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| 1 | Developmental dysplasia of the hip in the newborn: A systematic review显示文摘Developmental dysplasia of the hip(DDH) denotes a wide spectrum of conditions ranging from subtle acetabular dysplasia to irreducible hip dislocations. Clinical diagnostic tests complement ultrasound imaging in allowing diagnosis, classification and monitoring of this condition. Classification systems relate to the alpha and beta angles in addition to the dynamic coverage index(DCI). Screening programmes for DDH show considerable geographic variation; certain risk factors have been identified which necessitate ultrasound assessment of the newborn. The treatment of DDH has undergone significant evolution, but the current gold standard is still the Pavlik harness. Duration of Pavlik harness treatment has been reported to range from 3 to 9.3 mo. The beta angle, DCI and the superior/lateral femoral head displacement can be assessed via ultrasound to estimate the likelihood of success. Success rates of between 7% and 99% have been reported when using the harness to treat DDH. Avascular necrosis remains the most devastating complication of harness usage with a reported rate of between 0% and 28%. Alternative non-surgical treatment methods used for DDH include devices proposed by LeD amany, Frejka, Lorenz and Ortolani. The Rosen splint and Wagner stocking have also been used for DDH treatment. Surgical treatment for DDH comprises open reduction alongside a combination of femoral or pelvic osteotomies. Femoral osteotomies are carried out in cases of excessive anteversion or valgus deformity of the femoral neck. The two principal pelvic osteotomies most commonly performed are the Salter osteotomy and Pemberton acetabuloplasty. Serious surgical complications include epiphyseal damage, sciatic nerve damage and femoral neck fracture. | Vivek Gulati Kelechi Eseonu Junaid Sayani Nizar Ismail Chika Uzoigwe Muhammed Zaki Choudhury Pooja Gulati Adeel Aqil Saket Tibrewal | 2013 | World Journal of Orthopedics2013，4，2： | 29 |
| 2 | Determination of blast-induced ground vibration equations for rocks using mechanical and geological properties显示文摘In the recent decades,effects of blast loads on natural and man-made structures have gained considerable attention due to increase in threat from various man-made activities.Site-specific empirical relationships for calculation of blast-induced vibration parameters like peak particle velocity(PPV) and peak particle displacement(PPD) are commonly used for estimation of blast loads in design.However,these relationships are not able to consider the variation in rock parameters and uncertainty of in situ conditions.In this paper,a total of 1089 published blast data of various researchers in different rock sites have been collected and used to propose generalized empirical model for PPV by considering the effects of rock parameters like unit weight,rock quality designation(RQD),geological strength index(GSI),and uniaxial compressive strength(UCS).The proposed PPV model has a good correlation coefficient and hence it can be directly used in prediction of blast-induced vibrations in rocks.Standard errors and coefficient of correlations of the predicted blast-induced vibration parameters are obtained with respect to the observed field data.The proposed empirical model for PPV has also been compared with the empirical models available for blast vibrations predictions given by other researchers and found to be in good agreement with specific cases. | Ranjan Kumar Deepankar Choudhury Kapilesh Bhargava | 2016 | Journal of Rock Mechanics and Geotechnical Engineering2016，8，3： | 11 |
| 3 | 小胶质细胞可能通过选择性吞噬丘脑底核谷氨酸能突触来代偿多巴胺能神经元丢失造成的帕金森模型鼠的功能缺失显示文摘当帕金森患者出现临床症状时,其黑质致密部的绝大部分多巴胺神经元已发生退行性改变。这提示可能存在某些代偿机制,延缓了临床症状的出现。本研究制作6-羟多巴胺诱导的偏侧帕金森大鼠模型,观察位于黑质下网状部和苍白球的激活的小胶质细胞在代偿机制中的作用。研究发现,在偏侧帕金森大鼠中,黑质下网状部聚集的激活的小胶质细胞远多于苍白球中。激活的小胶质细胞胞体增大并表达吞噬物标志物CD68和NG2蛋白多糖。激活的小胶质细胞聚集在黑质下网状部的特定部位,该部位的突触蛋白I和突触后密集蛋白95的表达降低。活化的小胶质细胞吞噬突触前、后膜上的各种蛋白,包括NMDA受体。将红色荧光标记物Di I作为顺行示踪剂注入丘脑底核,可见黑质下网状部和苍白球内的细胞吞噬了Di I。在谷氨酸刺激下,原代培养的小胶质细胞吞噬活动增强,编码吞噬相关因子的m RNA转录水平也增加。人工合成的糖皮质激素地塞米松可以减弱这种改变。给PD大鼠皮下注射地塞米松,可以抑制黑质下网状部小胶质细胞的活化,运动功能障碍进一步加重,谷氨酸能突触转录m RNA增加,GABA能突触转录m RNA水平未增加。这些发现提示,当谷氨酸能神经元活动增强时,黑质下网状部和苍白球的小胶质细胞活化,选择性吞噬丘脑底核的谷氨酸能突触。因此,在基底核区可能存在一个负反馈环路,间接地代偿了由于多巴胺能神经元丢失而导致的功能障碍。小胶质细胞在这个负反馈环路中扮演了重要的角色。 | Aono H Choudhury ME Higaki H Miyanishi K Kigami Y Fujita K Akiyama JI Takahashi H Yano H Kubo M Nishikawa N Nomoto M Tanaka J 唐颖馨 | 2017 | 神经损伤与功能重建2017，12，5： | 7 |
| 4 | Life style modification improves insulin resistance and liver histology in patients with non-alcoholic fatty liver disease显示文摘AIM: To study the effect of regular aerobic exercise on insulin resistance, serum aminotransferase and liver histology in nonalcoholic fatty liver disease (NAFLD) patients. METHODS: Sixty (mean age 40.0 ± 8.5 years, 75% male) NAFLD patients were included in the study. After baseline anthropometric measurement i.e., body mass index (BMI), waist circumference (WC); all patients were advised regular aerobic exercise for 30 min/d, for at least 5 d/wk and trained to achieve around 70% of his maximal heart rate. In addition, moderately energy restricted diet was advised to patients with high BMI (> 25 kg/m 2 ). Monthly follow up was done by measuring BMI, WC, aspartate aminotransferase, and alanine aminotransferase (ALT). Insulin resistance was calculated using homeostasis model assessment (HOMA) of insulin resistance (HOMA-IR) model, at baseline and after 6mo. Insulin resistance was arbitrarily considered altered when it was ≥ 2. Liver biopsy was done in a section of patients at baseline and after 6 mo. RESULTS: Seventy percent (42/60) patients were overweight or obese; 95% (57/60) had central obesity (WC > 90 cm in men, > 80 cm in women). In the 45 exercise compliant patients insulin resistance decreased from 6.4 ± 6.1 to 1.3 ± 1.0, BMI from 26.7 ± 3.3 kg/m 2 to 25.0 ± 3.3 kg/m 2 , WC from 95.7 ± 8.9 cm to 90.8 ± 7.3 cm and ALT from 84.8 ± 43.5 U/L to 41.3 ± 18.2 U/L (P < 0.01). In 15 exercise noncompliant patient's insulin resistance, BMI, WC and ALT did not show significant change at 6 mo follow up. Six of 8 patients in compliant group on repeat liver biopsy showed significant change in steatosis and necroinflammation. Nonalcoholic steatohepatitis scores improved form 5.3 ± 1.5 to 3.35 ± 1.5. The decline in insulin resistance correlated with decline in ALT (P = 0.01, r s = 0.90) and liver histology (P = 0.03, r s = 0.73). CONCLUSION: Life style modification improves insulin resistance resulting in improvement in ALT and liver histology in NAFLD patients. | Ganesh Bhat Chalamalasetty Sreenivasa Baba Amaresh Pandey Neeraj Kumari Gourdas Choudhuri | 2012 | World Journal of Hepatology2012，4，7： | 5 |
| 5 | Homologue of mammalian apolipoprotein A-II in non-mammalian vertebrates显示文摘尽管有分子的重量的 apolipoprotein 14 kDa (apo-14 kDa ) 与鱼血浆被联系高密度的脂蛋白(HDL ) , apo-14 kDa 是否是哺乳动物的 apoA-II 的相当或相同事物,尚待坚定。我们获得了编码日本鳗和彩虹鳟鱼 apo-14 kDa 的完整的 cDNA 序列。日本鳗 apo-14 kDa 顺序的相当或相同事物能在在 DDBJ/EMBL/GenBank 或 TGI 数据库扔的 14 鱼种类被发现。鱼 apo-14 kDa 缺乏 propeptide 并且比哺乳动物的 apoA-II 包含更内部的重复。不过,种系发生的分析允许鱼 apo-14 kDa 是哺乳动物的 apoA-II 的相当或相同事物。在 TGI 克隆的 silico,另外, Ensembl,或 NCBI 数据库表明在狗,鸡,绿 anole 蜥蜴,和非洲人的 apoA-IIs 抓了其序列到目前为止一直不可得到的青蛙,建议两 apoA -- 我和 apoA-II 脊椎动物 HDL 的同样基本的成分。 | Malay Choudhury Shoji Yamada Masaharu Komatsu Hideki Kishimura Seiichi Ando | 2009 | Acta Biochimica et Biophysica Sinica2009，41，5： | 5 |
| 6 | Developing and Validating Trust Measures for e-Commerce: An Integrative Typology显示文摘 | D. Harrison McKnight Vivek Choudhury Charles Kacmar | 2002 | Information Systems Research2002，，3： | 4 |
| 7 | In-Situ Differentiation of Acidic and Non-Acidic Tundra via Portable X-ray Fluorescence (PXRF) Spectrometry显示文摘Frozen soils or those with permafrost cover large areas of the earth's surface and support unique vegetative ecosystems. Plants growing in such harsh conditions have adapted to small niches, which allow them to survive. In northern Alaska, USA, both moist acidic and non-acidic tundra occur, yet determination of frozen soil p Hs currently requires thawing of the soil so that electrometric pH methods can be utilized. Contrariwise, a portable X-ray fluorescence(PXRF) spectrometer was used in this study to assess elemental abundances and relate those characteristics to soil pH through predictive multiple linear regressions. Two operational modes, Soil Mode and Geochem Mode, were utilized to scan frozen soils in-situ and under laboratory conditions, respectively, after soil samples were dried and ground. Results showed that lab scanning produced optimal results with adjusted coefficient of determination(R^2) of 0.88 and 0.33 and root mean squared errors(RMSEs) of 0.87 and 0.34 between elemental data and lab-determined pH for Soil Mode and Geochem Mode, respectively. Even though the presence of ice attenuated fluoresced radiation under field conditions, adjusted R^2 and RMSEs between the datasets still provided reasonable model generalization(e.g., 0.73 and 0.49 for field Geochem Mode). Principal component analysis qualitatively separated multiple sampling sites based on elemental data provided by PXRF, reflecting differences in the chemical composition of the soils studied. Summarily, PXRF can be used for in-situ determination of soil pH in arctic environments without the need for sample modification and thawing. Furthermore, use of PXRF for determination of soil pH may provide higher sample throughput than traditional eletrometric-based methods, while generating elemental data useful for the prediction of multiple soil parameters. | Somsubhra CHAKRABORTY David C. WEINDORF GARY J. MICHAELSON Chien Lu PING Ashok CHOUDHURY Tarek KANDAKJI Autumn ACREE Akriti SHARMA WANG Dandan | 2016 | Pedosphere2016，26，4： | 4 |
| 8 | Vibrational Spectroscopy of Stretching and Bending Modes of Nickel Tetraphenyl Porphyrin: an Algebraic Approach显示文摘我们由使用 U (2 ) 为 36 个震动的乐队计算镍 tetraphenyl 卟啉的震动的频率代数学的途径。在计算的代数学的参数与试验性的数据是精确的。 | Srinivasa Rao KARUMURI Joydeep CHOUDHURY Nirmal Kumar SARKAR Ramendu BHATTACHARJEE | 2009 | Chinese Physics Letters2009，26，9： | 3 |
| 9 | Randomized clinical trial: efficacy and safety of telbivudine and lamivudine in treatment‐na?ve patients with HBV‐related decompensated cirrhosis显示文摘 | H. L.Y. Chan Y. C. Chen E. J. Gane S. K. Sarin D. J. Suh T. Piratvisuth B. Prabhakar S. G. Hwang G. Choudhuri R. Safadi T. Tanwandee A. Chutaputti C. Yurdaydin W. Bao C. Avila A. Trylesinski | 2012 | Journal of Viral Hepatitis2012，，10： | 3 |
| 10 | Microbial mat-related structures shared by both siliciclastic and carbonate formations显示文摘Microbiota has always been the dominant life form,records of which are preserved in delicate forms within siliciclastic rocks.More pronounced record in the form of stromatolites possibly obscured the fact that many of the same delicate structures may be recognizable within carbonate rocks too.The Neoproterozoic Bhander Limestone in central India bears many such structures that are quintessentially similar to microbial mat-related structures reported from the Paleoproterozoic Chorhat Sandstone preserved within the same,Vindhyan Basin.Extensive microscopic,ultramicroscopic,and geochemical studies address the apprehension that such bedding plane structures in carbonate rocks could be merely weathering products.Trapping,binding and stabilitization of sediment by microbial mats are all evident.Preferred pyritization along the inferred,predefined microbial mats confirmed on the basis of EPMA(Electron Probe Microanalysis) results,and the enhanced carbon content along these mats layers and within suspected mat chips associated with them,are revealing.Raman spectroscopy,indeed,evinces enhanced kerogen content within both mats and mat chips.Interestingly,these microbial mat layers are recognized selectively within the lower of the two tiers of the Bhander Limestone.The lagoonal carbonate of the lower tier of the Bhander Limestone is muddy and contains a substantial proportion of silt-sized quartz grains that possibly impeded stromatolite growth.Stromatolites abound in the wave agitated upper tier of the Bhander Limestone which is dominated by oosparite.This paper provides evidence that the delicate microbial mat-related structures reported so far only from siliciclastic rocks can also be recognized within carbonate formations,and hopes to stimulate the search for additional such features,more preferably within carbonates originated in shallow and quiet water. | Subir Sarkar Adrita Choudhuri Sunipa Mandal Patrick G. Eriksson | 2016 | Journal of Palaeogeography2016，5，3： | 3 |
| 11 | Effect of pioglitazone on nerve conduction velocity of the median nerve in the carpal tunnel in type 2 diabetes patients显示文摘AIM To evaluate the impact of pioglitazone pharmacotherapy in median nerve electrophysiology in the carpal tunnel among type 2 diabetes patients.METHODS The study was executed in patients with type 2 diabetes, treated with oral drugs, categorized under pioglitazone or non-pioglitazone group(14 in each group), and who received electrophysiological evaluation by nerve conduction velocity at baseline and 3 mo.RESULTS At 3 mo, pioglitazone-category had inferior amplitude in sensory median nerve [8.5 interquartile range(IQR) = 6.5 to 11.5) vs non-pioglitazone 14.5(IQR 10.5 to 18.75)](P = 0.002). Non-pioglitazone category displayed amelioration in amplitude in the sensory median nerve [baseline 13(IQR = 9 to 16.25) vs 3 mo 8.5(IQR = 6.5 to 11.5)](P = 0.01) and amplitude in motor median nerve [baseline 9(IQR = 4.75 to 11) vs 3 mo 6.75(IQR = 4.75 to 10.25)](P = 0.049); and deterioration of terminal latency of in motor ulnar nerve [baseline 2.07(IQR = 1.92 to 2.25) vs 3 mo 2.16(IQR = 1.97 to 2.325)](P = 0.043). There was amelioration of terminal latency in sensory ulnar nerve [baseline 2.45(IQR = 2.315 to 2.88) vs 3 mo 2.37(IQR = 2.275 to 2.445) for pioglitazone group(P = 0.038).CONCLUSION Treatment with pioglitazone accentuates probability of compressive neuropathy. In spite of comparable glycemic control over 3 mo, patients treated with pioglitazone showed superior electrophysiological parameters for the ulnar nerve. Pioglitazone has favourable outcome in nerve electrophysiology which was repealed when the nerve was subjected to compressive neuropathy. | Sudip Chatterjee Debmalya Sanyal Sourav Das Choudhury Mili Bandyopadhyay Suraj Chakraborty Arabinda Mukherjee | 2016 | World Journal of Diabetes2016，7，19： | 3 |
| 12 | Starspots, stellar cycles and stellar flares: Lessons from solar dynamo models显示文摘In this review, we discuss whether the present solar dynamo models can be extrapolated to explain various aspects of stellar activity. We begin with a summary of the following kinds of data for solar-like stars:(i) data pertaining to stellar cycles from Ca H/K emission over many years;(ii) X-ray data indicating hot coronal activity;(iii) starspot data(especially about giant polar spots); and(iv) data pertaining to stellar superflares. Then we describe the current status of solar dynamo modelling—giving an introduction to the flux transport dynamo model, the currently favoured model for the solar cycle. While an extrapolation of this model to solar-like stars can explain some aspects of observational data, some other aspects of the data still remain to be theoretically explained. It is not clear right now whether we need a different kind of dynamo mechanism for stars having giant starspots or producing very strong superflares. | Amab Rai Choudhuri | 2017 | Science China(Physics,Mechanics & Astronomy)2017，60，1： | 3 |
| 13 | Muskingum equation based downstream sediment flow simulation models for a river system显示文摘Applications of sediment transport and water flow characteristics based sediment transport simulation models for a river system are presented in this study.An existing water-sediment model and a new sediment-water model are used to formulate the simulation models representing water and sediment movement in a river system.The sediment-water model parameters account for water flow characteristics embodying sediment transport properties of a section.The models are revised formulations of the multiple water inflows model describing water movement through a river system as given by the Muskingum principle.The models are applied to a river system in Mississippi River basin to estimate downstream sediment concentration,sediment discharge,and water discharge.River system and the river section parameters are estimated using a revised and the original multiple water inflows models by applying the genetic algorithm.The models estimate downstream sediment transport rates on the basis of upstream sediment/water flow rates to a system.Model performance is evaluated by using standard statistical criteria;downstream water discharge resulting from the original multiple water inflows model using the estimated river system parameters indicate that the revised models satisfactorily describe water movement through a river system.Results obtained in the study demonstrate the applicability of the sediment transport and water flow characteristics-based simulation models in predicting downstream sediment transport and water flow rates in a river system. | Briti Sundar Sil Parthasarathi Choudhury | 2016 | International Journal of Sediment Research2016，31，2： | 2 |
| 14 | Studies of grand minima in sunspot cycles by using a flux transport solar dynamo model显示文摘We propose that grand minima in solar activity are caused by simultaneous fluctuations in the meridional circulation and the Babcock–Leighton mechanism for the poloidal field generation in the flux transport dynamo model.We present the following results:(a)fluctuations in the meridional circulation are more effective in producing grand minima;(b)both sudden and gradual initiations of grand minima are possible;(c)distributions of durations and waiting times between grand minima seem to be exponential;(d)the coherence time of the meridional circulation has an effect on the number and the average duration of grand minima,with a coherence time of about 30 yr being consistent with observational data.We also study the occurrence of grand maxima and find that the distributions of durations and waiting times between grand maxima are also exponential,like the grand minima.Finally we address the question of whether the Babcock–Leighton mechanism can be operative during grand minima when there are no sunspots.We show that anα-effect restricted to the upper portions of the convection zone can pull the dynamo out of the grand minima and can match various observational requirements if the amplitude of thisα-effect is suitably fine-tuned. | Bidya Binay Karak Arnab Rai Choudhuri | 2013 | Research in Astronomy and Astrophysics2013，13，11： | 2 |
| 15 | The arrival of commercial bioprinters-Towards 3D bioprinting revolution!显示文摘The dawn of commercial bioprinting is rapidly advancing the tissue engineering field.In the past few years,new bioprinting approaches as well as novel bioinks formulations have emerged,enabling biological research groups to demonstrate the use of such technology to fabricate functional and relevant tissue models.In recent years,several companies have launched bioprinters pushing for early adoption and democratisation of bioprinting.This article reviews the progress in commercial bioprinting since the inception,with a particular focus on the comparison of different available printing technologies and important features of the individual technologies as well as various existing applications.Various challenges and potential design considerations for next generations of bioprinters are also discussed. | Deepak Choudhury Shivesh Anand May Win Naing | 2018 | International Journal of Bioprinting2018，4，2： | 2 |
| 16 | Rituximab or plasmapheresis for prevention of recurrent focal segmental glomerulosclerosis after kidney transplantation:A systematic review and meta-analysis显示文摘BACKGROUND Focal segmental glomerulosclerosis(FSGS)is one of the most common glomerular diseases leading to renal failure.FSGS has a high risk of recurrence after kidney transplantation.Prevention of recurrent FSGS using rituximab and/or plasmapheresis has been evaluated in multiple small studies with conflicting results.AIM To assess the risk of recurrence of FSGS after transplantation using prophylactic rituximab with or without plasmapheresis,and plasmapheresis alone compared to the standard treatment group without preventive therapy.METHODS This meta-analysis and systematic review were performed by first conducting a literature search of the MEDLINE,EMBASE,and Cochrane databases,from inception through March 2021;search terms included‘FSGS,’’steroid-resistant nephrotic syndrome’,‘rituximab,’and‘plasmapheresis,’.We identified studies that assessed the risk of post-transplant FSGS after use of rituximab with or without plasmapheresis,or plasmapheresis alone.Inclusion criteria were:Original,published,randomized controlled trials or cohort studies(either prospective or retrospective),case-control,or cross-sectional studies;inclusion of odds ratio,relative risk,and standardized incidence ratio with 95%confidence intervals(CI),or sufficient raw data to calculate these ratios;and subjects without interventions(controls)being used as comparators in cohort and cross-sectional studies.Effect estimates from individual studies were extracted and combined using a random effects model.RESULTS Eleven studies,with a total of 399 kidney transplant recipients with FSGS,evaluated the use of rituximab with or without plasmapheresis;thirteen studies,with a total of 571 kidney transplant recipients with FSGS,evaluated plasmapheresis alone.Post-transplant FSGS recurred relatively early.There was no significant difference in recurrence between the group that received rituximab(with or without plasmapheresis)and the standard treatment group,with a pooled risk ratio of 0.82(95%CI:0.47-1.45,I2=65%).Similarly,plasmapheresis alone was not associated with any significant difference in FSGS recurrence when compared with no plasmapheresis;the pooled risk ratio was 0.85(95%CI:0.60-1.21,I2=23%).Subgroup analyses in the pediatric and adult groups did not yield a significant difference in recurrence risk.We also reviewed and analyzed posttransplant outcomes including timing of recurrence and graft survival.CONCLUSION Overall,the use of rituximab with or without plasmapheresis,or plasmapheresis alone,is not associated with a lower risk of FSGS recurrence after kidney transplantation.Future studies are required to assess the effectiveness of rituximab with or without plasmapheresis among specific patient subgroups with high-risk for FSGS recurrence. | Boonphiphop Boonpheng Panupong Hansrivijit Charat Thongprayoon Shennen A Mao Pradeep K Vaitla Tarun Bathini Avishek Choudhury Wisit Kaewput Michael A Mao Wisit Cheungpasitporn | 2021 | World Journal of Transplantation2021，11，7： | 2 |
| 17 | The hemispheric asymmetry of solar activity during the last century and the solar dynamo显示文摘We believe the Babcock-Leighton process of poloidal field generation to be the main source of irregularity in the solar cycle.The random nature of this process may make the poloidal field in one hemisphere stronger than that in the other hemisphere at the end of a cycle.We expect this to induce an asymmetry in the next sunspot cycle.We look for evidence of this in the observational data and then model it theoretically with our dynamo code.Since actual polar field measurements exist only from the 1970s,we use the polar faculae number data recorded by Sheeley(1991,2008) as a proxy of the polar field and estimate the hemispheric asymmetry of the polar field in different solar minima during the major part of the twentieth century.This asymmetry is found to have a reasonable correlation with the asymmetry of the next cycle.We then run our dynamo code by feeding information about this asymmetry at the successive minima and compare the results with observational data.We find that the theoretically computed asymmetries of different cycles compare favorably with the observational data,with the correlation co-efficient being 0.73.Due to the coupling between the two hemispheres,any hemispheric asymmetry tends to get attenuated with time.The hemispheric asymmetry of a cycle ei-ther from observational data or from theoretical calculations statistically tends to be less than the asymmetry in the polar field(as inferred from the faculae data) in the preceding minimum.This reduction factor turns out to be 0.43 and 0.51 respectively in observational data and theoretical simulations. | Ashish Goel Arnab Rai Choudhuri | 2009 | Chinese Journal of Astronomy and Astrophysics2009，9，1： | 2 |
| 18 | A general strategy based on the Newton-Euler approach for the dynamic formation of parallel manipulators显示文摘 | Dasgupta B Choudhury P | 1999 | Mechanism and Machine Theory1999，34，： | 2 |
| 19 | Yeast knockout library allows for efficient testing of genomic mutations for cell-free protein synthesis显示文摘Cell-free protein synthesis(CFPS)systems from crude lysates have benefitted from modifications to their enzyme composition.For example,functionally deleting enzymes in the source strain that are deleterious to CFPS can improve protein synthesis yields.However,making such modifications can take substantial time.As a proof-of-concept to accelerate prototyping capabilities,we assessed the feasibility of using the yeast knockout collection to identify negative effectors in a Saccharomyces cerevisiae CFPS platform.We analyzed extracts made from six deletion strains that targeted the single deletion of potentially negative effectors(e.g.,nucleases).We found a statistically significant increase in luciferase yields upon loss of function of GCN3,PEP4,PPT1,NGL3,and XRN1 with a maximum increase of over 6-fold as compared to the wild type.Our work has implications for yeast CFPS and for rapidly prototyping strains to enable cell-free synthetic biology applications. | Jennifer A.Schoborg Lauren G.Clark Alaksh Choudhury C.Eric Hodgman Michael C.Jewett | 2016 | Synthetic and Systems Biotechnology2016，1，1： | 2 |
| 20 | Soil texture classification using multi class support vector machine显示文摘The objective of this study is to process the soil images to generate a digital soil classification system for rural farmers at low cost.Soil texture is the main factor to be considered before doing cultivation.It affects the crop selection and regulates the water transmission property.The conventional hydrometer method determines the percentage of sand,silt,and clay present in a soil sample.This method is very cost and time-consuming process.In this approach,we collect 50 soil samples from the different region of west Guwahati,Assam,India.The samples are photographed under a constant light condition using an Android mobile of 13 MP cameras.The fraction of sand,silt,and clay of the soil samples are determined using the hydrometer test.The result of the hydrometer test is processed with the United State Department of Agriculture soil classification triangle for the final soil classification.Soil images are processed through the different stages like pre-processing of soil images for image enhancement,extracting the region of interest for segmentation and the texture analysis for feature vector.The feature vector is calculated from the Hue,Saturation,and Value(HSV)histogram,color moments,color auto Correlogram,Gabor wavelets,and discrete wavelet transform.Finally,Support Vector Machine classifier is used to classify the soil images using linear kernel.The proposed method gives an average of 91.37%accuracy for all the soil samples and the result is nearly the same with the United State Department of Agriculture soil classification. | Utpal Barman Ridip Dev Choudhury | 2020 | Information Processing in Agriculture2020，7，2： | 2 |
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