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| 1 | Development of highly glyphosate-tolerant tobacco by coexpression of glyphosate acetyltransferase gat and EPSPS G2-aroA genes显示文摘The widely used herbicide glyphosate targets 5-enolpyruvylshikimate-3-phosphate synthase(EPSPS).Glyphosate acetyltransferase(GAT)effectively detoxifies glyphosate by N-acetylation.With the aim of identifying a new strategy for development of glyphosate-tolerant crops,the plant expression vector pG2-GAT harboring gat and G2-aroA(encoding EPSPS)has been transformed into tobacco(Nicotiana tabacum)to develop novel plants with higher tolerance to glyphosate.Results from Southern and Western blotting analyses indicated that the target genes were integrated into tobacco chromosomes and expressed effectively at the protein level.Glyphosate tolerance was compared among transgenic tobacco plants containing gat,G2-aroA,or both genes.Plants containing both gat and G2-aroA genes were the most glyphosate-tolerant.This study has shown that a combination of different strategies may result in higher tolerance in transgenic crops,providing a new approach for development of glyphosate-tolerant crops. | Baoqing Dun Xujing Wang Wei Lu Ming Chen Wei Zhang Shuzhen Ping Zhixing Wang Baoming Zhang Min Lin | 2014 | The Crop Journal2014，2，Z1： | 4 |
| 2 | Effects of microtopography on spatial point pattern of forest stands on the semi-arid Loess Plateau,China显示文摘Microtopography may affect the distribution of forests through its effect on rain redistribution and soil water distribution on the semi-arid Loess Plateau,China.In this study,we investigated the characteristics of microtopography on two shady slopes(slope A,5 hm2,uniform slope;slope B,5 hm2,microtopography slope) and surveyed the height,the diameter at breast height and the location(x,y coordinates) of all selected individual trees(Robinia pseudoacacia Linn.,Pyrus betulifolia Bunge,Populus hopeiensis Hu & Chow,Armeniaca sibirica Lam.,Populus simonii Carr.and Ulmus pumila Linn.) on slope A and slope B in the watersheds of Wuqi county,Shaanxi province.Subsequently,the effects of microtopography on the spatial pattern of forest stands were analyzed using Ripley's K(r) function.The results showed that:(1) The maximal aggregation radiuses of the tree species on the uniform slope(slope A) were larger than 40 m,whereas those of the tree species on the microtopography slope(slope B) were smaller than 30 m.(2) On slope B,the spatial association of R.pseudoacacia with P.betulifolia,A.sibirica,P.simonii and U.pumila varied from being strongly negative to positive at microtopography scales.The spatial association of Populus hopeiensis Hu & Chow with U.pumila also varied from being strongly negative to positive at microtopography scales.However,there was no spatial association between P.betulifolia and P.hopeiensis,P.betulifolia and A.sibirica,P.betulifolia and P.simonii,P.betulifolia and U.pumila,P.hopeiensis and A.sibirica,P.hopeiensis and P.simonii,A.sibirica and P.simonii,A.sibirica and U.pumila,and P.simonii and U.pumila.On slope A,the spatial association between tree species were strongly negative.The results suggest that microtopography may shape tree distribution patterns on the semi-arid Loess Plateau. | WeiJun ZHAO Yan ZHANG QingKe ZHU Wei QIN ShuZhen PENG Ping LI YanMin ZHAO Huan MA Yu WANG | 2015 | Journal of Arid Land2015，7，3： | 3 |
| 3 | Functional identification of gene cluster for the aniline metabolic pathway mediated by transposable element显示文摘A convenient and widely applicable method has been developed to clone aniline metabolic gene cluster in this study. Three positive recombinant plasmids pDA1, pDB2 and pDB11 were cloned from genomic library of aniline degradation strain AD9. The result of aniline dioxygenase (AD) activity and catechol 2,3-oxygenase (C23O) activity assay showed that pDA1 and pDB11 contain aniline dioxy-genase genes and catechol 2,3-dioxygenase genes, respectively. The sequence analysis of the total 24.7-kb region revealed that this region contains 25 ORFs, of which 17 genes involve metabolism of aniline. In the gene cluster, the first five genes (tadQTA1A2B) and the subsequent gene (tadR1) were pre-dicted to encode a multi-component aniline dioxygenase and a LysR-type regulator, respectively, while the others (tadD1C1D2C2EFGIJKL) were expected to encode meta- cleavage pathway enzymes for catechol degradation. The gene cluster was surrounded by two IS1071 sequences. | LIANG Quanfeng CHEN Ming xu Yuquan ZHANG Wei PING Shuzhen LU Wei SONG Xianlong WANG Weiwei GENG Lizhao Takeo Masahiro LIN Min | 2005 | Chinese Science Bulletin2005，50，15： | 1 |
| 4 | Transmembrane structure and function of dctPQM encoding C4-dicarboxylate transport proteins from nitrogen-fixing P.stutzeri A1501显示文摘C4-dicarboxylate transport proteins of di-azotroph Pseudomonas stutzeri were encoded by dctPQM genes. Nucleotide sequence analysis indicated that dctP, dctQ, and dctM grouped together. Its nucleotide and amino acid sequence shared high homology with that of dctP gene en-coding periplasmic C4-dicarboxylate-binding protein and dctQM genes encoding C4-dicarboxylate transport proteins from the free-living nitrogen-fixing Aotobacter vinelandii. Structural analysis showed that DctP of P. stutzeri did not include membrane-spanning regions, and DctQ and DctM contained 5 and 12 transmembrane segments, respectively. The fragment containing the complete dctPQM genes was cloned into the Tn5 transposon region of suicide mobiliza-tion plasmid pSZ21. The resultant plasmid was named pSZY6. By triparental mating, Tn5 transposon carrying the dctPQM genes inserted into the genome of the wild type strain A1501, randomly. The recombinant strain A-142 which harboured an extra copy of dctPQM genes was con-structed and identified by PCR amplification of npt II gene. When A-142 was grown in minimal medium with different concentrations (20, 10 and 5 mmol/L) of C4-dicarboxylates succinate, malate, or fumarate as the sole carbon source, the rate of nitrogen fixation assayed by acetylene reduction was significantly higher than that of the wild-type strain A1501. This result was established that an extra copy of dctPQM genes could increase the activity of nitrogen fixation of P. stutzeri strain A1501. | YAN Chunling LIN Min WAN Yusong HOU Shengqiang PING Shuzhen CHEN Ming ZHANG Baoming C. Elmerich | 2003 | Chinese Science Bulletin2003，48，17： | 1 |
| 5 | A single residue mutation of 5-enoylpyruvylshikimate-3-phosphate synthase in Pseudomonas stutzeri enhances resistance to the herbicide glyphosate显示文摘 | Aimin Liang Jiying Sha Wei Lu Ming Chen Liang Li Dan Jin Yongliang Yan Jin Wang Shuzhen Ping Wei Zhang Yiding Wang Min Lin | | 0，2008，： | 1 |
| 6 | Identification of a New Gene Encoding EPSPS with High Glyphosate Resistance from the Metagenomic Library显示文摘 | Dan Jin Wei Lu Shuzhen Ping Wei Zhang Jian Chen Baoqing Dun Ruiqiang Ma Zhonglin Zhao Jiying Sha Liang Li Zhirong Yang Ming Chen Min Lin | | 0，2007，： | 1 |
| 7 | Multivariate and geostatistical analyses of the spatial distribution and origin of heavy metals in the agricultural soils in Shunyi, Beijing, China显示文摘 | Anxiang Lu Jihua Wang Xiangyang Qin Kaiyi Wang Ping Han Shuzhen Zhang | 2012 | Science of the Total Environment2012，，： | 1 |
| 8 | Characterization of Halomonas Varabilis Strain HTG7 Conferring Glyphosate Resistance显示文摘Bcterial strain HTG7 is isolated from extremely glyphosate-polluted soil. It is identified as Halomonas Varabilis. It can tolerate in 500 m mol/L glyphosate concentration. Physiological characterization of strain HTG7 shows that the optimum pH and temperature are 7.0 and 30℃, respectively. It grows well in the NaCl concentrations ranging from 0% to 10%. A plasmid pACYC184 carrying a 3.5 kb DNA fragment, which confers increased glyphosate tolerance, is cloned. The DNA fragment is able to complement with an E.coli auxotrophic aroA mutant. | 刘柱 Liang Aimin Ping Shuzhen Zhang Wei Chen Ming Yang Zhirong Lin Min | 2004 | High Technology Letters2004，10，1： | 0 |
| 9 | Analysis of hydroxylated polybrominated diphenyl ethers in plant samples using ultra performance liquid chromatography-mass spectrometry显示文摘A method was developed for the analysis of hydroxylated brominated diphenyl ethers (OH-PBDEs) in plant samples using an ultra performance liquid chromatography-triple quadrupole mass spectrometer (UPLC-ESI-MS/MS) in negative mode. Plant samples were extracted and cleaned up through florisil column, resolved on a 100 mm C18 column with linear gradient elution and detected by mass spectrometry in multiple reaction monitoring (MRM) mode. The method provided good recoveries rang- ing from 68.2% to 94.6%, relative standard deviation (RSD) in the range of 3.2% - 9.1%, and limits of quantification (LOQ) defined as the signal-to-noise ratio of 10 of 0.3-2.1 ng/g. It allowed a fast separation and sensitive quantification of the isomers and homologues of seven OH-PBDE congeners 2′-OH-BDE-3, 3′-OH-BDE-7, 4′-OH-BDE-17, 3′-OH-BDE-28, 3-OH-BDE-47, 5-OH-BDE-47 and 6-OH-BDE-47. The method was successfully applied to identify and quantify the formation of hydroxylat- ed metabolites in alfalfa exposed to BDE-209. Five OH-PBDEs were detected in plant tissues, and more congeners were found in roots than in shoots. To our knowledge, this work represents the first attempt to validate UPLC-MS/MS method to quantify OH-PBDEs in plant samples without derivatization. | WANG Sen WU Tong HUANG HongLin PING Hua LU AnXiang ZHANG ShuZhen | 2011 | Science China Chemistry2011，54，11： | 0 |
| 10 | Reconstruction of enzymatic activity from split genes encoding glyphosate-tolerant EPSPS protein of Psedomonas fluorescens G2 strain by intein mediated protein complementation显示文摘A mutagenesis library was constructed using GPS-LS system to insert a random 5 aa into the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) encoded by aroA gene. Active EPSPS pro- teins were identified by the ability to rescue growth of aroA-deleted mutant ER2799 on M9 minimal media. 12 unique sites, which can tolerate a 5-aa insertion, were identified. In all of the 12 sites, only F295/T296 site was found to split the G2-EPSPS properly by co-transformation of plasmids into E. coli ER2799. The G2-EPSPS gene was then divided into N-termi- nal and C-terminal from F295/T296 site which were fused to the N-terminal and C-terminal of Ssp.DnaE intein, respectively, creating two plasmids pMEPS- N295IN and pKEPSc296Ic. Co-transformation of plasmids, pMEPSN295IN and pKEPSc296Ic, rescu- ed growth of ER2799 in M9 minimal media, indicating that the intein splicing domains were bringing the EPSPS fragments together to generate activity. Re- consituted activity of splitted G2-EPSPS enzyme was 4.48 U/mg. | DUN Baoqing LU Wei ZHANG Wei PING Shuzhen WANG Xujing CHEN Ming XU Yuquan JIN Dan WANG Jin ZHAO Zhonglin LIANG Aimin HOU Songna XU Ming-Qun LIN Min | 2006 | Chinese Science Bulletin2006，51，13： | 0 |
| 11 | Expression profile analysis of the oxygen response in the nitrogen-fixing Pseudomonas stutzeri A1501 by genome-wide DNA microarray显示文摘Pseudomonas stutzeri A1501, an associative nitrogen-fixing bacterium, was isolated from the rice paddy rhizosphere. This bacterium fixes nitrogen under microaerobic conditions. In this study, ge- nome-wide DNA microarrays were used to analyze the global transcription profile of A1501 under aerobic and microaerobic conditions. The expression of 135 genes was significantly altered by more than 2-fold in response to oxygen stress. Among these genes, 68 were down-regulated under aerobic conditions; these genes included those responsible for nitrogen fixation and denitrification. Sixty- seven genes were up-regulated under aerobic conditions; these genes included sodC, encoding a copper-zinc superoxide dismutase, PST2179, encoding an NAD(P)-dependent oxidoreductase, PST3584, encoding a 2OG-Fe(II) oxygenase, and PST3602, encoding an NAD(P)H-flavin oxidoreductase. Addi- tionally, seven genes involved in capsular polysaccharide and antigen oligosaccharide biosynthesis together with 17 genes encoding proteins of unknown function were up-regulated under aerobic con- ditions. The overall analysis suggests that the genes we identified are involved in the protection of the bacterium from oxygen, but the mechanisms of their action remain to be elucidated. | DOU YueTan YAN YongLiang PING ShuZhen LU Wei CHEN Ming ZHANG Wei WANG YiPing JIN Qi LIN Min | 2008 | Chinese Science Bulletin2008，53，8： | 0 |
| 12 | A Histological Study on Subacute Toxicity of 'Baikening' in Hy-Line Brown Roosters显示文摘[Objective]A histological study was conducted to investigate the toxicity of'Baikening'and the toxic effect of'Baikening'on roosters after longerterm administration at different dose levels.[Method]Thirty 21-day-old Hy-Line Brown roosters were administered with 0.5 and 1.0 ml of'Baikening'aqueous extract through drinking water once a day for continuous 14 d.Roosters in control group and experimental groups after drug administration for 14 d and drug withdrawal for 7 d were euthanized;livers,kidneys,thymuses,spleens and bursas of Fabricius were collected and weighed for comparative analysis.Various tissues were prepared into paraffin-embedded slices and stained by H.E.,to observe histological changes of various organs under an optical microscope.[Result]After drug administration for 14 d,all the roosters in experimental groups survived;there was no visible pathological change in various organs of Hy-Line Brown roosters in each group;organ weights of Hy-Line Brown roosters in various groups showed no significant difference;observation results of tissue slices indicated that there was no pathological change in fine structures,nuclei and cytoplasm of various organs of Hy-Line Brown roosters in different groups;various blood vessels in different organs also presented no change in blood circulation or other pathological changes.[Conclusion]'Baikening'aqueous extract(oral administration)has no toxic and side effect on roosters in the subacute toxicity experiment. | Qiumei SHI Ping SHEN Shuzhen DONG Zhongjun HU | 2013 | Agricultural Biotechnology2013，2，6： | 0 |
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