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| 1 | Honokiol induces apoptosis through p53-independent pathway in human colorectal cell line RKO显示文摘AIM: To investigate the signal pathway of honokiol-induced apoptosis on human colorectal carcinoma RKO cells and to evaluate whether p53 and p53-related genes were involved in honokiol-treated RKO cells.METHODS: Cell cycle distribution and subdiploid peak were analyzed with a flow cytometer and DNA fragment with electrophoresis on agarose gels. Transcriptional level of Bax, Bcl-2, Bid and Bcl-xl was accessed by RT-PCR.Western blotting was used to measure p53 protein expression and other factors related to apoptosis.Proliferation inhibition of two cell lines (RKO, SW480) with high expression of p53 and one cell line with p53 negative expression (LS180) was monitored by MTr assay.RESULTS: Honokiol induced RKO cell apoptosis in a dosedependent manner. The mRNA expression level and proteinlevel of Bid were up-regulated while that of Bcl-xl wasdown-regulated, but no changes in Bax and Bcl-2 were observed. Western blotting showed p53 expression had no remarkable changes in honokiol-induced RKO cell apoptosis. LS180 cells treated with honokiol exhibited apparent growth inhibition like RKO ceils and Sw480 ceils.CONCLUSION: Honokiol can induce RKO cells apoptosis through activating caspase cascade by p53-indepenent pathway. | TaoWang FeiChen ZheChen Yi-FengWut Xiao-LiXu ShuZheng XunHu | 2004 | World Journal of Gastroenterology2004，10，15： | 27 |
| 2 | RELATIONSHIP BETWEEN ENDOTHELIAL DYSFUNCTION AND SERUM HOMOCYSTEINE IN PATIENTS WITH CORONARY LESIONS显示文摘Objective To investigate the relationship between vascular endothelial dysfunction and serum homocysteine (HCY) level in patients with coronary lesions. Methods Serum HCY, serum nitric oxide (NO), plasma endothelin-1 (ET-1), and circulation endothelial cell (CEC) were measured in 76 patients who received coronary angiography. Fifty-four patients with a stenosis of 50% or more at least in one coronary atery were as coronary artery disease (CAD) group. Other 22 cases with no recognizable plaque and/or stenosis were as control group. HCY level was detected using an enzyme immunoassay kit. NO concentration was measured using a nitrate reductase kit. Radio-immunoassay was applied to analyse the ET-1 level, and CEC was measured by flow cytometry. Results The levels of HCY, ET-1, and CEC in patients with coronary lesions were significantly increased in comparison with control group (P < 0.01), while NO level in CAD group was significantly lower compared with that in control (P < 0.01). Using a multivariate stepwise regression analysis, HCY level had a positive correlation with ET-1 level (r = 0.420, P < 0.05) and CECs number (r = 0.423, P < 0.05); and had a negative correlation with NO/ET-1 (r = -0.403, P < 0.05). But there was no significant correlation between HCY and NO levels. Conclusions HCY might lead to endothelial cell injury, which would provide a plausible mechanism for the relationship between hyperhomocysteinemia and development of coronary artery disease. HCY can be considered as a predictor for preli-minary or active coronary lesion. | ZheChen Chun-shengLi JianZhang Bao-senPang Cheng-qingXia Xi-fengLiu | 2005 | Chinese Medical Sciences Journal2005，20，1： | 10 |
| 3 | Engineering tailorable TiO_(2) nanotubes for NIR-controlled drug delivery显示文摘Infectious diseases caused by bacteria are a global threat to the human health. Here, we propose a solvent “irrigation” technique to endow TiO_(2) nanotubes (NTs) to precisely modify with functional nanomaterials, and apply them in constructing a near-infrared (NIR) light controlled drug-delivery system for rapid necrosis of bacteria. In this design, the NIR stimuli-responsive functional shell is located on the external tube wall of TiO_(2) NT;the internal tube wall offers sufficient binding sites for drug loading. Using kanamycin as a model drug, we demonstrate that the reactive oxygen species generated in photocatalysis not only controllably release the loaded drug by scissoring the linked chains, but also effectively compromise bacteria membrane integrity by damaging the cell wall. Benefiting from the damages, antibiotics rapidly enter the bacteria and reach ≥99.9% reduction in Escherichia coli colony within only 2 h. Importantly, such a covalently conjugation-based delivery system can efficiently relieve radical-induced inflammation and cytotoxicity. This study provides an innovative design strategy for engineering delivery systems with tailorable components, enduring stimuli-response by multiple triggers. | Yue Xu Chenxi Zhao Xi Zhang Jingwen Xu Lingling Yang Zhechen Zhang Zhida Gao Yan-Yan Song | 2021 | Nano Research2021，14，11： | 1 |
| 4 | A Combinative Assembly Strategy Inspired Reversibly Borate-Bridged Polymeric Micelles for Lesion-Specific Rapid Release of Anti-Coccidial Drugs显示文摘Stimuli-triggered drug delivery systems hold vast promise in local infection treatment for the site-specific targeting and shuttling of drugs.Herein,chitosan conjugates(SPCS)installed with sialic acid(SA)and phenylboronic acid(PBA)were synthesized,of which SA served as targeting ligand for coccidium and reversible-binding bridge for PBA.The enhanced drug-loading capacity of SPCS micelles was attributed to a combination assembly from hydrophobicity-driving and reversible borate bridges.The drug-loaded SPCS micelles shared superior biostability in upper gastrointestinal tract.After reaching the lesions,the borate bridges were snipped by carbohydrates under a higher pH followed by accelerated drug release,while SA exposure on micellar surface facilitated drug cellular internalization to eliminate parasites inside.The drugmicelles revealed an enhanced anti-coccidial capacity with a higher index of 185.72 compared with commercial preparation.The dual-responsive combination of physicochemical assembly could provide an efficient strategy for the exploitation of stable,safe and flexible anti-infectious drug delivery systems. | Hao Cheng Huaqing Zhang Gujun Xu Jin Peng Zhen Wang Bo Sun Djamila Aouameur Zhechen Fan Wenxin Jiang Jianping Zhou Yang Ding | 2020 | Nano-Micro Letters2020，12，11： | 1 |
| 5 | Transient stability of DFIG wind turbines at an external short‐circuit fault显示文摘 | TaoSun ZheChen FredeBlaabjerg | 2005 | Wind Energ2005，，3： | 1 |
| 6 | Solid-phasepreparation of ultra-fine PA6 powde-r through pan-milling显示文摘 | ZheChen Changsheng LIU | 2001 | Polymer engineering and science2001，41，： | 1 |
| 7 | Transient stability of DFIG wind turbines at an external short‐circuit fault显示文摘 | TaoSun ZheChen FredeBlaabjerg | 2005 | Wind Energ2005，，3： | 1 |
| 8 | Theoretical study of partial oxidation of ethylene by vanadium trioxide cluster cation显示文摘Density functional theory (DFT) study of reaction between vanadium trioxide cluster cation (VO3+) and ethylene (C2H4) to yield VO2+ + CH3CHO (acetaldehyde) and VO2CH2+ + HCHO (formaldehyde) is carried out. Structures of all reactants, products, intermediates, and transition state in the reaction have been optimized and characterized. The results show unexpected barriers in the reaction due to the existence of a η2-O2 moiety in the ground state structure of VO3+. The initial reaction steps combining ethylene adsorption, C=C activation and O-O cleavage are proposed as rate limiting processes. Comparison of reactions of VO3+ + C2H4 with VO3 + C2H4 and VO2+ + C2H4 in the previous studies is made in detail. The results of this work may shed light on the understanding of C=C bond cleavage in related heterogeneous catalysis. | WANG ZheChen DING XunLei MA YanPing CAO Hai WU XiaoNan ZHAO YanXia HE ShengGui | 2009 | Chinese Science Bulletin2009，54，16： | 0 |
| 9 | Unveiling the spatial distribution and molecular mechanisms of terpenoid biosynthesis in Salvia miltiorrhiza and S. grandifolia using multi-omicsand DESI-MSI显示文摘Salvia miltiorrhiza and S.grandifolia are rich in diterpenoids and have therapeutic effects on cardiovascular diseases.In this study,the spatial distribution of diterpenoids in both species was analyzed by a combination of metabolomics and mass spectrometry imaging techniques.The results indicated that diterpenoids in S.miltiorrhiza were mainly abietane-type norditerpenoid quinones with a furan or dihydrofuran D-ring and were mainly distributed in the periderm of the roots,e.g.cryptotanshinone and tanshinone IIA.The compounds in S.grandifolia were mainly phenolic abietane-type tricyclic diterpenoids with six-or seven-membered C-rings,and were widely distributed in the periderm,phloem,and xylem of the roots,e.g.11-hydroxy-sugiol,11,20-dihydroxy-sugiol,and 11,20-dihydroxy-ferruginol.In addition,the leaves of S.grandifolia were rich in tanshinone biosynthesis precursors,such as 11-hydroxy-sugiol,while those of S.miltiorrhiza were rich in phenolic acids.Genes in the upstream pathway of tanshinone biosynthesis were highly expressed in the root of S.grandifolia,and genes in the downstream pathway were highly expressed in the root of S.miltiorrhiza.Here,we describe the specific tissue distributions and mechanisms of diterpenoids in two Salvia species,which will facilitate further investigations of the biosynthesis of diterpenoids in plant synthetic biology. | Jie Xia Ganggui Lou Lan Zhang Yanbo Huang Jian Yang Juan Guo Zhechen Qi Zhenhao Li Guoliang Zhang Shengchun Xu Xijiao Song Xiaodan Zhang Yukun Wei Zongsuo Liang Dongfeng Yang | 2023 | Horticulture Research2023，10，7： | 0 |
| 10 | Progress and perspective on rechargeable magnesium-ion batteries显示文摘Current electrochemical energy storage technology has evolved a variety of rechargeable battery systems.Recently,the resource shortage of raw materials in commercially available lithium-ion batteries has attracted widespread attention.The requirements to meet resourcefulness,sustainability,safety,and high energy density have motivated the development of rechargeable magnesium-ion batteries(RMBs).Although RMBs have made significant progress so far,there are still many obstacles to practical orientation.We systematically summarize the significant progress and the latest research on RMBs,including Mg^(2+)-conducting electrolytes,Mg^(2+)-storage cathodes,and Mg-based anodes.In this review,we mainly introduce the properties and features of various Mg^(2+)-conductive electrolytes,the mainstream cathode materials,and their respective Mg^(2+)-storage mechanisms,as well as the Mg metal(or alloy)anodes and the corresponding modification strategies.Finally,the future directions in various key components for RMBs are highlighted. | Guixin Wang Zhechen Wang Hucheng Shi Aobing Du Mingliang Sun Guanglei Cui | 2024 | Science China Chemistry2024，67，1： | 0 |
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