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| 1 | Isolation of circulating tumor cells in non-small-cell-lung-cancer patients using a multi-flow microfluidic channel显示文摘Circulating tumor cells(CTCs)carry a wealth of information on primary and metastatic tumors critical for precise cancer detection,monitoring,and treatment.Numerous microfluidic platforms have been developed in the past few years to capture these rare cells in patient bloodstream for deciphering the critical information needed.However,the practical need for a high-quality method of CTC isolation remains to be met.Herein,we demonstrate a novel multi-flow microfluidic device that is able to sensitively provide high purity(>87%)of separation outcome without labeling.Our device is constructed and configured based on the phenomenal effect of size-dependent inertial migration.The recovery rate of>93%has been achieved using spiked cancer cells at clinically relevant concentrations(10 cells per 5 mL and above).We have also successfully detected CTCs from 6 out of 8 non-small-cell-lung-cancer(NSCLC)patients,while none for 5 healthy control subjects.With these results,we envision our approach is a promising alternative for reliable CTC capture,and thus for facilitating the progress of extracting information from CTCs to personalize treatment strategies for solid tumor patients. | Jian Zhou Arutha Kulasinghe Amanda Bogseth Ken O’Byrne Chamindie Punyadeera Ian Papautsky | 2019 | Microsystems & Nanoengineering2019，5，1： | 9 |
| 2 | Anticancer effect of linalool via cancer-specific hydroxyl radical generation in human colon cancer显示文摘AIM To investigate the anticancer mechanisms of the monoterpenoid alcohol linalool in human colon cancer cells.METHODS The cytotoxic effect of linalool on the human colon cancer cell lines and a human fibroblast cell line was examined using the WST-8 assay. The apoptosisinducing effect of linalool was measured using the terminal deoxynucleotidyl transferase d UTP nickend labeling assay and flow cytometry with Annexin V. Oxidative stress was investigated by staining for diphenyl-1-pyrenylphosphine, which is a cellular lipid peroxidation marker, and electron spin resonance spectroscopy. Sixteen SCID mice xenografted with human cancer cells were randomized into 3 groups for in vivo analysis: control and low-dose and high-dose linalool groups. The control group was administered tap water orally every 3 d. The linalool treatment groups were administered 100 or 200 μg/kg linalool solution orally for the same period. All mice were sacrificed under anesthesia 21 d after tumor inoculation, and tumors and organs were collected for immunohistochemistry using an anti-4-hydroxynonenal antibody. Tumor weights were measured and compared between groups. RESULTS Linalool induced apoptosis of cancer cells in vitro, following the cancer-specific induction of oxidative stress, which was measured based on spontaneous hydroxyl radical production and delayed lipid peroxidation. Mice in the high-dose linalool group exhibited a 55% reduction in mean xenograft tumor weight compared with mice in the control group(P < 0.05). In addition, tumor-specific lipid peroxidation was observed in the in vivo model.CONCLUSION Linalool exhibited an anticancer effect via cancerspecific oxidative stress, and this agent has potential for application in colon cancer therapy. | Kenichi Iwasaki Yun-Wen Zheng Soichiro Murata Hiromu Ito Ken Nakayama Tomohiro Kurokawa Naoki Sano Takeshi Nowatari Myra O Villareal Yumiko N Nagano Hiroko Isoda Hirofumi Matsui Nobuhiro Ohkohchi | 2016 | World Journal of Gastroenterology2016，22，44： | 5 |
| 3 | Comparison of paralytic shellfish poisoning toxin between carnivorous crabs (Telmessus acutidens and Charybdis japonica) and their prey mussel (Mytilus galloprovincialis) in an inshore food chain显示文摘 | HIROSHI O TSUNEO F KEN S | 2004 | Toxicon2004，43，： | 1 |
| 4 | Enhancement of visible light-induced hydrophilicity on nitrogen and sulfur-codoped TiO2 thin films 显示文摘 | W S Yuka O Ken H Kazuhito | 2009 | Vacuum2009，83，3： | 1 |
| 5 | Drastic SOxremoval and influences of various factors in O2/CO2pulverized coal combustion system显示文摘 | Hao L Shiro K Ken O | 2001 | Energy&Fuels2001，15，2： | 1 |
| 6 | Nitric oxide activity is deficient in spasm arteries of patients with coronary spastic angina 显示文摘 | KUGIYAMA K,YASUE H,KEN O, | 1996 | Circulation1996，94，3： | 1 |
| 7 | Effect of temperature on ascorbate peroxidase activity and flowering of Arabidopsis thalianaecotypes under different light conditions显示文摘 | Shubhangi D L Ken'ichi O Ayumi T | 2003 | J Plant Phys2003，160，： | 1 |
| 8 | Effect of photosensitizer and hydrogen peroxide on desulfurization of light oil by photochemical reaction and liquid-liquid extraction显示文摘 | Takayuki H Yasuhiro S Ken O | 1997 | Ind Eng Chem Res1997，36，： | 1 |
| 9 | Mechanism of the conversion of xanthine dehydrogenase to xanthine oxidase:identification of the two cysteine disulfide bonds and crystal structure of a non-convertible rat liver xanthine dehydrogenase mutant显示文摘 | Tomoko N Ken O Yuko K | 2005 | J Biol Chem2005，280，24： | 1 |
| 10 | Investigation of the clinical effect of large volume leukocytapheresis on methotrexate - resistant rheumatoid arthritis 显示文摘 | SHIN O KEN Y KAZUO K | 2006 | Ther Apher Dial2006，10，5： | 1 |
| 11 | Effect of photosensitizer and hydrogen peroxide on desulfurization of light oil by photochemical reaction and liquid-liquid extraction显示文摘 | TAKAYUKI H YASUHIRO S KEN O ISAO K | 1997 | Ind Eng Chem Res1997，36，3： | 1 |
| 12 | Effect of casein genotype on milk performance in Japanese shorthorn cow 显示文摘 | Nakayarna O Mistsyji O Ken N | 1995 | Animal Science and Technology1995，66，8： | 1 |
| 13 | Elevated pyruvate, orthophosphate dikinase (PPDK) activity alters carbon metabolism in C3 transgenic potatoes with a Ca maize PPDK gene显示文摘 | KEN I YASUNOBU O TERUO I | 1998 | Physiol Plantarum1998，103，3： | 1 |
| 14 | Extracellular matrix gene regulation 显示文摘 | KEN O LINDA J S | 2004 | Clin Orthop2004，427，5： | 1 |
| 15 | A preliminary model for predicting heavy metal contaminant loading from an urban catchment显示文摘 | Yi Y Ken H Carolyn O | 2001 | The Science of the Total Environment2001，266，： | 1 |
| 16 | Early-life-stage toxicity in offspring from exposed parent medaka, oryzias latipes, to mixtures of tributyltin and polychlorinated biphenyls显示文摘 | Kei N Yuji O Ken N | 2005 | Environ Toxicol Pharmacol2005，24，3： | 1 |
| 17 | Twin-wire GMAW : Process Characteristics and Applications 显示文摘 | KEN M STEPHEN B GUNBIYI T E B O | 1999 | Welding Journal1999，78，05： | 1 |
| 18 | Contributions of two nuclear localization signals of influenza A virus nucleoprotein to viral replication显示文摘 | MAKOTO O KEN F YUKIKO M | 2007 | J Virol2007，81，1： | 1 |
| 19 | Scintigraphic evaluation of osteoblastic activity in extraction rockets treated with platelet-rich fibrin显示文摘 | G(U)RB(U)ZER B PIKD(O)KEN L TUNALI M | 2010 | J Oral Maxillofac Surg2010，68，5： | 1 |
| 20 | Are contents of rubisco, soluble protein and nitrogen in flag leaves of rice controlled by the same genetics显示文摘 | Ken I Noriko K Kiyomi O | 2001 | Journal of Experimental Botany2001，52，362： | 1 |
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