Ma Hai Nian Liangfa Ge | 2023 | Journal of Integrative Plant Biology2023，65，10： | 0 |
| 2 | Efficient Hierarchical Multi-Server Authentication Protocol for Mobile Cloud Computing显示文摘With the development of communication technologies,various mobile devices and different types of mobile services became available.The emergence of these services has brought great convenience to our lives.The multi-server architecture authentication protocols for mobile cloud computing were proposed to ensure the security and availability between mobile devices and mobile services.However,most of the protocols did not consider the case of hierarchical authentication.In the existing protocol,when a mobile user once registered at the registration center,he/she can successfully authenticate with all mobile service providers that are registered at the registration center,but real application scenarios are not like this.For some specific scenarios,some mobile service providers want to provide service only for particular users.For this reason,we propose a new hierarchical multi-server authentication protocol for mobile cloud computing.The proposed protocol ensures only particular types of users can successfully authenticate with certain types of mobile service providers.The proposed protocol reduces computing and communication costs by up to 42.6%and 54.2%compared to two superior protocols.The proposed protocol can also resist the attacks known so far. | Jiangheng Kou Mingxing He Ling Xiong Zihang Ge Guangmin Xie | 2020 | Computers, Materials & Continua2020，，7： | 0 |
| 3 | SETD2 mediates immunotherapy and radiotherapy efficacy via regulating DNA damage responses and genomic stability in lung adenocarcinoma显示文摘SETD2 is the main transferase for the trimethylation of histone H3 at lysine 36 (H3K36me3) in mammals. SETD2 plays important role in repairing DNA double strand breaks and maintaining chromatin integrity.1 In renal carcinoma, SETD2 deficiency caused DNA replication fork instability and DNA damage.2 The absence of SETD2 was also reported to have strong tumor-promoting effects in lung adenocarcinomas (LUAD).3 However, the other roles of SETD2 remain poorly understood in LUAD. In this study, we utilized comprehensive omics-data analysis to determine that SETD2 was associated with DNA damage and immune-related signals in LUAD. SETD2 knockdown induced DNA damage and cGAS activation in LUAD cells, and reduced the number of cells at the G1 phase. Moreover, SETD2 deficiency was conducive to mutation burden, immune cell infiltration, and immunotherapy responses. High SETD2 expression was associated with high radiocurability. These findings suggest that SETD2 may be a promising biomarker of therapeutic responses for LUAD patients, and offer novel insights into immunotherapy and radiotherapy. | Zihang Zeng Yanping Gao Jiali Li Jianguo Zhang Yangyi Li Fajian He Zhengrong Huang Linzhi Han Yan Gong Conghua Xie | 2023 | Genes & Diseases2023，10，2： | 0 |
| 4 | A method for determining the cutting efficiency of the CRISPR/Cas system in birch and poplar显示文摘Determination of Cas9 cutting efficiency to the target sites is important for genome editing.However,this determination can only be made via an in vitro method,as the purification of Cas protein and synthesis of gRNA are necessary.Here,we developed an in vivo method,called transient CRISPR/Cas editing in plants(TCEP)to determine Cas9 cutting efficiency.The CRISPR/Cas vector for plant transformation mediated by Agrobacterium tumefaciens was constructed as normal.Using the transient transformation method we built,the Cas9 protein and gRNA were transiently expressed and formed a complex to cut its target sites,resulting in dynamic DNA breakage.The broken DNA was quantified using qPCR to measure the efficiencies of Cas9 cutting.We studied the Cas9 cutting efficiencies to different target sites in Betula platyphylla and Populus davidiana×P.bolleana plants using TCEP and an in vitro method.The results of TCEP were consistent with those of the in vitro method,suggesting that the TCEP method is reliable in determining cutting efficiency.Additionally,using the TCEP method,we showed that both heat and sonication treatment significantly improved CRISPR/Cas efficiency.Therefore,the TCEP method has broad application value and can not only be used to analyze the CRISPR/Cas efficiency but also to determine the factors involved in Cas9 cutting. | Zhibo Wang Zihang He Ming Qu Zhujun Liu Chao Wang Yucheng Wang | 2021 | Forestry Research2021，1，1： | 0 |
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