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| 1 | 不同提取方法山苍子油的化学成分与抗氧化活性分析显示文摘【目的】比较不同提取方法对山苍子油提取效率与抗氧化活性的影响,通过GC-MS分析,研究山苍子油成分构成与抗氧化活性的内在关联,分析山苍子油抗氧化活性的主要物质基础。【方法】采用液液萃取(分别加以超声波与磁化辅助处理)、二氧化碳超临界萃取以及水蒸气蒸馏法提取山苍子油,DPPH法比较不同提取方式对山苍子油抗氧化活力的影响,GC-MS分析山苍子油的主要成分,并以1,4-二溴苯为内标,采用面积归化法解析主要成分的相对含量。【结果】山苍子油具有显著的抗氧化活性。液液萃取法的提取效率与山苍子油抗氧化活性最高,超声波辅助处理油得率最高,为26.3%,磁化辅助处理抗氧化活性最优,达到31.22 mg·mL^-1(IC50),二氧化碳超临界萃取法次之,抗氧化活性为56.95 mg·mL^-1(IC50),水蒸气蒸馏法抗氧化活性最弱,为64.95 mg·mL^-1(IC50);GC-MS分析检测出122种以上的化合物,包括12种脂肪酸,16种萜烯,18种含氧萜烯以及其他微量化合物包括烯烃、醇、酮类与烷烃等。GC-MS分析结果表明:山苍子干果主要成分为饱和脂肪酸(月桂酸等)、不饱和脂肪酸(9,12-十八碳二烯酸、9-十八碳烯酸等)以及α蒎烯、β蒎烯、柠檬烯等萜烯、氧化萜烯类物质。液液萃取法得到的山苍子油脂肪酸含量(53.51%-66.61%)明显高于二氧化碳超临界萃取(17.64%)与水蒸气蒸馏法(8.1%),而二氧化碳超临界萃取与水蒸气蒸馏法得到的萜烯、氧化萜烯类含量分别达到29.37%和17.69%,明显高于液液萃取法(6.49%-9.75%)。【结论】磁化辅助处理有利于抗氧化活性物质的提取,且不饱和脂肪酸的含量最高;超声波辅助处理可以提升油的得率;水蒸气蒸馏法和超临界流体萃取法更有利于萜烯类化合物的获取;联系到水蒸气蒸馏法得到的山苍子油富含萜烯化合物却显示较低的抗氧化活性(64.95 mg·mL^-1),色谱级的柠檬醛抗氧化活性最低(74.33 mg·mL^-1),因此推测脂肪酸成分(特别是不饱和脂肪酸)是山苍子油抗氧化活性的主要物质基础。 | 李芳 Yaru Song Howard Holness Kevin O’Shea | 2015 | 林业科学2015，51，3： | 14 |
| 2 | Effect of sample size on the response of DEM samples with a realistic grading显示文摘This paper shows that for DEM simulations of triaxial tests using samples with a grading that is representative of a real soil,the sample size significantly influences the observed material response.Four DEM samples with identical initial states were produced:three cylindrical samples bounded by rigid walls and one bounded by a cubical periodic cell.When subjected to triaxial loading,the samples with rigid boundaries were more dilative,stiffer and reached a higher peak stress ratio than the sample enclosed by periodic boundaries.For the rigid-wall samples,dilatancy increased and stiffness decreased with increasing sample size.The periodic sample was effectively homogeneous.The void ratio increased and the contact density decreased close to the rigid walls.This heterogeneity reduced with increasing sample size.The positions of the critical state lines(CSLs) of the overall response in e-log p' space were sensitive to the sample size,although no difference was observed between their slopes.The critical states of the interior regions of the rigid-wall-bounded samples approached that of the homogeneous periodic sample with increasing sample size.The ultimate strength of the material at the critical state is independent of sample size. | Xin Huang Kevin J.Hanley Catherine O'Sullivan Fiona C.Y.Kwok | 2014 | Particuology2014，12，4： | 12 |
| 3 | Cardiac biomarkers in pediatric heart disease:A state of art review显示文摘Every year there are more than 11000 hospitalizations related to heart failure in children resulting in significant morbidity and mortality.Over the last two decades,our understanding,diagnosis and management of pediatric heart failure is evolving but our ability to prognosticate outcomes in pediatric heart acute heart failure is extremely limited due to lack of data.In adult heart failure patients,the role of cardiac biomarkers has exponentially increased over the last two decades.Current guidelines for management of heart failure emphasize the role of cardiac biomarkers in diagnosis,management and prognostication of heart failure.It is also noteworthy that these biomarkers reflect important biological processes that also open up the possibility of therapeutic targets.There is however,a significant gap present in the pediatric population with regards to biomarkers in pediatric heart failure.Here,we seek to review available data regarding cardiac biomarkers in the pediatric population and also explore some of the emerging biomarkers from adult literature that may be pertinent to pediatric heart failure. | Benedict A Fernandes Kevin O Maher Shriprasad R Deshpande | 2016 | World Journal of Cardiology2016，8，12： | 6 |
| 4 | Implementation of rotational resistance models' A critical appraisal显示文摘 | Xin Huang Kevin J. Hanley Catherine O'Sullivan Chung-Yee Kwok | 2017 | Particuology2017，15，5： | 4 |
| 5 | Chronic pancreatitis:A diagnostic dilemma显示文摘Typical clinical symptoms of chronic pancreatitis are vague and non-specific and therefore diagnostic tests are required, none of which provide absolute diagnostic certainly, especially in the early stages of disease. Recently-published guidelines bring much needed structure to the diagnostic work-up of patients with suspected chronic pancreatitis. In addition, novel diagnostic modalities bring promise for the future. The assessment and diagnosis of pancreatic exocrine insufficiency remains challenging and this review contests the accepted perspective that steatorrhea only occurs with > 90% destruction of the gland. | Sinead N Duggan Hazel M NíChonchubhair Oladapo Lawal Donal B O’Connor Kevin C Conlon | 2016 | World Journal of Gastroenterology2016，22，7： | 4 |
| 6 | 手术中知晓事件的新分级标准显示文摘背景有外显记忆的手术中知晓事件的发生率仅为1/1000—2/1000。因其较罕见,为了更好的了解手术中知晓事件及其后遗效直,需要对大批量的研究数据进行统计。因此,对其进行标准描述和表达将很有价值。方法本研究创建了一种新的手术中知晓事件评估标准:0级:未发生手术中知晓;1级:仅存在听觉感知;2级:感知触觉(比如手术操作、气管插管);3级:感知痛觉;4级:感知麻痹(比如感到不能活动、说话或呼吸);5级:感知麻痹及痛觉。患者主述有恐惧、焦虑、窒息、末日感、濒死感及其他相关显性描述,另行附加“D”(distress[痛苦、悲伤])。本研究回顾了15项详细提供手术中知晓报告信息的研究。来自3个研究并创建了该项分级标准的5位麻醉医生,各自独立的评估了这些病例。另外20名相关人员(麻醉主治医生、麻醉住院医生、麻醉护士、医学生及其辅助工作人员),虽未参与该分级标准的创建,但也独立对手术中知晓事件进行了评估。用Flwiss’s κ系数评估观察者之间的一致性。结果对151例确认发生手术中知晓事件的成年病例进行数据分析。基础评估1~5级中,总体κ系数为0.851(95%可信区间CI0.847~0.856)。包括附加情感障碍“D”的评估中,总体κ系数为0.779(95%可信区间CI 0.776~0.783)。结论本研究创建了新的手术中知晓事件评估标准,在评估者间具有近乎完美的一致性,这将有助于手术中知晓事件的研究。 | George A. Mashour Roy K. Esaki Kevin K. Tremper David B. Glick Michael O'Connor Michael S. Avidan 潘鹏(译) 李文志(校) | 2009 | 麻醉与镇痛2009，，6： | 3 |
| 7 | The amazing potential of fungi:50 ways we can exploit fungi industrially显示文摘Fungi are an understudied,biotechnologically valuable group of organisms.Due to the immense range of habitats that fungi inhabit,and the consequent need to compete against a diverse array of other fungi,bacteria,and animals,fungi have developed numerous survival mechanisms.The unique attributes of fungi thus herald great promise for their application in biotechnology and industry.Moreover,fungi can be grown with relative ease,making production at scale viable.The search for fungal biodiversity,and the construction of a living fungi collection,both have incredible economic potential in locating organisms with novel industrial uses that will lead to novel products.This manuscript reviews fifty ways in which fungi can potentially be utilized as biotechnology.We provide notes and examples for each potential exploitation and give examples from our own work and the work of other notable researchers.We also provide a flow chart that can be used to convince funding bodies of the importance of fungi for biotechnological research and as potential products.Fungi have provided the world with penicillin,lovastatin,and other globally significant medicines,and they remain an untapped resource with enormous industrial potential. | Kevin D.Hyde Jianchu Xu Sylvie Rapior Rajesh Jeewon Saisamorn Lumyong Allen Grace T.Niego Pranami D.Abeywickrama Janith V.S.Aluthmuhandiram Rashika S.Brahamanage Siraprapa Brooks Amornrat Chaiyasen K.W.Thilini Chethana Putarak Chomnunti Clara Chepkirui Boontiya Chuankid Nimali I.de Silva Mingkwan Doilom Craig Faulds Eleni Gentekaki Venkat Gopalan Pattana Kakumyan Dulanjalee Harishchandra Hridya Hemachandran Sinang Hongsanan Anuruddha Karunarathna Samantha C.Karunarathna Sehroon Khan Jaturong Kumla Ruvishika S.Jayawardena Jian-Kui Liu Ningguo Liu Thatsanee Luangharn Allan Patrick G.Macabeo Diana S.Marasinghe Dan Meeks Peter E.Mortimer Peter Mueller Sadia Nadir Karaba N.Nataraja Sureeporn Nontachaiyapoom Meghan O’Brien Watsana Penkhrue Chayanard Phukhamsakda Uma Shaanker Ramanan Achala R.Rathnayaka Resurreccion B.Sadaba Birthe Sandargo Binu C.Samarakoon Danushka S.Tennakoon Ramamoorthy Siva Wasan Sriprom T.S.Suryanarayanan Kanaporn Sujarit Nakarin Suwannarach Thitipone Suwunwong Benjarong Thongbai Naritsada Thongklang Deping Wei S.Nuwanthika Wijesinghe Jake Winiski Jiye Yan Erandi Yasanthika Marc Stadler | 2019 | Fungal Diversity2019，，4： | 3 |
| 8 | Epistaxis in end stage liver disease masquerading as severe upper gastrointestinal hemorrhage显示文摘AIM:To describe the prevalence,diagnosis,treatment,and outcomes of end stage liver disease(ESLD) patients with severe epistaxis thought to be severe upper gastrointestinal hemorrhage(UGIH).METHODS:This observational single center study included all consecutive patients with ESLD and epistaxis identified from consecutive subjects hospitalized with suspected UGIH and prospectively enrolled in our databases of severe UGIH between 1998 and 2011.RESULTS:A total of 1249 patients were registered for severe UGIH in the data basis,461(36.9%) were cirrhotics. Epistaxis rather than UGIH was the bleeding source in 20 patients. All patients had severe coagulopathy. Epistaxis was initially controlled in all cases. Fifteen(75%) subjects required posterior nasal packing and 2(10%) embolization in addition to correction of coagulopathy. Five(25%) patients died in the hospital,12(60%) received orthotopic liver transplantation(OLT),and 3(15%) were discharged without OLT. The mortality rate was 63% in patients without OLT.CONCLUSION:Severe epistaxis in patients with ESLD is(1) a diagnosis of exclusion that requires upper endoscopy to exclude severe UGIH;and(2) associated with a high mortality rate in patients not receiving OLT. | Marine Camus Dennis M Jensen Jason D Matthews Gordon V Ohning Thomas O Kovacs Rome Jutabha Kevin A Ghassemi Gustavo A Machicado Gareth S Dulai | 2014 | World Journal of Gastroenterology2014，20，38： | 3 |
| 9 | 人参减少 γ射线照射的淋巴细胞微核量(英文)显示文摘目的 评价中国人参对减少辐射诱导的人周围血 G0 期淋巴细胞 (PBL)微核量的作用。方法 采用细胞因子阻滞的微核法检测健康志愿者的血标本。体外 1 37Cs照射之前 ,每份血标本的单个核细胞培养以不同浓度 (0~ 2 0 0 0μg· m l- 1 )的人参根水提取物孵育 2 4 h。结果 (1)在 0 Gy时 ,每 10 0 0个双核 (BN )细胞的微核量是 11.3± 2 .7,人参水提取物≥ 5 0 0μg· ml- 1 时微核量下降 ,但微核量和人参水提取物的浓度之间的差异是显著的。 (2 )单独射线照射显著增加微核量 ,当暴露于 1Gy的照射时 ,随着培养介质中人参水提取物浓度的增加 ,微核量呈显著的线性降低 (R2 =0 .0 5 ,P =0 .0 0 2 ) ;对暴露于 1Gy辐射诱导的微核量 ,人参水提取物浓度在 15 0 0μg· m l- 1 时减少 4 6 .6 % ,浓度在 2 0 0 0μg·m l- 1时减少 5 7.6 % ;对暴露于 2 Gy辐射诱导的微核量的减少呈更强的线性相关 (R2 =0 .7,P=0 .0 0 0 1) ;(3)微核数据分析的结果呈现出与 Poisson模型相关的过度离散趋势。结果表明 :(1)人参水提取物浓度达 2 0 0 0 μg· ml- 1时 ,对 PBL 细胞毒作用无明显影响 ;(2 )人参水提取物浓度在 5 0 0 μg· ml- 1和 2 0 0 0 μg· ml- 1之间时 ,对抗 1 37Cs照射诱导的 MN发挥保护作用。 | 李同光 Ron R Allison Kevin F O'Brien Prabhaker G Khazanie Larry J Dobbs Jr. Peter J Kragel | 2004 | 新乡医学院学报2004，21，3： | 3 |
| 10 | Fabrication of Noble metal-semiconductor hybrid nanostructures using phase transfer显示文摘 | Tanushree Bala Ajay Singh Ambarish Sanyal Catriona O'Sullivan Fathima Laffir Claudia Coughlan Kevin M. Ryan | 2013 | Nano Research2013，6，2： | 3 |
| 11 | 全球化与中国经济发展显示文摘从中国的崛起及对世界经济的影响、中国对全球化的认识和接纳、全球化对中国的影响等方面,探讨中国对外开放所产生的双重影响。 | Kevin O Brien 何小琴 | 2002 | 江西财经大学学报2002，，1： | 3 |
| 12 | Pedicle Morphology In Thoracic Adolescent Idiopathic Scoliosis: Is Pedicle Fixation an Anatomically Viable Technique?显示文摘 | Michael F O’Brien Lawrence G Lenke Steven Mardjetko Thomas G Lowe Yinong Kong Kevin Eck David Smith | 2000 | Spine2000，，18： | 2 |
| 13 | Lactobacillus and bifidobacterium in irritable bowel syndrome: Symptom responses and relationship to cytokine profiles显示文摘 | Liam O’Mahony Jane McCarthy Peter Kelly George Hurley Fangyi Luo Kersang Chen Gerald C. O’Sullivan Barry Kiely J. Kevin Collins Fergus Shanahan Eamonn M.M. Quigley | 2005 | Gastroenterology2005，，3： | 2 |
| 14 | C-reactive protein and radiographic findings of lower respiratory tract infection in infants显示文摘AIM To evaluate the association between C-reactive protein(CRP) and radiological evidence of lower respiratory tract infection(LRTI) in infants.METHODS All patients aged less than 4 years who presented with suspected lower respiratory tract infection,who received a peri-presentation chest radiograph and CRP blood measurement over an 18-mo period were included in the study.Age,gender,source of referral,CRP,white cell count,neutrophil count along with the patients' symptoms and radiologist's report were recorded.RESULTS Three hundred and eleven patients met the inclusioncriteria.Abnormal chest radiographs were more common in patients with elevated CRP levels(P < 0.01).Radiologic signs of LRTI were identified in 73.7% of chest radiographs when a patient had a CRP level between 50-99 mg/L.CRP levels were a better predictor of positive chest radiograph findings for those aged greater than I year compared to those 1 year or less.CONCLUSION CRP may be used in patients with suspected LRTI diagnosis to select those who are likely to have positive findings on chest radiograph,thus reducing unnecessary chest radiographs. | Maria Twomey Hannah Fleming Fiachra Moloney Kevin P Murphy Lee Crush Siobhan B O’Neill Oisin Flanagan Karl James Conor Bogue Owen J O’Connor Michael M Maher | 2017 | World Journal of Radiology2017，9，4： | 2 |
| 15 | 4号染色体短臂微卫星多态性与鼻咽癌相关性的研究显示文摘鼻咽癌(NPC)是一种多因素复杂疾病。其发病过程涉及EB病毒慢性感染、环境致癌因素及宿主基因之间的相互作用。在这一过程中,那些宿主基因在EB病毒感染及鼻咽癌的发生发展中起了关键作用仍不清楚。本研究的目的是发现与鼻咽癌发生发展中两个关键步骤相关的遗传变异,即EB病毒持续性感染鼻咽部上皮细胞和鼻咽癌的形成。我们在广西梧州市及苍梧县鼻咽癌高发区收集汉族鼻咽癌患者350例、EB病毒壳抗原IgA抗体阳性者(IgA/VCA+)288例和EB病毒壳抗原IgA抗体阴性者(IgA/VCA—)346例。对先前鼻咽癌家系研究显示的鼻咽癌易感区4号染色体短臂(4p15.1~q12)进行了微卫星精细扫描,在18Mb的范围内选择34个微卫星标记,包括319个等位基因,对其进行基因分型。比较分析NPC组和IgA/VCA+组等位基因频率结果显示,9个等位基因与鼻咽癌呈相关,其中5个为易感等位基因(OR=1.51~5.36,P=0.01~0.03),4个为限制性等位基因(OR值为0.3~0.71,P值为0.02~0.045)。比较分析IgA/VCA+组和IgA/VCA-组及比较所有IgA/VCA+者(包括NPC患者)和IgA/VCA-者等位基因频率的结果显示,12个等位基因与EB病毒壳抗原IgA抗体持续存在相关,其中3个在两组比较中均呈显著相关。等位基因D4S3241~136(P=0.004,OR=1.9,95%CI=1.2~3.0)和D4S3347-213(P=0.001,OR=1.6,95%CI=1.2~2.1)可增加EB病毒IgA/VCA抗体形成的危险,为易感基因;而等位基因D4S174.202(P=0.001,OR=0.5,95%CI=0.3~0.7)可限制IgA/VCA抗体的形成。但上述结果经多因素比较校正后,均失去相关性。结果不能确定该区域与非家族性鼻咽癌的形成相关,但本研究却提供了进一步发现鼻咽癌相关基因的研究模式。有关4号染色体短臂与EB病毒慢性持续感染及鼻咽癌的形成仍值得进一步深入研究。 | 郭秀婵 O’BRIEN Stephen J WINKLER Cheryl SCOTT Kevin HUTCHESON Holli DAVID Victor KESSING Bailey 郑裕明 廖建 刘彦 GUY de The 曾毅 | 2006 | 遗传2006，28，7： | 2 |
| 16 | Multidisciplinary approach for multifocal, bilobar hepatocellular carcinoma: A case report and literature review显示文摘BACKGROUND Hepatocellular carcinoma(HCC) is the second most lethal malignancy worldwide. There has been virtually no change in the survivability of HCC in spite of improvement in therapies. Surgery is considered the ideal first, curative intervention, however most patients present in advanced stages with unresectable disease. Therefore, systemic and liver-directed non-operative therapies are initially offered to downstage the disease. To ensure optimal management, a multidisciplinary team approach is often warranted. Our case highlights the benefits of a multidisciplinary approach in a young woman with multifocal, bilobar HCC.CASE SUMMARY A 36-year-old Chinese woman with untreated hepatitis B was found to have large bilobar HCC during work up for abdominal pain. Her initial serum alphafetoprotein was significantly elevated to 311136 ng/mL. Computed tomography scan demonstrated bulky bilobar liver masses, consistent with intermediate stage HCC, Barcelona Clinic Liver Cancer Stage B. Her case was discussed and a personalized care plan was developed at the Multidisciplinary Center for Advanced Minimally Invasive Liver Oncologic Therapies at the University of Washington. She initially underwent bilobar transarterial chemoembolization with partial response of the left lobar tumor. Salvage hypofractionated proton beam radiation therapy was delivered to the right lobe followed by two additional transarterial chemoembolizations to the left lobe with good response.Finally, to remove left lobar residual disease, she was taken to the operating roomfor a left hepatectomy eleven months after her initial presentation. She continues to be without evidence of disease.CONCLUSION Coordinating the multiple HCC treatment modalities is complex and our case highlights the benefits of a multidisciplinary approach. | Kevin P Labadie Stephanie K Schaub Derek Khorsand Guy Johnson Smith Apisarnthanarax James O Park | 2019 | World Journal of Hepatology2019，11，1： | 2 |
| 17 | Discrete element modelling of the quasi-static uniaxial compression of individual infant formula agglomerates显示文摘Infant formula is usually produced in an agglomerated powder form. These agglomerates are subjected to many transient forces following their manufacture. These can be difficult to quantify experimentally because of their small magnitudes and short durations. Numerical models have the potential to address this gap in the experimental data. The objective of the research described here was to calibrate a discrete element model for these agglomerates using experimental data obtained for quasi-static loading, and to use this model to study the mechanics of the particle response in detail. The Taguchi method was previously proposed as a viable calibration approach for discrete element models. In this work, the method was assessed for calibration of the model parameters (e.g., bond stiffnesses and strengths) considering three responses: the force at failure, strain at failure and agglomerate stiffness. The Weibull moduli for the simulation results and the experimental data were almost identical following calibration and the 37% characteristic stresses were similar. An analysis of the energy terms in the model provided useful insight into the model response. The bond energy and the normal force exerted on the platens were strongly correlated, and bond breakage events coincided with the highest energy dissipation rates. | Kevin J.Hanley Catherine O'Sullivan Edmond P.Byrne Kevin Cronin | 2012 | Particuology2012，10，5： | 2 |
| 18 | INTERLEUKIN-10 AND THE INTERLEUKIN-10 RECEPTOR显示文摘 | Kevin W. Moore Rene de Waal Malefyt Robert L. Coffman Anne O’Garra | 2001 | Annual Review of Immunology2001，，： | 2 |
| 19 | Effect of a fall in malaria transmission on morbidity and mortality in Kilifi, Kenya显示文摘 | Wendy P O'Meara Phillip Bejon Tabitha W Mwangi Emelda A Okiro Norbert Peshu Robert W Snow Charles RJC Newton Kevin Marsh | 2008 | The Lancet2008，，9649： | 2 |
| 20 | Effectiveness of treatment for class Ⅱ malocclusion with the Herbst or Twinblock appliance:A randomized,controlled trial显示文摘 | Kevin O'Brien Wright J Sanjie Y | 2003 | Am J Orthod Dentofac Orthod2003，124，： | 1 |
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