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| 1 | Low temperature induced changes in activity and protein levels of the enzymes associated to conversion of starch to sucrose in banana fruit显示文摘 | Roberta Ghedini Der Agopian Fernanda Helena Gon?alves Peroni-Okita Claudinéia Aparecida Soares Janaína Aparecida Mainardi Jo?o Roberto Oliveira do Nascimento Beatriz Rosana Cordenunsi Franco Maria Lajolo Eduardo Purgatto | 2011 | Postharvest Biology and Technology2011，，2： | 2 |
| 2 | Evidence Supporting a Key Role of Lp-PLA2-Generated Lysophosphatidylcholine in Human Atherosclerotic Plaque Inflammation显示文摘 | Isabel Gon?alves Andreas Edsfeldt Na Young Ko Helena Grufman Katarina Berg Harry Bj?rkbacka Mihaela Nitulescu Ana Persson Marie Nilsson Cornelia Prehn Jerzy Adamski Jan Nilsson | 2012 | Arteriosclerosis, Thrombosis, and Vascular Biology2012，，6： | 1 |
| 3 | Analysis of a hydrometallurgical route to recover base metals from spent rechargeable batteries by liquidliquid extraction with Cyanex 272显示文摘 | Germano Dorella Renata Cristi na Alves Elias | 2006 | Journal of Power Sources2006，159，2： | 1 |
| 4 | Determining soil salinity from soil electrical conductivity using different models and estimates显示文摘 | Rhoades JD Shouse PJ Alves NA | 1990 | Soil Science1990，54，： | 1 |
| 5 | Antibacterial activity and inhibition against Staphylococcus aureus NorA efflux pump by ferulic acid and its esterified derivatives显示文摘Objective:To evaluate the inhibitory activity of ferulic acid and four of its esterified derivatives(methyl,ethyl,propyl,and butyl)against resistance mechanisms in Staphylococcus aureus strains.Methods:Ferulic acid derivatives were obtained by esterification with methanol,ethanol,propanol,and butanol,and then characterized by hydrogen and carbon-13 nuclear magnetic resonance analysis.The minimum inhibitory concentrations(MIC)of ferulic acid and its esterified derivatives,ethidium bromide,and norfloxacin were obtained using the microdilution test,while the efflux pump inhibition test was conducted by examining reduction in the MICs of norfloxacin and ethidium bromide.Molecular docking was also carried out using the Schrodinger Suite 2015 molecular modeling software.A three-dimensional model of NorA efflux pump was generated using I-TASSER.The best scoring model was used as a receptor for ligand-receptor docking.Results:The methyl and butyl ester derivatives did not demonstrate significant antimicrobial activity.However,a significant synergic effect was evidenced when norfloxacin was combined with the ethyl and propyl esterified derivatives.The docking study demonstrated favorable energy of interaction between ferulate derivatives and NorA,and amino acid residues TYR57,TYR58,and LEU255 were present commonly in stabilizing all complexes.The PCA analysis corroborated the docking hypothesis that the lipophilic character and hydrogen bond interactions were the most relevant characteristics involved with NorA inhibitors.The pharmacokinetic parameters of ferulic acid derivatives showed good ADMET properties,demonstrating that they can be easily absorbed and have no effect or inhibit the cytochrome P450 enzyme complex,revealing their potential as drug candidates.Conclusions:This study provides strong evidence that the molecular basis for this activity is potentially due to the NorA efflux pump. | Patrícia Gonçalves Pinheiro Gilvandete Maria Pinheiro Santiago Francisco Erivaldo Freitas da Silva Ana Carolina Justino de Araújo Cícera Rejane Tavares de Oliveira Priscilla Ramos Freitas Janaína Esmeraldo Rocha JoséBezerra de Araújo Neto Maria Milene Costa da Silva Saulo Relison Tintino Irwin Rose Alencar de Menezes Henrique Douglas Melo Coutinho JoséGalberto Martins da Costa | 2021 | Asian Pacific Journal of Tropical Biomedicine2021，11，9： | 0 |
| 6 | Cloning and characterization of gene-resistant analogs(RGAs)involved in rust(Puccinia psidii) resistance in Eucalyptus grandis显示文摘Disease-resistant genes play an important role in defending against a variety of pathogens and insect pests in plants. Most of the disease-resistant genes encode proteins with conserved leucine rich repeat and nucleotide binding site domains. In this study, we cloned and characterized gene-resistant analogs(RGAs) from Eucalyptus grandis using degenerate PCR, with primers specifically targeting these two domains. The amplified fragments were cloned into the p GEM-T vector and transformed into Escherichia coli. Among the 90 clones obtained, 13 were sequenced and compared with each other and with previously identified gene-resistant diseases. A BLASTX search in Gen Bank revealed high similarities among the conserved domains of these cloned genes with RGA genes.Some clones, however, showed no significant similarity with DNA sequences in Gen Bank. Southern blotting analysis identified several polymorphic RFLP loci between distinct genotypes. However, none of them co-segregated with the Puccinia psidii Winter resistance gene 1(Ppr1) in a population study. | Marcelo Luiz Laia Acelino Couto Alfenas Sergio Hermínio Brommonschenkel Shinitiro Oda Eduardo José de Melo Inaê Mariêde Araú jo Silva Janaína Fernandes Gonalves Ariadne Marques | 2015 | Journal of Forestry Research2015，26，3： | 0 |
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