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| 1 | Cdx1和Cdx2基因表达异常与先天性结肠性肠炎的关系显示文摘目的 结肠炎是先天性巨结肠患儿常见的并发症,组织学研究表明巨结肠性肠炎患儿肠粘膜内的内分泌细胞减少和粘膜内分泌型 IgA不足。而Cdx1和Cdx2基因调控结肠粘膜上皮细胞的分化和增殖,本研究的目的是明确Cdx1和Cdx2基因表达与先天性巨结肠性肠炎之间的关系。方法 采用半定量RT-PCR反应和原位杂交方法,11例先天性巨结肠患儿(年龄1~30个月,4例合并结肠炎)的无神经节细胞段、移行段和扩张段的手术切除标本为病例组,12例年龄相符(1~30个月)的非巨结肠患儿的远端结肠标本为对照组,观察Cdx1和Cdx2基因在其中的表达情况。结果Cdx1和Cdx2基因在对比组和先天性巨结肠的结肠粘膜上皮细胞均有表达,但是此二基因在先天性巨结肠性肠炎患儿的标本中表达明显减弱。结论 本研究结果表明Cdx1和Cdx2基因表达减弱与先天性巨结肠性肠炎的病因密切相关。 | 李龙 Vincent Lui Mai Har Sham 谭广 | 2003 | 中华小儿外科杂志2003，24，5： | 9 |
| 2 | Role of CD56-expressing immature biliary epithelial cells in biliary atresia显示文摘AIM: To analyze the clinical and pathological parameters and expression of the neural cell adhesion molecule(CD56) in patients with biliary atresia(BA).METHODS: Established clinical laboratory markers of hepatic function, including enzyme activity, protein synthesis, and bilirubin metabolism, were evaluated in patients with BA and compared with those in patients with choledochal cysts and neonatal hepatitis. Pathological changes in tissue morphology and fibrosis were examined by histological and tissue collagen staining. Immunohistochemical staining for the biliary epithelial cell markers CD56 and CK19 together with the Notch signaling related molecules Notch1 and Notch2 was performed in the context of alterations in the structure of intrahepatic biliary ducts.RESULTS: Differences in some clinical laboratoryparameters among the three diseases examined were observed, but they did not correlate with the pathological classification of fibrosis in BA. Immunohistochemical staining showed the presence of CD56-positive immature bile ducts in most patients(74.5%) with BA but not in patients with choledochal cysts or neonatal hepatitis. The number of CD56-expressing cells correlated with disease severity, with more positive cells present in the later stages of liver damage(81.8% vs 18.2%). Furthermore, bile plugs were mainly found in CD56-positive immature biliary ducts. Notch signaling was a key regulatory pathway in biliary duct formation and played a role in tissue fibrosis. Notch1 was co-expressed in CD56-positive cells, whereas Notch2 was found exclusively in blood vessels in the portal area of patients with BA. CONCLUSION: The maturation of biliary epithelial cells and the expression of Notch may play a role in the pathogenesis of BA. | Rui-Zhong Zhang Jia-Kang Yu Jiao Peng Feng-Hua Wang Hai-Ying Liu Vincent CH Lui John M Nicholls Paul KH Tam Jonathan R Lamb Yan Chen Hui-Min Xia | 2016 | World Journal of Gastroenterology2016，22，8： | 8 |
| 3 | Downregulation of Hes1 expression in experimental biliary atresia and its effects on bile duct structure显示文摘AIM To analyze the expression and function of the notchsignaling target gene Hes1 in a rhesus rotavirusinduced mouse biliary atresia model. METHODS The morphologies of biliary epithelial cells in biliary atresia patients and in a mouse model were examined by immunohistochemical staining. Then, the differential expression of Notch signaling pathway-related molecules was investigated. Further, the effects of the si RNAmediated inhibition of Hes1 expression were examined using a biliary epithelial cell 3 D culture system.RESULTS Both immature(Ep CAM+) and mature(CK19+) biliary epithelial cells were detected in the livers of biliary atresia patients without a ductile structure and in the mouse model with a distorted bile duct structure. The hepatic expression of transcripts for most Notch signaling molecules were significantly reduced on day 7 but recovered to normal levels by day 14, except for the target molecule Hes1, which still exhibited lower m RNA and protein levels. Expression of the Hes1 transcriptional co-regulator, RBP-Jκ was also reduced. A 3 D gel culture system promoted the maturation of immature biliary epithelial cells, with increased expression of CK19+ cells and the formation of a duct-like structure. The administration of Hes1 si RNA blocked this process. As a result, the cells remained in an immature state, and no duct-like structure was observed.CONCLUSION Our data indicated that Hes1 might contribute to the maturation and the cellular structure organization of biliary epithelial cells, which provides new insight into understanding the pathology of biliary atresia. | Rui-Zhong Zhang Xin-Hao Zeng Ze-Feng Lin Ming-Fu Yan-Lu Tong Vincent CH Lui Paul KH Tam Jonathan R Lamb Hui-Min Xia Yan Chen | 2018 | World Journal of Gastroenterology2018，24，29： | 5 |
| 4 | Depletion of the IKBKAP ortholog in zebrafish leads to hirschsprung disease-like phenotype显示文摘AIM:To investigate the role of IKBKAP(inhibitor of kappa light polypeptide gene enhancer in B-cells,kinase complexassociated protein)in the development of enteric nervous system(ENS)and Hirschsprung disease(HSCR).METHODS:In this study,we injected a morpholino that blocked the translation of ikbkap protein to 1-cell stage zebrafish embryos.The phenotype in the ENS was analysed by antibody staining of the pan-neuronal marker Hu C/D followed by enteric neuron counting.The mean numbers of enteric neurons were compared between the morphant and the control.We also studied the expressions of ret and phox2bb,which are involved in ENS development,in the ikbkap morpholino injected embryos by quantitative reverse transcriptase polymerase chain reaction and compared them with the control.RESULTS:We observed aganglionosis(χ2,P<0.01)and a reduced number of enteric neurons(38.8±9.9 vs50.2±17.3,P<0.05)in the zebrafish embryos injected with ikbkap translation-blocking morpholino(morphant)when compared with the control embryos.Specificity of the morpholino was confirmed by similar results obtained using a second non-overlapping morpholino that blocked the translation of ikbkap.We further studied the morphant by analysing the expression levels of genes involved in ENS development such as ret,phox2bb and sox10,and found that phox2bb,the ortholog of human PHOX2B,was significantly down-regulated(0.51±0.15 vs 1.00±0,P<0.05).Although we also observed a reduction in theexpression of ret,the difference was not significant.CONCLUSION:Loss of IKBKAP contributed to HSCR as demonstrated by functional analysis in zebrafish embryos. | William Wai-Chun Cheng Clara Sze-Man Tang Hong-Sheng Gui Man-Ting So Vincent Chi-Hang Lui Paul Kwong-Hang Tam Maria-Mercè Garcia-Barcelo | 2015 | World Journal of Gastroenterology2015，21，7： | 2 |
| 5 | Current systematic reviews and meta-analyses of COVID-19显示文摘BACKGROUND Coronavirus disease 2019(COVID-19)has left a significant impact on the world's health,economic and political systems;as of November 20,2020,more than 57 million people have been infected worldwide,with over 1.3 million deaths.While the global spotlight is currently focused on combating this pandemic through means ranging from finding a treatment among existing therapeutic agents to inventing a vaccine that can aid in halting the further loss of life.AIM To collect all systematic reviews and meta-analyses published related to COVID-19 to better identify available evidence,highlight gaps in knowledge,and elucidate further meta-analyses and umbrella reviews that are yet to be performed.METHODS We explored studies based on systematic reviews and meta-analyses with the keyterms,including severe acute respiratory syndrome(SARS),SARS virus,coronavirus disease,COVID-19,and SARS coronavirus-2.The included studies were extracted from Embase,Medline,and Cochrane databases.The publication timeframe of included studies ranged between January 01,2020,to October 30,2020.Studies that were published in languages other than English were not considered for this systematic review.The finalized full-text articles are freely accessible in the public domain.RESULTS Searching Embase,Medline,and Cochrane databases resulted in 1906,669,and 19 results,respectively,that comprised 2594 studies.515 duplicates were subsequently removed,leaving 2079 studies.The inclusion criteria were systematic reviews or meta-analyses.860 results were excluded for being a review article,scope review,rapid review,panel review,or guideline that produced a total of 1219 studies.After screening articles were categorized,the included articles were put into main groups of clinical presentation,epi-demiology,screening and diagnosis,severity assessment,special populations,and treatment.Subsequently,there was a second subclassification into the following groups:gastrointestinal,cardiovascular,neurological,stroke,thrombosis,anosmia and dysgeusia,ocular manifestations,nephrology,cutaneous manifestations,D-dimer,lymphocyte,anticoagulation,antivirals,convalescent plasma,immunosuppressants,corticosteroids,hydroxychloroquine,renin-angiotensin-aldosterone system,technology,diabetes mellitus,obesity,pregnancy,children,mental health,smoking,cancer,and transplant.CONCLUSION Among the included articles,it is clear that further research is needed regarding treatment options and vaccines.With more studies,data will be less heterogeneous,and statistical analysis can be better applied to provide more robust clinical evidence.This study was not designed to give recommendations regarding the management of COVID-19. | Mahmoud Nassar Nso Nso Mostafa Alfishawy Anastasia Novikov Salim Yaghi Luis Medina Bahtiyar Toz Sofia Lakhdar Zarwa Idrees Yungmin Kim Dawa Ongyal Gurung Raheel S Siddiqui David Zheng Mariam Agladze Vikram Sumbly Jasmine Sandhu Francisco Cuevas Castillo Nadya Chowdhury Ravali Kondaveeti Sakil Bhuiyan Laura Guzman Perez Riki Ranat Carlos Gonzalez Harangad Bhangoo John Williams Alaa Eldin Osman Joyce Kong Jonathan Ariyaratnam Mahmoud Mohamed Ismail Omran Mariely Lopez Akwe Nyabera Ian Landry Saba Iqbal Anoosh Zafar Gondal Sameen Hassan Ahmed Daoud Bahaaeldin Baraka Theo Trandafirescu Vincent Rizzo | 2021 | World Journal of Virology2021，10，4： | 2 |
| 6 | High Incidence of Mortality and Recurrent Bleeding in Patients With Helicobacter pylori –Negative Idiopathic Bleeding Ulcers显示文摘 | Grace Lai–Hung Wong Vincent Wai–Sun Wong Yawen Chan Jessica Yuet–Ling Ching Kim Au Aric Josun Hui Larry Hin Lai Dorothy Kai–Lai Chow Danny Ka–Fai Siu Yan–Ni Lui Justin Che–Yuen Wu Ka–Fai To Lawrence Cheung–Tsui Hung Henry Lik–Yuen Chan Joseph Jao–Yiu Sung | 2009 | Gastroenterology2009，，2： | 2 |
| 7 | Valued outranking relations in ELEC- TRE providing manageable disaggregation procedures 显示文摘 | Vincent Mousseau Luis Dias | 2004 | European Journal of Operational Research2004，，156： | 1 |
| 8 | Transfer of quinolone resistance gene qnrA1 to Escherichia coli through a 50 kb conjugative plasmid resulting from the splitting of a 300 kb plasmid显示文摘 | Ulises Garza-Ramos Humberto Barrios Maria Jose Hernandez-Vargas Teresa Rojas-Moreno Fernando Reyna-Flores Perla Tinoco Victor Othon Laurent Poirel Patrice Nordmann Vincent Cattoir Guillermo Ruiz-Palacios Jose Luis Fernandez Rosa Isela Santamaria Patricia | 2012 | Journal of Antimicrobial Chemotherapy2012，，7： | 1 |
| 9 | Valued outran- king relations in ELECTRE providing manageable disag- gregation procedures 显示文摘 | VINCENT MOUSSEAU LUIS DIAS | 2004 | European Journal of Operation- al Research2004，152，6： | 1 |
| 10 | The SSN ontology of the W3C semantic sensor network incubator group显示文摘 | Michael Compton Payam Barnaghi Luis Bermudez Raúl García-Castro Oscar Corcho Simon Cox John Graybeal Manfred Hauswirth Cory Henson Arthur Herzog Vincent Huang Krzysztof Janowicz W. David Kelsey Danh Le Phuoc Laurent Lefort Myriam Leggieri Holger Neuhaus A | 2012 | Web Semantics: Science Services and Agents on the World Wide Web2012，，： | 1 |
| 11 | The SSN ontology of the W3C semantic sensor network incubator group显示文摘 | Michael Compton Payam Barnaghi Luis Bermudez Raúl García-Castro Oscar Corcho Simon Cox John Graybeal Manfred Hauswirth Cory Henson Arthur Herzog Vincent Huang Krzysztof Janowicz W. David Kelsey Danh Le Phuoc Laurent Lefort Myriam Leggieri Holger Neuhaus A | 2012 | Web Semantics: Science, Services and Agents on the World Wide Web2012，，： | 1 |
| 12 | Transcriptional regulation of RET by Nkx2-1, Phox2b, Sox10, and Pax3显示文摘 | Thomas Y.Y. Leon Elly S.W. Ngan Hiu-Ching Poon Man-Ting So Vincent C.H. Lui Paul K.H. Tam Maria Mercedes Garcia-Barcelo | 2009 | Journal of Pediatric Surgery2009，，10： | 1 |
| 13 | CXCR4/CXCL12 Mediate Autocrine Cell- Cycle Progression in NF1-Associated Malignant Peripheral Nerve Sheath Tumors显示文摘 | Wei Mo Jian Chen Amish Patel Liang Zhang Vincent Chau Yanjiao Li Woosung Cho Kyun Lim Jing Xu Alexander J. Lazar Chad J. Creighton Svetlana Bolshakov Renée M. McKay Dina Lev Lu Q. Le Luis F. Parada | 2013 | Cell2013，，： | 1 |
| 14 | Valued outranking relations in ELECTRE providing manageable disaggregation procedures 显示文摘 | VINCENT MOUSSEAU LUIS DIAS | 2004 | European Journal of Operational Research2004，，： | 1 |
| 15 | Liver kidney crosstalk:Hepatorenal syndrome显示文摘The dying liver causes the suffocation of the kidneys,which is a simplified way of describing the pathophysiology of hepatorenal syndrome(HRS).HRS is characterized by reversible functional renal impairment due to reduced blood supply and glomerular filtration rate,secondary to increased vasodilators.Over the years,HRS has gained much attention and focus among hepatologists and nephrologists.HRS is a diagnosis of exclusion,and in some cases,it carries a poor prognosis.Different classifications have emerged to better understand,diagnose,and promptly treat this condition.This targeted review aims to provide substantial insight into the epidemiology,pathophysiology,diagnosis,and management of HRS,shed light on the various milestones of this condition,and add to our current understanding. | Mahmoud Nassar Nso Nso Luis Medina Victoria Ghernautan Anastasia Novikov Alli El-Ijla Karim M Soliman Yungmin Kim Mostafa Alfishawy Vincent Rizzo Ahmed Daoud | 2021 | World Journal of Hepatology2021，13，9： | 0 |
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