|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Variation in cis-regulation of a NAC transcription factor contributes to drought tolerance in wheat显示文摘Drought is a major environmental factor limiting wheat production worldwide,and developing drought-tolerant cultivars is a central challenge for wheat breeders globally.Therefore,it is important to identify genetic components determining drought tolerance in wheat.In this study,we identified a wheat NAC gene(TaNAC071-A)that is tightly associated with drought tolerance by a genome-wide association study.Knockdown of TaNAC071-A in wheat attenuated plant drought tolerance,whereas its overexpression significantly enhanced drought tolerance through improved water-use efficiency and increased expression of stress-responsive genes.This heightened water-saving mechanism mitigated the yield loss caused by water deficit.Further candidate gene association analysis showed that a 108-bp insertion in the promoter of TaNAC071-A alters its expression level and contributes to variation in drought tolerance among wheat accessions.This insertion contains two MYB cis-regulatory elements(CREs)that can be directly bound by the MYB transcription activator,TaMYBL1,thereby leading to increased TaNAC071-A expression and plant drought tolerance.Importantly,introgression of this 108-bp insertion allele,TaNAC071-AIn-693,into drought-sensitive cultivars could improve their drought tolerance,demonstrating that it is a valuable genetic resource for wheat breeding.Taken together,our findings highlight a major breakthrough in determining the genetic basis underlying phenotypic variation in wheat drought tolerance and showcase the potential of exploiting CRE-containing indels for improving important agronomical traits. | Hude Mao Shumin Li Bin Chen Chao Jian Fangming Mei Yifang Zhang Fangfang Li Nan Chen Tian Li Linying Du Li Ding Zhongxue Wang Xinxiu Cheng Xiaojing Wang Zhensheng Kang | 2022 | Molecular Plant2022，15，2： | 9 |
| 2 | Plant Programmed Cell Death Caused by an Autoactive Form of Prf Is Suppressed by Co-Expression of the Prf LRR Domain显示文摘在西红柿, NBARC-LRR 抵抗(R) 蛋白质 Prf 与 Pto 或分 kinase 在音乐会行动对 Pseudomonas syringae pv 决定免疫。西红柿(Pst ) 。发信号的调停 Prf 的防卫被二顺序无关的藏匿 Pst 的受动器蛋白质, AvrPto 和 AvrPtoB 的识别开始,由西红柿 Pto 或分。Prf 检测这些相互作用并且激活发信号包括与 Pst 生长的拘捕被联系的局部性的规划细胞死亡(PCD ) 导致宿主防卫回答。当短暂地在 Nicotiana benthamiana 的叶子表示了时,我们发现有在在在 NBARC 领域的 IHD 主题(isoleucine-histidine-aspartic 酸) 的 D1416 的单个氨基酸替换的 Prf 变体引起受动器无关的 PCD,建议 D1416 起在 Prf 的激活的一个重要作用。Prf (NPrf ) 和 LRR 领域的 N 终端区域为发信号的这 autoactive Prf 房间死亡被要求但是为 PrfD1416V 蛋白质的累积非必需。显著地, Prf LRR 然而并非 NPrf 的合作表示与 PrfD1416V, AvrPto/Pto, AvrPtoB/Pto, Pto (PtoY207D ) 的一种 autoactive 形式,或分完全压制 PCD。然而, Prf LRR 不防碍 PCD 由 Rpi-blb1D475V 引起了,表明事件的不同 R 调停蛋白质的 PCD,或由 MAPKKK 的 overexpression 引起了, Prf 下游地行动的蛋白质。而且,我们发现当与 Prf LRR 领域共同表示时, PrfD1416V 蛋白质是不能的在植物房间积累,多半向房间死亡解释抑制。为 PrfD1416V 的导致 LRR 的降级的机制是未知的,但是可以在 Prf 或到独立 LRR 的绑定的 intramolecular 相互作用包含干扰到为 Prf 稳定性被需要的另外的主机蛋白质。 | Xinran Du Min Miao Xinrong Ma Yongsheng Liu Joseph C. Kuhl Gregory B. Martin Fangming Xiao | 2012 | Molecular Plant2012，5，5： | 4 |
| 3 | Nanotube Networks in Polymer Nanocomposites: Rheology and Electrical Conductivity显示文摘 | Du Fangming Scogna R C Zhou W | 2004 | Macromolecules2004，37，24： | 1 |
| 4 | Nanotube networks in polymer nanocomposites:rbeology and electrical conductivity显示文摘 | FANGMING DU ROBERT C SCOGNA | 2004 | Macromolecules2004，37，24： | 1 |
| 5 | Flamma bility Properties of Polymer Nanocomposites with Single- walled Carbon Nanotubes: Effects of Nanotube Dispersion and Concentration显示文摘 | Kashiwagi T Fangming Du F Winey I K | 2005 | Polymer2005，46，2： | 1 |
| 6 | 显示文摘 | Du C FangM Li Y etal | 2000 | Cell2000，102，6： | 1 |
| 7 | Alkaline pretreatment of rice hulls for hydrothermal production of acetic aeid显示文摘 | HSIEH Y DU Yingxun JIN Fangming | 2009 | Chemical Engineering Research and Design2009，87，1： | 1 |
| 8 | Nanopartiele networks reduce the flammability of polymer nanocomposites显示文摘 | Kashiwagi T Du Fangming M Douglas J F | 2005 | Nature Materials2005，4，12： | 1 |
| 9 | Nanotube Networks in Polymer Nanocomposites:Rheology and Electrical Conductivity显示文摘 | Du Fangming Scogna R C Zhou Wei | 2004 | Macromolecules2004，37，24： | 1 |
| 10 | Induction of a tumour-specific CTL response by exosomes isolated from heat-treated malignant ascites of gastric cancer patients显示文摘 | Haijun Zhong Yunshan Yang Shenglin Ma Fangming Xiu Zhijian Cai Hongguang Zhao Linbin Du | 2011 | International Journal of Hyperthermia2011，，6： | 1 |
| 11 | Mediator complex subunit MED25 physically interacts with DST to regulate spikelet number in rice显示文摘Grain number is a flexible trait and contributes significantly to grain yield.In rice,the zinc finger transcription factor DROUGHT AND SALT TOLERANCE(DST)controls grain number by directly regulating cytokinin oxidase!dehydrogenase 2(OsCKX2)expression.Although specific upstream regulators of the DST-OsCKX2 module have been identified,the mechanism employed by DST to regulate the expression of OsCKX2 remains unclear.Here,we demonstrate that DST-interacting protein 1(DIP1),known as Mediator subunit OsMED25,acts as an interacting coactivator of DST.Phenotypic analyses revealed that OsMED25-RNAi and the osmed25 mutant plants exhibited enlarged panicles,with enhanced branching and spikelet number,similar to the dst mutant.Genetic analysis indicated that OsMED25 acts in the same pathway as the DST-OsCKX2 module to regulate spikelet number per panicle.Further biochemical analysis showed that OsMED25 physically interacts with DST at the promoter region of OsCKX2,and then recruits RNA polymerase II(Pol II)to activate OsCKX2 transcription.Thus,OsMED25 was involved in the communication between DST and Pol II general transcriptional machinery to regulate spikelet number.In general,our findings reveal a novel function of OsMED25 in DST-OsCKX2 modulated transcriptional regulation,thus enhancing our un derstanding of the regulatory mechanism underlying DST-OsCKX2-mediated spikelet number. | Lihao Lin Minmin Du Shuyu Li Chuanlong Sun Fangming Wu Lei Deng Qian Chen Chuanyou Li | 2022 | Journal of Integrative Plant Biology2022，64，4： | 1 |
| 12 | Effect of component and microstrueture on impact wear property and mechanism of steels in corrosive condition 显示文摘 | DING Houfu CUI Fangming DU Xiaodong | 2006 | Materials Science and Engineering: A2006，421，12： | 1 |
| 13 | Smac, a mitochondrial protein that pro-motescytochrome cdependent caspase activation by eliminating IAP in-hibition显示文摘 | Du CY FangM Li YC | 2000 | Cell2000，102，1： | 1 |
| 14 | Coagulation Method for Preparing Single-walled Carbon Nanotube/ Poly(methyl methacrylate) Composites and Their Modu- lus, Electrical Conductivity, and Thermal Stability显示文摘 | Du Fangming Fischer J E Winey K I | 2003 | Journal of Polymer Science Part B: Polymer Physics2003，41，24： | 1 |
| 15 | The enhanced genomic 6mA metabolism contributes to the proliferation and migration of TSCC cells显示文摘In contrast to the well-established genomic 5-methylcytosine(5mC),the existence of N^(6)-methyladenine(6 mA)in eukaryotic genomes was discovered only recently.Initial studies found that it was actively regulated in cancer cells,suggesting its involvement in the process of carcinogenesis.However,the contribution of 6 mA in tongue squamous cell carcinoma(TSCC)still remains uncharacterized.In this study,a pan-cancer type analysis was first performed,which revealed enhanced 6mA metabolism in diverse cancer types.The study was then focused on the regulation of 6 mA metabolism,as well as its effects on TSCC cells.To these aspects,genome 6mA level was found greatly increased in TSCC tissues and cultured cells.By knocking down 6mA methylases N6AMT1 and METTL4,the level of genomic 6mA was decreased in TSCC cells.This led to suppressed colony formation and cell migration.By contrast,knockdown of 6mA demethylase ALKBH1 resulted in an increased 6 mA level,enhanced colony formation,and cell migration.Further study suggested that regulation of the NF-κB pathway might contribute to the enhanced migration of TSCC cells.Therefore,in the case of TSCC,we have shown that genomic 6mA modification is involved in the proliferation and migration of cancer cells. | Lei Xi Ying Yang Ying Xu Fangming Zhang Jinghui Li Xiyang Liu Zhenxi Zhang Quan Du | 2022 | International Journal of Oral Science2022，14，1： | 0 |
| 16 | ISAR Imaging and Cross-Range Scaling Based on Image Rotation Correlation显示文摘With the rapid advancement of technology,not only do we need to acquire a clear in-verse synthetic aperture radar(ISAR)image,but also the real size of the target on the imaging plane,so it’s particularly important for the ISAR to rescale the images.That is,the ISAR image which is in the range-Doppler domain is converted into the range-azimuth domain.Actually,the key point to solving the problem is to estimate the rotation parameters.In this paper,a new scheme to rescale the images is proposed.For the sake of solving the problem of two-dimensional image blur and target high-speed,the instantaneous range instantaneous Doppler(IRID)method is used to obtain ISAR images,and the rotation parameters are estimated by comparing the rotation correlation of the two images.Using this method,the error of the estimated rotation parameters is greatly reduced,so that the target can be rescaled accurately.The simulation results verify the ef-fectiveness of the proposed algorithm. | Fangming Liu Cong Du Yujiao Ding Minghai Wang Wei Dong | 2022 | Journal of Beijing Institute of Technology2022，31，2： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员