|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Inhibiting effect of antisense oligonucleotides phosphorthioate on gene expression of TIMP-1 in rat liver fibrosis显示文摘AIM To observe the inhibition of antisenseoligonucleotides (asON) phosphorthioate to thetissue inhibitors metalloproteinase-1 (TIMP-1)gene and protein expression in the liver tissue ofimmunologically induced hepatic fibrosis rats.The possibility of reversing hepatic fibrosisthrough gene therapy was observed.METHODS Human serum albumin (HSA) wasused to attack rats, as hepatic fibrosis model, inwhich asONs were used to block the gene andprotein expressing TIMP-1. According to theanalysis of modulator, structure protein, codingseries of TIMP-1 genome, we designed fourdifferent asONs. These asONs were injected intothe hepatic fibrosis models through coccygealvein. The results was observed by RT-PCR formeasuring TIMP-1 mRNA expression,immunohistochemistry and in situ hybridizationfor collagen Ⅰ, Ⅲ, special staining of collagenfiber, and electron microscopic examination.RESULTS Hepatic fibrosis could last within 363days in our modified model. The expressinglevel of TIMP-1 was high during hepatic fibrosisprocess. It has been proved by theimmunohistochemical and the electronmicroscopic examination that the asONphosphorthioate of TIMP-1 could exactly expressin vivo. The effect of colchicine wasdemonstrated to inhibit the expressing level ofmRNA and the content of collagen Ⅰ, Ⅲ in theliver of experimental hepatic fibrosis rats.However, the electron microscopy research andthe pathologic grading of hepatic fibrosisshowed that there was no significant differencebetween the treatment group and the modelgroup (P>0.05).CONCLUSION The experimental rat model ofhepatic fibrosis is one of the preferable modelsto estimate the curative effect of anti-hepaticfibrosis drugs. The asON phosphorthioate ofTIMP-1 could block the gene and proteinexpression of TIMP-1 in the liver of experimentalhepatic fibrosis rats at the mRNA level. It ispossible to reverse hepatic fibrosis, and it isexpected to study a new drug of anti-hepaticfibrosis on the genetic level. Colchicine has verylimited therapeutic effect on hepatic fibrosis,furthermore, its toxicity and side effects areobvious. | Qing He Nie Yong Qian Cheng Yu Mei Xie Yong Xing Zhou Yi Zhan Cao The Center of Infectious Disease Diagnosis and Treatment of PLA,Tangdu Hospital,Forth Military Medical University,Xi’an 710038,Shaanxi Province,ChinaDr,Qing He Nie graduated from Qinghai Medical College as a doctor in 1983,got master degree at Beijing 302 Army Hospital in 1993,got doctor degree at the Third Military Medical University in 1998,engaged in postdoctoral research at the Fourth Military Medical University from 1998 to 2000,now an associate professor,specialized in clinical and experimental research of infectious diseases,had more than 90 papers published,coauthor of ten books,first author of one book. | 2001 | World Journal of Gastroenterology2001，7，3： | 73 |
| 2 | Overexpression of SOS (Salt Overly Sensitive) Genes Increases Salt Tolerance in Transgenic Arabidopsis显示文摘土壤咸度是减少种的一个主要不能生活的压力生长和生产率。最近,植物 overexpressing AtNHX1 或 SOS1 显著地增加了盐忍耐,这被报导。为了测试多重基因的 overexpression 是否能改善,甚至更种盐忍耐,我们生产了六不同转基因的 Arabidopsis 植物那 overexpress AtNHX1, SOS3, AtNHX1 ?+? SOS3, SOS1, SOS2 ?+? SOS3,或 SOS1 ?+? SOS2 ?+? SOS3。北污点分析在转基因的植物证实了相关基因抄本的高水平的存在。转基因的 Arabidopsis 植物 overexpressing AtNHX1 独自没在盐忍耐介绍任何重要增加,与更早的报告相反。我们发现那转基因的植物 overexpressing SOS3 展览增加了类似于植物 overexpressing SOS1 的盐忍耐。而且,转基因的植物 overexpressing AtNHX1 的盐忍耐 ?+? SOS3, SOS2 ?+? SOS3,或 SOS1 ?+? SOS2 ?+? 分别地, SOS3 独自显得类似于转基因的植物 overexpressing 任何一个 SOS1 或 SOS3 的忍耐。 | Qing Yang | 2009 | Molecular Plant2009，2，1： | 44 |
| 3 | Analysis of point mutation in site 1896 of HBV precore and its detection in the tissues and serum of HCC patients显示文摘INTRODUCTION Hepatitis B is one of the common infectious diseases,which severely impairs the health of the people in our country and has close relationship | Yuan Wang Hu Liu Qing Zhou Xu Li | 2000 | World Journal of Gastroenterology2000，6，3： | 33 |
| 4 | Baicalin prevents LPS-induced activation of TLR4/NF-κB p65 pathway and inflammation in mice via inhibiting the expression of CD14显示文摘Previous studies have shown that baicalin,an active ingredient of the Chinese traditional medicine Huangqin,attenuates LPS-induced inflammation by inhibiting the activation of TLR4/NF-κBp65 pathway,but how it affects this pathway is unknown.It has been shown that CD14 binds directly to LPS and plays an important role in sensitizing the cells to minute quantities of LPS via chaperoning LPS molecules to the TLR4/MD-2 signaling complex.In the present study we investigated the role of CD14 in the anti-inflammatory effects of baicalin in vitro and in vivo.Exposure to LPS(1μg/mL)induced inflammatory responses in RAW264.7 cells,evidenced by marked increases in the expression of MHC II molecules and the secretion of NO and IL-6,and by activation of MyD88/NF-κB p65 signaling pathway,as well as the expression of CD14 and TLR4.These changes were dose-dependently attenuated by pretreatment baicalin(12.5–50μM),but not by baicalin post-treatment.In RAW264.7 cells without LPS stimulation,baicalin dose-dependently inhibit the protein and mRNA expression of CD14,but not TLR4.In RAW264.7 cells with CD14 knockdown,baicalin pretreatment did not prevent inflammatory responses and activation of MyD88/NF-κB p65 pathway induced by high concentrations(1000μg/mL)of LPS.Furthermore,baicalin pretreatment also inhibited the expression of CD14 and activation of MyD88/NF-κB p65 pathway in LPS-induced hepatocyte-derived HepG2 cells and intestinal epithelial-derived HT-29 cells.In mice with intraperitoneal injection of LPS and in DSS-induced UC mice,oral administration of baicalin exerted protective effects by inhibition of CD14 expression and inflammation.Taken together,we demonstrate that baicalin pretreatment prevents LPS-induced inflammation in RAW264.7 cells in CD14-dependent manner.This study supports the therapeutic use of baicalin in preventing the progression of LPS-induced inflammatory diseases. | Ya-jun Fu Bo Xu Shao-wei Huang Xia Luo Xiang-liang Deng Shuang Luo Chang Liu Qing Wang Jin-yan Chen Lian Zhou | 2021 | Acta Pharmacologica Sinica2021，42，1： | 31 |
| 5 | Effect of hepatocyte apoptosis induced by TNF-α on acute severe hepatitis in mouse models显示文摘AIM To study the effect of hepatocyteapoptosis and necrosis induced by TNF-α on thepathogenesis of acute severe hepatitis(ASH).METHODS The model of ASH was prepared inD-galactosamine(GAIN)sensitized BALB/c miceby injection of either endotoxin(ET)or tumornecrosis factor-α(TNF-α).Morphologicalchanges of apoptotic hepatocytes were studiedby both light and electron microscope and in siteend labeling method(ISEL).Molecular biologicalchanges of DNA ladder were observed byelectrophoresis of extract from liver tissues.Biochemical changes were measured by alanineaminotransferase(ALT),asparticaminotransferase(AST)and TNF-α.The relationbetween apoptosis and necrosis was evaluatedsimultaneously.RESULTS The sequence of hepatocyteapoptosis,necrosis,and final death from ASHwas observed both in GAIN/ET and GAIN/TNF-agroup.Apoptosis was prominent at 3.5 h and 5 hafter injection of inducer,while necrosis becamedominant at 9 h after challenge.The appearanceof apoptosis was earlier in GAIN/TNF-α groupthan that in GAIN/ ET group.Pretreatment ofmice with antiTNF IgG1 may completely preventthe liver injury induced by GalN/ET.CONCLUSION TNF-α can cause liver damageby inducing hepatic apoptosis and necrosis inmice with endotoxemia. | Guo Qing Zang Xia Qiu Zhou Hong Yu Qing Xie Guo Ming Zhao Bin Wang Qing Guo Yue Qin Xiang Dan Liao Department of Infectious Diseases,Rujin Hospital,Shanghai Second Medical University,Shanghai 200025,China | 2000 | World Journal of Gastroenterology2000，6，5： | 30 |
| 6 | Wnt/β-catenin signalling:function,biological mechanisms,and therapeutic opportunities显示文摘The Wnt/β-catenin pathway comprises a family of proteins that play critical roles in embryonic development and adult tissue homeostasis.The deregulation of Wnt/β-catenin signalling often leads to various serious diseases,including cancer and non-cancer diseases.Although many articles have reviewed Wnt/β-catenin from various aspects,a systematic review encompassing the origin,composition,function,and clinical trials of the Wnt/β-catenin signalling pathway in tumour and diseases is lacking.In this article,we comprehensively review the Wnt/β-catenin pathway from the above five aspects in combination with the latest research.Finally,we propose challenges and opportunities for the development of small-molecular compounds targeting the Wnt signalling pathway in disease treatment. | Jiaqi Liu Qing Xiao Jiani Xiao Chenxi Niu Yuanyuan Li Xiaojun Zhang Zhengwei Zhou Guang Shu Gang Yin | 2022 | Signal Transduction and Targeted Therapy2022，7，2： | 28 |
| 7 | Eco-toxicity of petroleum hydrocarbon contaminated soil显示文摘Total petroleum hydrocarbons (TPH) contaminated soil samples were collected from Shengli Oilfield of China. Toxicity analysis was carried out based on earthworm acute toxicity, plant growth experiment and luminescent bacteria test. The soil was contaminated by-petroleum hydrogcarbons with TPH concentration of 10.57%. With lethal and sub-lethal rate as endpoint, earthworm test showed that the LD 50 (lethal dose 50%) values in 4 and 7 days were 1.45% and 1.37% respectively, and the inhibition rate of earthworm body weight increased with higher oil concentration. TPH pollution in the soil inhibited seed germination in both wheat and maize experiment when the concentration of petroleum was higher than 0.1%. The EC 50 (effective concentration 50%) for germination is 3.04% and 2.86% in maize and wheat, respectively. While lower value of EC 50 for root elongation was to be 1.11% and 1.64% in maize and wheat, respectively, suggesting higher sensitivity of root elongation on petroleum contamination in the soil. The EC 50 value in luminescent bacteria test was 0.47% for petroleum in the contaminated soil. From the experiment result, it was concluded that TPH content of 1.5% is considered to be a critical value for plant growth and living of earthworm and 0.5% will affect the activity of luminescent bacteria. | Jingchun Tang Min Wang Fei Wang Qing Sun Qixing Zhou | 2011 | Journal of Environmental Sciences2011，23，5： | 26 |
| 8 | Preliminary characterization of acute aortic dissection in the mainland of China显示文摘经济地在更少和更多之中在尖锐大动脉的解剖( AAD )的特征探索差别的目的从尖锐大动脉的解剖( IRAD )的国际注册表与各种各样的文化和赛跑数据来源报告开发了国家,中国( MC )的大陆由寻找 PubMed 数据库是镇定的并且从2000年1月的中国杂志全文的数据库到2009年3月 .Study 选择从包含大量案例和 comple 的 IRAD 和 MC 的那些报告并且从包含病人们在 MC 由一样的医学中心报导了的最大的数字的文章的数据从 MC 被拿进 statistics.Results AAD 病人是比 IRAD 国家和在中国组的男病人的百分比显著地年轻的比 IRAD 国家高(80.7%对68.6%, P 0.001 )。在 MC 的病人多半是更少除了任何焦点的神经病学的赤字与典型症状和符号介绍。与 IRAD 组,不同中国病人是容易的经历磁性的回声成像(MRI ) 做 AAD 的诊断(45.5%vs.11.6% , P 0.001 ) 。在里面医院死亡在 2 个组之间是类似的,但是在 MC 的 AAD 病人的仅仅更小的比例经历了外科或在 MC 的病人被给看的 AAD 和在在 MC 和 IRAD 之间的一些临床的变量的差别的一般特征组织的 medicaltreatment.Conclusions 仍然存在。 | WANG Dong-jin FAN Fu-dong WANG Qiang LI Qing-guo ZHOU Qing WU Zhong SHI Guang-fei | 2011 | Chinese Medical Journal2011，，11： | 23 |
| 9 | Analysis on Factors Related to Rabies Epidemic in China from 2007-2011显示文摘To analyze features of the rabies epidemic in China between 2007 and 2011, identify factors influencing the epidemic and to provide a scientific basis for further control and prevention of rabies, Descriptive epidemiological methods and statistical analysis was used on data collected from the National Disease Reporting Information System between 2007 to 2011 and the National Active Surveillance System between 2007 and 2010. Our analysis shows that while the number of human rabies cases decreased year by year, the number of districts reporting cases did not show significant change. The situations in Guangdong, Guangxi, Guizhou and Hunan provinces clearly improved over the period but they remain provinces with high-incidence, and consequently influence the epidemic situation of surrounding provinces and possibly the whole country. Summer and autumn were high-incidence seasons. Farmers, students and pre-school children represent the high-risk populations, and rates of cases in farmers increased, those for students decreased, and pre-school children remained unchanged. Provinces with active surveillance programs reported a total of 2346 individual cases, of which 88.53% were associated with canines. Postexposure prophylaxis (PEP) of rabies cases was not significantly improved, whereas PEP in post-exposure population was good. In rural regions of China, canine density was reduced somewhat, and the immunization rate increased slightly. Finally we show that while the epidemic decreased 2007 to 2011 in China, cases continued to be diffused in certain regions. Lack of standardization of PEP on rabies cases was the main reason of morbidity. The high density and low immunization of dog in rural areas and the defective situation of PEP are still continuous occurrences in China and remain a cause for concern. | Cui-ping Yin Hang Zhou Hui Wu Xiao-yan Tao Simon Rayner Shu-mei Wang Qing Tang Guo-dong Liang | 2012 | Virologica Sinica2012，27，2： | 22 |
| 10 | Sequencing of Cultivated Peanut, Arachis hypogaea, Yields Insights into Genome Evolution and Oil Improvement显示文摘Cultivated peanut (Arachis hypogaea) is an allotetraploid crop planted in Asia, Africa, and America for edible oil and protein. To explore the origins and consequences of tetraploidy, we sequenced the allotetraploid A. hypogaea genome and compared it with the related diploid Arachis duranensis and Arachis ipaensis genomes. We annotated 39 888 A-subgenome genes and 41 526 B-subgenome genes in allotetraploid peanut. The A. hypogaea subgenomes have evolved asymmetrically, with the B subgenome resembling the ancestral state and the A subgenome undergoing more gene disruption, loss, conversion, and transposable element proliferation, and having reduced gene expression during seed development despite lacking genome-wide expression dominance. Genomic and transcriptomic analyses identified more than 2 500 oil metabolism-related genes and revealed that most of them show altered expression early in seed development while their expression ceases during desiccation, presenting a comprehensive map of peanut lipid biosynthesis. The availability of these genomic resources will facilitate a better understanding of the complex genome architecture, agronomically and economically important genes, and genetic improvement of peanut. | Xiaoping Chen Qing Lu Hao Liu Jianan Zhang Yanbin Hong Haofa Lan Haifen Li Jinpeng Wang Haiyan Liu Shaoxiong Li Manish K.Pandey Zhikang Zhang Guiyuan Zhou Jigao Yu Guoqiang Zhang Jiaqing Yuan Xingyu Li Shijie Wen Fanbo Meng Shanlin Yu Xiyin Wang Kadambot H.M.Siddique Zhong-Jian Liu Andrew H.Paterson Rajeev K.Varshney Xuanqiang Liang | 2019 | Molecular Plant2019，12，7： | 23 |
| 11 | Aerosolized Amikacin as Adjunctive Therapy of Ventilator-associated Pneumonia Caused by Multidrug-resistant Gram-negative Bacteria: A Single-center Randomized Controlled Trial显示文摘 | Chang Liu Yu-Ting Zhang Zhi-Yong Peng Qing Zhou Bo Hu Hui Zhou Jian-Guo Li | 2017 | Chinese Medical Journal2017，，10： | 20 |
| 12 | Impact of CYP2C19 polymorphism and smoking on response to clopidogrel in patients with stable coronary artery disease显示文摘有阿司匹林和 clopidogrel 的背景双反血小板治疗是为经历经皮的冠的干预(一种总线标准) 阻止 thrombotic 事件的病人的确定的基础。现在的学习被进行检验 CYP2C19 681GA 多型性并且香烟吸烟是否在由于稳定的心绞痛经历选任的冠的 stent 放置的 722 个中国汉病人之中在对 clopidogrel.Methods 的反应上有独立或交互的效果, 300 mg clopidogrel 的装载剂量被给所有病人和 75 mg 的每日的维护剂量最少 12 个月。CYP2C19 681GA 多型性是 genotyped。血小板反应被轻发射度 aggregometry (LTA ) 与导致的 5 mol/L 腺苷 diphosphate (自动数据处理) 测量。差的反应被定义为 10% 或少些在在装载结果显示出的 clopidogrel.Results 的剂量以后的基线和 24 个小时的聚集之间的绝对差别对 clopidogrel 差反应在 105 个病人(14.5%) 被介绍。总的来说,遗传型 GA/AA 搬运人是可能的是穷人应答的盒子(19.6% 对 11.0% , P=0.001 ) 与机会比率(或) 1.971 (95% CI:1.296-2.998, P=0.002 ) ,与 GG 同质接合体相比。与不抽烟的人相比,同时,吸烟者显示出更低的率差反应(10.9% 对 17.3% , P=0.015 ) 与或 0.582 (95% CI:0.374-0.904, P=0.016 ) 。有 GG 遗传型的吸烟者有最低风险与或 0.487 (95% CI:0.246-0.961, P=0.038 ) 当有 GA/AA 遗传型的不抽烟的人有最高的风险时与或 1.823 差反应(95% CI:1.083-3.068, P=0.024 ) ,与有 GG 遗传型的不抽烟的人相比。然而,在我们的学习显示了的遗传型和 smoking.Conclusion 之间没有重要相互作用那两个 CYP2C19 多型性和吸烟独立地影响的反应到 clopidogrel。 | LIU Xiao-li WANG Zhi-jian YANG Qing GE Hai-long GAO Fei LIU Yu-yang SHI Dong-mei ZHAO Ying-xin ZHOU Yu-jie | 2010 | Chinese Medical Journal2010，，22： | 19 |
| 13 | Application of an acrylic vessel supported by a stainless-steel truss for the JUNO central detector显示文摘在 Daya 海湾实验的成功以后, Jiangmen 地下的中微子天文台(Jupiter 之妻) 被发射测量中微子团层次和摆动参数并且学习另外的中微子物理。它的中央察觉者从反应堆,地球,空气,和阳光被放为反中微子。中央察觉者的主要要求是液体闪烁器的 20 kt 的抑制,作为目标团,和 3% 精力分辨率。它关于有它的内部表面上的 PMT 的 75% 范围的 38.5 m 的一个球形状察觉者。因为它必须为物理测量的几种不同类型满足要求并且在它的结构和工程拥有可行性和可靠性,如此的一个巨大的察觉者的设计是大挑战,都在合理时间和费用。为 JUNO 中央察觉者的一种选择是超丙烯酸的球在水里沉没了防护背景。这份报纸为如此的一个丙烯酸的球建议一个结构的计划那被支持由一不锈钢捆绑,在土木工程由支持点的眼镜窗帘墙启发了。计划的初步的设计被有限元素(FE ) 用 ABAQUS 完成并且验证方法。FE 分析证明计划能在 5 ~ 10 MPa 的限制以内控制丙烯酸的球的压力水平,根据中央察觉者的设计目的需求。计划具有突出的全球稳定性并且在本地连接上允许各种各样的选择。我们证明计划可行性好并且应该是为中央察觉者的一种合理选择。 | WANG Yuan Qing ZONG Liang HENG Yue Kun WANG Zong Yi ZHOU Yan HOU Shao Jing QIN Zhong Hua MA Xiao Yan | 2014 | Science China(Technological Sciences)2014，57，12： | 19 |
| 14 | The Genome of Medicinal Plant Macleaya cordata Provides New Insights into Benzylisoquinoline Alkaloids Metabolism显示文摘在动物农业和药的抗菌素的 overuse 引起了一系列潜在的威胁到公共健康。Macleaya cordata 是从 Papaveraceae 家庭的药用的植物种,提供为抗菌剂的制造的一个安全资源为家畜喂添加剂。从 M. 的活跃成分 ? cordata 被知道包括 benzylisoquinoline 碱(偏爱) 象 sanguinarine (圣) 和 chelerythrine (CHE ) 那样的 ?,而是他们的新陈代谢的小径还得在这非模型植物被学习。在 M. 的圣和 CHE 的活跃生合成 ? cordata 被喂 13标记 C 的酷氨酸。为了增加,推进卓见,我们 de novo 定序 M 的整个染色体。cordata,第一从 Papaveraceae 家庭被定序。M.? 盖住 378 Mb 的 cordata 染色体与 43.5%being 编码 22,328 预言的编码蛋白质的基因 transposable 元素。作为基础 eudicot 的一个成员, M.? cordata 染色体缺乏 ? 发生在几乎所有 eudicots 的 paleohexaploidy 事件。从 genomics 数据, 16 的一个完全的集合 ? 新陈代谢 ? 为圣和 CHE 生合成的基因被检索,并且 14 项他们的生物化学的活动被验证。这些 genomics 和新陈代谢的数据在 M. 显示出保存 BIA 新陈代谢的小径 ? cordata 并且由庄稼改进或微生物引起的小径重建为圣和 CHE 的未来生产提供知识基础。 | Xiubin Liu Yisong Liu Peng Huang Yongshuo Ma Zhixing Qing Qi Tang Huifen Cao Pi Cheng Yajie Zheng Zejun Yuan Yuan Zhou Jinfeng Liu Zhaoshan Tang Yixiu Zhuo Yancong Zhang Linlan Yu Jialu Huang Peng Yang Qiong Peng dinbo Zhang Wenkai Jiang Zhonghua Zhang Kui Lin Dae-Kyun Ro Xiaoya Chen Xingyao Xiong Yi Shang Sanwen Huang Jianguo Zeng | 2017 | Molecular Plant2017，10，7： | 19 |
| 15 | From concept to reality-A review to the primary test stand and its preliminary application in high energy density physics显示文摘Pulsed power technology,whereas the electrical energy stored in a relative long period is released in much shorter timescale,is an efficient method to create high energy density physics(HEDP)conditions in laboratory.Around the beginning of this century,China Academy of Engineering Physics(CAEP)began to build some experimental facilities for HEDP investigations,among which the Primary Test Stand(PTS),a multi-module pulsed power facility with a nominal current of 10 MA and a current rising time~90 ns,is an important achievement on the roadmap of the electro-magnetically driven inertial confinement fusion(ICF)researches.PTS is the first pulsed power facility beyond 10 TW in China.Therefore,all the technologies have to be demonstrated,and all the engineering issues have to be overcome.In this article,the research outline,key technologies and the preliminary HEDP experiments are reviewed.Prospects on HEDP research on PTS and pulsed power development for the next step are also discussed. | Jianjun Deng Weiping Xie Shuping Feng Meng Wang Hongtao Li Shengyi Song Minghe Xia Ji Ce An He Qing Tian Yuanchao Gu Yongchao Guan Bin Wei Xianbin Huang Xiaodong Ren Jiakun Dan Jing Li Shaotong Zhou Hongchun Cai Siqun Zhang Kunlun Wang Qiang Xu Yujuan Wang Zhaohui Zhang Guilin Wang Shuai Guo Yi He Yiwei Zhou Zhanji Zhang Libing Yang Wenkang Zou | 2016 | Matter and Radiation at Extremes2016，1，1： | 19 |
| 16 | Hepatitis C virus infections and genotypes in China显示文摘Objective: To define the conditions of hepatitis C vi-rus (HCV) infections, and geographic and demo-graphic distributions of genotypes in China.Methods: HCV infected patients were selected fromindividuals with different patterns of liver diseasesand high risk populations in different parts of China.Genotypes of HCV in some isolates were further ana-lyzed, based on the data from our laboratory studiesand some carefully selected published literatures.Results: The anti-HCV positive rates were 9.7% inpatients with acute hepatitis, 13.3% in patients withchronic hepatitis, 18.3% in patients with hepatocel-lular carcinoma, 33.0% in patients with liver cirrho-sis, and 43.2% in patients with posttransfusionalhepatitis (average, 16.2% in patients with liver di-seases). The anti-HCV positive rates in the high riskpopulations were 36.4% in leukemic patients,43.0% in hemodialysis patients, 12.7% in blood do-nors, 64.1% in drug abusers, 13.1% in prostitutes,and 2.57% in naturally healthy people. At least 4clades (clades 1, 2, 3 and 6) of HCV were found inChina with different geographic and demographicdistributions. Genotype 1b was the most widely dis-tributed genotype, and genotype 3 was mainly foundin Yunnan Province, Southwest China.Conclusion: China has a high incidence of HCV in-fection. Our results will provide a strategic basis fordiagnosis, treatment and possibly prophylaxis of he-patitis C virus diseases. | Yuan-Ding Chen Ming-Ying Liu Wen-Lin Yu Jia-Qi Li Mei Peng Qing Dai Xiao Liu Zhen-Quan Zhou From the Central Laboratory, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, China Kunming Hospital for Infectious Diseases, Kunming 650041, China | 2002 | Hepatobiliary & Pancreatic Diseases International2002，1，2： | 18 |
| 17 | Nedaplatin/Gemcitabine Versus Carboplatin/Gemcitabine in Treatment of Advanced Non-small Cell Lung Cancer: A Randomized Clinical Trial显示文摘Objective: To evaluate the efficacy and safety of nedaplatin/gemcitabine (NG) and carboplatin/gemcitabine (CG) in the management of untreated advanced non‐small cell lung cancer (NSCLC). Methods: Sixty‐two patients with previously untreated advanced NSCLC were recruited between June 2006 and November 2007. Subjects were randomly assigned to the NG arm (n=30) and the CG arm (n=32). Only patients (24 and 25 in the NG and CG arms, respectively) who completed ≥2 chemotherapy cycles were included in the data analysis. The primary outcome measure was the objective response rate (ORR). The secondary outcome measures included progression‐free survival (PFS), overall survival (OS) and adverse events. Results: There were no statistically significant differences in the efficacy measures (ORR, P=0.305; median PFS, P=0.198; median OS, P=0.961) or in the major adverse events (grade 3/4 neutropenia, P=0.666; grade 3/4 anemia, P=0.263; grade 3/4 thrombocytopenia, P=0.212) between the two treatment arms. However, there was a trend towards higher ORR (37.5% vs. 24.0%), longer PFS (6.0 vs. 5.0 months), and less adverse events in the NG arm. Conclusion: NG regimen seems to be superior over CG regimen for advance NSCLS, but further investigation is needed to validate this superiority. | Jin-ji Yang Qing Zhou Ri-qiang Liao Yi-sheng Huang Chong-rui Xu Zhen Wang Bin-chao Wang Hua-jun Chen Yi-long Wu | 2012 | Chinese Journal of Cancer Research2012，24，2： | 18 |
| 18 | Organic anion transporting polypeptide-1B1 haplotypes in Chinese patients显示文摘瞄准:在搬运 polypeptide-1B1 的器官的阴离子检测 388G >A 和 521T >C 变体等位基因(OATP1B1,编码基因 SLCO1B1 ) 基因。方法:111 个健康志愿者在我们的学习为 OATP1B1 等位基因被屏蔽。PCR 限制碎片长度多型性被用来鉴别 388G >A 多型性和一个 1 步 tetra 教材方法为 521T >C 变化的决心被开发。结果:在中国人口的 388G >A 和 521T >C 变体等位基因的频率分别地是 73.4% 和 14.0% 。SLCO1B1 *1b 和 *15 haplotypes 的频率分别地是 59.9% 和 14.0% 。结论:SLCO1B1 *1b 和 SLCO1B1 *15 变体在中国人口是相对普通的。他们的频率类似于那在从那在白种人和黑人日本,却显著地不同。 | Lin-yong XU Yi-jing HE Wei ZHANG Sheng Deng Qing LI Wei-xia ZHANG Zhao-qian LIU Dan WANG Yuan-fei HUANG Hong-hao ZHOU Zhen-qiu SUN | 2007 | Acta Pharmacologica Sinica2007，28，10： | 18 |
| 19 | Molecular hydrogen regulates the expression of miR-9,miR-21 and miR-199 in LPS-activated retinal microglia cells显示文摘AIM: To explore the potential mechanism of molecular hydrogen in the regulation of miRNA expression and signal-modulating activities. ·METHODS: Retinal microglia cells were activated by Lipopolysaccharides (LPS) and then treated with hydrogen -saturated medium or normal medium without hydrogen. qRT -PCR was used to detect the expression difference in miR-9, miR-21 and miR-199 between these two groups. Moreover, the expression of LPS -induced signaling proteins, including Myd88, IKK -β, NF -kB, and PDCD4, were detected by Western blotting. ·RESULTS: The results demonstrated a marked down- regulation of miR -9 and miR -21 and up -regulation of miR-199 by hydrogen treatment; the expression of Myd88 and IKK-β was decreased after hydrogen treatment, whereas PDCD4 was increased, and there was no significant change in NF-kB expression. · CONCLUSION: The results in the present study indicate that miR -9, miR -199 and miR -21 play an important role in the anti -inflammatory regulation of LPS -activated microglia cells by molecular hydrogen, which will help to explain the protective mechanism of molecular hydrogen against inflammatory injury. | Guo-Dan Liu Hong Zhang Lin Wang Qing Han Shi-Feng Zhou Ping Liu | 2013 | International Journal of Ophthalmology(English edition)2013，6，3： | 17 |
| 20 | Haploinsufficiency of A20 (HA20): updates on the genetics, phenotype, pathogenesis and treatment显示文摘Background A20,a protein encoded by the tumor necrosis factor alpha-induced protein 3 gene(TNFAIP3),plays a vital role in the negative regulation of inflammation and immunity.Loss-of-function mutation in TNFAIP3 leads to a new described autoinflammatory disease-haploinsufficiency of A20(HA20).Since HA20 was first described in 2016,a number of new cases have been described in this literature,however,the disease and its pathogenesis are poorly understood.This review seeks to improve clinical recognition of this disorder,and promote both earlier diagnosis and initiation of targeted therapies to improve patients’outcomes.Methods We reviewed 26 papers about A20 and HA20,and we summarized genetic variants and clinical manifestations of a total of 61 reported patients from 26 families identified to have a genetic diagnosis of germline pathogenic variants in TNFAIP3/A20.Additionally,we discussed the pathogenesis and treatment of HA20.Results A total of 24 pathogenic variants of A20 had been reported.There was significant clinical heterogeneity,even among those with the same variants in TNFA1P3.Prior to receiving a molecular diagnosis of HA20,patients had been diagnosed with Behcet's disease,rheumatoid arthritis,rheumatic fever,juvenile idiopathic arthritis,systemic lupus erythematosus,and even adult-onset Stills'disease.The patients with HA20 that presented with inflammatory signatures in NF-kB signaling were mostly responsive to treatment.Conclusions HA20 is a monogenic autoinflammatory disease with highly variable clinical manifestations.This extensive heterogeneity makes it difficult to set a clinical diagnostic criteria,and genetic sequencing is necessary for a definitive diagnosis of HA20. | Mei-Ping Yu Xi-Sheng Xu Qing Zhou Natalie Deuitch Mei-Ping Lu | 2020 | World Journal of Pediatrics2020，16，6： | 17 |
您所在的机构暂未开通此部分的功能，请联系管理员