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		题名	作者	年代	出处	被引量
	1	Enzymatic activity characterization of SARS coronavirus 3C-like protease by fluorescence resonance energy transfer technique显示文摘Aim: To characterize enzymatic activity of severe acute respiratory syndrome (SARS) coronavirus (CoV) 3C-like protease (3CL^pro) and its four site-directedmutants. Methods: Based on the fluorescence resonance energy transfer (FRET)principle using 5-[(2'-aminoethyl)-amino] naphthelenesulfonic acid (EDANS) and4-[[4-(dimethylamino) phenyl] azo] benzoic acid (Dabcyl) as the energy transferpair, one fluorogenic substrate was designed for the evaluation of SARS-CoV3CL^pro proteolytic activity. Results: The kinetic parameters of the fluorogenicsubstrate have been determined as Kin=404μmol·L^-1, kcat=1.08min^-1, and kcat/Km=2.7mmol^-1·L·min^-1. SARS-CoV 3CL^pro showed substantial pH and temperature-triggered activity switches, and site-directed mutagenesis analysis of SARS-CoV3CL^pro revealed that substitutions of His^41, Cys^145, and His^163 resulted in complete loss of enzymatic activity, while replacement of Met^162 with Ala caused strongly increased activity. Conclusion: This present work has provided valuable information for understanding the catalytic mechanism of SARS-CoV 3CL^pro. This FRET-based assay might supply an ideal approach for the exploration SARS-CoV 3CL^pro putative inhibitors.	ShuaiCHEN Li-liCHEN Hai-binLUO TaoSUN,JingCHEN JingCHEN FeiYIE Jian-huaCAI Jing-kangSHEN XuSHEN Hua-liangJIANG	2005	Acta Pharmacologica Sinica2005，26，1：	3
	2	Online Word‐of‐Mouth (or Mouse): An Exploration of Its Antecedents and Consequences显示文摘	TaoSun SeounmiYoun GuohuaWu ManaKuntaraporn	2006	Journal of Computer‐Mediated Communication2006，，4：	3
	3	Combined Effects of Glycated Hemoglobin A<sub>1c</sub> and Blood Pressure on Carotid Artery Atherosclerosis in Nondiabetic Patients显示文摘	WenZhu TaoSun HaimingShi JianLi JunZhu WeilingQi XinpingLuo YongLi	2010	Clin Cardiol2010，，9：	1
	4	Transient stability of DFIG wind turbines at an external short‐circuit fault显示文摘	TaoSun ZheChen FredeBlaabjerg	2005	Wind Energ2005，，3：	1
	5	Serological prevalence of echinococcosis and risk factors for infection among children in rural communities of southern Ningxia, China显示文摘	Yu RongYang Philip S.Craig Dominique A.Vuitton Gail M.Williams TaoSun Tian XiLiu BelgeesBoufana PatrickGiraudoux JingTeng YanbinLi LingHuang WeiZhang Malcolm K.Jones Donald P.McManus	2008	Tropical Medicine & International Health2008，，8：	1
	6	Transient stability of DFIG wind turbines at an external short‐circuit fault显示文摘	TaoSun ZheChen FredeBlaabjerg	2005	Wind Energ2005，，3：	1
	7	Activation of astroglial group Ⅱ and Ⅲ metabotropic glutamate receptors protects midbrain neurons against LPS or MPP^+ -induced neurotoxicity显示文摘AIM: Activation of glial metabotropic glutamate receptors (mGluRs) may be proved to play a critical role for neuroprotection in neurodegenerative diseases. Excess glutamate induced-excitoxicity is implicated in the initiation or progression of the neurodegenerative process. Glutamate accumulation in the central nervous system mediated by inhibiting	Hong-HongYao FangWang FangZhou Li-FangHu TaoSun Jian-HuaDing GangHu	2004	中国药理通讯2004，21，2：	0

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