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| 1 | Contribution of public places in proliferation of dengue vectors in Penang Island,Malaysia显示文摘Objective: To determine abundance, distribution and diversity of potential breeding container habitats of the dengue vectors in public places including schools, restaurants,mosques and parks in southwest areas of Penang Island, Malaysia.Methods: Premises at restaurants, schools, parks and mosques were surveyed simultaneously and inspected visually for container habitats and production of immature mosquitoes from March 2015 to March 2016. Abundance(mean ± SE) of breeding containers between sites was compared using One-way ANOVA. Independent sample ttest was used to compare total number of Aedes albopictus(Ae. albopictus) and Aedes aegypti(Ae. aegypti) surveyed.Results: The surveyed locations yielded a total of 3 741 breeding containers and 19 537 immature mosquitoes from four areas. Concurrent artificial and natural containers produced 78.4% immature Ae. albopictus and 6.3% Ae. aegypti mosquitoes in wet season,with 14.2% Ae. albopictus and 1.1% Ae. aegypti mosquitoes in dry season. Artificial containers accounted for 98.1% of the total containers recorded, with restaurants being the most productive locations(8 012) and schools being the least productive(2 234).Conclusions: It was concluded that public places are good sources of potential container habitats of Aedes mosquitoes in Penang Island, Malaysia and Ae. albopictus has exclusively replaced the home-grown Ae. aegypti even in urban areas. Therefore, treatment of artificial containers in such locations is critical in Aedes mosquito control campaigns during dengue outbreaks. | Alhaji Hamisu Maimusa Abu Hassan Ahmad Nur Faeza Abu Kassim Hamdan Ahmad Hamady Dieng Junaid Rahim | 2017 | Asian Pacific Journal of Tropical Biomedicine2017，7，3： | 2 |
| 2 | The biology and demographic parameters of Aedes albopictus in northern peninsular Malaysia显示文摘Objective:To generate life table characteristics for the dengue vector Aedes albopictus(A. albopictus) under uncontrolled conditions,incorporating both the aquatic and the adult stages. Methods:Ten females derived from wild pupae were allowed to fully blood-feed on restrained mice.774 eggs were hatched in seasoned water.F1 larvae were followed for development until their F2 counterparts emerged as adults.Some population parameters were monitored(Fl) or estimated(F2).Results:A.albopictus exhibited increased fecundity and egg hatch success. Immature development was quick.Immature survival was high,with lowest rate in the pupal stage.Adult emergence was about 81%and sex ratio was close to 1:1.Generational mortality(K) was about 28%.A high proportion of females completed a reproductive cycle and the obtained parity rate was predicted to lead to higher fecundity in the next generation.Conclusios:It can be concluded that natural A.albopictus populations in Penang seem largely determined by quick development in combination with low immature loss and increased oviposition. | H Nur Aida Hamady Dieng Abu Hassan Ahmad Tomomitsu Satho AT Nurita MR Che Salmah Fumio Miake B Norasmah | 2011 | Asian Pacific Journal of Tropical Biomedicine2011，1，6： | 2 |
| 3 | Insecticide susceptibility of the dengue vector Aedes aegypti (Diptera: culicidae) in Makkah City, Saudi Arabia显示文摘 | Al Thbiani Aziz Hamady Dieng Ahmad Abu Hassan Tomomitsu Satho Fumio Miake Md Rawi Che Salmah Sazaly AbuBakar | 2011 | Asian Pacific Journal of Tropical Disease2011，，2： | 2 |
| 4 | Seasonal Changes in Mayfly Communities and Abundance in Relation to Water Physicochemistry in Two Rivers at Different Elevations in Northern Peninsular Malaysia显示文摘A field study was performed at rivers in Gunung Jerai forest reserve(Kedah,Malaysia) to assess seasonal changes in mayfly community structure and abundance in relation to altitude and water physicochemistry.Rivers at lower(Batu Hampar River) and higher(Teroi River) elevations were visited through dry and wet seasons in September 2007 to August 2008.Monthly visits were made to 20 sites on each river,and water and aquatic insects were sampled using D-pond aquatic nets.Water was warmer,more acid,and more turbid in Teroi River during wet season.Ammonia was the only nutrient exhibiting significant seasonal variations(greater during wet season).Chemical oxygen demand content was higher in Teroi River where biochemical oxygen demand content was low during wet season.Species richness was higher in Batu Hampar River,but displayed seasonal variations only in Teroi River.Among the eight families encountered,Baetidae was the commonest.Baetid abundance was usually high during wet season,and those belonging to the dominant genus(Baetis) were more abundant in Teroi River.Heptageniidae was the second commonest family;its predominant genus,Thalerospyrus was more abundant in Teroi River during dry season.Caenidae,Leptophlebiidae and Oligoneuriidae were only found in Batu Hampar River where their abundances peaked during dry season,i.e.,Habrophlebiodes sp.and Isonychia sp.Ephemerellidae and Teloganodidae occurred only in Teroi River,with the first found only during dry season.Mayflies were recorded under very distinct physicochemical conditions,illustrating their potential usefulness for assessing water quality.Caenids,leptophlebids,oligoneurids ephemerellids and teloganodids seem to be particularly sensitive to temperature,acidity,turbidity,chemical oxygen demand and biochemical oxygen demand,parameters that varied with river altitude. | Suhaila Abdul Hamid Mohd Ravi Che Salmah Hamady Dieng Abu Hassan Ahmad Tomomitsu Satho Fumio Miake | 2011 | 湿地科学2011，9，3： | 1 |
| 5 | Morphological and molecular characterization of fungus isolated from tropical bed bugs in Northern Peninsular Malaysia, Cimex hemipterus(Hemiptera: Cimicidae)显示文摘Objective:To investigate some morphological and molecular characteristics of fungal parasites isolated from wild tropical bed bug, Cimex hemipterus.Methods: A series of culture methods were used to obtain fungal isolates from i eld-collected bed bugs. Characteristics of the isolates such as colony appearance, mycelial texture and pigmentation were studied to explore their morphology. Isolates were also subjected to a PCRbased genotyping test.Results: There were noticeable dif erences in morphological characteristics among the four isolates. Conidial areas of one isolate were dark green, whereas those of the remaining colonies were olive-green, black or dark brown. Conidia of the dark green isolate were globose, while those of olive-green, black and dark brown isolates were globose to subglobose, globose to spherical and globose to subglobose/i nely roughened, respectively. These morphological specii cities and the molecular analyses showed that the fungal internal transcribed spacer ribosomal region and β-tubulin gene sequences of the isolates shared clade with Trichoderma and Aspergillus sequences.Conclusions: Overall, the new discovery of common pathogens in agricultural i eld developed in live bed bugs storage tank may initiate the use of biological agents in later years. | Abdul Hafiz Ab Majid Zulaikha Zahran Abd Hafis Abd Rahim Nor Azliza Ismail Wardah Abdul Rahman Kartiekasari Syahidda Mohammad Zubairi Hamady Dieng Tomomitsu Satho | 2015 | Asian Pacific Journal of Tropical Biomedicine2015，5，9： | 1 |
| 6 | Laboratory efficacy of mycoparasitic fungi(Aspergillus tubingensis and Trichoderma harzianum) against tropical bed bugs(Cimex hemipterus)(Hemiptera:Cimicidae)显示文摘Objective: To test the effectiveness of conidial spore formulations [Aspergillus tubingensis(A.tubingensis) and Trichoderma harzianum(T.harzianum)] against tropical bed bugs, Cimex hemipterus.Methods: Spore formulations were made from two fungal strains, T.harzianum and A.tubingensis.The bed bugs were exposed to the conidial spores placed soaked onto a fabric cloth for 1 h and the mortality counts were recorded daily until 14 days.Results: Mean survival times based on Kaplan–Meier survival analysis showed no significant differences between all the concentrations in both the fungal isolates:T.harzianum and A.tubingensis.However, the evaluation of both the isolates in terms of virulence resulted in low lethal hours in all the concentrations except for the high concentration of A.tubingensis(LT_(50)= 44.629 h) at the conidial exposure of 1 × 10~6 spores/mL.Rapid mortality of the bed bugs was observed from Day 6 to Day 12, ranging from 13% to 90% in all three concentrations of A.tubingensis.With reference to the T.harzianum exposure, the concentration of 1 × 10~4 spores/mL displayed a gradual increase in the percentage mortality of 90 on Day 14.Conclusions: Approaches to the bed bugs treatment should be explored in-depth using a natural biological agent like fungus especially A.tubingensis to reduce this pest population, in order to replace chemical methods. | Zulaikha Zahran Nik Mohd Izham Mohamed Nor Hamady Dieng Tomomitsu Satho Abdul Hafiz Ab Majid | 2017 | Asian Pacific Journal of Tropical Biomedicine2017，7，4： | 1 |
| 7 | Insecticide susceptibility of the dengue vector Aedes aegypti (Diptera: culicidae) in Makkah City, Saudi Arabia显示文摘 | Al Thbiani Aziz Hamady Dieng Ahmad Abu Hassan Tomomitsu Satho Fumio Miake Md Rawi Che Salmah Sazaly AbuBakar | 2011 | Asian Pacific Journal of Tropical Disease2011，，2： | 1 |
| 8 | Evaluation of the toxicity and repellency of tropical plant extract against subterranean termites,Globitermes sulphureus and Coptotermes gestroi显示文摘The harmful effects of chemical-based termiticides and the increased incidence of termite resistance have resulted in the need for safer and more effective termiticides.Therefore,the screening of antitermiticidal activity of naturally-occurring products could possibly hamper an alternative means in termite control strategies.The aims of this study were to determine the toxicity and repellency of L.leucocephala,A.paniculata,Az.indica and P.niruri crude extracts against two subterranean termites,G.sulphureus and C.gestroi.Bioassays were conducted by applying varying concentrations of the plant extracts(10,000 ppm,5000 ppm and 500 ppm)on both termite species under laboratory conditions.All extracts exhibited a significant antitermiticidal activity in time-and concentration-dependent manners after 14 days of exposure.The highest mortality of G.sulpureus and C.gestroi were noted in all methanolic extracts of P.niruri,L.leucocephala,A.paniculata,Az.indica at 10,000 ppm.High repellent activity was also noted in the choice bioassay when both termites were treated with all methanolic extracts at 10,000 ppm. | Noor Hazwani Bakaruddin Hamady Dieng Shaida Fariza Sulaiman Abdul Hafiz Ab Majid | 2018 | Information Processing in Agriculture2018，5，3： | 0 |
| 9 | Unusual developing sites of dengue vectors and potential epidemiological implications显示文摘Objective:To identify the unusual breeding sites of two dengue vectors,i.e.Aedes albopiclus(Ae.albopictus) and Aedes aegypti(Ae.aegypti).Methods:During the second half of 2010, we performed an occasional survey in rural(Teluk Tempoyak) and urban(Gelugor) areas of Penang Island,Malaysia,to identify cryptic breeding sites.Results:In the rural area,we found heterogeneous immature stages of Ae.albopiclus in the water bowl of an encaged bird.We also observed Ae.aegypti eggs deposited in the flush tank of a toilet in the urban area.Conclusions: It can be concluded that both breeding patterns can increase contact with hosts(humans and birds) and presumably population densities of Ae.albopictus and Ae.aegypti,thereby potentially boosting the risks for spread and transmission of arboviral diseases. | Hamady Dieng Rahman GM Saifur Abu Hassan Ahmad MR Che Salmah Al Thbiani Aziz Tomomitsu Satho Fumio Miake Zairi Jaal Sazaly Abubakar Ronald Enrique Morales | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，3： | 0 |
| 10 | Household survey of container-breeding mosquitoes and climatic factors influencing the prevalence of Aedes aegypti(Diptera:Culicidae)in Makkah City,Saudi Arabia显示文摘Objective:To investigate the prevalence of container breeding mosquitoes with emphasis on the seasonality and larval habitats of Aedes aegypti(Ae.aegypti) in Makkah City,adjoining an environmental monitoring and dengue incidence.Methods:Monthly visits were performed between April 2008 and March 2009 to randomly selected houses.During each visit,mosquito larvae were collected from indoors and outdoors containers by either dipping or pipetting. Mosquitoes were morphologically identified.Data on temperature,relative humidity,rain/ precipitations during the survey period was retrieved from governmental sources and analyzed. Results:The city was warmer in dry season(DS) than wet season(WS).No rain occurred at all during DS and even precipitations did fall,wetting events were much greaterduring WS.Larval survey revealed the co-breeding of Aedes,Culex and Anopheles in a variety of artificial containers in and around homes.32109 larvae representing 1st,2nd,3rd,and 4th stages were collected from 22618 container habitats.Culicines was far the commonest and Aedes genus was as numerous as the Culex population.Ae.aegypti larval abundance exhibited marked temporal variations,overall, being usually more abundant during WS.Ten types of artificial containers were found with developing larvae.70%of these habitats were located indoors.71.42%of indoor containers were permanent and 28.58* was semi-permanent during WS.Cement tanks was the only container type permanent during DS.Ae.aegypti larval indices(CI,HI,Bl) recorded were greater during WS. Conclusions:Taken together,these results indicate a high risk of dengue transmission in the holy city. | Al Thabiany Aziz Hamady Dieng Abu Hassan Ahmad Jazem A Mahyoub Abdulhafis M Turkistani Hatabbi Mesed Salah Koshike Tomomitsu Satho MR Che Salmah Hamdan Ahmad Wan Fatma Zuharah Ahmad Saad Ramli Fumio Miake | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，11： | 0 |
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