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| 1 | Non-invasive assessment of barrier integrity and function of the human gut显示文摘Over the past decades evidence has been accumulating that intestinal barrier integrity loss plays a key role in the development and perpetuation of a variety of disease states including inflammatory bowel disease and celiac disease,and is a key player in the onset of sepsis and multiple organ failure in situations of intestinal hypoperfusion,including trauma and major surgery.Insight into gut barrier integrity and function loss is important to improve our knowledge on disease etiology and pathophysiology and contributes to early detection and/or secondary prevention of disease.A variety of tests have been developed to assess intestinal epithelial cell damage,intestinal tight junction status and consequences of intestinal barrier integrity loss,i.e.increased intestinal permeability.This review discusses currently available methods for evaluating loss of human intestinal barrier integrity and function. | Joep Grootjans Geertje Thuijls Froukje Verdam Joep PM Derikx Kaatje Lenaerts Wim A Buurman | 2010 | World Journal of Gastrointestinal Surgery2010，2，3： | 18 |
| 2 | Life and death at the mucosal-luminal interface: New perspectives on human intestinal ischemia-reperfusion显示文摘Intestinal ischemia is a frequently observed phenomenon. Morbidity and mortality rates are extraordinarily high and did not improve over the past decades. This is in part attributable to limited knowledge on the pathophysiology of intestinal ischemia-reperfusion(IR) in man, the paucity in preventive and/or therapeutic options and the lack of early diagnostic markers for intestinal ischemia. To improve our knowledge and solve clinically important questions regarding intestinal IR, we developed a human experimental intestinal IR model. With this model, we were able to gain insight into the mechanisms that allow the human gut to withstand short periods of IR without the development of severe inflammatory responses. The purpose of this review is to overview the most relevant recent advances in our understanding of the pathophysiology of human intestinal IR, as well as the(potential) future clinical implications. | Joep Grootjans Kaatje Lenaerts Wim A Buurman Cornelis HC Dejong Joep PM Derikx | 2016 | World Journal of Gastroenterology2016，22，9： | 11 |
| 3 | High prevalence of epilepsy in onchocerciasis endemic health areas in Democratic Republic of the Congo显示文摘Background:A high prevalence of epilepsy has been observed in many onchocerciasis endemic regions.This study is to estimate the prevalence of active epilepsy and exposure to Onchocerca volvulus infection in a rural population in Ituri province,Democratic Republic of Congo.Methods:In August 2016,a community-based cross-sectional study was conducted in an onchocerciasis endemic area in the rural health zone of Logo,Ituri Province.Households within two neighbouring health areas were randomly sampled.To identify persons with epilepsy,a three-stage approach was used.In the first stage,all individuals of the selected households were screened for epilepsy by non-medical field workers using a validated 5-item questionnaire.In the second and third stage,suspected cases of epilepsy were examined by non-specialist medical doctors,and by a neurologist,respectively.A case of epilepsy was defined according to the 2014 International League Against Epilepsy(ILAE)guidelines.Exposure to O.volvulus was assessed by testing for IgG4 antibodies to an O.volvulus antigen(OV16 Rapid Test,)in individuals aged 3 years and older.Results:Out of 1389 participants included in the survey,64 were considered to have active epilepsy(prevalence 4.6%)(95%confidence interval[CI]:3.6-5.8).The highest age-specific epilepsy prevalence estimate was observed in those aged 20 to 29 years(8.2%).Median age of epilepsy onset was 10 years,with a peak incidence of epilepsy in the 10 to 15 year-old age group.OV16 test results were available for 912 participants,of whom 30.5%(95%CI,27.6-33.6)tested positive.The prevalence of OV16 positivity in a village ranged from 8.6 to 68.0%.After adjusting for age,gender and ivermectin use,a significant association between exposure to onchocerciasis and epilepsy was observed(adjusted odds ratio=3.19,95%CI:1.63-5.64)(P<0.001).Conclusions:A high prevalence of epilepsy and a significant association between epilepsy and exposure to O.volvulus were observed in the population in Ituri province,Democratic Republic of Congo.There is an urgent need to implement a CDTI programme and to scale up an epilepsy treatment and care programme. | Evy Lenaerts Michel Mandro Deby Mukendi Patrick Suykerbuyk Housseini Dolo Deogratias Wonya’Rossi Françoise Ngave Chellafe Ensoy-Musoro Anne Laudisoit An Hotterbeekx Robert Colebunders | 2018 | Infectious Diseases of Poverty2018，7，1： | 5 |
| 4 | Human small intestine is capable of restoring barrier function after short ischemic periods显示文摘AIM To assess intestinal barrier function during human intestinal ischemia and reperfusion(IR).METHODS In a human experimental model,6 cm of jejunum was selectively exposed to 30 min of ischemia(I) followed by 30 and 120 min of reperfusion(R). A sham procedure was also performed. Blood and tissue was sampled at all-time points. Functional barrier function was assessed using dual-sugar absorption tests with lactulose(L) and rhamnose(R). Plasma concentrations of citrulline,an amino acid described as marker for enterocyte function were measured as marker of metabolic enterocytes restoration. Damage to the epithelial lining was assessed by immunohistochemistry for tight junctions( TJs),by plasma marker for enterocytes damage(I-FABP) and analyzed by electron microscopy(EM) using lanthanum nitrate as an electrondense marker.RESULTS Plasma L/R ratio's were significantly increased after 30 min of ischemia(30 I) followed by 30 min of reperfusion(30 R) compared to control(0.75 ± 0.10 vs 0.20 ± 0.09,P < 0.05). At 120 min of reperfusion(120 R),ratio's normalized(0.17 ± 0.06) and were not significantly different from control. Plasma levels of I-FABP correlated with plasma L/R ratios measured at the same time points(correlation: 0.467,P < 0.01). TJs staining shows distortion of staining at 30 I. An intact lining of TJs was again observed at 30 I120 R. Electron microscopy analysis revealed disrupted TJs after 30 I with paracellular leakage of lanthanum nitrate,which restored after 30 I120 R. Furthermore,citrulline concentrations closely paralleled the histological perturbations during intestinal IR.CONCLUSION This study directly correlates histological data with intestinal permeability tests,revealing that the human gut has the ability of to withstand short episodes of ischemia,with morphological and functional recovery of the intestinal barrier within 120 min of reperfusion. | Dirk HSM Schellekens Inca HR Hundscheid Claire AJI Leenarts Joep Grootjans Kaatje Lenaerts Wim A Buurman Cornelis HC Dejong Joep PM Derikx | 2017 | World Journal of Gastroenterology2017，23，48： | 4 |
| 5 | Evaluation of refapentine in long-term treatment regimens for tuberulosis in mice显示文摘 | Lenaerts AM Chase SE Chmielewski A J | 1999 | Antimicro Agents Chemother1999，43，10： | 1 |
| 6 | Ultrastruetural and func- tional remodeling of the coupling between Ca2+ influx and sarco- plasmic reticulum Ca2+ release in right atrial myoeytes from ex- perimental persistent atrial fibrillation显示文摘 | Lenaerts I Bito V Heinzel FR | 2009 | Circ Res2009，105，： | 1 |
| 7 | Cerebral venous thrombosis and procoagulant factors 显示文摘 | Lefebvre P Lierneux B Lenaerts L | 1998 | Angiology1998，49，： | 1 |
| 8 | Arginine deficiency in preconfluent intestinal Caco-2 cells modulates expression of proteins involved in proliferation, apoptosis, and heat shock response 显示文摘 | Lenaerts K Renes J Bouwman FG | 2007 | Proteomics2007，7，4： | 1 |
| 9 | Porous Flow in Particle -based Fluid Simulations显示文摘 | Toon Lenaerts Bart Adams Philip Dutre | 2008 | ACM Transactions on Graphics2008，27，3： | 1 |
| 10 | Evaluation of a 2-pyridone, KRQ- 10018, against Mycobacterium tuberculosis in vitro and in vivo 显示文摘 | Anne J Lenaerts Casey B | 2008 | Antimicrob Agents Cbemother2008，52，4： | 1 |
| 11 | Application of laser ablation inductively coupled plasma (dynamic reaction cell) mass spectrometry for depth profiling analysis of high-tech industrial materi- als显示文摘 | BALCAEN LIEVE I L LENAERTS J MOENS L | 2005 | J Anal At Spectrom2005，20，： | 1 |
| 12 | Evaluation of refapentine in long term treatment regimens for tuberculosis in mice显示文摘 | Lenaerts AM Chase SE Chrnielewski AJ etal | 1999 | Antimicro Agents Chemother1999，43，： | 1 |
| 13 | VTT Benchmark: Application of the Restoring Force Surface Method显示文摘 | Kersehen G Lenaerts V GollnVal J C | 2003 | Mechanical Systems and Signal Professing2003，17，1： | 1 |
| 14 | Cerebral venous thrombosis and procoagulant factors显示文摘 | Lefbvre P Liemeux B Lenaerts L | 1998 | Angiology1998，49，7： | 1 |
| 15 | Improved fluorescent in situ hybridization method for detection of bacteria from activated sludge and river water by using DNA molecular beacons and flow cytometry 显示文摘 | Lenaerts J Lappin-Scott H M Porter J | 2007 | Applied and Environmentol Microbiology2007，73，6： | 1 |
| 16 | Antiviral therapy for adenovirus infections显示文摘 | Lenaerts L Naesens L | | 0，，： | 1 |
| 17 | Preclinical testing of nitroimidazopyran PA - 824 for activity against Mycobacterium tuberculosis in a series of in vitro and in vivo models 显示文摘 | Lenaerts AJ Cruppo V Marietta KS | 2005 | Antimicrob Agents Chemother2005，49，6： | 1 |
| 18 | Clinical features and treat- ment of adenovirus infections 显示文摘 | Lenaerts L De Clercq E Naesens L | 2008 | Rev Med Virol2008，18，6： | 1 |
| 19 | Starvation compromises Paneth ceils 显示文摘 | Hodin CM Lenaerts K Grootjans J | 2011 | Am J Pathol2011，179，： | 1 |
| 20 | Cerebral venous thrombosis and procoagulant factors显示文摘 | Lefbvre P Liemeux B Lenaerts L | 1998 | Angiology1998，49，7： | 1 |
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