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| 1 | Bcl-x_L and Myeloid cell leukaemia-1 contribute to apoptosis resistance of colorectal cancer cells显示文摘AIM: To explore the role of Bcl-xL and Myeloid cell leukaemia (Mcl)-1 for the apoptosis resistance of colorectal carcinoma (CRC) cells towards current treat-ment modalities. METHODS: Bcl-xL and Mcl-1 mRNA and protein ex-pression were analyzed in CRC cell lines as well as human CRC tissue by Western blot,quantitative PCRand immunohistochemistry. Bcl-xL and Mcl-1 protein expression was knocked down or increased in CRC cell lines by applying specific siRNAs or expression plas-mids,respectively. After modulation of protein expres-sion,CRC cells were treated with chemotherapeutic agents,an antagonistic epidermal growth factor recep-tor (EGFR1) antibody,an EGFR1 tyrosine kinase inhibi-tor,or with the death receptor ligand TRAIL. Apoptosis induction and cell viability were analyzed. RESULTS: Here we show that in human CRC tis-sue and various CRC cell lines both Bcl-xL and Mcl-1 are expressed. Bcl-xL expression was higher in CRC tissue than in surrounding non-malignant tissue,both on protein and mRNA level. Mcl-1 mRNA expression was significantly lower in ma-lignant tissues. However,protein expression was slightly higher. Viability rates of CRC cells were significantly decreased after knock down of Bcl-xL expression,and,to a lower extent,after knock down of Mcl-1 expression. Furthermore,cells with reduced Bcl-xL or Mcl-1 expression was more sensitive towards oxaliplatin-and irinotecan-induced apoptosis,and in the case of Bcl-xL also towards 5-FU-induced apoptosis. On the other hand,upregulation of Bcl-xL by transfec-tion of an expression plasmid decreased chemothera-peutic drug-induced apoptosis. EGF treatment clearly induced Bcl-xL and Mcl-1 expression in CRC cells. Apop-tosis induction upon EGFR1 blockage by cetuximab or PD168393 was increased by inhibiting Mcl-1 and Bcl-xL expression. More strikingly,CD95-and TRAIL-induced apoptosis was increased by Bcl-xL knock down. CONCLUSION: Our data suggest that Bcl-xL and,to a lower extent,Mcl-1,are important anti-apoptotic factors in CRC. Specific downregulation of Bcl-xL is a promising approach to sensitize CRC cells towards chemotherapy and targeted therapy. | Henning Schulze-Bergkamen Roland Ehrenberg Lothar Hickmann Binje Vick Toni Urbanik Christoph C Schimanski Martin R Berger Arno Schad Achim Weber Steffen Heeger Peter R Galle Markus Moehler | 2008 | World Journal of Gastroenterology2008，14，24： | 4 |
| 2 | Isolated subcutaneous implantation of a borderline ovarian tumor:A case report and review of the literature显示文摘Laparoscopy-related tumor implantations of gynecological malignancies into the subcutaneous tissue are rarely diagnosed.We report an interesting case of a 46-year-old female who presented with an abdominal subcutaneous metastasis of a borderline ovarian tumor.The patient received a laparoscopic unilateral adnexectomy for a solid-cystic tumor of the right ovary.Histopathological workup showed a papillary borderline tumor of mucinous type.Nine days later she underwent a hysterectomy,left adnexectomy,appendectomy and omentectomy.Exploration of the peritoneum revealed no intraperitoneal implants.Further exploration showed a non-invasive implant of a borderline tumor in the subcutaneous tissue above the fascia that had no contact to the peritoneum.It is hypothesized that tumor cells may have been implanted during a previous laparoscopy,the most recent of which had been fourteen years prior to her current presentation.Various risk factors for port-site malignancies have been identified.Tumor manipulation and extraction of tumor tissue without a protective bag may contribute to development of trocarsite metastasis. | Malgorzata Banys-Paluchowski Borsu Yeganeh Jutta Luettges Achim Maibach Ruediger Langenberg Natalia Krawczyk Peter Paluchowski Holger Maul Gerhard Gebauer | 2016 | World Journal of Clinical Oncology2016，7，2： | 4 |
| 3 | Stability analysis of a thin-walled cylinder in turning operation using the semi-discretization method显示文摘In this work, the stability of a flexible thin cylindrical workpiece in turning is analyzed. A process model is derived based on a finite element representation of the workpiece flexibility and a nonlinear cutting force law. Repeated cutting of the same surface due to overlapping cuts is modeled with the help of a time delay. The stability of the so obtained system of periodic delay differential equations is then determined using an approximation as a time-discrete system and Floquet theory. The time-discrete system is obtained using the semi-discretization method. The method is implemented to analyze the stability of two different workpiece models of different thicknesses for different tool positions with respect to the jaw end. It is shown that the stability chart depends on the tool position as well as on the thickness. | Arnab Chanda Achim Fischer Peter Eberhard Santosha Kumar Dwivedy | 2014 | Acta Mechanica Sinica2014，30，2： | 3 |
| 4 | 小型激光天文动力学空间计划概念显示文摘小型激光天文动力学空间计划是 :使用在太阳轨道上无拖曳航天器和地面站以激光干涉和脉冲测距的方法 ,精确地探讨天文动力学 ,检测相对论与时空基本定律 ,改进探测引力波的灵敏度以及更准确地测定太阳、行星和小行星的参数。 1 969年开始的月球激光 (反射 )测距 ,对地球物理、参考坐标的选定、相对论的检验均有重要的贡献。 3 0年来 ,激光技术的长足进步 ,使现在正是适合于开始进行研究空间有源 (主动 )测距和光波空间通讯的时候。激光天文动力学的兴起是必然的趋势 ,其精确度将比现在提高 3到 6个数量级 ,将是天文动力学革命性的发展。小型激光天文动力学空间计划可以起到带头作用。它的关键技术有三 ,即 :弱光锁相、极精确无拖曳航天和高衰减日冕仪。弱光锁相已有长足的进步。对高衰减日冕仪的研究 ,也有了初步的方案。LISA空间计划将于 2 0 0 6年 8月发射SMART -2 ,研究测试极精确无拖曳航天。小型激光天文动力学空间计划的关键技术已日趋成熟。在第一届国际激光天文动力学研讨会 ( 2 0 0 1 ,9.1 3 -2 3 )中介绍了各相关学科背景及前沿研究 ,讨论了激光天文动力学空间计划科学目标及相关技术 ,并召开了两次小型激光天文动力学空间计划预研究筹备会 ,建立了和欧洲的合作关系。会后着手进行此项对基础? | 倪维斗 朱进 武向平 褚桂柏 杨彬 高健 关敏 汤健仁 周翊 张中豪 黄天衣 曲钦岳 易照华 李广宇 陶金河 吴岸明 罗俊 叶贤基 周泽兵 熊耀恒 毕少兰 须重明 吴雪君 唐孟希 包芸 李芳昱 黄珹 杨福民 叶叔华 张书练 张元仲 聂玉昕 陈光 Joergen Christensen-Dalsgaard Hansjoerg Dittus Yasunori Fujii Claus Laemmerzahl Jean Francois Mangin Achim Peters Albrecht Ruediger Etienne Samain Stephan Schiller | 2002 | 云南天文台台刊2002，，3： | 3 |
| 5 | Efficacy of SpyGlass^(TM)-directed biopsy compared to brush cytology in obtaining adequate tissue for diagnosis in patients with biliary strictures显示文摘AIM: To evaluate the diagnostic yield(inflammatory activity) and efficiency(size of the biopsy specimen) of SpyGlassTM-guided biopsy vs standard brush cytology in patients with and without primary sclerosing cholangitis(PSC).METHODS: At the University Medical Center Mainz, Germany, 35 consecutive patients with unclear biliarylesions(16 patients) or long-standing PSC(19 patients) were screened for the study. All patients underwent a physical examination, lab analyses, and abdominal ultrasound. Thirty-one patients with non-PSC strictures or with PSC were scheduled to undergo endoscopic retrograde cholangiography(ERC) and subsequent per-oral cholangioscopy(POC). Standard ERC was initially performed, and any lesions or strictures were localized. POC was performed later during the same session. The Boston Scientific SpyGlass SystemTM(Natick, MA, United States) was used for choledochoscopy. The biliary tree was visualized, and suspected lesions or strictures were biopsied, followed by brush cytology of the same area. The study endpoints(for both techniques) were the degree of inflammation, tissue specimen size, and the patient populations(PSC vs non-PSC). Inflammatory changes were divided into three categories: none, low activity, and high activity. The specimen quantity was rated as low, moderate, or sufficient.RESULTS: SpyGlassTM imaging and brush cytology with material retrieval were performed in 29 of 31(93.5%) patients(23 of the 29 patients were male). The median patient age was 45 years(min, 20 years; max, 76 years). Nineteen patients had known PSC, and 10 showed non-PSC strictures. No procedure-related complications were encountered. However, for both methods, tissues could only be retrieved from 29 pa-tients. In cases of inflammation of the biliary tract, the diagnostic yield of the SpyGlassTM-directed biopsies was greater than that using brush cytology. More tissue material was obtained for the biopsy method than for the brush cytology method(P = 0.021). The biopsies showed significantly more inflammatory characteristics and greater inflammatory activity compared to the cy-tological investigation(P = 0.014). The greater quantity of tissue samples proved useful for both PSC and non-PSC patients.CONCLUSION: SpyGlassTM imaging can be recom-mended for proper inflammatory diagnosis in PSC pa-tients. However, its value in diagnosing dysplasia wasnot addressed in this study and requires further investi-gation. | Johannes Wilhelm Rey Torsten Hansen Sebastian Dümcke Achim Tresch Katja Kramer Peter Robert Galle Martin Goetz Marcus Schuchmann Ralf Kiesslich Arthur Hoffman | 2014 | World Journal of Gastrointestinal Endoscopy2014，6，4： | 2 |
| 6 | Addressing concerns about cisplatin application during pregnancy显示文摘 | Malgorzata Lanowska Christhardt K?hler Peter Oppelt Alexander Schmittel Elisabeth Gottschalk Kati Hasenbein Achim Schneider Simone Marnitz | 2011 | Journal of Perinatal Medicine2011，，3： | 2 |
| 7 | Accuracy of frozen section exami- nation of testicular tumors of uncertain origin显示文摘 | Achim E Peter O Axel H | 2002 | Euro Urol2002，41，3： | 1 |
| 8 | Orbital and solar forcing of shifts in Mid- to Late Holocene flood intensity from varved sediments of pre-alpine Lake Ammersee (southern Germany)显示文摘 | Markus Czymzik Achim Brauer Peter Dulski Birgit Plessen Rudolf Naumann Ulrich von Grafenstein Raphael Scheffler | 2013 | Quaternary Science Reviews2013，，： | 1 |
| 9 | Specific and sensitive quantification of folate vitamers in foods by stable isotope dilution assays using high performance liquid chromatography-tandem mas spectrometry 显示文摘 | Achim F Peter S Michael R | 2003 | Anal Bioanal Chem2003，，376： | 1 |
| 10 | The Influence of DISI Engine Operating Parameters on Particle Number Emissions显示文摘 | Daniel Sabathil Achim Koenigstein Peter Schaffl~er Jan Fritzsche and Arndt Doehler | 2011 | SAE Paper2011，，1： | 1 |
| 11 | Structural Steel and Plane Frame Assemblies under Fire Action 显示文摘 | Achim Rubert and Peter Schanmann | 1986 | Fire Safety Journal1986，，10： | 1 |
| 12 | Measurement of gravitational acceleration by dropping atoms 显示文摘 | Achim Peters Keng Yeow Chung Steven Chu | 1999 | Nature1999，400，： | 1 |
| 13 | Ki-67 as a prognostic molecular marker in routine clinical use in breast cancer patients显示文摘 | Folkward G. Wiesner Achim Magener Peter A. Fasching Julia Wesse Mayada R. Bani Claudia Rauh Sebastian Jud Michael Schrauder Christian R. Loehberg Matthias W. Beckmann Arndt Hartmann Michael P. Lux | 2009 | The Breast2009，，2： | 1 |
| 14 | Critical review: vegetables and fruit in the prevention of chronic diseases显示文摘 | Heiner Boeing Angela Bechthold Achim Bub Sabine Ellinger Dirk Haller Anja Kroke Eva Leschik-Bonnet Manfred Müller Helmut Oberritter Matthias Schulze Peter Stehle Bernhard Watzl | 2012 | European Journal of Nutrition2012，，6： | 1 |
| 15 | Impact of short-term versus long-term topical steroids on corneal neovascularization after non-high-risk keratoplasty显示文摘 | Claus Cursiefen Hartmut Wenkel Peter Martus Achim Langenbucher Nhung X. Nguyen Berthold Seitz Michael Küchle Gottfried O. Naumann | 2001 | Graefe’s Archive for Clinical and Experimental Ophthalmology2001，，7： | 1 |
| 16 | Neuromuscuar Evaluation of Tnmk- Training Exercises 显示文摘 | Peter Konrad Klaus Schmitz Achim Denner | | Journal of Athletic Trainning0，，： | 1 |
| 17 | Improving the dynamic stability of a workpiece dominated turning process using an adaptronic tool holder显示文摘In this contribution, inside turning of a thin-walled cylinder is investigated in simulation. Self-excited vibrations can arise due to repeated cutting of the same surface, that lead to instability. A flexible multibody system model of the system is the basis for a subsequent analysis of the stability of the process. Stability analysis is done using an approximation as a time-discrete system via the semi-discretization method. An adaptronic turning chisel comprising a piezo actuator and sensors is then used in combination with different control concepts to improve the stability of the process. The effectiveness of the different strategies is compared based on the influence on the stability charts. A classic H ∞ controller based on a model of the coupled system of workpiece and tool can only yield some improvements, when an additional measurement of the workpiece displacement is added. Incorporating knowledge on the cutting process coupling workpiece and tool using a gain scheduled H ∞ controller allows further improvements. However, robustness with respect to model uncertainties,notably concerning the force law, remains an issue. | Achim Fischer Peter Eberhard | 2013 | Theoretical & Applied Mechanics Letters2013，3，1： | 1 |
| 18 | Dendritic cell-based tumor vaccine for cervical cancer II: results of a clinical pilot study in 15 individual patients显示文摘 | Alfonso Ferrara Marion Nonn Peter Sehr Carola Schreckenberger Michael Pawlita Matthias Dürst Achim Schneider Andreas M. Kaufmann | 2003 | Journal of Cancer Research and Clinical Oncology2003，，9： | 1 |
| 19 | Diabetes and Peripheral Arterial Occlusive Disease:Prospective Comparison of Contrast-Enhanced Three-Dimensional MR Angiography with Conventional Digital Subtraction Angiography显示文摘 | Karl-Friedrich Kreitner Peter Kalden Achim Neufang | 2000 | AJR Am J Roentgenol2000，174，1： | 1 |
| 20 | Effect of TNF gene depletion on liver regeneration after partial hepatectomy in mice显示文摘 | Junya Fujita Michael W. Marino Hisashi Wada Achim A. Jungbluth Peter J. Mackrell David E. Rivadeneira Philip P. Stapleton John M. Daly | 2001 | Surgery2001，，1： | 1 |
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