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| 1 | A transceptor-channel complex couples nitrate sensing to calcium signaling in Ambidopsis显示文摘Nitrate-induced Ca^(2+) signaling is crucial for the primary nitrate response in plants.However,the molecular mechanism underlying the generation of the nitrate-specific calcium signature remains unknown.We report here that a cyclic nucleotide-gated channel(CNGC)protein,CNGC15,and the nitrate transceptor(NRT1.1)constitute a molecular switch that controls calcium influx depending on nitrate levels.The expression of CNGC15 is induced by nitrate,and its protein is localized at the plasma membrane after establishment of young seedlings.We found that disruption of CNGC15 results in the loss of the nitrate-induced Ca^(2+) signature(primary nitrate response)and retards root growth,reminiscent of the phenotype observed in the nrt1.1 mutant.We further showed that CNGC15 is an active Ca^(2+)-permeable channel that physically interacts with the NRT1.1 protein in the plasma membrane.Importantly,we discovered that CNGC15-NRT1.1 interaction silences the channel activity of the heterocomplex,which dissociates upon a rise in nitrate levels,leading to reactivation of the CNGC15 channel.The dynamic interactions between CNGC15 and NRT1.1 therefore control the channel activity and Ca^(2+) influx in a nitrate-dependent manner.Our study reveals a new nutrient-sensing mechanism that utilizes a nutrient transceptor-channel complex assembly to couple nutrient status to a specific Ca^(2+) signature. | Xiaohan Wang Changxin Feng LiLi Tian Congcong Hou Wang Tian Bin Hu Qian Zhang Zhijie Ren Qi Niu Jiali Song Dongdong Kong Liangyu Liu Yikun He Ligeng Ma Chengcai Chu Sheng Luan Legong Li | 2021 | Molecular Plant2021，14，5： | 5 |
| 2 | The interaction of CaM7 and CNGC14 regulates root hair growth in Arabidopsis显示文摘Oscillations in cytosolic free calcium determine the polarity of tip-growing root hairs.The Ca2+channel cyclic nucleotide gated channel 14(CNGC14)contributes to the dynamic changes in Ca^2+concentration gradient at the root hair tip.However,the mechanisms that regulate CNGC14 are unknown.In this study,we detected a direct interaction between calmodulin 7(CaM7)and CNGC14 through yeast two-hybrid and bimolecular fluorescence complementation assays.We demonstrated that the third EF-hand domain of CaM7 specifically interacts with the cytosolic C-terminal domain of CNGC14.A two-electrode voltage clamp assay showed that CaM7 completely inhibitsCNGC14-mediated Ca2+influx,suggesting that CaM7 neg-atively regulates CNGC14-mediated calcium signaling.Fur-thermore,CaM7 overexpressing lines phenocopy the short root hair phenotype of a cngc14 mutant and this phenotype is insensitive to changes in external Ca^2+concentrations.We,thus,identified CaM7-CNGC14 as a novel interacting module that regulates polar growth in root hairs by con-trolling the tip-focused Ca2+signal. | Qudsia Zeb Xiaohan Wang Congcong Hou Xiwen Zhang Mengqi Dong Sisi Zhang Qian Zhang Zhijie Ren Wang Tian Huifen Zhu Legong Li Liangyu Liu | 2020 | Journal of Integrative Plant Biology2020，62，7： | 3 |
| 3 | 霸王环核苷酸门控通道基因ZxCNGC5的克隆及表达模式分析显示文摘钙离子(Ca^(2+))作为生命活动过程中重要的第二信使,对植物的生长发育至关重要。环核苷酸门控离子通道(cyclic nucleotide-gated channels,CNGCs)是一类Ca^(2+)通道,在植物的花粉发育、免疫反应以及响应逆境胁迫等生理过程中发挥着重要作用。本研究通过RACE和RT-PCR技术克隆了荒漠旱生植物霸王(Zygophyllum xanthoxylum)CNGCs家族的一个编码基因,命名为ZxCNGC5。生物信息学分析显示其编码728个氨基酸,包含CNGCs家族所特有的6个跨膜域、1个P环以及环核苷酸结合域(cyclic nucleotide-binding domain,CNBD)和钙调蛋白结合域(calmodulin-binding domain,CaMBD);系统进化树将CNGCs家族分为5个亚族(Ⅰ、Ⅱ、Ⅲ、ⅣA和ⅣB),Zx CNGC5属于第Ⅱ亚族;组织特异性分析显示该基因主要在根中表达;在0.01和10 mmol·L^(-1) CaCl_2处理下,ZxCNGC5在根中的表达量短期内均显著升高,并分别在3和12 h达到最高,随后逐渐降低,表明ZxCNGC5在根中的表达受外界CaCl_2的短期诱导。 | 刘亚琪 王文颖 崔彦农 郭欢 李孟湛 王锁民 | 2018 | 植物生理学报2018，54，1： | 3 |
| 4 | 植物内环核苷酸门控离子通道的研究进展显示文摘环核苷酸门控离子通道(CNGCs)是植物体内重要的非选择性的四聚体阳离子通道,参与植物细胞离子选择通透性、生长发育、生物和非生物胁迫响应等多种生物学功能的调控。本文简要介绍了植物中CNGCs的分类和定位,并阐述了CNGCs的结构和其异四聚体组装的最新进展,总结了近年来CNGCs在植物生长发育、对盐、干旱、冷、热和重金属等非生物胁迫的耐性及抗病反应等生物学功能方面的研究,讨论了CNGCs关于被cNMP激活调控的争议以及磷酸化和CaM调控模式的最新研究进展,为深度发掘其功能奠定基础。 | 李可新 赵芊 丁一 宋水山 | 2021 | 植物生理学报2021，57，12： | 2 |
| 5 | Three CNGC Family Members, CNGC5, CNGC6, and CNGC9, Are Required for Constitutive Growth of Arabidopsis Root Hairs as Ca2+-Permeable Channels显示文摘The genetic identities of Ca2+ channels in root hair (RH) tips essential for constitutive RH growth have remained elusive for decades. Here, we report the identification and characterization of three cyclicnucleotide-gated channel (CNGC) family members, CNGC5, CNGC6, and CNGC9, as Ca2+ channels essential for constitutive RH growth in Arabidopsis. We found that the cngc5-1cngc6-2cngc9-1 triple mutant(designated shrh1) showed significantly shorter and branching RH phenotypes as compared with thewild type. The defective RH growth phenotype of shrh1 could be rescued by either the expression ofCNGC5, CNGC6, or CNGC9 single gene or by the supply of high external Ca2+, but could not be rescuedby external K+ supply. Cytosolic Ca2+ imaging and patch-clamp data in HEK293T cells showed that thesethree CNGCs all function as Ca2+-permeable channels. Cytosolic Ca2+ imaging in growing RHs furthershowed that the Ca2+ gradients and their oscillation in RH tips were dramatically attenuated in shrh1compared with those in the wild type. Phenotypic analysis revealed that these three CNGCs are Ca2+ channels essential for constitutive RH growth, with different roles in RHs from the conditional player CNGC14.Moreover, we found that these three CNGCs are involved in auxin signaling in RHs. Taken together, ourstudy identified CNGC5, CNGC6, and CNGC9 as three key Ca2+ channels essential for constitutive RHgrowth and auxin signaling in Arabidopsis. | Yan-Qiu Tan Yang Yang An Zhang Cui-Fang Fei Li-Li Gu Shu-Jing Sun Wei Xu Lingling Wang Hongtao Liu Yong-Fei Wang | 2020 | Plant Communications2020，1，1： | 1 |
| 6 | 水稻CSC11介导干热风/干旱诱导的钙信号调控雄蕊发育显示文摘【目的】水稻花期偶遇干热风/干旱,导致脆弱的生殖细胞快速失水,极大地降低产量,这一过程中钙离子作为通用的第二信使传导了干旱或其他逆境信号,但背后的分子机制尚不清楚。分析钙离子透过性胁迫反应阳离子通道家族(calcium-permeable stress-responsive cation channels,CSCs)基因的生理和分子功能,为研究作物干热风的感应机制提供新的理论基础和思路。【方法】采用电生理学和遗传学方法,利用双电极电压钳技术在水稻中鉴定得到一个具有典型特征的受体类-钙通道蛋白,名为OsCSC11,对其蛋白序列进行生物信息学和进化关系分析。运用qRT-PCR和GUS报告基因活性分析确认OsCSC11的表达模式,在拟南芥原生质体细胞和洋葱表皮细胞中瞬时表达OsCSC11-GFP融合蛋白,验证OsCSC11的亚细胞定位;同时利用CRISPR/Cas9基因编辑技术获得OsCSC11的突变体,并通过细胞学等手段分析突变体表型和相关生理功能。【结果】蛋白序列比对发现,OsCSC11具有CSCs家族成员典型的保守结构域DUF221,但与其他成员序列差异大,存在不同于其他成员的特异结构域(motif)属于独立的亚家族。OsCSC11主要在水稻的花药和叶片中表达,进一步分析发现全长OsCSC11处于静息状态,可被高渗透溶液激活;但是删除N端156氨基酸(TM1-3)之后的OsCSC11^(ΔTM1-3)具有组成型的通道活性,特异选择钙、镁二价阳离子;推测TM1-3是这类通道的受体结构域,感应干热风胁迫,而OsCSC11^(ΔTM1-3)区域负责钙信号产生。OsCSC11和OsCSC11^(ΔTM1-3)均定位在细胞质膜上,与其干热风的受体功能相适应。与野生型相比,功能缺失突变体oscsc11-1和oscsc11-2的雄蕊较小、花药表面蹙皱,整体多呈弯曲状态,花粉含水量较低,败育率高达60%—70%。【结论】OsCSC11是水稻感应短期干热风/干旱刺激、介导钙离子内流,调控花药水分状态和花粉发育的受体类钙通道,可能参与了水稻雄蕊应对干热风的原初感应过程。 | 任志杰 李倩 孙钰佳 孔冬冬 刘良玉 侯聪聪 李乐攻 | 2021 | 中国农业科学2021，54，10： | 0 |
| 7 | 植物环核苷酸门控离子通道的研究进展显示文摘环核苷酸是植物生命活动过程中重要信号通路的信号分子,植物环核苷酸门控离子通道(Cyclic nucleotide-gated channels,CNGCs)的活性可由环核苷酸调控,参与植物的生长发育以及响应各种胁迫等重要生理过程。从植物环核苷酸门控离子通道的分子结构特性、调节和离子选择性以及功能进行了详细综述,总结植物环核苷酸门控离子通道在植物生长发育以及胁迫反应的新进展,对今后植物环核苷酸门控离子通道的研究方向进行展望,以期为植物环核苷酸门控离子通道研究提供参考。 | 王欣悦 刘培源 余冰清 张艳峰 丁百香 严汉池 | 2021 | 湖北农业科学2021，60，8： | 0 |
| 8 | 植物CNGC功能研究进展显示文摘从结构上看,环状核苷酸门控离子通道(CNGC)是孔-环阳离子通道的超家族,广泛存在于动植物中。根据系统亲缘关系,拟南芥CNGC家族可分为5个亚家族群,它们在抗病、花粉管生长、抗冷热胁迫、抵抗重金属离子毒害和抗盐抗旱等多种信号途径中发挥重要作用,协助植物细胞应对各种生物与非生物胁迫。主要对CNGC基因家族的结构和生物学功能进行综述性介绍,并对近年来关于该基因家族的最新研究进展进行系统阐述,为深入理解CNGC基因家族在植物生长和发育过程中的重要作用提供帮助。 | 任俊达 王靖萱 崔宇星 王春 李玮瑜 杜亮 | 2021 | 安徽农业大学学报2021，48，6： | 0 |
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