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| 1 | Experimental Study of the Pressure Fluctuations in a Pump Turbine at Large Partial Flow Conditions显示文摘Frequent shifts of output and operating mode require a pump turbine with excellent stability. Current researches show that large partial flow conditions in pump mode experience positive-slope phenomena with a large head drop. The pressure fluctuation at the positive slope is crucial to the pump turbine unit safety. The operating instabilities at large partial flow conditions for a pump turbine are analyzed. The hydraulic performance of a model pump turbine is tested with the pressure fluctuations measured at unstable operating points near a positive slope in the performance curve. The hydraulic performance tests show that there are two separated positive-slope regions for the pump turbine, with the flow discharge for the first positive slope from 0.85 to 0.91 times that at the maximum efficiency point. The amplitudes of the pressure fluctuations at these unstable large partial flow conditions near the first positive slope are much larger than those at stable operating condtions. A dominant frequency is measured at 0.2 times the impeller rotational frequency in the flow passage near the impeller exit, which is believed to be induced by the rotating stall in the flow passage of the wicket gates. The test results also show hysteresis with pressure fluctuations when the pump turbine is operated near the first positive slope. The hysteresis creates different pressure fluctuations for those operation points even though their flow rates and heads are similar respectively. The pressure fluctuation characteristics at large partial flow conditions obtained by the present study will be helpful for the safe operation of pumped storage units. | RAN Hongjuan LUO Xianwu ZHU Lei ZHANG Yao WANG Xin XU Hongyuan | 2012 | Chinese Journal of Mechanical Engineering2012，25，6： | 33 |
| 2 | Programmed death-1 expression is associated with the disease status in hepatitis B virus infection显示文摘AIM: To define the potential role of programmed death-1/programmed death-ligand (PD-1/PD-L) pathway in different hepatitis B virus (HBV) infection disease status; we examined the expression of PD-1 on antigen specific CD8+ T cells in peripheral blood of patients with chronic hepatitis B (CHB) and acute exacerbation of hepatitis B (AEHB) infection. METHODS: The PD-1 level on CD8+ T lymphocytes and the number of HBV specifi c CD8+ T lymphocytes in patients and healthy controls (HCs) were analyzed by staining with pentameric peptide-human leukocyte antigen2 (HLA2) complexes combined with flow cytometry. Real-time quantitative polymerase chain reaction (PCR) was used to measure the serum HBV-DNA levels. RESULTS: The level of PD-1 expression on total CD8+ T cells in CHB patients (13.86% ± 3.38%) was significantly higher than that in AEHB patients (6.80%± 2.19%, P < 0.01) and healthy individuals (4.63% ± 1.23%, P < 0.01). Compared to AEHB patients (0.81% ± 0.73%), lower frequency of HBV-specific CD8+ T cells was detected in chronic hepatitis B patients (0.37% ± 0.43%, P < 0.05). There was an inverse correlation between the strength of HBV-specific CD8+ T-cell response and the level of PD-1 expression. Besides, there was a significant positive correlation between HBV viral load and the percentage of PD-1 expression on CD8+ T cells in CHB and AEHB subjects (R = 0.541, P < 0.01). However, PD-1 expression was not associated with disease flare-ups as indicated by alanine aminotransferase (ALT) levels (R = 0.066, P > 0.05). CONCLUSION: Our results confirm previous reports that HBV specific CD8+ T-cell response in the peripheral blood is more intense in patients with AEHB than in chronic hepatitis B with persistent viral infection. Moreover, there is a negative correlation between the level of PD-1 and the intensity of virus specific CD8+T cell response. | Plan Ye Zhi-Hong Weng Shu-Ling Zhang Jian-Ao Zhang Lei Zhao Ji-Hua Dong Sheng-Hua Jie Ran Pang Rong-Hua Wei | 2008 | World Journal of Gastroenterology2008，14，28： | 25 |
| 3 | Highly efficient generation of arbitrary vector beams with tunable polarization, phase, and amplitude显示文摘We propose an efficient and robust method to generate tunable vector beams by employing a single phasetype spatial light modulator(SLM). With this method, a linearly polarized Gaussian beam can be converted into a vector beam with arbitrarily controllable polarization state, phase, and amplitude. The energy loss during the conversion is greatly reduced and depends mainly on the reflectivity of the SLM. We experimentally demonstrate that conversion efficiency of about 47% is achieved by using an SLM with reflectivity of 62%.Several typical vector beams, including cylindrical vector beams, vector beams on higher order Poincaré spheres,and arbitrary vector beams attached with phases and with tunable amplitude, are generated and verified experimentally. This method is also expected to create high-power vector beams and play important roles in optical fabrication and light trapping. | SHENG LIU SHUXIA QI YI ZHANG PENG LI DONGJING WU LEI RAN JIANLIN ZHAO | 2018 | Photonics Research2018，6，4： | 23 |
| 4 | Development of synthetic volatile attractant for male Ectropis obliqua moths显示文摘The tea geometrid Ectropis obliqua is one of the most serious leaf-feeding insect pests in tea(Camellia sinensis) in East Asia. Although several volatile chemicals emitted from tea plants have been reported to be attractive to E. obliqua moths, no synthetic attractants for E. obliqua moths have been developed. By measuring the behavioral responses of the moth to a series of chemicals in the lab, we found that a blend containing a ternary mixture containing(Z)-3-hexenal,(Z)-3-hexenyl hexanoate and benzyl alcohol clearly attracted to E. obliqua moths of both sex and that(Z)-3-hexenyl acetate could enhance the attractiveness of the ternary blend. Moreover, we found that the volatiles emitted from the plant-E. obliqua larva complex have the same attractiveness as: 1) the blend of volatiles containing the ternary mixture and 2) the blend containing(Z)-3-hexenyl acetate plus the ternary mixture to both male and female moths. In a field bioassay, more male moths were observed on traps that were baited with the blend containing(Z)-3-hexenyl acetate plus the ternary mixture than on control traps. Our study raises the tantalizing possibility that synthetic blends could be deployed as attractants for pests in the field. | SUN Xiao-ling LI Xi-wang XIN Zhao-jun HAN Juan-juan RAN Wei LEI Shu | 2016 | Journal of Integrative Agriculture2016，15，7： | 10 |
| 5 | Comparison of the performance of the CRUSADE, ACUITY-HORIZONS, and ACTION bleeding scores in ACS patients undergoing PCI: insights from a cohort of 4939 patients in China显示文摘BackgroundThe 十字军,行动和尖酸地平线分数通常被使用预言与急性冠的症候群(交流) 在病人为事件放血的在里面医院专业,但是这些的同类的性质为人口限制建模 ? 简单推测到另外的本地人口。我们试图在中国 patients.MethodsWe 比较三个风险模型的表演评估了三的表演为预言主要流血事件在心肌的梗塞( TIMI )由 thrombolysis 定义的在里面医院预言分数严肃(专业和未成年者)事件,在有 non-ST-elevation 交流(NSTE交流)或圣举起的中国交流病人的一个队心肌的梗塞( STEMI )。三个风险模型的刻度和辨别被 Hosmer-Lemeshow 测试和 C 统计数值分别地评估。我们由严肃的流血率是的 Delong 非参量的 test.ResultsTIMI 比较了风险分数的预兆的精确性 1.1% 外套(1.9% 和 0.86% 为 STEMI 和 NSTE 交流,分别地) 。十字军,行动和 ACUTIY 地平线分数为主要流血显示出一个足够的差别对待的能力:在全面病人, C 统计数值分别地是 0.80, 0.77,和 0.70;在 NSTE 交流病人, C 统计数值分别地是 0.73, 0.72,和 0.64;在 STEMI 病人, C 统计数值分别地是 0.91, 0.92,和 0.75。为尖酸地平线模型的 C 统计数值是比为 TIMI 的预言的 CRUSADE 和行动分数的那些显著地低的在全面病人的严肃的流血(与十字军相比, z = 3.83, P = 0.02;与行动相比, z = 3.51, P = 0.03 ) ;在 NSTE 交流病人(与十字军相比, z = 2.37, P = 0.01;与行动相比, z = 2.11, P = 0.04 ) ,并且在 STEMI 病人(与十字军相比, z = 2.6.77, P = 0.02;与行动相比, z = 7.91, P = 0.002 ) 。当 CRUSADE 和行动模型与对方相比时,没有差别被观察,不管全面病人( z = 0.68 , P = 0.31 )并且两个都交流打字(NSTE交流, z = 0.52 , P = 0.60 ),并且 STEMI 病人( z = 0.36 , P = 0.74 )。然而,三个风险分数都在我们的学习在每风险层化过高估计绝对主要流血风险。例如,在高风险层化的十字军 20 的预言的率是 11.9% 对 5.3%.ConclusionsThe 十字军和行动分数的实际的率为在里面医院专业与尖酸地平线相比流血让更大的刻度和辨别与经历一种总线标准的交流在中国病人得分。然而,他们都为中国人口过高估计流血风险率。这些风险分数的刻度将为在中国人口的归纳是有用的。 | Ran LIU Shu-Zheng LYU Guan-Qi ZHAO Wen ZHENG Xiao WANG Xue-Dong ZHAO Sheng-Hui ZHOU Lei ZHEN Shao-Ping NIE | 2017 | Journal of Geriatric Cardiology2017，14，2： | 9 |
| 6 | Coral reef-like polyanaline nanotubes prepared by a reactive template of manganese oxide for supercapacitor electrode显示文摘由 nanopaticles 组成的珊瑚像礁石的 PANI nanotubes 被锰氧化物的一个反应模板成功地综合。结构被使用 SEM, TEM,和英尺红外描绘,并且这些 nanotubes 的 supercapacitive 行为与周期的 voltammetry (CV ) 被调查,并且 chargedischarge 分别地测试。533 F/g 的一个最大的特定的电容能在 1 mol/L 被完成有 0.2 ~ 0.8 V 的潜在的范围的水的 H2SO4 (对浸透的甘汞电极) 在为 PANI 电极的一种一半房间安装配置,为电气化学的电容器在电极材料建议它的潜在的申请。 | Ling Ren Wang Fen Ran Yong Tao Tan Lei Zhao Ling Bin Kong Long Kang | 2011 | Chinese Chemical Letters2011，22，8： | 8 |
| 7 | Effects of H pylori infection on gap-junctional intercellular communication and proliferation of gastric epithelial cells in vitro显示文摘AIM: To explore the effects of H pylori infection on gap-junctional intercellular communication (GJIC) and proliferation of gastric epithelial cells in vitro. METHODS: A human gastric epithelial cell line (SGC-7901) cultured on coverslips was exposed overnight to intact H pylori (CagA+ or CagA-strains) and sonicated extracts,respectively. GJIC between the cells was detected by fluorescence redistribution after photobleaching (FRAP) technique. Proliferation of SGC cells was determined by methylthiazolyl tetrazolium (MTT) assay. RESULTS: When compared with control in which cells were cultured with simple medium alone,both CagA+ and CagA-H pylori isolates could inhibit GJIC (CagA+: F = 57.98,P < 0.01; CagA-: F = 29.59,P < 0.01) and proliferation (CagA+: F = 42.65,P < 0.01; CagA-: F = 58.14,P < 0.01) of SGC-7901 cells. Compared with CagA-strains,CagA+ H pylori more significantly down-regulated GJIC of gastric cells (intact H pylori: t = 13.86,P < 0.01; sonicated extracts: t = 11.87,P < 0.01) and inhibited proliferation gastric cells to a lesser extent in vitro (intact H pylori: t = 3.06,P < 0.05; sonicated extracts: t = 3.94,P < 0.01). CONCLUSION: Compared with CagA-H pylori strains,CagA+ strains down-regulate GJIC of gastric epithelia cells more significantly and inhibit proliferation of gastric cells to a lesser extent in vitro . H pylori ,especially CagA+ strains,may play an important role in gastric carcinogenesis. | Ran Tao Miao-Feng Hu Jin-Tu Lou Yong-Liang Lei | 2007 | World Journal of Gastroenterology2007，13，41： | 8 |
| 8 | The role of non-coding RNAs in drug resistance of oral squamous cell carcinoma and therapeutic potential显示文摘Oral squamous cell carcinoma(OSCC),the eighth most prevalent cancer in the world,arises from the interaction of multiple factors including tobacco,alcohol consumption,and betel quid.Chemotherapeutic agents such as cisplatin,5-fluorouracil,and paclitaxel have now become the first-line options for OSCC patients.Nevertheless,most OSCC patients eventually acquire drug resistance,leading to poor prognosis.With the discovery and identification of non-coding RNAs(ncRNAs),the functions of dysregulated ncRNAs in OSCC development and drug resistance are gradually being widely recognized.The mechanisms of drug resistance of OSCC are intricate and involve drug efflux,epithelial-mesenchymal transition,DNA damage repair,and autophagy.At present,strategies to explore the reversal of drug resistance of OSCC need to be urgently developed.Nano-delivery and self-cellular drug delivery platforms are considered as effective strategies to overcome drug resistance due to their tumor targeting,controlled release,and consistent pharmacokinetic profiles.In particular,the combined application of new technologies(including CRISPR systems)opened up new horizons for the treatment of drug resistance of OSCC.Hence,this review explored emerging regulatory functions of ncRNAs in drug resistance of OSCC,elucidated multiple ncRNA-meditated mechanisms of drug resistance of OSCC,and discussed the potential value of drug delivery platforms using nanoparticles and self-cells as carriers in drug resistance of OSCC. | Xiang Meng Qiu-Yue Lou Wen-Ying Yang Yue-Rong Wang Ran Chen Lu Wang Tao Xu Lei Zhang | 2021 | Cancer Communications2021，41，10： | 8 |
| 9 | Effect of moxa smoke produced during combustion of Aiye(Folium Artemisiae Argyi) on behavioral changes in mice inhaling the smoke显示文摘OBJECTIVE: To evaluate the effects that inhalation of the combustion products of the traditional Chinese herb Aiye(Folium Artemisiae Argyi) has on the central nervous system.METHODS: Forty Kunming mice(half male) were randomly assigned(n = 10/group) to a control group(C) or one of three moxa smoke concentration groups(% opacity): low(L1; 0.4%), medium(M1; 2%), and high(H1; 15%). Mice in the latter three groups were exposed to moxa smoke in a dynamic gas exposure cabinet for 20 min per day for7 days. Mice in control group were placed in the same cabinet without any intervention. For the sleep experiments, another 50 mice were divided into five groups of 10 mice each: a saline-injected control group, L1 + pentobarbital sodium(PS)-injected group, M1+PS group, H1(15%)+PS group,and a positive control group(10 mg/kg, chlorpromazine, p.o.). The weight, general activities, locomotor activities, rotarod performance, sleep duration,and sleeping rate induced by a subthreshold dose of pentobarbital sodium were recorded in the mice,and the composition of moxa smoke was analyzed using headspace gas chromatography(GC-HS).RESULTS: A low concentration of smoke significantly decreased the frequency of locomotor activities and the time for which the mice remaining on the rotarod; however, a high smoke concentration significantly prolonged the pentobarbital-induced sleeping time and sleeping rate.CONCLUSION: The concentration-dependent relaxing effects of moxa smoke on the Central Nervous System(CNS) were confirmed. Moreover, GC-HS analysis showed that the component present in the highest concentration in moxa smoke was eucalyp-tol, an essential oil well recognized for its soothing effects on the CNS. This may therefore be accountable for the sedative effects of moxa smoke. | Yang Jia Zheng Xiaojun Jin Ran Han Li Ha Lue Li Jun Wang Lei Liu Ping Chu Zhusheng Huang Chang Chang Hongsheng Lao Lixing Zhao Baixiao | 2016 | Journal of Traditional Chinese Medicine2016，36，6： | 8 |
| 10 | A comparison of atmospheric CO_2 concentration GOSAT-based observations and model simulations显示文摘Satellite observations of atmospheric CO2 are able to truly capture the variation of global and regional CO2 concentration.The model simulations based on atmospheric transport models can also assess variations of atmospheric CO2 concentrations in a continuous space and time,which is one of approaches for qualitatively and quantitatively studying the atmospheric transport mechanism and spatio-temporal variation of atmospheric CO2 in a global scale.Satellite observations and model simulations of CO2 offer us two different approaches to understand the atmospheric CO2.However,the difference between them has not been comprehensively compared and assessed for revealing the global and regional features of atmospheric CO2.In this study,we compared and assessed the spatio-temporal variation of atmospheric CO2 using two datasets of the column-averaged dry air mole fractions of atmospheric CO2(XCO2)in a year from June 2009 to May 2010,respectively from GOSAT retrievals(V02.xx)and from Goddard Earth Observing System-Chemistry(GEOS-Chem),which is a global 3-D chemistry transport model.In addition to the global comparison,we further compared and analyzed the difference of CO2 between the China land region and the United States(US)land region from two datasets,and demonstrated the reasonability and uncertainty of satellite observations and model simulations.The results show that the XCO2 retrieved from GOSAT is globally lower than GEOS-Chem model simulation by 2 ppm on average,which is close to the validation conclusion for GOSAT by ground measures.This difference of XCO2 between the two datasets,however,changes with the different regions.In China land region,the difference is large,from 0.6 to 5.6 ppm,whereas it is 1.6 to 3.7 ppm in the global land region and 1.4 to 2.7 ppm in the US land region.The goodness of fit test between the two datasets is 0.81 in the US land region,which is higher than that in the global land region(0.67)and China land region(0.68).The analysis results further indicate that the inconsistency of CO2concentration between satellite observations and model simulations in China is larger than that in the US and the globe.This inconsistency is related to the GOSAT retrieval error of CO2 caused by the interference among input parameters of satellite retrieval algorithm,and the uncertainty of driving parameters in GEOS-Chem model. | LEI LiPing GUAN XianHua ZENG ZhaoCheng ZHANG Bing RU Fei BU Ran | 2014 | Science China Earth Sciences2014，57，6： | 6 |
| 11 | Atg5 regulates late endosome and lysosome biogenesis显示文摘Autophagy is an evolutionarily conserved lysosome-based degradation process.Atg5 plays a very important role in autophagosome formation.Here we show that Atg5 is required for biogenesis of late endosomes and lysosomes in an autophagy-independent manner.In Atg5 cells,but not in other essential autophagy genes defecting cells,recycling and retrieval of late endosomal components from hybrid organelles are impaired,causing persistent hybrid organelles and defective formation of late endosomes and lysosomes.Defective retrieval of late endosomal components from hybrid organelles resulting from impaired recruitment of a component of V1-ATPase to acidic organelles blocks the pH-dependent retrieval of late endosomal components from hybrid organelles.Lowering the intracellular pH restores late endosome/lysosome biogenesis in Atg5 cells.Our data demonstrate an unexpected role of Atg5 and shed new light on late endosome and lysosome biogenesis. | PENG JunYa ZHANG Ran CUI YiTong LIU HaoDong ZHAO XiaoXin HUANG Lei HU MingXu YUAN XiaoXi MA BenYu MA XiaoWei TAKASHI Ueno MASAAKI Komatsu LIANG XingJie YU Li | 2014 | Science China(Life Sciences)2014，57，1： | 6 |
| 12 | Phenylphthalazines as small-molecule inhibitors of urea transporter UT-B and their binding model显示文摘瞄准:脲 transporters (UT ) 是明确地搬运脲的 transmembrane 蛋白质的一个家庭。没有影响电解质平衡, UT 禁止者施加利尿的活动。这研究的目的是发现新奇 UT 禁止者并且决定抑制 mechanism.Methods:主要屏蔽脲 transporter B (UT-B ) 禁止的活动用老鼠红血球细胞溶解在许多 10 000 个多样的小分子被进行试金。在与 1-phenylamino-4-phenylphthalazin 的核心结构发现成功以后, 160 类似物的 UT-B 禁止的活动与散布试金的一盏站流动灯被检验,他们的结构活动关系(SAR ) 被分析。抑制机制是进一步的在 silico assays.Results 使用调查: phenylphthalazine 混合物 PU 1424,化学上说出5-(4-(( 4-methoxyphenyl )氨基) phthalazin-1-yl )有 IC 50的 -2-methylbenzene 硫安类药剂,显示出的有势力 UT-B 抑制活动,禁止的人和鼠标 UT-B-mediated 脲运输 0.02 和 0.69 μ 珍视; mol/L ,分别地并且在更高的集中施加了100% UT-B 抑制。复合 PU 1424 没在缺乏 UT-B 的老鼠红血球影响膜脲运输。结构活动分析表明与在在 R2 位置的 R4 和酸性硫酸基的酰胺的 methoxyl 组一起的类似物在 UT-B 上展出了最高的力量抑制活动。而且,在 silico,试金验证了 R4 和 R2 在 UT-B.Conclusion 上类似物界限放到 UT-B 有约束力的衣袋和施加的抑制活动:复合 PU1424 与在 submicromolar 范围的 IC 50 是人和老鼠 UT-B 的一个新奇禁止者。它的有约束力的地点被定位在那么 UT-B 结构的地点。 | Jian-hua RAN Min LI Weng-leong TOU Tian-luo LEI Hong ZHOU Calvin Yu-Chian CHEN Bao-xue YANG | 2016 | Acta Pharmacologica Sinica2016，37，7： | 6 |
| 13 | Determination of thermal expansion coefficients for unidirectional fiber-reinforced composites显示文摘In the present work, the coefficients of thermal expansion(CTEs) of unidirectional(UD)fiber-reinforced composites are studied. First, an attempt is made to propose a model to predict both longitudinal and transverse CTEs of UD composites by means of thermo-elastic mechanics analysis. The proposed model is supposed to be a concentric cylinder with a transversely isotropic fiber embedded in an isotropic matrix, and it is subjected to a uniform temperature change. Then a concise and explicit formula is offered for each CTE. Finally, some finite element(FE) models are created by a finite element program MSC. Patran according to different material systems and fiber volume fractions. In addition, the available experimental data and results of other analytical solutions of CTEs are presented. Comparisons are made among the results of the cylinder model,the finite element method(FEM), experiments, and other solutions, which show that the predicted CTEs by the new model are in good agreement with the experimental data. In particular, transverse CTEs generally offer better agreements than those predicted by most of other solutions. | Ran Zhiguo Yan Ying Li Jianfeng Qi Zhongxing Yang Lei | 2014 | Chinese Journal of Aeronautics2014，27，5： | 5 |
| 14 | Assessment of Flood Catastrophe Risk for Grain Production at the Provincial Scale in China Based on the BMM Method显示文摘Flood catastrophe risk assessment is imperative for the steady development of agriculture under the context of global climate change,and meanwhile,it is an urgent scientific issue need to be solved in agricultural risk assessment discipline.This paper developed the methodology of flood catastrophe risk assessment,which can be shown as the standard process of crop loss calculation,Monte Carlo simulation,the generalized extreme value distribution(GEV)fitting,and risk evaluation.Data on crop loss were collected based on hectares covered by natural disasters,hectares affected by natural disasters,and hectares destroyed by natural disasters using the standard equation.Monte Carlo simulation based on appropriate distribution was used to expand sample size to overcome the insufficiency of crop loss data.Block maxima model(BMM)approach based on the extreme value theory was for modeling the generalized extreme value distribution(GEV)of flood catastrophe loss,and then flood catastrophe risk at the provincial scale in China was calculated.The Type III Extreme distribution(Weibull)has a weighted advantage of modeling flood catastrophe risk for grain production.The impact of flood catastrophe to grain production in China was significantly serious,and high or very high risk of flood catastrophe mainly concentrates on the central and eastern regions of China.Given the scenario of suffering once-in-a-century flood disaster,for majority of the major-producing provinces,the probability of 10%reduction of grain output is more than 90%.Especially,the probabilities of more than 15%decline in grain production reach up to 99.99,99.86,99.69,and 91.60%respectively in Anhui,Jilin,Liaoning,and Heilongjiang.Flood catastrophe assessment can provide multifaceted information about flood catastrophe risk that can help to guide management of flood catastrophe. | XU Lei ZHANG Qiao ZHOU Ai-lian HUO Ran | 2013 | Journal of Integrative Agriculture2013，12，12： | 5 |
| 15 | Hepatoma cell line HepG2.2.15 demonstrates distinct biological features compared with parental HepG2显示文摘AIM:To investigate the biological features of hepatitis B virus(HBV)-transfected HepG2.2.15 cells. METHODS:The cell ultrastructure,cell cycle and apoptosis,and the abilities of proliferation and invasion of HBV-transfected HepG2.2.15 and the parent HepG2 cells were examined by electron microscopy,flow cytometry, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and trans-well assay.Oncogenicity of the two cell lines was compared via subcutaneous injection and orthotopic injection or implantation in nude mice,and the pathological analysis of tumor formation was performed.Two cytoskeletal proteins were detected by Western blotting. RESULTS:Compared with HepG2 cells,HepG2.2.15 cells showed organelle degeneration and filopodia disappearance under electron microscope.HepG2.2.15 cells proliferated and migrated slowly in vitro,and hardly formed tumor and lung metastasis in nude mice.Flow cytometry showed that the majority of HepG2.2.15 cells were arrested in G1 phase,and apoptosis was minor in both cell lines.Furthermore,the levels of cytoskeletal proteins F-actin and Ezrin were decreased in HepG2.2.15 cells. CONCLUSION:HepG2.2.15 cells demonstrated a lower proliferation and invasion ability than the HepG2 cells due to HBV transfection. | Ran Zhao Tian-Zhen Wang Dan Kong Lei Zhang Hong-Xue Meng Yang Jiang Yi-Qi Wu Zu-Xi Yu Xiao-Ming Jin | 2011 | World Journal of Gastroenterology2011，17，9： | 5 |
| 16 | Anatomical characteristics of patients with symptomatic severe aortic stenosis in China显示文摘With accumulating evidence of transcatheter aortic valve replacement(TAVR)worldwide,it is gradually realized that patients being treated are different across different coun-tries,including but not limited to their age,habitus,disease etiology,aortic valve morphology,and sizes of structures.[1]In China,the average age of TAVR patients is around 5 years younger than industrialized countries,[2]making Chinese patients a good predictive sample of what the industrialized countries might see in TAVR screening in the near future due to the expansion of this technique to younger patients,but anatomical features appreciated from multi-slice computed tomography(MSCT)in the Chinese patient population have not been well demonstrated. | Tian-Yuan Xiong Yi-Ming Li Yi-Jun Yao Yu-Heng Jia Kai Xu Zhen-Fei Fang Jun Jin Guo-Sheng Fu Yi-Ning Yang Lei Jiang Wei-Dong Li Yan-Qing Wu Yan-Song Guo Ran Guo Yun-Dai Chen Yi Li Yi-Bing Shao Yi Zhang Bo-Sen Yang Yi-Ke Zhang Jing-Jing He Kai-Yu Jia Sheng-Hu He Fa-Xin Ren Jian-Cheng Xiu Xing-Hua Gu Liang-Long Chen Ke Han Yuan Feng Mao Chen | 2021 | Chinese Medical Journal2021，，22： | 5 |
| 17 | Functional assembly of root-associated microbial consortia improves nutrient efficiency and yield in soybean显示文摘Root-associated microbes are critical for plant growth and nutrient acquisition. However, scant information exists on optimizing communities of beneficial root-associated microbes or the mechanisms underlying their interactions with host plants. In this report, we demonstrate that rootassociated microbes are critical influencers of host plant growth and nutrient acquisition. Three synthetic communities(SynComs) were constructed based on functional screening of 1,893 microbial strains isolated from root-associated compartments of soybean plants. Functional assemblage of SynComs promoted significant plant growth and nutrient acquisition under both N/P nutrient deficiency and sufficiency conditions.Field trials further revealed that application of SynComs stably and significantly promoted plant growth, facilitated N and P acquisition, and subsequently increased soybean yield. Among the tested communities, SynCom1 exhibited the greatest promotion effect, with yield increases of up to 36.1% observed in two field sites. Further RNA-seq implied that SynCom application systemically regulates N and P signaling networks at the transcriptional level, which leads to increased representation of important growth pathways, especially those related to auxin responses. Overall,this study details a promising strategy for constructing SynComs based on functional screening,which are capable of enhancing nutrient acquisition and crop yield through the activities of beneficial root-associated microbes. | Cunhu Wang Yanjun Li Mingjia Li Kefei Zhang Wenjing Ma Lei Zheng Hanyu Xu Baofeng Cui Ran Liu Yongqing Yang Yongjia Zhong Hong Liao | 2021 | Journal of Integrative Plant Biology2021，63，6： | 5 |
| 18 | Serine metabolism orchestrates macrophage polarization by regulating the IGF1–p38 axis显示文摘Serine metabolism is reportedly involved in immune cell functions, but whether and how serine metabolism regulates macrophage polarization remain largely unknown. Here, we show that suppressing serine metabolism, either by inhibiting the activity of the key enzyme phosphoglycerate dehydrogenase in the serine biosynthesis pathway or by exogenous serine and glycine restriction, robustly enhances the polarization of interferon-γ-activated macrophages (M(IFN-γ)) but suppresses that of interleukin-4-activated macrophages (M(IL-4)) both in vitro and in vivo. Mechanistically, serine metabolism deficiency increases the expression of IGF1 by reducing the promoter abundance of S-adenosyl methionine-dependent histone H3 lysine 27 trimethylation. IGF1 then activates the p38-dependent JAK–STAT1 axis to promote M(IFN-γ) polarization and suppress STAT6-mediated M(IL-4) activation. This study reveals a new mechanism by which serine metabolism orchestrates macrophage polarization and suggests the manipulation of serine metabolism as a therapeutic strategy for macrophage-mediated immune diseases. | Xiao Shan Penghui Hu Lina Ni Long Shen Yanan Zhang Zemin Ji Yan Cui Meihua Guo Haoan Wang Liyuan Ran Kun Yang Ting Wang Lei Wang Bin Chen Zhi Yao Vingjie Wu Qiujing Yu | 2022 | Cellular & Molecular Immunology2022，19，11： | 5 |
| 19 | The role of ezrin-associated protein network in human sperm capacitation显示文摘在精子房间的膜修正在精子 capacitation 代表关键步;然而,这些修正的分子的基础充分没被理解。Ezrin 是 ezrin/radixin/merlin 家庭的学习最好的成员。作为在外皮的细胞骨架和血浆膜蛋白质之间的 cross-linker, ezrin 贡献膜表面结构改变。而且,激活 ezrin 和 Rho 分离禁止者, RhoGDI,通过 Rho 激活支持外皮的 cytoskeleton-polymerized 肌动朊的形成。因此, ezrin,肌动朊, RhoGDI, Rho 和血浆膜蛋白质在 vivo 形成一个复杂网络,它贡献膜表面的结构的集会。以前,我们证明 ezrin 和 RhoGDI1 在人的睾丸被表示。因此,我们寻求了决定 ezrin 鈥揜h oGDI1 鈥揳c 听鈥搈e mbrane 蛋白质网络是否在人的精子 capacitation 有一个角色。我们由西方的污点的结果显示 ezrin 被 threonine567 残余的 phosphorylation 在 capacitation 期间激活。Co-immunoprecipitation 研究表明在精子 capacitation 期间,在 ezrin 和 RhoGDI1 之间的相互作用增加,并且二维的电气泳动胶化的 phosphostaining 证明 RhoGDI1 是 phosphorylated,建议 RhoGDI1 从 RhoA 分裂并且导致精子头上的肌动朊聚合。我们推测那激活的 ezrin 在 capacitation 以后与 polymerized 肌动朊和 glycosylated 膜蛋白质 cd44 交往。用 ezrin 特定或肌动朊特定的 monoclonal 抗体堵住精子 capacitation 减少他们的 acrosome 反应(AR ) 率,但是没独自在 AR 上有效果。一起拿,我们的结果证明由 ezrin, RhoGDI1, RhoA, F 肌动朊和膜蛋白质组成的一个网络工作影响在人的精子 capacitation 期间发生在精子头的膜上的修正。 | Lei Wang Wen Chen Chun Zhao Ran Huo Xue-Jiang Guo Min Lin Xiao-Yan Huang Yun-Dong Mao Zuo-Min Zhou Jia-Hao Sha | 2010 | Asian Journal of Andrology2010，12，5： | 4 |
| 20 | Milk production and composition and metabolic alterations in the mammary gland of heat-stressed lactating dairy cows显示文摘This experiment was conducted to investigate the effects of heat stress(HS) on the feed intake, milk production and composition and metabolic alterations in the mammary gland of dairy cows. Twenty Holstein cows were randomly assigned to one of two treatments according to a completely randomized design. Half of the cows were allocated to the HS group in August(summer season), and the other half were assigned to the HS-free group in November(autumn season). HS reduced(P<0.01) dry matter intake(DMI), milk yield, milk protein and milk urea nitrogen(MUN) of cows compared with HSfree control, but increased(P<0.01) milk somatic cell counts(SCC). We determined the HS-induced metabolic alterations and the relevant mechanisms in dairy cows using liquid chromatography mass spectrometry combined with multivariate analyses. Thirty-four metabolites were identified as potential biomarkers for the diagnosis of HS in dairy cows. Ten of these metabolites, glucose, lactate, pyruvate, lactose, β-hydroxybutyrate, citric acid, α-ketoglutarate, urea, creatine, and orotic acid, had high sensitivity and specificity for HS diagnoses, and seven metabolites were also identified as potential biomarkers of HS in plasma, milk, and liver. These substances are involved in glycolysis, lactose, ketone, tricarboxylic acid(TCA), amino acid and nucleotide metabolism, indicating that HS mainly affects lactose, energy and nucleotide metabolism in the mammary gland of lactating dairy cows. This study suggested that HS might affect milk production and composition by affecting the feed intake and substance metabolisms in the mammary gland tissue of lactating dairy cows. | FAN Cai-yun SU Di TIAN He HU Rui-ting RAN Lei YANG Ying SU Yan-jing CHENG Jian-bo | 2019 | Journal of Integrative Agriculture2019，18，12： | 4 |
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