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| 1 | Methods for the extraction and RNA profiling of exosomes显示文摘AIM:To develop protocols for isolation of exosomes and characterization of their RNA content.METHODS:Exosomes were extracted from He La cell culture media and human blood serum using the Total exosome isolation(from cell culture media)reagent,and Total exosome isolation(from serum)reagent respectively.Identity and purity of the exosomes was confirmed by Nanosight?analysis,electron microscopy,and Western blots for CD63 marker.Exosomal RNA cargo was recovered with the Total exosome RNA and protein isolation kit.Finally,RNA was profiled using Bioanalyzer and quantitative reverse transcriptionpolymerase chain reaction(q RT-PCR)methodology.RESULTS:Here we describe a novel approach for robust and scalable isolation of exosomes from cell culture media and serum,with subsequent isolation and analysis of RNA residing within these vesicles.The isolation procedure is completed in a fraction of the time,compared to the current standard protocols utilizing ultracentrifugation,and allows to recover fully intact exosomes in higher yields.Exosomes were found tocontain a very diverse RNA cargo,primarily short sequences 20-200 nt(such as mi RNA and fragments of m RNA),however longer RNA species were detected as well,including full-length 18S and 28S r RNA.CONCLUSION:We have successfully developed a set of reagents and a workflow allowing fast and efficient extraction of exosomes,followed by isolation of RNA and its analysis by q RT-PCR and other techniques. | Emily Zeringer Mu Li Tim Barta Jeoffrey Schageman Ketil Winther Pedersen Axl Neurauter Susan Magdaleno Robert Setterquist Alexander V Vlassov | 2013 | World Journal of Methodology2013，3，1： | 5 |
| 2 | External Validation of the Fatty Liver Index for Identifying Nonalcoholic Fatty Liver Disease in a Population-based Study显示文摘 | Edith M. Koehler Jeoffrey N.L. Schouten Bettina E. Hansen Albert Hofman Bruno H. Stricker Harry L.A. Janssen | 2013 | Clinical Gastroenterology and Hepatology2013，，： | 4 |
| 3 | Prevalence and risk factors of non-alcoholic fatty liver disease in the elderly: Results from the Rotterdam study显示文摘 | Edith M. Koehler Jeoffrey N.L. Schouten Bettina E. Hansen Frank J.A. van Rooij Albert Hofman Bruno H. Stricker Harry L.A. Janssen | 2012 | Journal of Hepatology2012，，6： | 2 |
| 4 | Benign blue nevus of the uterine cervix 显示文摘 | Majmudar B Jeoffrey Ross R Gorelkin L | 1979 | Am J Obster Gvnecol1979，134，： | 2 |
| 5 | Recreational value, user heterogeneity and site characteristics in contingent valuation 显示文摘 | Benedicte Rulleau Jeoffrey Dehez Patrick Point | 2012 | Tourism Management2012，，1： | 1 |
| 6 | Treatment and outcomes of poly- merase chain reaction-confirmed early syphilis 显示文摘 | Read PJ Guy R Jeoffreys N | 2015 | Sex Health2015，6，3： | 1 |
| 7 | A longitudinal evaluation of Treponema pallidum PCR testing in early syphilis显示文摘 | SHIELDS M GUY R J JEOFFREYS N J | 2012 | BMC Infect Dis2012，12，： | 1 |
| 8 | A longitudinal evaluation of Treponema pallidum PCR testing in early syphilis 显示文摘 | Shields M Guy R J Jeoffreys N J et at | 2012 | BMC Infect Dis2012，12，： | 1 |
| 9 | Analysis of the RNA content of the exosomes derived from blood serum and urine and its potential as biomarkers显示文摘 | Mu Li Emily Zeringer Timothy Barta Jeoffrey Schageman Angie Cheng Alexander V. Vlassov | 2014 | Philosophical Transactions of the Royal Society B: Biological Sciences . 2014 (1652)2014，，： | 1 |
| 10 | Statin therapy is associated with a reduced risk of non-alcoholic fatty liver in overweight individuals显示文摘 | Catherine E. de Keyser Edith M. Koehler Jeoffrey N.L. Schouten Loes E. Visser Albert Hofman Harry L.A. Janssen Bruno H. Stricker | 2014 | Digestive and Liver Disease2014，，： | 1 |
| 11 | Recreational value, user heterogeneity and site characteristics in contingent valuation显示文摘 | Bndicte Rulleau Jeoffrey Dehez Patrick Point | 2012 | Tourism Management2012，33，1： | 1 |
| 12 | Clinical utility of urinary cytology to detect BK viral nephropathy显示文摘 | Nankivell B J Renthawa J Jeoffreys N | 2015 | Transplantation2015，3，12： | 1 |
| 13 | Clinical Utility of Urinary Cytology to Detect BK Viral Nephropathy显示文摘 | Nankivell B J Renthawa J Jeoffreys N | 2015 | Transplantation2015，99，8： | 1 |
| 14 | Psoriasis is independently associated with nonalcoholic fatty liver disease in patients 55 years old or older: Results from a population-based study显示文摘 | Ella A.M. van der Voort Edith M. Koehler Emmilia A. Dowlatshahi Albert Hofman Bruno H. Stricker Harry L.A. Janssen Jeoffrey N.L. Schouten Tamar Nijsten | 2013 | Journal of the American Academy of Dermatology2013，，： | 1 |
| 15 | A longitudinal evaluation of Treponema pallidum PCR testing in early syphilis显示文摘 | Shields M Guy RJ Jeoffreys NJ | 2012 | BMC Infect Dis2012，，12： | 1 |
| 16 | Azithromycin-resist- ant syphilis-causing strains in Sydney, Australia: preva- lence and risk factors 显示文摘 | Read P Jeoffreys N Tagg K | 2014 | J Clin Microbiol2014，52，8： | 1 |
| 17 | Clinical utility of urinary cytology to detect BK viral nephropathy显示文摘 | Nankivell BJ Renthawa J Jeoffreys N | 2015 | Transplantation2015，99，8： | 1 |
| 18 | Analysis of Irrigation Systems Employing Comparative Performance Indicators: A Benchmark Study for National Irrigation and Communal Irrigation Systems in Cagayan River Basin显示文摘 | Jeoffrey Lloyd Reyes Bareng Orlando Florendo Balderama Lanie Alejandro Alejo | 2015 | Journal of Agricultural Science and Technology(A)2015，5，5： | 0 |
| 19 | Surveillance of Australian Hajj pilgrims for carriage of potentially pathogenic bacteria:Data from two pilot studies显示文摘AIM To estimate the pharyngeal carriage rate of Neisseria meningitidis(N. meningitidis), Streptococcus pneumoniae(S. pneumoniae) and Staphylococcus aureus(S. aureus) among Australian Hajj pilgrims.METHODS In 2014, surveillance was conducted in two phases among Australian Hajj pilgrims: The first phase during Hajj in Mina, and the second phase soon after returning home to Australia. Nasopharyngeal or oropharyngeal swabs were taken from participants then tested, firstly by nucleic acid testing, and also by standard culture.RESULTS Of 183 participants recruited in the first phase, 26(14.2%) tested positive for S. pneumoniae; 4 had received pneumococcal conjugate vaccine(PCV13). Only one tested positive for N. meningitidis(W). Of 93 2^(nd) phase samples cultured, 17(18.3%) grew S. aureus, all methicillin sensitive, 2(2.2%) grew N. meningitidis(on subculture; one serotype B, one negative), and 1(1%), from an unvaccinated pilgrim, grew S. pneumoniae.CONCLUSION Relatively high carriage of S. pneumoniae and little meningococcal carriage was found. This indicates the importance of a larger study for improved infection surveillance and possible vaccine evaluation. | Mohammad Irfan Azeem Mohamed Tashani Al-Mamoon Badahdah Leon Heron Kristen Pedersen Neisha Jeoffreys Jen Kok Elizabeth Haworth Dominic E Dwyer Grant Hill-Cawthorne Harunor Rashid Robert Booy | 2017 | World Journal of Clinical Cases2017，5，3： | 0 |
| 20 | 亚太航空公司以非合并方式迎战危机显示文摘在欧洲消沉的日子里,法航前总裁克里斯蒂安·布兰克1996年大声疾呼:“我们在为法航集团而战,更是为法国而战。”表达了这个国家航空公司与法国精神难解难分的关系。 | 张景银 Jeoffrey Thomas | 2009 | 空运商务2009，，10： | 0 |
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