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| 1 | Characterization of a Novel β-thioglucosidase CpTGG1 in Carica papaya and its Substrate-dependent and Ascorbic Acid-independent O-β-glucosidase Activity显示文摘Plant thioglucosidases are the only known S-glycosidases in the large superfamily of glycosidases.These enzymes evolved more recently and are distributed mainly in Brassicales.Thioglucosidase research has focused mainly on the cruciferous crops due to their economic importance and cancer preventive benefits.In this study,we cloned a novel myrosinase gene,CpTGG1,from Carica papaya Linnaeus.and showed that it was expressed in the aboveground tissues in planta.The recombinant CpTGG1 expressed in Pichia pastoris catalyzed the hydrolysis of both sinigrin and glucotropaeolin(the only thioglucoside present in papaya),showing that CpTGG1 was indeed a functional myrosinase gene.Sequence alignment analysis indicated that CpTGG1 contained all the motifs conserved in functional myrosinases from crucifers,except for two aglycon-binding motifs,suggesting substrate priority variation of the non-cruciferous myrosinases.Using sinigrin as substrate,the apparent K m and V max values of recombinant CpTGG1 were 2.82 mM and 59.9 μmol min-1 mg protein-1,respectively.The Kcat K m value was 23 s -1 mM -1.O-β-glucosidase activity towards a variety of substrates were tested,CpTGG1 displayed substrate-dependent and ascorbic acid-independent O-β-glucosidase activity towards 2-nitrophenyl-βD-glucopyranoside and 4-nitrophenyl-β-D-glucopyranoside,but was inactive towards glucovanillin and n-octyl-β-D-glucopyranoside.Phylogenetic analysis indicated CpTGG1 belongs to the MYR II subfamily of myrosinases. | Han Nong Jia-Ming Zhang Ding-Qin Li Meng Wang Xue-Piao Sun Yun Judy Zhu Johan Meijer Qin-Huang Wang | 2010 | Journal of Integrative Plant Biology2010，52，10： | 5 |
| 2 | Excimer laser micromachining for 3D microstructure显示文摘 | Kyung Hyun Choi Johan Meijer Takahisa Masuzawa | 2004 | Journal of Materials Processing Technology2004，149，： | 1 |
| 3 | Myrosinases from root and leaves of Arabidopsis thaliana have different catalytic properties显示文摘 | Derek Andersson Romit Chakrabarty Sarosh Bejai Jiaming Zhang Lars Rask Johan Meijer | 2009 | Phytochemistry2009，，11： | 1 |
| 4 | Transcriptional profiling by cDNA-AFLP reveals novel insights during methyl jasmonate, woun- ding and insectattack in Brassica napus 显示文摘 | Bejai R Sarosh Johan Meijer | 2007 | Plant Mol Bio2007，64，512： | 1 |
| 5 | Myrosinase: gene family evolution and herbivore defense in Brassicaceae显示文摘 | Lars Rask Erik Andréasson Barbara Ekbom Susanna Eriksson Bo Pontoppidan Johan Meijer | 2000 | Plant Molecular Biology2000，，1： | 1 |
| 6 | 显示文摘 | Johan J F G A de Brabander-van den Berg E M M Meijer E W | 1994 | Science1994，266，5188： | 1 |
| 7 | Encapsulation of guest molecules into a dendritic box 显示文摘 | Johan F G A Jansen Ellen M M de Brabander-van den Berg Meijer E W | 1994 | Science1994，266，18： | 1 |
| 8 | Differences in metabolism between adeno- and squamous cell non-small cell lung carcinomas: Spatial distribution and prognostic value of GLUT1 and MCT4显示文摘 | Tineke W.H. Meijer Olga C.J. Schuurbiers Johannes H.A.M. Kaanders Monika G. Looijen-Salamon Lioe-Fee de Geus-Oei Ad F.T.M. Verhagen Jasper Lok Henricus F.M. van der Heijden Saskia E. Rademakers Paul N. Span Johan Bussink | 2011 | Lung Cancer2011，，3： | 1 |
| 9 | Transcriptional profiling by cDNA-AFLP reveals novel insights during methyl jasmonate, wounding and insect attack in Brassica napus显示文摘 | Bejai R. Sarosh Johan Meijer | 2007 | Plant Molecular Biology2007，，4： | 1 |
| 10 | Induction of cytosolic and microsomal epoxide hydrolases and proliferation of peroxisomes and mitochondria in mouse liver after dietary exposure to p-chlorophenoxyacetic acid, 2, 4-dichlorophenoxyacetic acid and 2, 4, 5-trichlorophenoxyacetic acid 显示文摘 | Bo Lundgren Johan Meijer Joseph W Depierre | 1987 | Biochemical Pharmacology1987，36，6： | 1 |
| 11 | Laser Beam Machining (LBM), State of the Art and New Opportunities显示文摘 | Johan Meijer | 2004 | Journal of Materials Processing Technology2004，149，13： | 1 |
| 12 | Encapsulation of guest molecules into a dendritic box显示文摘 | Johan F G A Jansen E W Meijer | 1994 | Science1994，266，： | 1 |
| 13 | Taxonomic characterization and plant colonizing abilities of some bacteria related to Bacillus amyloliquefaciens and Bacillus subtilis显示文摘 | Oleg N. Reva Christina Dixelius Johan Meijer Fergus G. Priest | 2004 | FEMS Microbiology Ecology2004，，2： | 1 |
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