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| 1 | Single-cell transcriptomic analysis defines the interplay between tumor cells,viral infection,and the microenvironment in nasopharyngeal carcinoma显示文摘Nasopharyngeal carcinoma(NPC)is an Epstein-Barr virus(EBV)-associated malignancy with a complex tumor ecosystem.How the interplay between tumor cells,EBV,and the microenvironment contributes to NPC progression and immune evasion remains unclear.Here we performed single-cell RNA sequencing on^104,000 cells from 19 EBV^+NPCs and 7 nonmalignant nasopharyngeal biopsies,simultaneously profiling the transcriptomes of malignant cells,EBV,stromal and immune cells.Overall,we identified global upregulation of interferon responses in the multicellular ecosystem of NPC.Notably,an epithelial–immune dual feature of malignant cells was discovered and associated with poor prognosis.Functional experiments revealed that tumor cells with this dual feature exhibited a higher capacity for tumorigenesis.Further characterization of the cellular components of the tumor microenvironment(TME)and their interactions with tumor cells revealed that the dual feature of tumor cells was positively correlated with the expression of co-inhibitory receptors on CD8^+tumor-infiltrating T cells.In addition,tumor cells with the dual feature were found to repress IFN-γproduction by T cells,demonstrating their capacity for immune suppression.Our results provide new insights into the multicellular ecosystem of NPC and offer important clinical implications. | Shanzhao Jin Ruoyan Li Ming-Yuan Chen Chao Yu Lin-Quan Tang Yan-Min Liu Jiang-Ping Li Yi-Na Liu Yi-Ling Luo Yifan Zhao Yu Zhang Tian-Liang Xia Shang-Xin Liu Qi Liu Guan-Nan Wang Rui You Jing-Yun Peng Jiang Li Feng Han Jianwei Wang Qiu-Yan Chen Li Zhang Hai-Qiang Mai Benjamin E.Gewurz Bo Zhao Lawrence S.Young Qian Zhong Fan Bai Mu-Sheng Zeng | 2020 | Cell Research2020，30，11： | 16 |
| 2 | Discovery of biclonal origin and a novel oncogene SLC12A5 in colon cancer by single-cell sequencing显示文摘单个房间的定序是为描出的一个强大的工具为个性化的癌症管理的同种细胞的关系和识别关键司机基因。这里,我们执行了结肠癌的一个盒子的单个房间的定序的分析。人口遗传分析在肿瘤房间人口识别了二独立克隆。主要肿瘤克隆作为早 oncogenic 事件怀有 APC 和 TP53 变化,而次要的克隆包含了优势的 CDC27 和 PABPC1 变化。在这二克隆的次要的克隆支持的 APC 和 TP53 变化的缺席从二细胞的起源被导出。体的变化等位基因频率系列的检查另外 21 个整个织物的定序 exome 的盒子在结肠癌揭示了同种细胞的起源的异质。下次,我们识别了在单个房间的水平显示出变化的高频率,但是在人口水平展出了低流行的变异的基因 SLC12A5。变异的 SLC12A5 的功能的描述在结肠癌揭示了它的潜在的 oncogenic 效果。我们的学习在单个房间的水平支持提供第一条 exome 宽的证据那结肠癌能具有 biclonal 起源,并且建议在一个队的低流行的变化可以也在单个水平起重要 protumorigenic 作用。 | Chang Yu Jun Yu Xlaotlan Yao Wllham KK Wu Youyong Lu Senwel Tang Xiangchun Li Li Bao Xiaoxing Li Yong Hou Renhua Wu Min Jian Ruoyan Chen Fan Zhang Lixia Xu Fan Fan Jun He Qiaoyi Liang Hongyi Wang Xueda Hu Minghui He Xiang Zhang Hancheng Zheng Qibin Li Hanjie Wu Yan Chen Xu Yang Shida Zhu Xun Xu Huanming Yang Jian Wang Xiuqing Zhang Joseph JY Sung Yingrui Li Jun Wang | 2014 | Cell Research2014，，6： | 16 |
| 3 | The antipsychotic aripiprazole is a potent, partial agonist at the human 5-HT1A re- ceptor显示文摘 | Jordan S Koprivica V Chen Ruoyan | 2002 | Eur J Pharmacol2002，441，3： | 1 |
| 4 | The antipsychotic aripiprazole is a potent, partial agonist at the human 5-HT 1A receptor显示文摘 | Shaun Jordan Vuk Koprivica Ruoyan Chen Katsura Tottori Tetsuro Kikuchi C.Anthony Altar | 2002 | European Journal of Pharmacology2002，，3： | 1 |
| 5 | The antipsychoticaripiprazole is a potent, partial agonist at the human 5-HT1A receptor显示文摘 | Jordan S Koprivica V Chen Ruoyan | 2002 | Eur J Pharmacol2002，441，3： | 1 |
| 6 | Effects of electroconvulsive seizures and antidepressant drugs on brain-derived neurotrophic factor protein in rat brain显示文摘 | C.Anthony Altar Richard E. Whitehead Ruoyan Chen Gitta W?rtwein Torsten M. Madsen | 2003 | Biological Psychiatry2003，，7： | 1 |
| 7 | SIMULTANEOUS DETECTION OF ETHYL CARBAMATE AND UREA IN CHINESE YELLOW RICE WINE BY HPLC-FLD显示文摘 | Ruoyan Wang Hongmiao Wu Xiaoping Zhou Li Chen | 2014 | Journal of Liquid Chromatography & Related Technologies2014，，1： | 1 |
| 8 | Single-cell DNA methylome analysis of circulating tumor cells显示文摘Objective: Previous investigations of circulating tumor cells(CTCs) have mainly focused on their genomic or transcriptomic features, leaving their epigenetic landscape relatively uncharacterized. Here, we investigated the genome-wide DNA methylome of CTCs with a view to understanding the epigenetic regulatory mechanisms underlying cancer metastasis.Methods: We evaluated single-cell DNA methylome and copy number alteration(CNA) in 196 single cells,including 107 CTCs collected from 17 cancer patients covering six different cancer types. Our single-cell bisulfite sequencing(sc BS-seq) covered on average 11.78% of all Cp G dinucleotides and accurately deduced the CNA patterns at 500 kb resolution.Results: We report distinct subclonal structures and different evolutionary histories of CTCs inferred from CNA and DNA methylation profiles. Furthermore, we demonstrate potential tumor origin classification based on the tissue-specific DNA methylation profiles of CTCs.Conclusions: Our work provides a comprehensive survey of genome-wide DNA methylome in single CTCs and reveals 5-methylcytosine(5-m C) heterogeneity in CTCs, addressing the potential epigenetic regulatory mechanisms underlying cancer metastasis and facilitating the future clinical application of CTCs. | Hengyu Chen Zhe Su Ruoyan Li Ning Zhang Hua Guo Fan Bai | 2021 | Chinese Journal of Cancer Research2021，33，3： | 0 |
| 9 | Single-cell transcriptomics of immune cells in lymph nodes reveals their composition and alterations in functional dynamics during the early stages of bubonic plague显示文摘Bubonic plague caused by Yersinia pestis is highly infectious and often fatal.Characterization of the host immune response and its subsequent suppression by Y.pestis is critical to understanding the pathogenesis of Y.pestis.Here,we utilized single-cell RNA sequencing to systematically profile the transcriptomes of immune cells in draining lymph nodes(d LNs)during the early stage of Y.pestis infection.Dendritic cells responded to Y.pestis within 2 h post-infection(hpi),followed by the activation of macrophages/monocytes(Mφs/Mons)and recruitment of polymorphonuclear neutrophils(PMNs)to d LNs at 24 hpi.Analysis of cell-to-cell communication suggests that PMNs may be recruited to lymph nodes following the secretion of CCL9 by Mφs/Mons stimulated through CCR1-CCL9 interaction.Significant functional suppression of all the three innate immune cell types occurred during the early stage of infection.In summary,we present a dynamic immune landscape,at single-cell resolution,of murine d LNs involved in the response to Y.pestis infection,which may facilitate the understanding of the plague pathogenesis of during the early stage of infection. | Yifan Zhao Tong Wang Ziyang Liu Yuehua Ke Ruoyan Li Hongyan Chen Yang You Gengshan Wu Shiyang Cao Zongmin Du Fan Bai Ruifu Yang | 2023 | Science China(Life Sciences)2023，66，1： | 0 |
| 10 | Electromagnetic Force Balanced Single-Wheel Robot显示文摘It is a challenging task to carry on the research concerning the lateral stabilization of Single wheel robot(SWR). The lateral dynamics of earlier flywheel stabilizing SWR is derived to explain the shortcomings of such a mechanism, and the recovery torque proportional to the acceleration and moment of inertia of the flywheel is proved. But as the motor being a speed server system,it is difficult to control its acceleration to actuate the flywheel to provide recovery torque for SWR; and the moment of inertia of the flywheel must be large enough to produce adequate recovery torque, which makes such a system rather cumbersome. We proposed a new mechanism to solve the problem by introducing an electromagnetic force. The proposed mechanism is described briefly, and the dynamic analyses of such a new SWR is given, then the stability analysis is also done. Three-dimensional(3D)multi-body simulation, numerical simulation and physical invented pendulum prototype experiments were conducted to verify the proposed mechanism. The experiment results verified that the proposed mechanism is feasible and have some advantages over the flywheel balanced SWR. | ZHU Xiaoqing RUAN Xiaogang CHEN Zhigang WEI Ruoyan XIAO Yao | 2016 | Chinese Journal of Electronics2016，25，3： | 0 |
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