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		题名	作者	年代	出处	被引量
	1	In vitro effects of polyphenols on colorectal cancer cells显示文摘AIM:To investigate the effects of quercetin and genistein on colon cancer cell proliferation and their estrogen receptorβ(ERβ)expression.METHODS:Colon cancer cells were stably transfected with a mammalian expression vector to overexpress ERβ(HCT8-β8-expressing cells)or a control vector(HCT8-pSV2neo-expressing cells).The proliferation of these cells was examined after treatment with quercetin or genistein(5-100μmol/L),or 10 nmol/L17β-estradiol(17β-E2).Cell viability was examined by acridine orange staining following treatments for 48 or144 h.Effects of quercetin and genistein on ERβtranscriptional transactivation were examined by luciferase activity in HCT8-β8-expressing cells transiently transfected with a pEREtkLUC reporter vector.In addition,the regulation of ERβtranscription by phytoestrogens and 17β-E2 was examined by quantitative polymerase chain reaction.RESULTS:Proliferation of HCT8-β8-expressing cells was not reduced low doses(5μmol/L)of quercetin and genistein,while it was reduced at 25-50μmol/L with an effect similar to 10 nmol/L 17β-E2.Treatment with doses of phytoestrogens≥75μmol/L completely blocked cell growth and reduced overall cell counts,however no effects at any dose were observed in HCT8-pSV2neoexpressing cells.These results were supported by viability staining that revealed acridine orange-stained lysosomes with high doses or extended treatment periods.Genistein and quercetin(50μmol/L)significantly increased ER-responsive luciferase activity similar to 10nmol/L 17β-E2(P<0.05).Furthermore,genistein and quercetin(50μmol/L),as well as 10 nmol/L 17β-E2significantly increased ERβmRNA levels in HCT8-β8-expressing cells(P<0.05).In addition,treatment of HCT8-pSV2neo-expressing cells with 50μmol/L quercetin or 10 nmol/L 17β-E2 significantly increased ERβmRNA levels compared to untreated controls(P<0.05),though the absolute levels were much lower than in HCT8-β8-expressing cells.CONCLUSION:The antitumorigenic effects of the phytoestrogenic compounds quercetin and genistein on colon cancers cells occur through ERβactivity and expression.	Barbara Pampaloni Gaia Palmini Carmelo Mavilia Roberto Zonefrati Annalisa Tanini Maria Luisa Brand	2014	World Journal of Gastrointestinal Oncology2014，6，8：	2
	2	Neuroblast long stem cell culture from human fetal olfactory epithelium respond to odors 显示文摘	Vannelli GB Ensoli F Zonefrati F	1995	J Neurosci1995，15，6：	1
	3	In vitro differentiation of human mesenchymal stem cells on Ti6Al4V surfaces显示文摘	Tognarini I Sorace S Zonefrati R	2008	Biomaterials2008，29，7：	1
	4	In vitro differentiation of hu- man mesenchymal stem cells on Ti6A14V surfaces显示文摘	Tognarini I Sorace S Zonefrati R	2008	Biomaterials2008，29，7：	1
	5	Sputum processing:a new method to improve cytospin quality显示文摘	Ronchi MC Galli G Zonefrati R		0，，：	1
	6	Osteodifferentiation of Human Preadipocytes Induced by Strontium Released from Hydrogels显示文摘	Valeria Nardone Sergio Fabbri Francesca Marini Roberto Zonefrati Gianna Galli Annamaria Carossino Annalisa Tanini Maria Luisa Brandi Giovanni Vozzi	2012	International Journal of Biomaterials2012，，：	1
	7	In vitro differentiation of human mesenchymal stem cells on Ti6Al4V surfaces显示文摘	Isabella Tognarini Sabina Sorace Roberto Zonefrati Gianna Galli Alessia Gozzini Silvia Carbonell Sala Giorgia D. Zappoli Thyrion Anna Maria Carossino Annalisa Tanini Carmelo Mavilia Chiara Azzari Fausto Sbaiz Alessandro Facchini Rodolfo Capanna Maria Luis	2007	Biomaterials2007，，7：	1

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