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您的检索式：作者名="Aaron R.Wheeler"
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| 1 | A microfluidic platform for continuous monitoring of dopamine homeostasis in dopaminergic cells显示文摘Homeostasis of dopamine,a classical neurotransmitter,is a key indicator of neuronal health.Dysfunction in the regulation of dopamine is implicated in a long list of neurological disorders,including addiction,depression,and neurodegeneration.The existing methods used to evaluate dopamine homeostasis in vitro are inconvenient and do not allow for continuous non-destructive measurement.In response to this challenge,we introduce an integrated microfluidic system that combines dopaminergic cell culture and differentiation with electroanalytical measurements of extracellular dopamine in real-time at any point during an assay.We used the system to examine the behavior of differentiated SH-SY5Y cells upon exposure to four dopamine transporter ant/agonists(cocaine,ketamine,epigallocatechin gallate,and amphetamine)and study their pharmacokinetics.The IC_(50)values of cocaine,ketamine,and epigallocatechin gallate were determined to be(average±standard deviation)3.7±1.1μM,51.4±17.9μM,and 2.6±0.8μM,respectively.Furthermore,we used the new system to study amphetamine-mediated dopamine release to probe the related phenomena of dopamine transporter-mediated reverse-transport and dopamine release from vesicles.We propose that this platform,which is the first platform to simultaneously evaluate uptake and release,could be useful to screen for drugs and other agents that target dopaminergic neurons and the function of the dopamine transporter.More broadly,this platform should be adaptable for any application that could benefit from high-temporal resolution electroanalysis combined with multi-day cell culture using small numbers of cells. | Yue Yu Richard P.Sde Campos Seolim Hong Dimitar L.Krastev Siddharth Sadanand Yen Leung Aaron R.Wheeler | 2019 | Microsystems & Nanoengineering2019，5，1： | 1 |
| 2 | Influence of light pattern thickness on the manipulation of dielectric microparticles by optoelectronic tweezers显示文摘Optoelectronic tweezer(OET) is a useful optical micromanipulation technology that has been demonstrated for various applications in electrical engineering and most notably cell selection for biomedical engineering. In this work, we studied the use of light patterns with different shapes and thicknesses to manipulate dielectric microparticles with OET. It was demonstrated that the maximum velocities of the microparticles increase to a peak and then gradually decrease as the light pattern’s thickness increases. Numerical simulations were run to clarify the underlying physical mechanisms, and it was found that the observed phenomenon is due to the co-influence of horizontal and vertical dielectrophoresis forces related to the light pattern’s thickness. Further experiments were run on light patterns with different shapes and objects with different sizes and structures. The experimental results indicate that the physical mechanism elucidated in this research is an important one that applies to different light pattern shapes and different objects, which is useful for enabling users to optimize OET settings for future micromanipulation applications. | Shuailong Zhang Mohamed Elsayed Ran Peng Yujie Chen Yanfeng Zhang Steven L.Neale Aaron R.Wheeler | 2022 | Photonics Research2022，10，2： | 0 |
| 3 | Correction to:A microfluidic platform for continuous monitoring of dopamine homeostasis in dopaminergic cells显示文摘Correction to:Microsystems&Nanoengineering(2019)5:10 http://gffzzd3cc09b8251d45dfs9v9wb5qn5nwv6cv9.ffgz.tsg.suse.edu.cn/10.1038/s41378-019-0049-2 published online:11 March 2019 The legend in Fig.4a in the previously published version of this article contained erroneous units.The correct units are given in the caption–that is,1μM(black),500 nM(red),100 nM(blue),50 nM(cyan),10 nM(pink),and 0 nM(1×PBS solution,brown). | Yue Yu Richard P.Sde Campos Seolim Hong Dimitar L.Krastev Siddharth Sadanand Yen Leung Aaron R.Wheeler | 2019 | Microsystems & Nanoengineering2019，5，1： | 0 |
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