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| 1 | Microcalorimetric investigation of effect of berberine alkaloids from Coptis chinensis Franch on intestinal diagnostic flora growth显示文摘The inhibitory effect of three berberine alkaloids (BAs) from Coptis chinensis Franch, a traditional Chinese medicinal (TCM) herb, on Bifidobacterium adolescentis growth was investigated by micro- calorimetry. The power-time curves of B. adolescentis with and without BAs were acquired, meanwhile the extent and duration of inhibitory effect on the metabolism were evaluated by the growth rate con- stant (k), half inhibitory ratio (IC50), maximum heat-output power (Pmax), peak time of maximum heat-output power (tp) and total heat production (Qt). k, Pmax and Qt decreased, and tp was prolonged with the increase of BAs concentration. The IC50 of BAs is 806 μg/mL for berberine, 341 μg/mL for cop- tisine and 236 μg/mL for palmatine. The sequence of antimicrobial activity of BAs is berberine < cop- tisine < palmatine. Combined with previous studies, it could be shown that the sequences of antim- icrobial activity of BAs on both Bacillus shigae and Escherichia coli are berberine > coptisine > pal- matine. The structure-function relationship of BAs indicates that the functional group methylenedioxy or methoxyl at C2 and C3 might be the major group inducing the activities of BAs on E. coli and B. adolescentis. Meanwhile, the substituent groups at C2, C3, C9 and C10 almost have equal effect on B. shigae. | YAN Dan WEI Li XIAO XiaoHe ZHOU DanLei HAN YuMei | 2009 | Chinese Science Bulletin2009，54，3： | 12 |
| 2 | Added Value of Quantitative Apparent Diffusion Coefficients for Identifying Small Hepatocellular Carcinoma from Benign Nodule Categorized as LI-RADS 3 and 4 in Cirrhosis显示文摘Background and Aims:Correct identification of small hepa-tocellular carcinomas(HCCs)and benign nodules in cirrhosis remains challenging,quantitative apparent diffusion coeffi-cients(ADCs)have shown potential value in characterization of benign and malignant liver lesions.We aimed to explore the added value of ADCs in the identification of small(≤3 cm)HCCs and benign nodules categorized as Liver Imag-ing Reporting and Data System(LI-RADS)3(LR-3)and 4(LR-4)in cirrhosis.Methods:Ninety-seven cirrhosis patients with 109 small nodules(70 HCCs,39 benign nodules)of LR-3 and 4 LR-4 based on major and ancillary magnetic resonance imaging features were included.Multiparametric quantitative ADCs of the lesions,including the mean ADC(ADCmean),min-imum ADC(ADCmin),maximal ADC(ADCmax),ADC standard deviation(ADCstd),and mean ADC value ratio of lesion-to-liv-er parenchyma(ADCratio)were calculated.Regarding the joint diagnosis,a nomogram model was plotted using multivariate logistic regression analysis.The performance was assessed using the area under the receiver operating characteristic curve(AUC).Results:The ADCmean,ADCmin,ADCratio,and ADCstd were significantly associated with the identification of small HCC and benign nodules(p<0.001).For the joint diagnosis,the LI-RADS category(odds ratio[OR]=12.50),ADCmin(OR=0.14),and ADCratio(OR=0.12)were identified as independent factors for distinguishing HCCs from benign nodules.The joint nomogram model showed good calibration and discrimination,with a C-index of 0.947.Compared with the LI-RADS category alone,this nomogram model demon-strated a significant improvement in diagnostic performance,with AUC increasing from 0.820 to 0.967(p=0.001).Con-clusions:The addition of quantitative ADCs could improve the identification of small HCC and benign nodules catego-rized as LR-3 and 4 LR-4 in patients with cirrhosis. | Xi Zhong Hongsheng Tang Tianpei Guan Bingui Lu Chuangjia Zhang Danlei Tang Jiansheng Li Shuzhong Cui | 2022 | Journal of Clinical and Translational Hepatology2022，10，1： | 6 |
| 3 | Pathological features of COVID-19-associated lung injury:a preliminary proteomics report based on clinical samples显示文摘The COVID-19 pandemic has emerged as a global health emergency due to its association with severe pneumonia and relative high mortality.However,the molecular characteristics and pathological features underlying COVID-19 pneumonia remain largely unknown.To characterize molecular mechanisms underlying COVID-19 pathogenesis in the lung tissue using a proteomic approach,fresh lung tissues were obtained from newly deceased patients with COVID-19 pneumonia.After virus inactivation,a quantitative proteomic approach combined with bioinformatics analysis was used to detect proteomic changes in the SARS-CoV-2-infected lung tissues.We identified significant differentially expressed proteins involved in a variety of fundamental biological processes including cellular metabolism,blood coagulation,immune response,angiogenesis,and cell microenvironment regulation.Several inflammatory factors were upregulated,which was possibly caused by the activation of NF-κB signaling.Extensive dysregulation of the lung proteome in response to SARS-CoV-2 infection was discovered.Our results systematically outlined the molecular pathological features in terms of the lung response to SARS-CoV-2 infection,and provided the scientific basis for the therapeutic target that is urgently needed to control the COVID-19 pandemic. | Ling Leng Ruiyuan Cao Jie Ma Danlei Mou Yunping Zhu Wei Li Luye Lv Dunqin Gao Shikun Zhang Feng Gong Lei Zhao Bintao Qiu Haiping Xiang Zhongjie Hu Yingmei Feng Yan Dai Jiang Zhao Zhihong Wu Hongjun Li Wu Zhong | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 4 |
| 4 | Effects of Temperature and pH on the Physiological Activity of Grape Juice显示文摘This study was conducted to investigate the effects of temperature and pH on polyphenol and flavonoid contents in grape juice and its antioxidant activity. Two groups(group A and group B) were set. Group A was set with different pH values: 3.0, 3.5, 4.0, 4.5 and 5.0; and group B was set with different temperatures: 65, 72, 79, 86 and 93 ℃. The grape juice was treated according to set methods, and two groups of samples were obtained. The contents of polyphenols and flavonoids in each test solution were determined, and the DPPH·free radical system and trivalent Fe reduction system were established to measure and compare the antioxidant activity of groups A and B. The results showed that within the set ranges, the effects of pH and temperature on the content of flavonoids in grape juice were not significant; the test solution with a pH of 4.5 and the test solution with a temperature at 72 ℃ had the strongest capacities of scavenging free radicals, and exhibited scavenging rates of 6.82% and 9.43%, respectively; the test solution with a pH of 4.5 and the test solution with a temperature at 65 ℃ had the strongest capacities of reducing ferric ions. It could be seen from the above four aspects comprehensively, within the set test ranges, the suitable pH was 4.5, and the suitable temperature was 65 ℃. | Danlei LI Chunling XIAO | 2019 | Agricultural Biotechnology2019，8，2： | 2 |
| 5 | Development of an In-House Multiplex Nested RT-PCR Method for Detecting Acute HIV-1 Infection in High Risk Populations显示文摘 | Zhiying Liu Wei Li Meng Xu Bo Sheng Zixuan Yang Yanmei Jiao Tong Zhang Danlei Mou Dexi Chen Hao Wu | 2015 | Current HIV Research2015，，： | 1 |
| 6 | Identification and characterization of a Bursaphelenchus xylophilus ( Aphelenchida: Aphelenchoididae) thermotolerance-related Gene: Bx-HSP90显示文摘 | Wang Feng Wang Zhiying Li Danlei | 2012 | Int J Mol Sei2012，13，7： | 1 |
| 7 | Process and Component Analysis on S-CO_(2)Cooling Wall in the Coal-fired Boiler Power System显示文摘The supercritical carbon dioxide(S-CO_(2))cooling wall in coal-fired boiler suffers from severe fragile crisis due to the high temperature of S-CO_(2).The analysis of both heat transfer at process scale and cooling wall arrangement at component scale were carried out in present work.At the process scale,the difference in heat transfer performance between the smooth tube and the rifled tube were identified,especially the location of maximum outer wall temperature of cooling wall.The 1-D mathematical model for thermal-hydraulic analysis of S-CO_(2)furnace cooling wall tubes was then developed.At the component scale,the coupled model of combustion and S-CO_(2)heat transfer is employed for studying the thermal-hydraulic performance of rifled-spiral(R-S)and smooth-spiral(S-S)cooling wall arrangements.The maximum outer wall temperature of R-S cooling wall is 16.38℃lower while the pressure drop increases by 2.33 times compared with the S-S cooling wall.Considering the pressure drop penalty on cycle efficiency of S-CO_(2)boiler power system,the R-S cooling wall is not recommended,while the S-S cooling wall should be carefully arranged in S-CO_(2)boilers. | Yuanhong FAN Danlei YANG Guihua TANG Xiaolong LI | 2021 | Mechanical Engineering Science2021，3，2： | 0 |
| 8 | Pancreatic head cancer with gastrointestinal bleeding as the initial manifestation:a case report and literature review显示文摘Gastrointestinal bleeding(GIB)as the initial symptom is rare in patients with pancreatic cancer,which is prone to misdiagnosis and should be paid attention to by clinicians.Here,we present a patient with pancreatic head cancer whose first symptom was only severe upper gastrointestinal bleeding,which initially led to misdiagnosis.However,subsequent imaging revealed a mass in the pancreatic head which was considered a malignant tumor.After surgical resection,the pathology confirmed that the mass in the pancreatic head was pancreatic ductal adenocarcinoma,and the duodenal bulb’s full thickness was infiltrated.A literature review found that pancreatic cancer can present gastrointestinal bleeding by invading the digestive tract and blood vessels,left-sided portal hypertension(LSPH),digestive tract metastasis,and canceration of ectopic pancreatic tissue in the digestive tract.For these cases,clinicians should utilize multidisciplinary diagnosis and treatment based on digestive endoscopy,imaging,interventional therapy,and surgery to control bleeding,identify etiology,and remove tumors. | Minghui Zheng Zhiping Fu Ting Niu Anan Liu Danlei Chen Junfeng Peng Judong Li Zhiyuan Hao Qingyang Yu Zechao Lyu Chenghao Shao | 2023 | Journal of Pancreatology2023，6，3： | 0 |
| 9 | In vitro inhibition of HIV-1 replication in autologous CD4*T cells indicates viral containment by multifactorial mechanisms显示文摘HIV-1-specific cytotoxic T lymphocytes(CTLs) and neutralizing antibodies(NAbs) are present during chronic infection, but the relative contributions of these effector mechanisms to viral containment remain unclear. Here, using an in vitro model involving autologous CD4+ T cells,primary HIV-1 isolates, HIV-1-specific CTLs, and neutralizing monoclonal antibodies, we show that b12, a potent and broadly neutralizing monoclonal antibody to HIV-1, was able to block viral infection when preincubated with virus prior to infection, but was much less effective than CTLs at limiting virus replication when added to infected cell cultures. However, the same neutralizing antibody was able to contain viruses by antibody-dependent cell-mediated virus inhibition in vitro,which was mediated by natural killer cells(NKs) and dependent on an Fc-Fc receptor interaction.Meanwhile, bulk CTLs from HIV-1 controllers were more effective in suppression of virus replication than those from progressors. These findings indicate that control of HIV-1 replication in activated CD4^+ T cells is ineffectively mediated by neutralizing antibodies alone, but that both CTLs and antibody-dependent NK-mediated immune mechanisms contribute to viral containment. Our study systemically compared three major players in controlling HIV-1 infection, CTLs, NAbs, and NKs, in an autologous system and highlighted the multifactorial mechanisms for viral containment and vaccine success. | Ting Tu Jianbo Zhan Danlei Mou Wei Li Bin Su Tong Zhang Tao Li Ning Li Hao Wu Cong Jin Huabiao Chen | 2017 | Virologica Sinica2017，32，6： | 0 |
| 10 | Traditional Chinese medicine for promoting mental health of patients with COVID-19:a scoping review显示文摘Objective:This study aimed to systematically review and depict the current studies of traditional Chinese medicine for the mental health of patients with coronavirus disease 2019(COVID-19).Methods:A scoping review was conducted by searching PubMed,Web of Science,CNKI,Wanfang database,VIP database,and SinoMed,with the retrieval time being from the establishment of the database to April 18,2022.The basic information of the included studies,objective,design,types of patients,interventions,outcomes,etc.,was reviewed and summarized narratively.Methodological quality was assessed using the Cochrane Risk of Bias assessment tool,the methodological index for nonrandomized studies or the Newcastle-Ottawa scale.Results:We identified 30 traditional Chinese medicine(TCM)studies from six databases.Among them,finished randomized controlled trials(n=16)accounted for most of the studies,followed by single-arm studies(n=9).In terms of study theme,20 studies defined the mental health of patients with COVID-19 as the research theme.Psychological assessment was included in the inclusion criteria(performed before participation)of nine studies,whereas the other studies only mentioned the mental outcomes.TCM interventions included TCM exercises(Yijinjing,Baduanjin,Liuzijue,Taichi),acupoint stimulation(auricular and body points),moxibustion,decoction,or granules based on TCM syndrome differentiation,decoction,or granules with fixed formulae(Baidu Jieduan granules,Xuanfei Baidu decoction,and Qingfei Paidu decoction),Chinese patent medicine(Jinhua Qinggan granules),TCM psychological therapy(TCM ideological therapy,TCM five-tone therapy,and TCM psychological sand table),and TCM nursing(dialectical care,dialectical diet,and psychological counseling).Anxiety and depression were the main outcomes evaluated in regard to mental health in patients with COVID-19.The limitations of methodological quality were predominantly from follow-up,blinding,and registration.Positive results were reported by 27 studies(90%,n=30).Conclusion:We summarized the existing literature about the impact of TCM on mental health in patients with COVID-19.The number of studies evaluating the impact of TCM on mental health is encouraging,but overall methodological quality was low.Several TCM interventions warrant further evaluation,particularly among populations outside of China,for the purpose of establishing supporting evidence.More importantly,research with stronger methodological quality needs to be developed. | Zhaochen Ji Haiyin Hu Danlei Wang Marco Di Nitto Alice Josephine Fauci Masayoshi Okada Kai Li Hui Wang | 2022 | Acupuncture and Herbal Medicine2022，2，3： | 0 |
| 11 | Full-Length Transcriptome Analysis of Cultivated and Wild Tetraploid Peanut显示文摘The high-quality genomes and large-scale full-length cDNA sequences of allotetraploid peanuts have been sequenced and released,which has accelerated the functional genomics and molecular breeding research of peanut.In order to understand the difference in the transcriptional levels of wild and cultivated peanuts.In this study,we integrated of second-and third-generation sequencing technologies to sequence full-length transcriptomes in peanut cv.Pingdu9616 and its putative ancestor Arachis monticola.The RNA extracted from six different tissues(i.e.,roots,stems,leaves,flowers,needles and pods)were sampled at 20 days after flowering.A total of 31,764 and 33,981 high-quality transcripts were obtained from Monticola and Pingdu9616,respectively.The number of alternative splicing,the unit point mutation of variable adenylation,the number of open reading frames and the two-site mutation were identified in Pingdu9616 more than in Monticola,but the three-site mutation in Pingdu9616 was lower than in Monticola.1,691 LncRNAs,and 4,000 bp of maximum length of LncRNA was identified in Monticola and Pingdu9616.Furthermore,comparative analysis between transcript data shown that 56 transcription factor families were involved in Monticola,and Pingdu9616 and the number of transcription factors in Pingdu9616 was higher than that in Monticola,the number of expressed genes estimated in flower,root,young pod and leaf organs was higher in Monticola than Pingdu9616.Over all,our study provided a valuable resource of large-scale full-length transcripts for further research of the molecular breeding and functional analysis of genes. | Danlei Song Xiaona Yu Yaoyao Li Xianheng Wang Xinyuan Cui Tong Si Xiaoxia Zou Yuefu Wang Minglun Wang Xiaojun Zhang | 2023 | Phyton-International Journal of Experimental Botany2023，92，2： | 0 |
| 12 | Solution-processed top-contact electrodes strategy for organic crystalline field-effect transistor arrays显示文摘Organic crystals,especially ultra-thin two-dimensional(2D)ones such as monolayer molecular crystals,are fragile and vulnerable to traditional vacuum deposition.Up to now,most of the methods reported for fabricating organic field-effect transistors(OFETs)with top-electrodes on the 2D molecular crystals are based on mechanical-transfer method.Nondestructive method for large scale in-situ electrode deposition is urgent.In this work,the silver mirror reaction(SMR)is introduced to construct top-contact electrodes on 2D organic crystalline thin films.OFETs based on bilayer crystalline films with solution-processed silver electrodes show comparable performance to devices with transferred gold electrodes.In addition to that,OFETs with SMR fabricated silver electrodes show lower contact resistance than the ones with evaporated silver electrodes.Furthermore,the temperature under which SMR electrodes annealed is relatively low(60℃),making this approach applicable to varies of organic semiconductors,such as spin-coated polymer films,vacuum evaporated films,2D and even monolayer crystalline films.Besides,OFETs with sub-micrometer channel width and 25μm channel length are realized which might find practical application in the ultra-small pixel mini/micro-LEDs. | Xi Zhang Xiaotong Zhao Limei Rao Jing Zhang Mingchao Xiao Danlei Zhu Chunlei Li Xiaosong Shi Jie Liu Jie Liu Lang Jiang | 2022 | Nano Research2022，15，2： | 0 |
| 13 | Sera proteomic features of active and recovered COVID-19 patients:potential diagnostic and prognostic biomarkers显示文摘Dear Editor,The early diagnosis and prognosis of COVID-19 remain major challenges.At present,there are few studies focusing on the changes of sera proteome betAA/een active and recovered COVID-19 patients,and almost no biomarkers in sera are used to predict the function disorder of the injured tissues and organs in COVID-19 patients.In this study,we applied sera proteomics in the active and recovered COVID-19 patients,as well as healthy volunteers,combining with pathological staining of the patients'tissues to identify the potential prognosis biomarkers that can reflect specific organ damage. | Ling Leng Mansheng Li Wei Li Danlei Mou Guopeng Liu Jie Ma Shuyang Zhang Hongjun Li Ruiyuan Cao Wu Zhong | 2021 | Signal Transduction and Targeted Therapy2021，6，7： | 0 |
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