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| 1 | Enhancing disease resistances of Super Hybrid Rice with four antifungal genes显示文摘A plant expression vector harboring four antifungal genes was delivered into the embryogenic calli of ‘9311’, an indica restorer line of Super Hybrid Rice, via modified biolistic particle bombardment. Southern blot analysis indicated that in the regenerated hygromycin-resistant plants, all the four anti-fungal genes, including RCH10, RAC22, β-Glu and B-RIP, were integrated into the genome of ‘9311’, co-transmitted altogether with the marker gene hpt in a Mendelian pattern. Some transgenic R1 and R2 progenies, with all transgenes displaying a normal expression level in the Northern blot analysis, showed high resistance to Magnaporthe grisea when tested in the typical blast nurseries located in Yanxi and Sanya respectively. Furthermore, transgenic F1 plants, resulting from a cross of R2 homo-zygous lines with high resistance to rice blast with the non-transgenic male sterile line Peiai 64S, showed not only high resistance to M. grisea but also enhanced resistance to rice false smut (a disease caused by Ustilaginoidea virens) and rice kernel smut (another disease caused by Tilletia barclayana). | ZHU HuaChen1, XU XinPing1, XIAO GuoYing2,3, YUAN LongPing2 & LI BaoJian1 1 Biotechnology Research Center, Key Laboratory of Gene Engineering of Ministry of Education, Sun Yat-sen University, Guangzhou 510275, China 2 China National Hybrid Rice R&D Center, Hunan Hybrid Rice Research Center, Changsha 410125, China 3 Institute of Subtropical Agriculture, the Chinese Academy of Sciences, Changsha 410125, China | 2007 | Science China(Life Sciences)2007，50，1： | 6 |
| 2 | Macrophage secretory products induce an inflammatory phenotype in hepatocytes显示文摘AIM:To investigate the influence of macrophages on hepatocyte phenotype and function.METHODS:Macrophages were differentiated from THP-1 monocytes via phorbol myristate acetate stimulation and the effects of monocyte or macrophageconditioned medium on HepG2 mRNA and protein expression determined.The in vivo relevance of these findings was confirmed using liver biopsies from 147 patients with hepatitis C virus(HCV)infection.RESULTS:Conditioned media from macrophages,but not monocytes,induced a transient morphological change in hepatocytes associated with upregulation of vimentin(7.8±2.5-fold,P=0.045)and transforming growth factor(TGF)-β1(2.6±0.2-fold,P<0.001)and downregulation of epithelial cadherin(1.7±0.02-fold,P=0.017)mRNA expression.Microarray analysis revealed significant upregulation of lipocalin-2(17-fold,P <0.001)and pathways associated with inflammation,and substantial downregulation of pathways related to hepatocyte function.In patients with chronic HCV,realtime polymerase chain reaction and immunohistochemistry confirmed an increase in lipocalin-2 mRNA(F0 1.0 ±0.3,F1 2.2±0.2,F2 3.0±9.3,F3/4 4.0±0.8,P= 0.003)and protein expression(F1 1.0±0.5,F2 1.3± 0.4,F3/4 3.6±0.4,P=0.014)with increasing liver injury.High performance liquid chromatography-tandem mass spectrometry analysis identified elevated levels of matrix metalloproteinase(MMP)-9 in macrophageconditioned medium,and a chemical inhibitor of MMP-9 attenuated the change in morphology and mRNA expression of TGF-β1(2.9±0.2 vs 1.04±0.1,P<0.001) in macrophage-conditioned media treated HepG2 cells.In patients with chronic HCV infection,hepatic mRNA expression of CD163(F0 1.0±0.2,F1/2 2.8±0.3,F3/4 5.3±1.0,P=0.001)and MMP-9(F0 1.0±0.4,F1/2 2.8±0.3,F3/4 4.1±0.8,P=0.011)was significantly associated with increasing stage of fibrosis.CONCLUSION:Secreted macrophage products alter the phenotype and function of hepatocytes,with increased expression of inflammatory mediators,suggesting that hepatocytes actively participate in liver injury. | Michelle Melino Victoria L Gadd Gene V Walker Richard Skoien Helen D Barrie Dinesh Jothimani Leigh Horsfall Alun Jones Matthew J Sweet Gethin P Thomas Andrew D Clouston Julie R Jonsson Elizabeth E Powell | 2012 | World Journal of Gastroenterology2012，18，15： | 3 |
| 3 | Block adjustment of high- resolution satellite images described by rational polynorials 显示文摘 | Gene D | 2003 | PERS2003，69，1： | 1 |
| 4 | 显示文摘 | Miyamoto K-I Taketani Y A Vitamin D receptor gene polymorphism in the translation initiation codon: Effect on protein activity and ralation to bone mineral density in Japanese woman | 1997 | J Bone Miner Res1997，12，： | 1 |
| 5 | 显示文摘 | Eccleshall TR Malloy PJ The presence of a polymor -phism at the translation initiationsite of the vitamin D receptor gene is associated with low bone mineral density in postmenopausal Mexican- American women | 1996 | J Bone Miner Res1996，11，： | 1 |
| 6 | proteins 显示文摘 | Habets GG Scholtes EH Zuydgeest D Indentification of an invasion-inducing gene Tiaml that encodes a protein with homology to GDP-GTP exchangers for Rho-like | 1994 | Cell1994，77，4： | 1 |
| 7 | Guidelines for the prevention of falls in people over 65显示文摘 | Gene F Colin C Sheila D | 2000 | BMJ2000，321，7267： | 1 |
| 8 | coliβ-galactosidase显示文摘 | Farquhar D Pan BF Sakurai M Suicide gene therapy usingE | 2002 | Cancer Chemother Pharmacol2002，50，： | 1 |
| 9 | A comparative clinical study of short-term results of laparoscopic surgery for rectal cancer during the learning curve显示文摘 | Ivan Arteaga González Hermógenes Díaz Luis Antonio Martín Malagón Eudaldo M. López-Tomassetti Fernández Javier Arranz Duran ángel Carrillo Pallares | 2006 | International Journal of Colorectal Disease2006，，6： | 1 |
| 10 | Science requirementand design of cabled ocean observatories显示文摘 | Alan D Chave Gene Massion Hitoshi Mikada | 2006 | ANNALS OF EO-PHYSICS2006，49，23： | 1 |
| 11 | Physical restraint use in Turk-ish intensive care units显示文摘 | Turgay AS Sari D Gene RE | 2009 | Clin Nurse Spec2009，23，2： | 1 |
| 12 | Rheology of high internal phase emulsions显示文摘 | Cynthia F Welch Gene D | 2006 | Langmuir2006，22，： | 1 |
| 13 | strai PYR-1 显示文摘 | Khan AA Wang RF Cao WW Doerge DR Wennerstrom D Cerniglia CE Molecular cloning nucleotide sequence and expression of genes encoding a polycyclic aromatic ring dioxygenase from Mycobacterium sp | 2001 | Appl Environ Microbiol2001，67，： | 1 |
| 14 | Geographical factors and China's regional development under market reform, 1979-1998显示文摘 | Bao Shu-ming Chang Gene Hsin Sachs Jeffery D | 2002 | China Economic Review2002，13，1： | 1 |
| 15 | Laparoscopic Heller Myotomy and Fundoplication for Achalasia 显示文摘 | Jonh G Thadeus L Gene D | 1997 | Annals of Surgery1997，225，6： | 1 |
| 16 | A genome-wide linkage and association scan reveals novel loci for autism 显示文摘 | Weiss L A Arking D E Gene Discovery Project of Johns Hopkins & the Autism Consortium | 2009 | Nature2009，461，7265： | 1 |
| 17 | A risk factor for coronary heart disase and myocardial infarction 显示文摘 | Jiang H Klein RM Niederacher D C/T polymorphism of the intercellular adhesion molecule - 1 gene (exon 6 codon 469) | 2002 | Int J Cardiol2002，84，23： | 1 |
| 18 | Marginal fit of castable ceramic crowns显示文摘 | J Robert Holmes DDS MS MED William D Sulik DDS MS Gene A Holland DDS MS | 1992 | J Prosthetic Dent1992，67，3： | 1 |
| 19 | Beta-catenin novel metalloproteinase expressed both in carcinomas and normal epithelial cells显示文摘 | Marchenko N D Marchenko G N regulates the gene of MMP-26 a Weinreb R N | 2004 | Int J Biochem Cell Biol2004，36，5： | 1 |
| 20 | Geographic factors and China s regional development under market re forms,1978-1998显示文摘 | Shuming Bao Gene Hsin Chang Jeffrey D | 2002 | China Economic Review2002，13，1： | 1 |
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