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| 1 | Diarrhoea-predominant irritable bowel syndrome distinguishable by 16S rRNA gene phylotype quantification显示文摘AIM:To study whether selected bacterial 16S ribosomal RNA(rRNA)gene phylotypes are capable of disting- uishing irritable bowel syndrome(IBS). METHODS:The faecal microbiota of twenty volunteers with IBS,subdivided into eight diarrhoea-predominant (IBS-D),eight constipation-predominant(IBS-C)and four mixed symptom-subtype(IBS-M)IBS patients,and fifteen control subjects,were analysed at three time-points with a set of fourteen quantitative real-timepolymerase chain reaction assays.All assays targeted 16S rRNA gene phylotypes putatively associated with IBS,based on 16S rRNA gene library sequence analysis. The target phylotypes were affiliated with Actinobac-teria,Bacteroidetes and Firmicutes.Eight of the target phylotypes had less than 95%similarity to cultured bacterial species according to their 16S rRNA gene sequence.The data analyses were made with repeated-measures ANCOVA-type modelling of the data and principle component analysis(PCA)with linear mixed-effects models applied to the principal component scores. RESULTS:Bacterial phylotypes Clostridium cocleatum 88%,Clostridium thermosuccinogenes 85%,Coprobacillus catenaformis 91%,Ruminococcus bromii-like, Ruminococcus torques 91%,and R.torques 93%were detected from all samples analysed.A multivariate analysis of the relative quantities of all 14 bacterial 16S rRNA gene phylotypes suggested that the intestinal microbiota of the IBS-D patients differed from other sample groups.The PCA on the first principal component(PC1),explaining 30.36%of the observed variation in the IBS-D patient group,was significantly altered from all other sample groups(IBS-D vs control, P=0.01;IBS-D vs IBS-M,P=0.00;IBS-D vs IBS-C, P=0.05).Significant differences were also observed in the levels of distinct phylotypes using relative values in proportion to the total amount of bacteria.A phy- lotype with 85%similarity to C.thermosuccinogenes was quantified in significantly different quantities among the IBS-D and control subjects(-4.08±0.90 vs -3.33±1.16,P=0.04)and IBS-D and IBS-M subjects (-4.08±0.90 vs-3.08±1.38,P=0.05).Furthermore,a phylotype with 94%similarity to R.torques was more prevalent in IBS-D patients'intestinal micro- biota than in that of control subjects(-2.43±1.49 vs -4.02±1.63,P=0.01).A phylotype with 93%simi- larity to R.torques was associated with control sam- ples when compared with IBS-M(-2.41±0.53 vs -2.92±0.56,P=0.00).Additionally,a R.bromii-like phylotype was associated with IBS-C patients in com- parison to control subjects(-1.61±1.83 vs-3.69± 2.42,P=0.01).All of the above mentioned phylotype specific alterations were independent of the effect of time. CONCLUSION:Significant phylotype level alterationsin the intestinal microbiotas of IBS patients were observed,further emphasizing the possible contribution of the gastrointestinal microbiota in IBS. | Anna Lyra Teemu Rinttil Janne Nikkil Lotta Krogius-Kurikka Kajsa Kajander Erja Malinen Jaana Mtt Laura Mkel Airi Palva | 2009 | World Journal of Gastroenterology2009，15，47： | 26 |
| 2 | The number of vitamin D receptor binding sites defines the different vitamin D responsiveness of the CYP24 gene in malignant and normal mammary ceils显示文摘 | Matilainen JM Malinen M Turunen MM | 2010 | J Biol Chem2010，285，24： | 1 |
| 3 | LED-Based NIR Spectrometer Module for Hand-Held and Process Analyser Applications显示文摘 | Malinen J Kansakoski M Rikola R Eddison C G | 1998 | Sensor and Actuators B1998，51，13： | 1 |
| 4 | Nanofibrillar cellulose hydrogel promotes three-dimensional liver cell culture显示文摘 | Bhattacharya M Malinen MM Lauren P | 2012 | J Control Release2012，164，3： | 1 |
| 5 | The Fecal Microbiota of Irritable Bowel Syndrome Patients Differs Significantly From That of Healthy Subjects显示文摘 | Anna Kassinen Lotta Krogius-Kurikka Harri M?kivuokko Teemu Rinttil? Lars Paulin Jukka Corander Erja Malinen Juha Apajalahti Airi Palva | 2007 | Gastroenterology2007，，1： | 1 |
| 6 | Three members of the human pyruvate dehydrogenase kinase gene family are direct targets of the peroxisome proliferator-activated receptor beta/delta显示文摘 | Degenhardt T Saramaki A Malinen M | 2007 | J Mol Biol2007，372，2： | 1 |
| 7 | LED-Based NIR Spectrometer Module for Hand-Held and Process Analyser Applications 显示文摘 | Malinen J Kansakoski M Rikola R | 1998 | Sensor and Actuators B1998，51，13： | 1 |
| 8 | Comparison of gillnet and trawl in diurnal fish community sampling显示文摘 | Olin M Malinen T | | 0，，： | 1 |
| 9 | Gillnet catch in estimating the density and structure of fish community:Comparison of gillnet and trawl samples in a eutrophic lake显示文摘 | Olin M Malinen T Ruuhij(a)rvi J | | 0，，： | 1 |
| 10 | Improved differentiation of tactile activations in human secondary somatosensory cortex and thalamus using cardiactriggered Fmri显示文摘 | Malinen S Schu=rmann M Hlushchuk Y | 2006 | Exp Brain Res2006，174，： | 1 |
| 11 | Probiotic and milk technological properties of Lactob~illus brevis 显示文摘 | Ronka E Malinen E Saarela M | 2003 | International Journal of Food Microbiology2003，83，1： | 1 |
| 12 | Nanofibrillar cellulose hydrogel promotes three-dimensional liver cellculture显示文摘 | Bhattacharya M Malinen M M Lauren P | 2012 | Journal of Controlled Release2012，164，3： | 1 |
| 13 | K- means: clustering by gradual data transformation显示文摘 | MALINEN M FRANTI P | 2014 | Pattern Recognition2014，47，10： | 1 |
| 14 | Probiotic and Milk Technological Properties of Lactobacillus brevis显示文摘 | RONKA E MALINEN E SAARELA M | 2003 | International Journal of Food Microbiolog2003，83，： | 1 |
| 15 | LED-Based NIR spectrometer module for hand-held and progress analyzer applications显示文摘 | MALINEN J KANSAKOSKI M R EDDISON C G | 1998 | Sensor and Actuators B1998，51，13： | 1 |
| 16 | Regulation of the human cyclin C gene via multiple vitamin D3-responsive regions in its promoter显示文摘 | Sinkkonen L Malinen M Saavalainen K | 2005 | Nucleic Acids Res2005，33，8： | 1 |
| 17 | Peptide nanofiber hydrogel induces formation of bile canaliculi struc- tures in three-dimensional hepatic cell culture 显示文摘 | Malinen MM Palokangas H Yliperttula M | 2012 | Tissue Eng Part A2012，18，2324： | 1 |
| 18 | Cyclical regulation of the insulin-like growth factor binding protein 3 gene in re- sponse to la, 25-dihydroxy Vitamin D3显示文摘 | MALINEN M RYYNA J HEINA M | 2011 | Nucleic Acids Re- search2011，39，2： | 1 |
| 19 | A fast minimum spanning tree algorithm based on K-means显示文摘 | Zhong C M Malinen M Miao D Q | 2015 | Information Sciences2015，295，20： | 1 |
| 20 | On the direct estimation of creep and relaxation functions显示文摘 | Sorvari J Malinen M | 2007 | Mechanics of Time-dependent Materials2007，，11： | 1 |
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