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| 1 | Hepatitis C virus infection down-regulates the expression of peroxisome proliferator-activated receptor a and carnitine palmitoyl acyl-CoA transferase 1A显示文摘AIM: To elucidate the role of the peroxisome proliferator-activated receptor α (PPARα) and its target gene carnitine palmitoyl acyl-CoA transferase 1A (CPT1A)in the pathogenesis of hepatitis C virus (HCV) infection.METHODS: Liver samples were collected from the patients with chronic HCV infection and controls. HepG2cells were transfected with vector pEF352neo carrying.Two independent clones (clone N3 and N4) stably expressing HCV core protein were analyzed. Total RNA was extracted from cells and liver tissues. PPARα and CPT1A mRNAs were quantified by real-time polymerase chain reaction (PCR) using SYBR Green Master. Total extracted proteins were separated by polyacrylamide gel electrophoresis, and electroblotted. Membranes were incubated with the anti-PPARα antibody, then with a swine anti-rabbit IgG conjugated to horseradish peroxidase for PPARα. Protein bands were revealed by an enhanced chemiluminescence reaction for PPARα. For immunohistochemical staining of PPARα, sections were incubated with the primary goat polyclonal antibody directed against PPARα at room temperature.RESULTS: Real-time PCR indicated that the PPARα level and expression level of CPT1A gene in hepatitis C patients lowered significantly as compared with the controls (1.8±2.8 vs 13±3.4, P = 0.0002; 1.1±1.5 vs 7.4+1, ,P = 0.004). Western blot results showed that the level of PPARα protein in the livers of hepatitis C patients was lower than that in controls (2.3±0.3 vs 3.6±0.2,P = 0.009). The immunohistochemical staining results in chronic hepatitis C patients indicated a decrease in PPARα staining in hepatocytes compared with those in the control livers. The in vitro studies found that in the N3 and N4 colon stably expressing HCV core protein, the PPARα mRNA levels were significantly lower than that in the controls.CONCLUSION: The impaired intrahepatic PPARα expression is associated with the pathogenic mechanism in hepatic injury during chronic HCV infection. HCV infection reduced the expression of PPARα and CPT1A at the level of not only mRNAs but also proteins. PPARα plays an important role in the pathogenesis of chronic HCV infection, but the impaired function of this nuclear receptor in HCV infection needs further studies. | Yang Cheng Sébastien Dharancy Mathilde Malapel Pierre Desreumaux | 2005 | World Journal of Gastroenterology2005，11，48： | 11 |
| 2 | Cytological and moleeular diagnosis of solid variant of papillary thyroid carcinoma: a case report显示文摘 | Troncone G Russo M Malapelle U | 2008 | CytoJouma]2008，5，： | 1 |
| 3 | UbcHIO overexpression in human lung carcinomas and its correlation with EGFR and p53 mutational status 显示文摘 | Pallante P Malapelle U Berlingieri MT | 2013 | Eur J Cancer2013，49，5： | 1 |
| 4 | Impaired expression of the peroxisome proliferator–activated receptor alpha during hepatitis C virus infection显示文摘 | Sébastien Dharancy Mathilde Malapel Gabriel Perlemuter Tania Roskams Yang Cheng Laurent Dubuquoy Philippe Podevin Filoména Conti Valérie Canva David Philippe Luc Gambiez Philippe Mathurin Jean-Claude Paris Kristina Schoonjans Yvon Calmus Stanislas Pol Joh | 2005 | Gastroenterology2005，，2： | 1 |
| 5 | EGFR mutations detected on cytology samples by a centralized laboratory reliably predict response to gefitinib in non-small cell lung carcinoma patients显示文摘 | Malapelle U Bellevicine C De Luca C | 2013 | Cancer Cytopathol2013，121，10： | 1 |
| 6 | Sphingosine ki- nase 1 overexpression contributes to eetuximah resistance in human colorectal cancer models显示文摘 | Rosa R Marciano R Malapelle U | 2013 | Clin Cancer Res2013，19，13： | 1 |
| 7 | EGFR and KRAS muta- tions detection on lung cancer liquid-based cytology: a pilot study 显示文摘 | Malapelle U de Rosa N Rocco D | 2012 | J Clin Pathol2012，65，1： | 1 |
| 8 | Sphingosine kinase 1 overexpression contributes to cetuximab resistance in human colorectal cancer models显示文摘 | Rosa R Marciano R Malapelle U | | 0，，1： | 1 |
| 9 | UbcH10 overex- pression in human lung carcinomas and its correlation with EGFR and p53 mutational status显示文摘 | Pallante P Malapelle U Bedingieri MT | 2013 | Eur J Cancer2013，49，5： | 1 |
| 10 | Cytology-based gene mutation tests to predict response to anti-epidermal growth factor receptor therapy : a review显示文摘 | Malapelle U Bellevieine C Zeppa P | 2011 | Diagn Cytopathol2011，39，9： | 1 |
| 11 | IL - 18 production down- stream of the Nlrp3 inflammasome confers protection against colorec- tal tumor formation 显示文摘 | Zaki M H Vogel P Body Malapel M | 2010 | The Journal of Immunology2010，185，8： | 1 |
| 12 | UbcH10 overexpression in human lung carcinomas and its correlation with EGFR and p53 mutational status显示文摘 | Pallante P Malapelle U Berlingieri M T | | 0，，12： | 1 |
| 13 | Protein hydrolysates stimulate proglucagon gene transcription in intestinal endocrine cells via two elements related to cyclic AMP response element显示文摘 | J.-C. Gevrey M. Malapel J. Philippe G. Mithieux J.-A. Chayvialle J. Abello M. Cordier-Bussat | 2004 | Diabetologia2004，，5： | 1 |
| 14 | UbcH10 overexpression in human lung carcinomas and its correla- tion with EGFR and p53 mutational status 显示文摘 | Pallante P Malapelle U Berlingieri MT | 2013 | Eur J Cancer2013，49，5： | 1 |
| 15 | EGFR mutations detected on cytology samples by a centralized laboratory reliably predict response to gefitinib in non-small cell lung carcinoma patients显示文摘 | Malapelle U Bellevicine C De Luca C | 2013 | Cancer Cytopathol2013，121，10： | 1 |
| 16 | Sphingosine kinase 1 overexpression contributes to cetuximab resistance in human colorectal cancer models显示文摘 | Rosa R Marciano R Malapelle U | 2013 | Clin Cancer Res2013，19，1： | 1 |
| 17 | BacterialRNA and small antiviral compounds activate caspase -1 throughcryopyrin/Nalp3显示文摘 | Kanneganti TD Ozoren N Body -Malapel M | 2006 | Nature2006，440，7081： | 1 |
| 18 | Ubc H10 overexpression in human lung carcinomas and its correlation with EGFR and p53 mutational status显示文摘 | Pallante P Malapelle U Berlingieri MT | 2013 | Eur J Cancer2013，49，5： | 1 |
| 19 | Multieentric encapsulated papillary oneoeytic neoplasm of the thyroid:A case diagnosed by a combined cytological,histological,immunohistochemieal,and molecu-lar approach 显示文摘 | Bellevicine C Malapelle U Docimo G | 2012 | Diagn Cytopatho12012，40，5： | 1 |
| 20 | NOD2: a potential target for regulating liver injury显示文摘 | Body Malapel M Dharancy S Berrebi D | 2008 | Lab Invest2008，88，3： | 1 |
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