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| 1 | Transcriptomics-based identification of WRKY genes and characterization of a salt and hormone-responsive PgWRKY1 gene in Panax ginseng显示文摘WRKY 蛋白质属于一个抄写因素(TF ) 家庭和戏在许多植物过程,对不能生活、关於生命的压力的包括的植物回答,以及第二等的新陈代谢的动态角色。然而,在人参属人参 C ·迈耶的 WRKY 基因都没迄今为止被报导。在这研究,从甲基 jasmonate (MeJA ) 的很多 WRKY unigenes 对待偶然这种的根 transcriptome 用下一代的定序被识别技术。编码 WRKY 蛋白质的 48 有希望的 WRKY unigenes 的一个总数被消除冤屈和不完全的开的读物框架(ORF ) 获得。种系发生的分析揭示 48 WRKY TF,包括 11 组我, 36 组 II,和 1 组 III。而且,指定为 PgWRKY1 的一 MeJA 应答的 unigene 被克隆并且描绘。它包含 1077 bp 的全部 ORF 并且编码 358 氨基酸残余的多肽。PgWRKY1 蛋白质包含由保存氨基酸顺序主题 WRKYGQK 和 C 2 H 2-type 锌手指主题属于 WRKY 亚群 II-d。在洋葱和烟草外皮房间的 PgWRKY1-GFP 熔化蛋白质的 Subcellular 本地化表明 PgWRKY1 在原子核是只在场的。量的即时聚合酶链反应分析证明 PgWRKY1 的表示与叶子,茎,和种子相比在根和侧面的根是相对更高的。重要地, PgWRKY1 表示被水杨酸显著地导致酸, abscisic 酸,和 NaCl,而是由 MeJA 处理的 downregulated。这些结果建议 PgWRKY1 可能是对荷尔蒙和盐压力作出回应的多重压力可诱导的基因。 | Mohammed Nuruzzaman Hongzhe Cao Hao Xiu Tiao Luo Jijia Li Xianghui Chen Junli Luo Zhiyong Luo | 2016 | Acta Biochimica et Biophysica Sinica2016，48，2： | 12 |
| 2 | Regulation of polyphenols accumulation by combined overexpression/silencing key enzymes of phyenylpropanoid pathway显示文摘对有象 chlorogenic 酸(CGA ) 和卢丁那样的增加的理想的多酚的植物的新陈代谢的工程有成长兴趣。在这研究,两本氨基丙酸氨 lyase (AtPAL2 ) 的 overexpression 的效果, phenylpropanoid 小径的第一酶,和 hydroxycinnamoyl-CoA, quinate:hydroxycinnamoyl transferase (NtHQT ) , CGA 生合成的最后酶,和和 NtHQT 的 silencing 的 AtPAL2 的 overexpression 在烟草被调查。转基因的烟草植物 overexpressing AtPAL2 证明二和五预定 CGA 和卢丁层次的增加比野类型(WT ) 分别地种。NtHQT 的 Overexpression 进一步比 WT 水平的在 AtPAL2 植物增加 CGA 的累积到大约三次,当在 AtPAL2 的 NtHQT 的 silencing 比 WT 植物的在卢丁水平的 12 次增加种结果时。本氨基丙酸氨 lyase (PAL ) 和 overexpression/silencing HQT 的同时的 overexpression 能与增加的多酚被用于功能的食物的生产。 | Junli Chang Jie Luo Guangyuan He | 2009 | Acta Biochimica et Biophysica Sinica2009，41，2： | 12 |
| 3 | Screen-imaging guidance using a modified portable video macroscope for middle cerebral artery occlusion显示文摘The use of operating microscopes is limited by the focal length.Surgeons using these instruments cannot simultaneously view and access the surgical field and must choose one or the other.The longer focal length (more than 1 000 mm) of an operating telescope permits a position away from the operating field,above the surgeon and out of the field of view.This gives the telescope an advantage over an operating microscope.We developed a telescopic system using screen-imaging guidance and a modified portable video macroscope constructed from a Computar MLH-10 × macro lens,a DFK-21AU04 USB CCD Camera and a Dell laptop computer as monitor screen.This system was used to establish a middle cerebral artery occlusion model in rats.Results showed that magnification of the modified portable video macroscope was appropriate (5-20 ×) even though the Computar MLH-10 × macro lens was placed 800 mm away from the operating field rather than at the specified working distance of 152.4 mm with a zoom of 1-40 ×.The screen-imaging telescopic technique was clear,life-like,stereoscopic and matched the actual operation.Screen-imaging guidance led to an accurate,smooth,minimally invasive and comparatively easy surgical procedure.Success rate of the model establishment evaluated by neurological function using the modified neurological score system was 74.07%.There was no significant difference in model establishment time,sensorimotor deficit and infarct volume percentage.Our findings indicate that the telescopic lens is effective in the screen surgical operation mode referred to as 'long distance observation and short distance operation' and that screen-imaging guidance using an modified portable video macroscope can be utilized for the establishment of a middle cerebral artery occlusion model and micro-neurosurgery. | Xingbao Zhu Junli Luo Yun Liu Guolong Chen Song Liu Qiangjin Ruan Xunding Deng Dianchun Wang Quanshui Fan Xinghua Pan | 2012 | Neural Regeneration Research2012，7，12： | 6 |
| 4 | Hexokinase II promotes the Warburg effect by phosphorylating alpha subunit of pyruvate dehydrogenase显示文摘Objective: Tumor cells rely heavily on glycolysis regardless of oxygen tension, a phenomenon called the Warburg effect. Hexokinase II(HKII) catalyzes the first irreversible step of glycolysis and is often overexpressed in tumor cells. Mitochondrial HKII couples glycolysis and oxidative phosphorylation while maintaining mitochondrial membrane integrity. In this study, we investigated the role of HKII in promoting the Warburg effect in cancer cells.Methods: HKII-mediated phosphorylation of the alpha subunit of pyruvate dehydrogenase(PDHA1) was tested in HEK293 T cells and clear cell renal cell carcinoma(cc RCC) specimens using gene knockdown, western blotting,immunohistochemistry, and immunofluorescence.Results: It was determined that HKII could not only transform glucose into glucose-6-phosphate, but also transfer the phosphate group of ATP onto PDHA1. In addition, it was found that HKII increased the phosphorylation of Ser293 on PDHA1, decreasing pyruvate dehydrogenase(PDH) complex activity and thus rerouting the metabolic pathway and promoting the Warburg effect. The overexpression of HKII correlated with the phosphorylation of PDHA1 and disease progression in cc RCC.Conclusions: The data presented here suggest that HKII is an important biomarker in the evaluation and treatment of cancer. | Fangxiu Luo You Li Fei Yuan Junli Zuo | 2019 | Chinese Journal of Cancer Research2019，31，3： | 4 |
| 5 | Uptake of oxidative stress-mediated extracellular vesicles by vascular endothelial cells under low magnitude shear stress显示文摘Extracellular vesicles(EVs)are increasingly used as delivery vehicles for drugs and bioactive molecules,which usually require intravascular administration.The endothelial cells covering the inner surface of blood vessels are susceptible to the shear stress of blood flow.Few studies demonstrate the interplay of red blood cell-derived EVs(RBCEVs)and endothelial cells.Thus,the phagocytosis of EVs by vascular endothelial cells during blood flow needs to be elucidated.In this study,red blood cell-derived extracellular vesicles(RBCEVs)were constructed to investigate endothelial cell phagocytosis in vitro and animal models.Results showed that low magnitude shear stress including low shear stress(LSS)and oscillatory shear stress(OSS)could promote the uptake of RBCEVs by endothelial cells in vitro.In addition,in zebrafish and mouse models,RBCEVs tend to be internalized by endothelial cells under LSS or OSS.Moreover,RBCEVs are easily engulfed by endothelial cells in atherosclerotic plaques exposed to LSS or OSS.In terms of mechanism,oxidative stress induced by LSS is part of the reason for the increased uptake of endothelial cells.Overall,this study shows that vascular endothelial cells can easily engulf EVs in areas of low magnitude shear stress,which will provide a theoretical basis for the development and utilization of EVs-based nano-drug delivery systems in vivo. | Xian Qin Kun Zhang Juhui Qiu Nan Wang Kai Qu Yuliang Cui Junli Huang Li Luo Yuan Zhong Tian Tian Wei Wu Yi Wang Guixue Wang | 2022 | Bioactive Materials2022，7，3： | 3 |
| 6 | Time Sequence of Himalayan Endogenetic Mineralization on the East Side of the Qinghai-Tibet Plateau显示文摘Among the endogenetic deposits in the Sanjiang area and at the west margin of the Yangtze platform, Himalayan deposits are the most important and contribute a large proportion of the resources of superlarge deposits. Among the controlled resources of this region, 84% of copper resources, 67% of Pb-Zn, 31% of Ag, 77% of gold and 24% of tin come from Himalayan deposits on the east side of the Qinghai-Tibet plateau. Himalayan endogenetic mineralization shows a relatively complete sequence evolution in the Sanjiang area and on the west margin of the Yangtze platform. Mineralization is manifested by gold deposits related to K-rich lamprophyre, REE deposits related to alkalic complexes and Cu-Au-polymetallic deposits related to alkaline porphyry. Six sequences of mineralization evolution since 65 Ma B.P. in the Sanjiang area and on the west side of the Yangtze platform can be recognized. Himalayan endogenetic mineralization on the east side of the Qinghai-Tibet plateau reached its peak before the Oligo-cene, | LUO Junlie CHEN Yuchuan WANG Denghong | 2000 | Acta Geologica Sinica(English Edition)2000，74，3： | 2 |
| 7 | Main Mechanism for Generating Overpressure in the Paleogene Source Rock Series of the Chezhen Depression,Bohai Bay Basin, China显示文摘The Chezhen depression,located in the south of Bohai Bay Basin,is an oil-producing basin in China. The third and fourth members of the Shahejie Formation(E(s3) and E(s4)) are the main source rock series in the Chezhen depression. Widespread overpressures occurred in the E(s3) and E(s4) from the depths of approximately 2 000 to 4 600 m,with the maximum pressure coefficient of 1.98 from drillstem tests(DST). Among the sonic,resistivity and density logs,sonic-log is the only reliable pressure indicator and can be used to predict the pore pressure with Eaton's method. All the overpressured mudstones in the source rock series have higher acoustic traveltimes compared with normally pressured mudstones at a given depth. The overpressured mudstones in the E(s3) and E(s4) units are characterized by a normal geothermal gradient,high average density values up to 2.5 g/cm^3,strong present-day hydrocarbon generation capability,abundant mature organic matter and high contents of residual hydrocarbons estimated by the Rock-Eval S1 values and chloroform-soluble bitumen 'A' values. All suggest that the dominant mechanism for overpressure in the mudstones of source rock series in the Chezhen depression is hydrocarbon generation. A comparison between the matrix porosity of the normally pressured sandstones and overpressured sandstones,the quantitative evaluation of porosity loss caused by compaction and the conventional thin section inspection demonstrate that the sandstones in the Chezhen depression were normally compacted. The high contents of hydrocarbons in the overpressured reservoirs prove that the overpressure in the sandstones of the source rock series was caused by pressure transmission from the source rocks. | Junli Zhang Sheng He Yuqin Wang Yongshi Wang Xuefeng Hao Shengyuan Luo Ping Li Xuewei Dang Ruizhi Yang | 2019 | Journal of Earth Science2019，30，4： | 2 |
| 8 | Occlusion of the middle cerebral artery Guidance by screen imaging using an EDA-H portable medium-soft electronic endoscope显示文摘The present study aimed to verify the practicability of performing screen-based surgical operations under the guidance of a real-time viewing and recording system (RTVRS) using a portable medium-soft electronic endoscope (pmsEE).The middle cerebral artery in rats was occluded under screen-imaging guidance using a pmsEE RTVRS to reproduce an animal model of human cerebral infarction.The screen imaging of the pmsEE RTVRS was clear,life-like,stereoscopic and synchronous with the actual operation.Screen-imaging guidance led to an accurate,smooth,minimally invasive and comparatively easy surgical procedure.The surgical success rate,time of model establishment,neurological function scores,and infarct volume were similar to those using an operating microscope.These results indicate that the self-designed pmsEE RTVRS could be utilized for portable endoscopic screen-based surgical operations. | Xingbao Zhu Junli Luo Song Liu Dongping Li Min Li Quanshui Fan | 2011 | Neural Regeneration Research2011，6，23： | 1 |
| 9 | IKK/NF-r,B signaling: balancing life and death-a new approach to cancer therapy显示文摘 | LUO Junli KAMATA H KARIN M | 2005 | Journal of Clinical Investigation2005，115，10： | 1 |
| 10 | Slurry-like hybrid electrolyte with high lithium-ion transference number for dendrite-free lithium metal anode显示文摘Lithium metal anode is regarded as the ultimate choice for next-generation energy storage systems,due to the lowest negative electrochemical potential and super high theoretical specific capacity.However,the growth of lithium dendrite during the cycling process is still one of the most critical bottlenecks for its application.In this work,a slurry-like hybrid electrolyte is proposed towards the application for lithium metal anode,which is composed of a liquid electrolyte part and a nanometric silane-Al2O3 particle part.The hybrid electrolyte shows high ionic conductivity(3.89×10-3 S cm-1 at 25℃)and lithium-ion transference number(0.88).Especially,the resistance of hybrid electrolyte decreases compared to that of liquid electrolyte,while the viscosity of hybrid electrolyte increases.It is demonstrated that the hybrid electrolyte can effectively suppress the growth of lithium dendrite.Stable cycling of Li/Li cells at a current density up to 1 mA cm-2 is possible.The hybrid electrolyte helps to uniform the lithium ion flux inside the battery and partly comes from the formation of a rigid and highly conductive hybrid interfacial layer on the surface of lithium metal.This work not only provides a fresh way to stabilize lithium metal anode but also sheds light on further research for electrolyte optimization and design of lithium metal battery system. | Hewei Xu Ying He Zibo Zhang Junli Shi Pingying Liu Ziqi Tian Kan Luo Xiaozhe Zhang Suzhe Liang Zhaoping Liu | 2020 | Journal of Energy Chemistry2020，29，9： | 1 |
| 11 | ARG1 functions as a tumor suppressor in breast cancer显示文摘ArginaseⅠ(ARG1)is a cytosolic enzyme that catalyzes the hydrolysis of Larginine to L-ornithine and urea.The association of ARG1 with cancer has mostly been focused on the ARG1 released by tumor-associated myeloid cells in tumor microenvironment.However,the role of ARG1 expressed in cancer cells is unclear.Here,we showed that the expression of ARG1 in human breast can-cer(BC)is related to a good prognosis in BC patients.Overexpression of ARG1 suppresses BC cell proliferation and migration in vitro and xenograft tumor growth and development in mouse models.Furthermore,ARG1 expression down-regulates the expression of p-AKT,leading to the de-activation of AKT signal pathway in BC cells.Thus,our results established that in contrast to the role of ARG1 released from tumor-associated myeloid cells in tumor microenvironment that promotes tumor immune escape,ARG1 expressed in BC cells suppresses AKT signaling pathway and functions as a tumor suppressor. | Zhengnan Ming Zizheng Zou Kaimei Cai Yi Xu Xueyan Chen Wenjun Yi Junli Luo Zhiyong Luo | 2020 | Acta Biochimica et Biophysica Sinica2020，52，11： | 0 |
| 12 | Short‐time wind speed prediction based on Legendre multi‐wavelet neural network显示文摘As one of the most widespread renewable energy sources,wind energy is now an important part of the power system.Accurate and appropriate wind speed forecasting has an essential impact on wind energy utilisation.However,due to the stochastic and un-certain nature of wind energy,more accurate forecasting is necessary for its more stable and safer utilisation.This paper proposes a Legendre multiwavelet‐based neural network model for non‐linear wind speed prediction.It combines the excellent properties of Legendre multi‐wavelets with the self‐learning capability of neural networks,which has rigorous mathematical theory support.It learns input‐output data pairs and shares weights within divided subintervals,which can greatly reduce computing costs.We explore the effectiveness of Legendre multi‐wavelets as an activation function.Mean-while,it is successfully being applied to wind speed prediction.In addition,the appli-cation of Legendre multi‐wavelet neural networks in a hybrid model in decomposition‐reconstruction mode to wind speed prediction problems is also discussed.Numerical results on real data sets show that the proposed model is able to achieve optimal per-formance and high prediction accuracy.In particular,the model shows a more stable performance in multi‐step prediction,illustrating its superiority. | Xiaoyang Zheng Dongqing Jia Zhihan Lv Chengyou Luo Junli Zhao Zeyu Ye | 2023 | CAAI Transactions on Intelligence Technology2023，8，3： | 0 |
| 13 | PRPF19 promotes tongue cancer growth and chemoradiotherapy resistance显示文摘Pre-mRNA processing factor 19(PRPF19)is a multifaceted protein and participates in DNA damage response and pre-mRNA processing.The role of PRPF19 in cancer is unclear.Here,we report that the expression of PRPF19 in human tongue cancer is associated with unfavorable prognosis.Overexpression of PRPF19 promotes while knockdown of PRPF19 inhibits tongue cancer cell migration,proliferation,and tumor growth.Overexpression of PRPF19 increases the resistance of tongue cancer cells to radiation and cisplatin treatment.Furthermore,PRPF19 regulates the expression of solute carrier family 40 member 1(SLC40A1)and mono-ADP ribosylhydrolase 2(MACROD2),knockdown of SLC40A1 or MACROD2 decreases the sensitivity of tongue cancer cells to radiation and cisplatin treatment.Thus,our results establish a key role of PRPF19 in tongue cancer growth and chemoradiotherapy resistance,targeting PRPF19 would be an effective therapeutic strategy for tongue cancer,especially for those resistant to chemoradiotherapy. | Yihong He Changhao Huang Kaimei Cai Pei Liu Xueyan Chen Yi Xu Zhengnan Ming Qingqing Liu Qiongxuan Xie Xue Xia Yangqing Sun Junli Luo Rui Wei | 2021 | Acta Biochimica et Biophysica Sinica2021，53，7： | 0 |
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