|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | The Epitope Study on the SARS-CoV Nucleocapsid Protein显示文摘The nucleocapsid protein (N protein) has been found to be an antigenic protein in a number of coronaviruses. Whether the N protein in severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is antigenic remains to be elucidated. Using Western blot and Enzyme-linked Immunosorbent Assay (ELISA),the recombinant N proteins and the synthesized peptides derived from the N protein were screened in sera from SARS patients. All patient sera in this study displayed strong positive immunoreactivities against the recombinant N proteins,whereas normal sera gave negative immunoresponses to these proteins, indicating that the N protein of SARS-CoV is an antigenic protein. Furthermore, the epitope sites in the N protein were determined by competition experiments, in which the recombinant proteins or the synthesized peptides competed against the SARS-CoV proteins to bind to the antibodies raised in SARS sera. One epitope site located at the C-terminus was confirmed as the most antigenic region in this protein. A detailed screening of peptide with ELISA demonstrated that the amino sequence from Codons 371 to 407 was the epitope site at the C-terminus of the N protein. Understanding of the epitope sites could be very significant for developing an effective diagnostic approach to SARS. | Shuting Li, Liang Lin, Hao Wang, Jianning Yin, Yan Ren, Zhe Zhao, Jie Wen, Cuiqi Zhou, Xumin Zhang, Xiaolei Li, Jingqiang Wang, Zhengfeng Zhou, Jinxiu Liu, Jianmin Shao, Tingting Lei, Jianqiu Fang, Ningzhi Xu, and Siqi LiuBeijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China & Beijing Proteomics Institute, Beijing 101300, China | 2003 | Genomics, Proteomics & Bioinformatics2003，1，3： | 9 |
| 2 | A study of micropool lubricated cutting tool in machining of mild steel显示文摘 | Shuting Lei Sasikumar Devarajan Zenghu Chang | 2008 | Journal of Materials Processing Tech2008，，3： | 1 |
| 3 | Fuzzy adaptive networks in machining process modeling:surface roughness prediction for turning operations显示文摘 | Jiao Yue Lei Shuting Z J Pei E S Lee | 2004 | International Journal of Machine Tools and Manufacture2004，44，15： | 1 |
| 4 | Distinct element modeling of laser assisted machining of silicon nitride ceramics 显示文摘 | Shuting Lei | 2003 | Computer methods in applied mechanics and engineering2003，，112： | 1 |
| 5 | A study of micropool lubricated cutting tool in machining of mild steel 显示文摘 | Shuting Lei Sasikumar Devarajan Zenghu Chang | 2009 | Journal of Materials Processing Technology2009，209，： | 1 |
| 6 | Effect of two typical focus positions on microstructure shape and morphology in femtosecond laser multi-pulse ablation of metals显示文摘 | Wenjun Wang Xuesong Mei Gedong Jiang Shuting Lei Chengjuan Yang | 2008 | Applied Surface Science2008，，： | 1 |
| 7 | A study of micropool lubricated cutting tool in ma- chining of mild steel显示文摘 | Shuting Lei Sasikumar Devarajan Zenghu Chang | 2009 | Joumal of Materials Pro- cessing Technology2009，，209： | 1 |
| 8 | Ultrasensitive assays for detection of plasma tau and phosphorylated tau 181 in Alzheimer's disease:a systematic review and meta-analysis显示文摘A lack of convenient and reliable biomarkers for diagnosis and prognosis is a common challenge for neurodegenerative diseases such as Alzheimer's disease(AD).Recent advancement in ultrasensitive protein assays has allowed the quantification of tau and phosphorylated tau proteins in peripheral plasma.Here we identified 66 eligible studies reporting quantification of plasma tau and phosphorylated tau 181(ptau181)using four ultrasensitive methods.Meta-analysis of these studies confirmed that the AD patients had significantly higher plasma tau and ptau181 levels compared with controls,and that the plasma tau and ptau181 could predict AD with high-accuracy area under curve of the Receiver Operating Characteristic Therefore,plasma tau and plasma ptau181 can be considered as biomarkers for AD diagnosis. | Xulong Ding Shuting Zhang Lijun Jiang Lu Wang Tao Li Peng Lei | 2021 | Translational Neurodegeneration2021，10，1： | 1 |
| 9 | A study of mieropool lubricated curing tool in machining of mild steel显示文摘 | Lei Shuting Devarajan S Chang Zenghu | 2009 | Journal of Materials Processing Technology2009，209，： | 1 |
| 10 | High Speed Machining of Titanium Alloys Using the Driven Rotary Tool 显示文摘 | Shuting Lei Wenjie Liu | 2002 | International Journal of Machine Tools & Manufacture2002，42，： | 1 |
| 11 | Distinct Element Modeling of Laser Assisted Machining of Silicon Nitride Ceramics显示文摘 | Lei Shuting | 2003 | Computer Methods in Applied Mechanics and Engi- neering2003，112，： | 1 |
| 12 | A study of micropool lubricated cutting tool in machining of mild steel显示文摘 | Lei Shuting Devarajan S Chang Zenghu | 2009 | Journal of Materials Processing Technology2009，209，2009： | 1 |
| 13 | Experimentalstudy of rock cutting under external hydrostaticpressure显示文摘 | PRASAD KAITKAY SHUTING LEI | 2005 | Journal of Materials ProcessingTechnology2005，159，2： | 1 |
| 14 | Distinct element modeling of laser assisted machining of silicon nitride ceramics显示文摘 | Shuting Lei | 2003 | Computer methods in applied mechanics and engineering2003，112，： | 1 |
| 15 | A study of micropool lubricated cutting tool in machining of mild steel 显示文摘 | Shuting Lei Sasikumar Devarajan Zenghu Chang | 2009 | Journal of Materials Processing Technology2009，209，3： | 1 |
| 16 | A study of micropool luhricated cutting tool in machining of mild steel显示文摘 | Shuting Lei Sasikumar Devarajan Zenghu Changc | 2009 | Journal of Materials Processing Technology2009，209，3： | 1 |
| 17 | A study of micro-pool lubricated cutting tool in machining of mild steel 显示文摘 | Lei Shuting Devarajan S Chang Zenghu | 2009 | Journal of Materials Processing Technology2009，209，3： | 1 |
| 18 | Evaluation of antimicrobial and anticancer activities of three peptides identified from the skin secretion of Hylarana latouchii显示文摘The skins of frogs of the family Ranidae are particularly rich sources of biologically active peptides, among which antimicrobial peptides (AMPs) constitute the major portion. Some of these have attracted the interest of researchers because they possess both antimicrobial and anticancer activities. In this study, with ‘shotgun’ cloning & MS/MS fragmentation, three AMPs, homologues of family brevinin-1 (brevinin-1HL), & temporin (temporin-HLa and temporin-HLb), were discovered from the skin secretion of the broad-folded frog, Hylarana latouchii. They exhibited various degrees of antimicrobial & antibiofilm activities against test microorganisms & hemolysis on horse erythrocytes. It was found that they could induce bacteria death through disrupting cell membranes & binding to bacterial DNA. In addition, they also showed different potencies towards human cancer cell lines. The secondary structure & physicochemical properties of each peptide were investigated to preliminarily reveal their structure–activity relationships. Circular dichroism spectrometry showed that they all adopted a canonical α-helical conformation in membrane-mimetic solvents. Notably, the prepropeptide of brevinin-1HL from H. latouchii was highly identical to that of brevinin-1GHd from Hylarana guentheri, indicating a close relationship between these two species. Accordingly, this study provides candidates for the design of novel anti-infective & antineoplastic agents to fight multidrug-resistant bacteria & malignant tumors & also offers additional clues for the taxonomy of ranid frogs. | Yan Lin Tianxing Lin Ningna Cheng Shuting Wu Jiancai Huang Xiaoling Chen Tianbao Chen Mei Zhou Lei Wang Chris Shaw | 2021 | Acta Biochimica et Biophysica Sinica2021，53，11： | 0 |
| 19 | Copper-linked IT MoS2/Cu2O Heterostructure for Efficient Photocatalytic Hydrogen Evolution显示文摘Metallic IT phase molybdenum disulfide(lT MoS2)is an excellent catalyst due to the abundant active sites and good conductivity.However,complex synthesis and unstable nature of IT MoS2 still limit its practical application.Herein,we propose a simple method to trigger the phase transition of MoS2.This phase transition is first predicted by density functional theory(DFT)calculations and then confirmed by Raman spectroscopy and high-resolution transmission electron microscopy(HRTEM).IT MoS2/CU2O heterostructure catalyst is then developed,showing enhanced photocatalytic hydrogen evolution. | YIN Yage WEI Shuting ZHANG Lei GUO Ziwang HUANG Haihua SAl Shiran WU Jiandong XU Yanchao LIU Ying ZHENG Lirong FAN Xiaofeng CUI Xiaogiang | 2020 | Chemical Research in Chinese Universities2020，36，6： | 0 |
| 20 | Synthesis and electrochemical properties of Th-doped LiMn_2O_4 powders for lithium-ion batteries显示文摘To improve the cycle performance of eco-friendly and cost-effective spinel LiMn2O4 as the Li secondary batteries, the Th-doped LiThxMn1-xO4 spinel powers were synthesized by solid-state method. The starting materials, Li2CO3, MnO2 and Th(NO3)4·4H2O, were mixed uniformly using a traditional ball milling, which resulted in a uniform particle size distribution in the mixed powers. Tests of X-ray diffraction, SEM, impedance spectra and charge-discharge were carried out for LiThxMn1-xO4 cathode materials. Results show that the synthesized LiTh0.01Mn1.99O4 material exhibits standard spinel structure, regular particle morphology and excellent property of charge-discharge for big current. The capacity retention of the material modified by doping Th is more than 85.1% of the first discharge specific capacity of 111.5 mAh·g-1 after 20 cycles at the current rate 1C, while the pristine LiMn2O4 is only 57% of the first discharge specific capacity of 110.2 mAh·g-1 after the same cycles at the same current rate. | LI Chao FAN Yanliang LI Shuzhong XIE Bing BI Lei YANG Shuting | 2006 | Rare Metals2006，25，z1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员