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| 1 | SMALL ORGAN SIZE 1 and SMALL ORGAN SIZE 2/DWARF AND LOW-TILLERING Form aComplex to Integrate Auxin and Brassinosteroid Signaling in Rice显示文摘尽管植物生长素和 brassinosteroid (BR ) synergistically 控制各种各样的植物回答,位于 auxin-BR 串音下面的分子的机制很好没被理解。我们以前识别了 SMOS1,一个调整植物生长素的 APETALA2 类型抄写因素,作为小机关的原因的基因缩放 1 (smos1 ) 被各种各样的机关的一种减少的最后的尺寸在米饭描绘的异种。在这研究,我们识别了另一 smos 异种, smos2,哪个表演与 smos1 难区分的显型。SMOS2 与以前报导的矮子和 LOW-TILLERING (DLT ) 相同,它编码涉及 BR 发信号的 GRAS 蛋白质。SMOS1 和 SMOS2/DLT 身体上交往合作地在酵母并且在米饭原子核提高 transcriptional transactivation 活动。一致地, OsPHI-1 的表示, SMOS1 的一个直接目标,当 SMOS1 和 SMOS2/DLT 蛋白质两个都是在米饭房间的现在时,仅仅是 upregulated。一起拿,我们的结果建议 SMOS1 和 SMOS2/DLT 在在米饭表明串音的 auxin-BR 上形成重点建筑群。 | Ko Hirano Hideki Yoshida Koichiro Aya Mayuko Kawamura Makoto Hayashi Tokunori Hobo Karma Sato-lzawa Hidemi Kitano Miyako Ueguchi-Tanaka Makoto Matsuoka | 2017 | Molecular Plant2017，10，4： | 20 |
| 2 | Insulin-like growth factor-I receptor in proliferation and motility of pancreatic cancer显示文摘AIM:To develop a molecular therapy for pancreatic cancer, the insulin-like growth factor-I (IGF-I) signaling pathway was analyzed.METHODS: Pancreatic cancer cell lines (MIA-Paca2, NOR-P1, PANC-1, PK-45H, PK-1, PK-59 and KP-4) were cultured in media with 10 mL/L fetal bovine serum. Western blotting analysis was performed to clarify the expression of IGF-I receptor (IGF-IR). Picropodophyllin (PPP), a specific inhibitor of IGF-IR, LY294002, a specific inhibitor of phosphatidylinositol3 kinase (PI3K), and PD98059, a specific inhibitor of mitogen-activated protein kinase, were added to the media. After 72 h, a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt (MTS) assay was performed to analyze cell proliferation. A wound assay was performed to analyze cell motility with hematoxylin and eosin (HE) staining 48 h after addition of each inhibitor. RESULTS: All cell lines clearly expressed not only IGF-IR but also phosphorylated IGF-IR. PPP significantly suppressed proliferation of MIA-Paca2, NOR-P1, PANC-1, PK-45H, PK-1, PK-59 and KP-4 cells to 36.9% ± 2.4% (mean ± SD), 30.9% ± 5.5%, 23.8% ± 3.9%, 37.1% ± 5.3%, 10.4% ± 4.5%, 52.5% ± 4.5% and 22.6% ± 0.4%, at 2 μmol/L, respectively (P < 0.05). LY294002 significantly suppressed proliferation of MIA-Paca2, NOR-P1, PANC-1, PK-45H, PK-1, PK-59 and KP-4 cells to 44.4% ± 7.6%, 32.9% ± 8.2%, 53.9% ± 8.0%, 52.8% ± 4.0%, 32.3% ± 4.2%, 51.8% ± 4.5%, and 30.6% ± 9.4%, at 50 μmol/L, respectively (P < 0.05). PD98059 did not significantly suppress cell proliferation. PPP at 2 μmol/L suppressed motility of MIA-Paca2, NOR-P1, PANC-1, PK-45H, PK-1, PK-59 and KP-4 cells to 3.0% ± 0.2%, 0%, 0%, 2.0% ± 0.1%, 5.0% ± 0.2%, 3.0% ± 0.1%, and 5.0% ± 0.2%, respectively (P < 0.05). LY294002 at 50 μmol/L suppressed motility of MIA-Paca2, NOR-P1, PANC-1, PK-45H, PK-1, PK-59 and KP-4 to 3.0% ± 0.2%, 0%, 3.0% ± 0.2%, 0%, 0%, 0% and 3% ± 0.1%, respectively (P < 0.05). PD980509 at 20 μmol/L did not suppress motility. Cells were observed by microscopy to analyze the morphological changes induced by the inhibitors. Cells in medium treated with 2 μmol/L PPP or 50 μmol/L LY294002 had pyknotic nuclei, whereas those in medium with 20 μmol/L PD98059 did not show apoptosis.CONCLUSION: IGF-IR and PI3K are good candidates for molecular therapy of pancreatic cancer. | Minoru Tomizawa Fuminobu Shinozaki Takao Sugiyama Shigenori Yamamoto Makoto Sueishi Takanobu Yoshida | 2010 | World Journal of Gastroenterology2010，16，15： | 12 |
| 3 | Super-low friction of ta-C coating in presence of oleic acid显示文摘Friction tests have been carried out on bearing steel friction pairs coated with a-C:H and ta-C under lubricated conditions with 1-hexadecene and pure oleic acid,respectively.Main results show that ta-C leads to the super-low regime with oleic acid(friction coefficient below 0.01)and that a-C:H is quite well lubricated by 1-hexadecen.Surface analyses by coupling X-ray photoelectron spectroscopy(XPS)and secondary ion mass spectrometry(SIMS)have been carried out in the case of oleic acid with ta-C and a-C:H coatings.Results show that tribochemical reactions have occurred.OH groups terminate the ta-C surface whereas a-C:H is covered by a thin tribofilm and is terminated by oleic acid monolayers.The superlow regime in the case of ta-C is mainly attributed to the presence of the OH-termination. | Makoto KANO Jean Michel MARTIN Kentaro YOSHIDA Maria Isabel DE BARROS BOUCHET | 2014 | Friction2014，2，2： | 6 |
| 4 | Sphingosine kinase 1 is upregulated with lysophosphatidic acid receptor 2 in human colorectal cancer显示文摘AIM: To examine the expression of Sph K1, an oncogenic kinase that produces sphingosine 1-phosphate(S1P), and its correlation with the expression of LPAR2, a major lysophosphatidic acid(LPA) receptor overexpressed in various cancers, in human colorectal cancer.METHODS: Real-time reverse-transcription polymerase chain reaction was used to measure the m RNA expression of Sph K1, LPAR2, and the three major S1 P receptors in 27 colorectal cancer samples and corresponding normal tissue samples. We also examined the correlation between the expression of Sph K1 and LPAR2.RESULTS: C o l o r e c t a l c a n c e r t i s s u e i n 2 2 o f 2 7 patients had higher levels of Sph K1 m RNA than in normal tissue. In two-thirds of the samples, Sph K1 m RNA expression was more than two-fold higher than in normal tissue. Consistent with previous reports, LPAR2 m RNA expression in 20 of 27 colorectal cancer tissue samples was higher compared to normal tissue samples. Expression profiles of all three major S1 P receptors, S1PR1, S1PR2, and S1PR3, varied without any trend, with no significant difference in expression between cancer and normal tissues. A highly significant positive correlation was found between Sph K1 and LPAR2 expression [Pearson's correlation coefficient(r) = 0.784 and P < 0.01]. The m RNA levels of Sph K1 and LPAR2 did not correlate with TNM stage.CONCLUSION: Our findings suggest that S1 P andLPA may play important roles in the development ofcolorectal cancer via the upregulation of Sph K1 andLPAR2, both of which could serve as new therapeutictargets in the treatment of colorectal cancer. | Dai Shida Satoru Inoue Yuki Yoshida Atsushi Kodaka Tsutomu Tsuji Makoto Tsuiji | 2016 | World Journal of Gastroenterology2016，22，8： | 6 |
| 5 | Factors associated with residual gastroesophageal reflux disease symptoms in patients receiving proton pump inhibitor maintenance therapy显示文摘AIM To elucidate the factors associated with residual gastroesophageal reflux disease(GERD) symptoms in patients receiving proton pump inhibitor(PPI) maintenance therapy in clinical practice.METHODS The study included 39 GERD patients receiving maintenance PPI therapy. Residual symptoms were assessed using the Frequency Scale for Symptoms of GERD(FSSG) questionnaire and the Gastrointestinal Symptom Rating Scale(GSRS). The relationships between the FSSG score and patient background factors, including the CYP2C19 genotype, were analyzed.RESULTS The FSSG scores ranged from 1 to 28 points(median score: 7.5 points), and 19 patients(48.7%) had a score of 8 points or more. The patients' GSRS scores were significantly correlated with their FSSG scores(correlation coefficient = 0.47, P < 0.005). In erosive esophagitis patients, the FSSG scores of the CYP2C19 rapid metabolizers(RMs) were significantly higher than the scores of the poor metabolizers and intermediate metabolizers(total scores: 16.7 ± 8.6 vs 7.8 ± 5.4, P < 0.05; acid reflux-related symptom scores: 12 ± 1.9 vs 2.5 ± 0.8, P < 0.005). In contrast, the FSSG scores of the CYP2C19 RMs in the non-erosive reflux disease patients were significantly lower than those of the other patients(total scores: 5.5 ± 1.0 vs 11.8 ± 6.3, P < 0.05; dysmotility symptom-related scores: 1.0 ± 0.4 vs 6.0 ± 0.8, P < 0.01). CONCLUSION Approximately half of the GERD patients receiving maintenance PPI therapy had residual symptoms associated with a lower quality of life, and the CYP2C19 genotype appeared to be associated with these residual symptoms. | Fumiaki Kawara Tsuyoshi Fujita Yoshinori Morita Atsushi Uda Atsuhiro Masuda Masaya Saito Makoto Ooi Tsukasa Ishida Yasuyuki Kondo Shiei Yoshida Tatsuya Okuno Yoshihiko Yano Masaru Yoshida Hiromu Kutsumi Takanobu Hayakumo Kazuhiko Yamashita Takeshi Hirano Midori Hirai Takeshi Azuma | 2017 | World Journal of Gastroenterology2017，23，11： | 5 |
| 6 | endoscopic ultrasonography-guided biliary drainage:Who,when,which,and how?显示文摘Both endoscopic ultrasonography(EUS)-guided choledochoduodenostomy( EUS- CDS) and EUS-guided hepaticogastrostomy(EUS-HGS) are relatively well established as alternatives to percutaneous transhepatic biliary drainage(PTBD). Both EUSCDS and EUS-HGS have high technical and clinical success rates(more than 90%) in high-volume centers. Complications for both procedures remain high at 10%-30%. Procedures performed by endoscopists who have done fewer than 20 cases sometimes result in severe or fatal complications. When learning EUSguided biliary drainage(EUS-BD), we recommend a mentor's supervision during at least the first 20 cases. For inoperable malignant lower biliary obstruction, a skillful endoscopist should perform EUS-BD before EUS-guided rendezvous technique(EUS-RV) and PTBD. We should be select EUS-BD for patients having altered anatomy from malignant tumors before balloon-enteroscope-assisted endoscopic retrograde cholangiopancreatography, EUS-RV, and PTBD. If both EUS-CDS and EUS-HGS are available, we should select EUS-CDS, according to published data. EUSBD will potentially become a first-line biliary drainage procedure in the near future. | Kazuo Hara Kenji Yamao Nobumasa Mizuno Susumu Hijioka Hiroshi Imaoka Masahiro Tajika Tutomu Tanaka Makoto Ishihara Nozomi Okuno Nobuhiro Hieda Tukasa Yoshida Yasumasa Niwa | 2016 | World Journal of Gastroenterology2016，22，3： | 4 |
| 7 | OsIDD2, a zinc finger and INDETERMINATE DOMAIN protein, regulates secondary cell wall formation显示文摘Previously, we found 123 transcription factors(TFs) as candidate regulators of secondary cell wall(SCW)formation in rice by using phylogenetic and co-expression network analyses. Among them, we examined in this work the role of OsIDD_2, a zinc finger and indeterminate domain(IDD) family TF. Its overexpressors showed dwarfism, fragile leaves, and decreased lignin content, which are typical phenotypes of plants defective in SCW formation, whereas its knockout plants showed slightly increased lignin content.The RNA-seq and quantitative reverse transcription polymerase chain reaction analyses confirmed that some lignin biosynthetic genes were downregulated in the OsIDD_2-overexpressing plants, and revealed the same case for other genes involved in cellulose synthesis and sucrose metabolism. The transient expression assay using rice protoplasts revealed that OsIDD_2 negatively regulates the transcription of genes involved in lignin biosynthesis, cinnamyl alcohol dehydrogenase 2 and 3(CAD_2 and 3), and sucrose metabolism, sucrose synthase 5(SUS_5), whereas an Alpha Screen assay, which can detect the interaction between TFs and their target DNA sequences, directly confirmed the interaction between OsIDD_2 and the target sequences located in the promoter regions of CAD_2 and CAD_3. Based on these observations, we conclude that OsIDD_2 is negatively involved in SCW formation and other biological events by downregulating its target genes. | Peng Huang Hideki Yoshida Kenji Yano Shunsuke Kinoshita Kyosuke Kawai Eriko Koketsu Masako Hattori Sayaka Takehara Ji Huang Ko Hirano Reynante Lacsamana Ordonio Makoto Matsuoka Miyako Ueguchi-Tanaka | 2018 | Journal of Integrative Plant Biology2018，60，2： | 3 |
| 8 | Comparison of unenhanced magnetic resonance imaging and ultrasound in detecting very small hepatocellular carcinoma显示文摘BACKGROUND In hepatocellular carcinoma(HCC),detection and treatment prior to growth beyond 2 cm are important as a larger tumor size is more frequently associated with microvascular invasion and/or satellites.In the surveillance of very small HCC nodules(≤2 cm in maximum diameter,Barcelona clinical stage 0),we demonstrated that the tumor markers alpha-fetoprotein and PIVKA-Ⅱare not so useful.Therefore,we must survey with imaging modalities.The superiority of magnetic resonance imaging(MRI)over ultrasound(US)to detect HCC was confirmed in many studies.Although enhanced MRI is now performed to accurately diagnose HCC,in conventional clinical practice for HCC surveillance in liver diseases,unenhanced MRI is widely performed throughout the world.While,MRI has made marked improvements in recent years.AIM To make a comparison of unenhanced MRI and US in detecting very small HCC that was examined in the last ten years in patients in whom MRI and US examinations were performed nearly simultaneously.METHODS In 394 patients with very small HCC nodules,those who underwent MRI and US at nearly the same time(on the same day whenever possible or at least within 14 days of one another)at the first diagnosis of HCC were selected.The detection rate of HCC with unenhanced MRI was investigated and compared with that of unenhanced US.RESULTS The sensitivity of unenhanced MRI for detecting very small HCC was 95.1%(97/102,95%confidence interval:90.9-99.3)and that of unenhanced US was 69.6%(71/102,95%confidence interval:60.7-78.5).The sensitivity of unenhanced MRI for detecting very small HCC was significantly higher than that of unenhanced US(P<0.001).Regarding the location of HCC in the liver in patients in whom detection by US was unsuccessful,S7-8 was identified in 51.7%.CONCLUSION Currently,unenhanced MRI is a very useful tool for the surveillance of very small HCC in conventional clinical follow-up practice. | Kazuo Tarao Akito Nozaki Hirokazu Komatsu Tatsuji Komatsu Masataka Taguri Katsuaki Tanaka Testuo Yoshida Hideki Koyasu Makoto Chuma Kazushi Numata Shin Maeda | 2021 | World Journal of Hepatology2021，13，6： | 3 |
| 9 | Rectosigmoid findings are not associated with proximal colon cancer: Analysis of 6 196 consecutive cases undergoing total colonoscopy显示文摘AIM: To review the risk of proximal colon cancer in patients undergoing colonoscopy.METHODS: We estimated the risk of advanced proximal adenomas and cancers in 6 196 consecutive patients that underwent colonoscopy (mean age 60 years, 65% males,without prior history of colorectal examination). Neoplasms were classified as diminutive adenoma (5 mm or less),small adenoma (6-9 mm), advanced adenoma (10 mm or more, with villous component or high-grade dysplasia)and cancer (invasive adenocarcinoma). The sites of neoplasms were defined as rectosigmoid (rectum and sigmoid colon) and proximal colon (from cecum to descending colon).RESULTS: The trend of the prevalence of advanced proximal adenoma was to increase with severe rectosigmoid findings, while the prevalence of proximal colon cancer did not increase with severe rectosigmoid findings. Among the 157 patients with proximal colon cancer, 74% had no neoplasm in the rectosigmoid colon. Multivariate logisticregression analysis revealed that age was the main predictor of proximal colon cancer and existence of rectosigmoid adenoma was not a predictor of proximal colon cancer.CONCLUSION: Sigmoidoscopy is inadequate for colorectal cancer screening, especially in older populations. | Makoto Okamoto Takao Kawabe Yutaka Yamaji Jun Kato Tsuneo Ikenoue Goichi Togo Haruhiko Yoshida Yasushi Shiratori Masao Omata | 2005 | World Journal of Gastroenterology2005，11，15： | 3 |
| 10 | Phenol-based endoscopic ultrasound-guided celiac plexus neurolysis for East Asian alcohol-intolerant upper gastrointestinal cancer patients:A pilot study显示文摘AIM:To investigate the effectiveness of phenol for the relief of cancer pain by endoscopic ultrasound-guided celiac plexus neurolysis(EUS-CPN).METHODS:Twenty-two patients referred to our hospital with cancer pain from August 2009 to July 2011for EUS-CPN were enrolled in this study.Phenol was used for 6 patients with alcohol intolerance and ethanol was used for 16 patients without alcohol intolerance.The primary endpoint was the positive response rate(pain score decreased to≤3)on postoperative day 7.Secondary endpoints included the time to onset of pain relief,duration of pain relief,and complication rates.RESULTS:There was no significant difference in the positive response rate on day 7.The rates were 83%and 69%in the phenol and ethanol groups,respectively.Regarding the time to onset of pain relief,in the phenol group,the median pre-treatment pain score was 5,whereas the post-treatment scores decreased to 1.5,1.5,and 1.5 at 2,8,and 24 h,respectively(P<0.05).In the ethanol group,the median pre-treatment pain score was 5.5,whereas the post-treatment scores significantly decreased to 2.5,2.5,and 2.5 at 2,8,and24 h,respectively(P<0.01).There was no significant difference in the duration of pain relief between the phenol and ethanol groups.No significant difference was found in the rate of complications between the 2groups;however,burning pain and inebriation occurred only in the ethanol group.CONCLUSION:Phenol had similar pain-relieving effects to ethanol in EUS-CPN.Comparing the incidences of inebriation and burning pain,phenol may be superior to ethanol in EUS-CPN procedures. | Hirotoshi Ishiwatari Tsuyoshi Hayashi Makoto Yoshida Michihiro Ono Hiroyuki Masuko Tsutomu Sato Koji Miyanishi Yasushi Sato Rishu Takimoto Masayoshi Kobune Atsushi Miyamoto Tomoko Sonoda Junji Kato | 2014 | World Journal of Gastroenterology2014，20，30： | 2 |
| 11 | Spatiotemporal changes of coxsackievirus and adenovirus receptor in rat hearts during postnatal development and in cultured cardiomyocytes of neonatal rat显示文摘 | Takeshi Kashimura Makoto Kodama Yuko Hotta Junichi Hosoya Kaori Yoshida Takuya Ozawa Ritsuo Watanabe Yuji Okura Kiminori Kato Haruo Hanawa Ryozo Kuwano Yoshifusa Aizawa | 2004 | Virchows Archiv2004，，3： | 2 |
| 12 | JPN Guidelines for the management of acute pancreatitis: epidemiology, etiology, natural history, and outcome predictors in acute pancreatitis显示文摘 | Miho Sekimoto Tadahiro Takada Yoshifumi Kawarada Koichi Hirata Toshihiko Mayumi Masahiro Yoshida Masahiko Hirota Yasutoshi Kimura Kazunori Takeda Shuji Isaji Masaru Koizumi Makoto Otsuki Seiki Matsuno | 2006 | Journal of Hepato-Biliary-Pancreatic Surgery2006，，： | 2 |
| 13 | JPN Guidelines for the management of acute pancreatitis: epidemiology, etiology, natural history, and outcome predictors in acute pancreatitis显示文摘 | Miho Sekimoto Tadahiro Takada Yoshifumi Kawarada Koichi Hirata Toshihiko Mayumi Masahiro Yoshida Masahiko Hirota Yasutoshi Kimura Kazunori Takeda Shuji Isaji Masaru Koizumi Makoto Otsuki Seiki Matsuno | 2006 | Journal of Hepato - Biliary - Pancreatic Surgery2006，，1： | 2 |
| 14 | Phase I/II study of S-1 plus cisplatin combined with peptide vaccinesfor human vascular endothelial growth factor receptor 1 and 2 in patients withadvanced gastric cancer显示文摘 | Toru Masuzawa Yoshiyuki Fujiwara Kaoru Okada Ayumu Nakamura Shuji Takiguchi Kiyokazu Nakajima Hiroshi Miyata Makoto Yamasaki Yukinori Kurokawa Ryuji Osawa Kazuyoshi Takeda Koji Yoshida Takuya Tsunoda Yusuke Nakamura Masaki Mori Yuichiro Doki | 2012 | International Journal of Oncology2012，，4： | 2 |
| 15 | Rebamipide, a Gastroprotective Drug, Inhibits Indomethacin-Induced Apoptosis in Cultured Rat Gastric Mucosal Cells: Association with the Inhibition of Growth Arrest and DNA Damage-Induced 45α Expression显示文摘 | Yuji Naito Hirokazu Kajikawa Katsura Mizushima Makoto Shimozawa Masaaki Kuroda Kazuhiro Katada Tomohisa Takagi Osamu Handa Satoshi Kokura Hiroshi Ichikawa Norimasa Yoshida Hirofumi Matsui Toshikazu Yoshikawa | 2005 | Digestive Diseases and Sciences2005，，1： | 2 |
| 16 | Assessment of the Risk Factors for Colonic Diverticular Hemorrhage显示文摘 | Atsuo Yamada Takafumi Sugimoto Shintaro Kondo Miki Ohta Hirotsugu Watabe Shin Maeda Goichi Togo Yutaka Yamaji Keiji Ogura Makoto Okamoto Haruhiko Yoshida Takao Kawabe Tateo Kawase Masao Omata | 2008 | Diseases of the Colon & Rectum2008，，1： | 2 |
| 17 | Intraoperative ultrasonographic localization of pulmonary ground-glass opacities显示文摘 | Ryoichi Kondo Kazuo Yoshida Kazutoshi Hamanaka Masahiro Hashizume Toshiki Ushiyama Akira Hyogotani Makoto Kurai Satoshi Kawakami Mana Fukushima Jun Amano | 2009 | The Journal of Thoracic and Cardiovascular Surgery2009，，4： | 2 |
| 18 | Markers of insulin resistance in day and shift workers aged 30–59 years显示文摘 | Teruo Nagaya Hideyo Yoshida Hidekatsu Takahashi Makoto Kawai | 2002 | International Archives of Occupational and Environmental Health2002，，8： | 1 |
| 19 | Effect of dilution rate on competitive interactions between the cyanobacterium Microcystis novacekii and the green alga scenedesmus quadricauda in mixed chemostat cultures显示文摘 | KAORU TAKEYA AKIRA KUWATA MAKOTO YOSHIDA | 2004 | Journal of Plankton Research2004，26，1： | 1 |
| 20 | Role of endobronchial ultrasound-guided transbronchial needle aspiration in the management of lung cancer显示文摘 | Kazuhiro Yasufuku Takahiro Nakajima Taiki Fujiwara Masako Chiyo Akira Iyoda Shigetoshi Yoshida Makoto Suzuki Yasuo Sekine Kiyoshi Shibuya Ichiro Yoshino | 2008 | General Thoracic and Cardiovascular Surgery2008，，6： | 1 |
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