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| 1 | Novel functional proteins interact with midkine in hepatic cancer cells显示文摘BACKGROUND: Midkine is a heparin-binding growth factor that promotes the proliferation, survival, migration and differentiation of various target cells. Midkine plays an important role in tumorigenesis and tumor progression, and is overexpressed in many human malignant tumors. Patients with high tumor midkine expression frequently have a worse prognosis than those with low expression. The present study was designed to investigate the interaction network of midkine in hepatic cancer cells, and to elucidate its role in hepatocellular carcinoma. METHODS: DNA encoding full-length midkine was cloned into pDBLeu vector to serve as bait in yeast two-hybrid screening to identify interacting proteins. Candidate proteins were examined on SC-Leu-Trp-His+3-AT (20 mmol/L) plates and assayed for X-gal activity, then sequenced and classified according to the GenBank. Finally, identified proteins were expressed by the in vitro expression system pCMVTnT, and protein interactions were confirmed by co-immunoprecipitation. RESULTS: Using the yeast two-hybrid system, we found 6 proteins that interacted with midkine: NK-kappa-B inhibitor alpha (I-κ-B-α), Dvl-binding protein naked cuticle 2, granulin, latent active TGF-β binding protein 3, latent active TGF-β binding protein 4, and phospholipid scramblase 1. In vitro co-immunoprecipitation demonstrated that all identified proteins directly interacted with midkine.CONCLUSION: The identification of midkine-interacting proteins in hepatic cancer cells indicates that midkine is a multifunctional factor that may participate in cell migration, differentiation, and proliferation, and is also associated with the multicellular response feedback during the development of hepatocellular carcinoma. | Qiang Yan, Hui-Lian Huang, Xing Yao, Jing Li, Li-Qin Li, Jing Zhong, Li-Shan Min, Li-Cheng Dai and Shu-Sen Zheng Department of Hepatobiliary and Pancreatic Surgery, Huzhou Central Hospital (Yan Q and Yao X) Huzhou Key Laboratory of Molecular Medicine, Affiliated Central Hospital of Huzhou Teachers College (Huang HL, Li J, Li LQ, Zhong J, Min LS and Dai LC), Huzhou 313000, China Division of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China (Yan Q and Zheng SS) | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，3： | 7 |
| 2 | More synergetic cooperation of Yamanaka factors in induced pluripotent stem cells than in embryonic stem cells显示文摘在 pluripotency 的正式就职的核心管理者最近在体的房间 reprogramming 期间被发现了的 Yamanaka 因素的角色。我们的以前的学习发现 Yamanaka 因素在维持胚胎的茎(ES ) 房间 pluripotency 调整一个发展发信号网络。这里,我们完全证实 reprogrammed 导致了 pluripotent 茎(iPS ) 细胞并且由薄片薄片试金在这些细胞分析了 Yamanaka 因素的全球倡导者占有。我们发现 565 基因的倡导者是由在 iPS 房间的四个 Yamanaka 因素的合作界限, 10 褶层增加什么时候与他们在 ES 房间的绑定相比。,在 ES 房间 Oct4, Sox2,或 Klf4 在 iPS 房间在激活并且镇压的基因同等地散布的一个单个 Yamanaka 因素占据的倡导者在激活的主要在镇压基因和 c-Myc 散布了。薄片薄片数据的小径分析表明 Yamanaka 因素在 iPS 房间, 12 在之中是普通的调整了 16 条发展发信号小径, 4 与在 ES 房间调整的小径相比是唯一的。我们进一步在 iPS 房间分析了另一最近出版的薄片薄片数据集并且观察了类似的结果,显示出为揭示 pluripotency 维护和新生的性质的薄片薄片正小径分析的力量。下次,我们试验性地测试了压抑的发信号小径之一并且发现它的抑制确实改进了房间 reprogramming 的效率。一起拿,我们建议有一个核心为 pluripotency 必要的发展发信号网络与 TGF- ,刺猬, Wnt,是的 p53 压抑(殷) 管理者和 Jak-STAT,房间周期,焦点的粘附, adherens 连接作为活跃(杨) ;并且 Yamanaka 因素 synergistically 在 Yin-Yang 调整他们导致 pluripotency 的平衡方法。 | Jinyan Huang Taotao Chen Xiaosong Liu Jing Jian Jinsong Li Dangsheng Li X Shirley Liu Wei Li Jiuhong Kang Gang Pei | 2009 | Cell Research2009，19，10： | 4 |
| 3 | Simultaneous multi-tear exclusion:an optimal strategy for type B thoracic aortic dissection initially proved by a single center's 8 years experience显示文摘背景 Endovascular 接枝stent 广泛地被用来对待胸的大动脉的 dissection.However ,很少信息都不关于跟随八年用为斯坦福的治疗的同时的多眼泪排除的经验打 B 胸的大动脉的解剖导致到1月2006,29日类型 B 胸的大动脉的解剖病人的从1998年9月的没有不利 events.Methods 的成功的大动脉的改变的多重 tears.This 报告细节的同时的排除的结果是可得到的( 24 个人, 5 | YUAN Liang-xi BAO Jun-min ZHAO Zhi-qing FENG Xiang OU Le-feng FENG Rui LU Qing-sheng MEI Zhi-jun JING Zai-ping Department of Vascular Surgery,Changhai Hospital,Second Military Medical University Institute of Vascular Surgery of Chinese People’s Liberation Army Clinic Center of Vascular Diseases of Shanghai National Key Department of Vascular Surgery of China Shanghai 200433,China (Yuan LX,Bao JM,Zhao ZQ,Feng X,Ou LF,Feng R,Lu QS,Mei ZJ and Jing ZP) | 2007 | Chinese Medical Journal2007，，24： | 3 |
| 4 | Proliferation and pluripotency potential of ectomesenchymal cells derived from first branchial arch显示文摘 | Lin Y Yan Z Liu L Qiao J Jing W Wu L Chert X Li Z Tang W Zheng X Tian W | 2006 | 中国生物学文摘2006，20，5： | 3 |
| 5 | Raman Spectroscopic Study on the Microstructure of GeS2-Ga2S3-KCl Glasses显示文摘 | H Z Tao X J Zhao C B Jing | | 0，，： | 2 |
| 6 | Docosahexaenoic acid suppresses arachidonic acid-induced proliferation of LS-174T human colon carcinoma cells显示文摘AIM:To investigate the impact of arachidonic acid (AA) and docosahexaenoic acid (DHA) and their combination on colon cancer cell growth.METHODS:The LS-174T colon cancer cell line was used to study the role of the prostaglandin precursor AA and the omega-3 polyunsaturated fatty acid DHA on cell growth. Cell viability was assessed in XTT assays. For analysis of cell cycle and cell death,flow cytometry and DAPI staining were applied. Expression of cyclooxygenase-2 (COX-2),p21 and bcl-2 in cells incubated with AA or DHA was examined by real-time RT-PCR. Prostaglandin E2 (PGE2) generation in the presence of AA and DHA was measured using a PGE2-ELISA.RESULTS:AA increased cell growth,whereas DHAreduced viability of LS 174T cells in a time-and dose-dependent manner. Furthermore,DHA down-regulated mRNA of bcl-2 and up-regulated p21. Interestingly,DHA was able to suppress AA-induced cell proliferation and significantly lowered AA-derived PGE2 formation. DHA also down-regulated COX-2 expression. In addition to the effect on PGE2 formation,DHA directly reduced PGE2-induced cell proliferation in a dose-dependent manner. CONCLUSION:These results suggest that DHA can inhibit the pro-proliferative effect of abundant AA or PGE2. | Piet Habbel Karsten H Weylandt Katja Lichopoj Johannes Nowak Martin Purschke Jing-Dong Wang Cheng-Wei He Daniel C Baumgart Jing X Kang | 2009 | World Journal of Gastroenterology2009，15，9： | 2 |
| 7 | Overview to the Hard X-ray Modulation Telescope (Insight-HXMT) Satellite显示文摘As China’s first X-ray astronomical satellite, the Hard X-ray Modulation Telescope (HXMT), which was dubbed as Insight-HXMT after the launch on June 15, 2017, is a wide-band(1-250 ke V) slat-collimator-based X-ray astronomy satellite with the capability of all-sky monitoring in 0.2-3 Me V. It was designed to perform pointing, scanning and gamma-ray burst(GRB)observations and, based on the Direct Demodulation Method (DDM), the image of the scanned sky region can be reconstructed.Here we give an overview of the mission and its progresses, including payload, core sciences, ground calibration/facility, ground segment, data archive, software, in-orbit performance, calibration, background model, observations and some preliminary results. | Shuang-Nan Zhang TiPei Li FangJun Lu LiMing Song YuPeng X u CongZhan Liu Yong Chen XueLei Cao QingCui Bu Zhi Chang Gang Chen Li Chen TianXiang Chen YiBao Chen YuPeng Chen Wei Cui WeiWei Cui JingKang Deng YongWei Dong Yuan Yuan Du MinXue Fu GuanHua Gao He Gao Min Gao MingYu Ge YuDong Gu Ju Guan Can Gungor ChengCheng Guo DaWei Han Wei Hu Yue Huang Jia Huo ShuMei Jia LuHua Jiang WeiChun Jiang Jing Jin YongJie Jin Bing Li ChengKui Li Gang Li MaoShun Li Wei Li Xian Li XiaoBo Li XuFang Li YanGuo Li ZiJian Li ZhengWei Li XiaoHua Liang JinYuan Liao GuoQing Liu HongWei Liu ShaoZhen Liu XiaoJing Liu Yuan Liu YiNong Liu Bo Lu XueFeng Lu Tao Luo Xiang Ma Bin Meng Yi Nang JianYin Nie Ge Ou JinLu Qu Na Sai RenCheng Shang GuoHong Shen Liang Sun Ying Tan Lian Tao YouLi Tuo Chen Wang ChunQin Wang GuoFeng Wang HuanYu Wang Juan Wang WenShuai Wang YuSa Wang XiangYang Wen BaiYang Wu BoBing Wu Mei Wu GuangCheng Xiao ShaoLin Xiong LinLi Yan JiaWei Yang Sheng Yang YanJi Yang QiBin Yi Bin Yuan AiMei Zhang ChunLei Zhang ChengMo Zhang Fan Zhang HongMei Zhang Juan Zhang Qiang Zhang ShenYi Zhangs Shu Zhang Tong Zhang WanChang Zhang Wei Zhang WenZhao Zhang Yi Zhang YiFei Zhang YongJie Zhang Yue Zhang Zhao Zhang Zhi Zhang ZiLiang Zhang HaiSheng Zhao XiaoFan Zhao ShiJie Zheng JianFeng Zhou YuXuan Zhu Yue Zhu RenLin Zhuang | 2020 | Science China(Physics,Mechanics & Astronomy)2020，63，4： | 2 |
| 8 | Microstructural Probing of (1-x)GeS2-xGa2S3 System Glasses By Raman Scattering显示文摘 | H Z Tao X J Zhao C B Jing S Liu | | Journal of Wuhan University of Technology (Materials Science Edition)0，，： | 2 |
| 9 | Raman Scattering Studies of the GeS2-Ga2 S3-CsCl Glassy System显示文摘 | H Z Tao X J Zhao C B Jing H Yang,S Mao | | 0，，： | 2 |
| 10 | Microstructure and Thermal Properties Analysis of (1-x) As2S3-xCdBr2 Glass System显示文摘 | H Z Tao X J Zhao C B Jing X H Wang | | Journal of Wuhan University of Technology (Materials Science Edition)0，，： | 2 |
| 11 | 呼出气冷凝液代谢组学对慢加急性肝衰竭预后的预测价值显示文摘[据Hepatology Research 2021年4月报道]题:呼出气冷凝液代谢组学对慢加急性肝衰竭预后的预测价值(作者Jing J等)慢加急性肝衰竭(ACLF)是导致慢性失代偿肝病患者死亡的重要原因之一,也是国内外临床肝病研究领域的热点和难题。部分ACLF患者在治疗“黄金窗”阶段通过积极救治,能够获得可逆性的恢复,因此早期预测ACLF预后对患者及早施救以及重症化防控十分有益。 | 景婧 牛明 王睿林 JING J SANG X YOU S | 2021 | 临床肝胆病杂志2021，37，7： | 2 |
| 12 | In vivo chondrogenesis of adult bone-marrow-derived autologous mesenchymal stem cells显示文摘 | Chen J Wang C Lu S Wu J Guo X Duan C Dong L Song Y Zhang J Jing D Wu L Ding J Li D | 2006 | 中国生物学文摘2006，20，5： | 2 |
| 13 | A new SPD process for spheroidal cast iron显示文摘 | Zhao X Jing T F Gao Y W | 2004 | Materials Letters2004，58，： | 1 |
| 14 | Review of surface photovoltage spectra ofnano sized semiconductor and its applications in heterogeneous photoeatalysis 显示文摘 | Jing L Q Sun X J Shang J | 2003 | Solar Energy Materials And Solar Cells2003，79，2： | 1 |
| 15 | A formaldehyde-free flame retardant wood particleboard system based on two component polyurethane adhesive 显示文摘 | Tang Y Wang D Y Jing X K | 2008 | J App Polym Sci2008，108，： | 1 |
| 16 | Large-scale variability of wind erosion mass flux rates at Owens Lake显示文摘 | Gillette D A Fryrear D W Jing B X | 1997 | Journal of Geophysical Research1997，102，25： | 1 |
| 17 | Plant proteomics in the post-genomic era显示文摘 | Guo Y M Shen S H Jing Y X | 2002 | Acta Bot Sin2002，44，6： | 1 |
| 18 | Investigation on 1,4-Dichlorophthalazine-derivetized Cinchona Alkaloids-catalyzed Asymmetric 'Interrupted' Feist-B6nary Reaction 显示文摘 | Jin Y Liu X Y Jing L L | 2007 | Chirality2007，19，5： | 1 |
| 19 | Geographical variationinegg cold hardiness: Astudyontheadaptation strategiesof themigratorylocust Locusta migratoriaL显示文摘 | Jing X H Kang L | 2003 | Ecological Entomology2003，28，： | 1 |
| 20 | A probabilistic model applied to emergency service vehicle location显示文摘 | Hari K Rajagopalan Cem Saydamb Jing X | 2009 | European Journal of Operational Research2009，196，1： | 1 |
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