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| 1 | INTRACELLULAR MANIPULATION BY FEMTOSECOND LASERS:REVIEW显示文摘Multiphoton absorption of femtosecond laser pulses focused through an objective with high numerical aperture(NA)can be used to image and manipulate cellular and intracellular objects.This review highlights recent advances in intracellular manipulation,including nanosurgery and labeling in living cells with femtosecond lasers. | WATARU WATANABE SACHIHIIRO MATSUNAGA KIICHI FUKUI KAZUYOSHI ITOH | 2009 | Journal of Innovative Optical Health Sciences2009，2，1： | 2 |
| 2 | Repetitive sequences: cause for variation in genome size and chromosome morphology in the genus Oryza显示文摘 | Sakurako Uozu Hiroshi Ikehashi Nobuko Ohmido Hisako Ohtsubo Eiichi Ohtsubo Kiichi Fukui | 1997 | Plant Molecular Biology1997，，6： | 1 |
| 3 | Physical mapping of the 5S ribosomal RNA genes on rice chromosome 11显示文摘 | Yasuko Kamisugi Shigeki Nakayama Reiko Nakajima Hisako Ohtsubo Eiichi Ohtsubo Kiichi Fukui | 1994 | MGG Molecular & General Genetics1994，，2： | 1 |
| 4 | Physical mapping of unique nucleotide sequences on identified rice chromosomes显示文摘 | Nobuko Ohmido Yukio Akiyama Kiichi Fukui | 1998 | Plant Molecular Biology1998，，6： | 1 |
| 5 | Chromosomal regions controlling anther culturability in rice (Oryza sativa L.)显示文摘 | Masumi Yamagishi Motoyasu Otani Mariko Higashi Yoshimichi Fukuta Kiichi Fukui Takiko Shimada | 1998 | Euphytica1998，，2： | 1 |
| 6 | Genome and Chromosome Dimensions of Lotus japonicus显示文摘 | Mikako Ito Junko Miyamoto Yumiko Mori Satoru Fujimoto Toshiki Uchiumi Mikiko Abe Akihiro Suzuki Satoshi Tabata Kiichi Fukui | 2000 | Journal of Plant Research2000，，4： | 1 |
| 7 | Trends in site-number change of rDNA loci during polyploid evolution in Sanguisorba (Rosaceae)显示文摘 | Misako Mishima Nobuko Ohmido Kiichi Fukui Tetsukazu Yahara | 2002 | Chromosoma2002，，8： | 1 |
| 8 | Chromosomal location and reorganization of the 45S and 5S rDNA in theBrachyscome lineariloba complex (Asteraceae)显示文摘 | Junko Adachi Kuniaki Watanabe Kiichi Fukui Nobuko Ohmido Keiko Kosuge | 1997 | Journal of Plant Research1997，，3： | 1 |
| 9 | Quantitative chromosome map of the polyploid Saccharum spontaneum by multicolor fluorescence in situ hybridization and imaging methods显示文摘 | Sen Ha Paul H. Moore Don Heinz Seiji Kato Nobuko Ohmido Kiichi Fukui | 1999 | Plant Molecular Biology1999，，6： | 1 |
| 10 | Applications of image analysis in plant chromosome and chromatin structure study显示文摘Background:The use of image analysis to understand the structure of chromosome and chromatin is critical to the study of genetic evolution and diversification.Furthermore,a detailed chromosome map and the structure of chromatin in the nucleus may contribute to the plant breeding and the study of fundamental biology and genetics in organisms.Results:In plants with a fully annotated genome project,such as the Leguminosae species,the integration of genetic information,including DNA sequence data,a linkage map,and the cytological quantitative chromosome map could further improve their genetic value.The numerical parameters of chromocenters in 3D can provide useful genetic information for phylogenetic studies of plant diversity and heterochromatic markers whose epigenetic changes may explain the developmental and environmental changes in the plant genome.Extended DNA fibers combined with fluorescence in situ hybridization revealed the highest spatial resolution of the obtained genome structure.Moreover,image analysis at the nano-scale level using a helium ion microscope revealed the surface structure of chromatin,which consists of chromatin fibers compacted into plant chromosomes.Conclusions:The studies described in this review sought to measure and evaluate chromosome and chromatin using the image analysis method,which may reduce measurement time and improve resolution.Further,we discussed the development of an effective image analysis to evaluate the structure of chromosome and chromatin.An effective application study of cell biology and the genetics of plants using image analysis methods is expected to be a major propeller in the development of new applications. | Nobuko Ohmido Astari Dwiranti Seiji Kato Kiichi Fukui | 2022 | Quantitative Biology2022，10，3： | 0 |
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