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| 1 | A Novel Coronavirus Genome Identified in a Cluster of Pneumonia Cases--Wuhan,China 2019−2020显示文摘Emerging and re-emerging pathogens are great challenges to the public health(1).A cluster of pneumonia cases with an unknown cause occurred in Wuhan starting on December 21,2019.As of January 20,2020,a total of 201 cases of pneumonia in China have been confirmed.A team of professionals from the National Health Commission and China CDC conducted epidemiological and etiological investigations.On January 3,2020,the first complete genome of the novelβgenus coronaviruses(2019-nCoVs)was identified in samples of bronchoalveolar lavage fluid(BALF)from a patient from Wuhan by scientists of the National Institute of Viral Disease Control and Prevention(IVDC)through a combination of Sanger sequencing,Illumina sequencing,and nanopore sequencing.Three distinct strains have been identified,the virus has been designated as 2019-nCoV,and the disease has been subsequently named novel coronavirus-infected pneumonia(NCIP). | Wenjie Tan Xiang Zhao Xuejun Ma Wenling Wang Peihua Niu Wenbo Xu George F.Gao Guizhen Wu | 2020 | China CDC weekly2020，2，4： | 95 |
| 2 | GDNF-Enhanced Axonal Regeneration and Myelination Following Spinal Cord Injury is Mediated by Primary Effects on Neurons显示文摘我们先前研究表明胶质细胞源性神经营养因子(GDNF)联合施万细胞移植能促进脊髓损伤后轴突再生和髓鞘形成。然而,GDNF介导这一过程的细胞靶点尚不清楚。在此,我们报道了GDNF可增加在体再生轴突的数目和直径,并促进体外背根神经节神经元的轴突向外生长,提示GDNF对神经元有直接作用。在施万细胞-背根神经节神经元共培养下,GDNF显著增加施万细胞生成的髓鞘数目;GDNF处理对孤立培养的施万细胞增殖无作用,但可促进已与神经轴突有突触联系的施万细胞增殖;GDNF可增加孤立施万细胞中分子量为140kDa的神经细胞黏附分子(NCAM)的表达,但对黏附分子L1表达或神经营养因子NGF、NT3及BDNF分泌没有影响。总之,这些结果支持假设:GDNF提高轴突再生和施万细胞髓鞘形成主要是通过GDNF对神经元的直接作用介导的,并且提示GDNF联合施万细胞移植可能是促进脊髓损伤后轴突再生和髓鞘形成的有效策略之一。 | LIQUN ZHANG ZHENGWEN MA GEORGE M. SMITH XUEJUN WEN YELENA PRESSMAN PATRICK M. WOOD AND XIAO-MING XU | 2009 | 神经损伤与功能重建2009，4，4： | 25 |
| 3 | Research on heart sound identification technology显示文摘In this article,both the basic principles and the experimental methods of identity recognition technology based on heart sounds are addressed.First,the characteristics of heart sounds and the feasibility of heart sounds as a biometric were analyzed.A synthetic model of heart sounds was then developed based on a family of wavelets;Finally,the characteristic parameters of heart sounds were extracted by using the heart sounds linear band frequency cepstra(HS-LBFC)with a specified configuration,and the similarity distance was adopted for heart sound identification.To highlight the difference in two heart sound signals between the time and frequency domains,a construction method of the heart sound wavelet,a calculation method of the parameters of a synthetic model,selection of the characteristic parameters of heart sounds and the corresponding data processing technology were examined.The experimental results showed that the proposed method exhibited excellent performance and practicability. | CHENG XieFeng MA Yong LIU Chen ZHANG XueJun GUO YuFeng | 2012 | Science China(Information Sciences)2012，55，2： | 20 |
| 4 | First light of the 1.8-m solar telescope–CLST显示文摘Large-aperture solar telescopes play an important role in solar observations and research,and require high temporal and spatial resolution[1].To solve some fundamental problems such as the solar dynamo,coronal heating,and the triggering of major solar eruptions,the spatial resolution for solar-atmosphere observation should reach at least 0.1 arcsec[2]. | ChangHui Rao NaiTing Gu XueJun Rao Cheng Li LanQiang Zhang JinLong Huang Lin Kong Ming Zhang YunTao Cheng Yi Pu Hua Bao YouMing Guo YangYi Liu JinSheng Yang LiBo Zhong ChangJun Wang Kai Fang XiaoJun Zhang DongHong Chen Cheng Wang XinLong Fan ZhiWu Yan KeLe Chen XiYa Wei Lei Zhu Hong Liu YongJian Wan Hao Xian WenLi Ma | 2020 | Science China(Physics,Mechanics & Astronomy)2020，63，10： | 6 |
| 5 | Development of an innovative one-step nested PCR strategy for virus detection using the LNA technique显示文摘Dear Editor,Polymerase chain reaction(PCR)is a molecular diagnostic technique that has been widely used for diagnosing viral diseases.Nested PCR(N-PCR)is a variation on the standard PCR technique that involves two amplification steps,yielding greater sensitivity and specificity.But there are two main disadvantages:the protocol is more complex than conventional PCR,and the risk of cross-contamination is relatively high.One-step nested PCR(OSN-PCR)enables conventional N-PCR to be performed as a closed reaction in a single tube that contains both outer and inner primers.This strategy makes OSN-PCR more rapid than N-PCR,lowers the probability of cross-contamination,and requires a smaller amount of reagents. | Ji Wang Li Zhao Zhihua Sun Guixia Li Peihua Niu DANDi Li Lianjun Wang Yi Zhang Zhishan Feng Xuejun Ma | 2019 | Science China(Life Sciences)2019，62，3： | 4 |
| 6 | Physiological levels of ATP negatively regulate proteasome function显示文摘由 ubiquitin-proteasome 系统的细胞内部的蛋白质降级是 ATP 依赖者,和最佳的 ATP 集中激活 proteasome 在 vitro 的功能是 ~ 100 渭 M。细胞内部的 ATP 层次在在这个范围以内的水平通常在低 millimolar 范围,而是 ATP 被显示在 vitro 禁止 proteasome peptidase 活动。这里,我们报导支持在生理的层次的细胞内部的 ATP 双向地调整的一个假设的新证据 26S proteasome 在房间的解朊的功能。首先,我们证实 ATP 在 vitro 在 26S proteasome 上施加了双向规定,与最佳的 ATP 集中(在 50 和 100 渭 M 之间) 刺激 proteasome 像糜蛋白酶的活动。第二,我们发现操作细胞内部的 ATP 层次也在有教养的房间在 proteasome 特定的蛋白质底层的层次导致了双向变化。最后,测量增加提高的细胞内部的 ATP,当减少的细胞内部的 ATP 稀释了导致房间死亡的 proteasome 抑制的能力时。这些数据强烈建议在生理的集中范围以内的内长的 ATP 能在 proteasome 活动施加否定影响,允许房间到很快,在应力下面的 ATP 减小上的 upregulate proteasome 活动调节。 | Hongbiao Huang Xiaoyan Zhang Shujue Li Ningning Liu Wen Elan Emily McDowell Ping Zhou Canguo Zhao Haiping Guo Change Zhang Changshan Yang Guangmei Wen Xiaoxian Dong Li Lu Ningfang Ma Weihua Dong Q Ping Dou Xuejun Wang Jinbao Liu | 2010 | Cell Research2010，20，12： | 4 |
| 7 | Identification of Histoplasma causing an unexplained disease cluster in Matthews Ridge, Guyana显示文摘Here,we report the identification of Histoplasma causing an unexplained disease cluster in Matthews Ridge,Guyana.In March 2019,14 employees of Chongqing Bosai Mining Company,China,working in a manganese mining of Guyana,had unexplained fever,and two of them died.We obtained lung and brain tissues as well as the blood samples from the two deceased cases(patient No.1 and 2),and bronchoscopy lavages and cerebrospinal fluid samples from one severe case(patient No.3),respectively.All samples were tested by pathological examination,high-throughput sequencing,and real-time PCR.Pathological detection showed the presence of spore-like structures in the lung tissue of patient No.1,indicating a fungal infection in this patient.Nanopore sequencing identified the existing of H.capsulatum in the lung tissue sample within 13 h.Next-generation sequencing identified specific fragments of H.capsulatum in all of the samples tested(lung,brain and blood serum from the deceased cases,and plasma from the severe case).Real-time PCR assays did not reveal any viral infection related to transmission from bat feces.We conclude that H.capsulatum was the causative pathogen of this disease cluster based on epidemiologic,clinical,pathological and nucleic acid evidence. | Ji Wang Weimin Zhou Hua Ling Xiaoping Dong Yi Zhang Jiandong Li Yong Zhang Jingdong Song William J.Liu Yang Li Ruiqing Zhang Wei Zhen Kun Cai Shuangli Zhu Dongyan Wang Jinbo Xiao Yigang Tong Wenli Liu Lihua Song Wei Wu Yang Liu Xiang Zhao Ruihuan Wang Sheng Ye Jing Wang Roujian Lu Baoying Huang Fei Ye Wenwen Lei Rongbao Gao Qi Shi Cao Chen Jun Han Wenbo Xu George F.Gao Xuejun Ma Guizhen Wu | 2019 | Biosafety and Health2019，1，3： | 3 |
| 8 | PM_(2.5)and water-soluble inorganic ion concentrations decreased faster in urban than rural areas in China显示文摘We investigated variations of PM_(2.5)and water-soluble inorganic ions chemical characteristics at nine urban and rural sites in China using ground-based observations.From 2015 to 2019,mean PM_(2.5)concentration across all sites decreased by 41.9μg/m~3with a decline of 46%at urban sites and 28%at rural sites,where secondary inorganic aerosol(SIAs)contributed to 21%(urban sites)and 17%(rural sites)of the decreased PM_(2.5).SIAs concentrations underwent a decline at urban locations,while sulfate(SO_(4)^(2–)),nitrate(NO_(3)^(–)),and ammonium(NH_(4)^(+))decreased by 49.5%,31.3%and 31.6%,respectively.However,only SO_(4)^(2–)decreased at rural sites,NO_(3)^(–)increased by 21%and NH_(4)^(+)decreased slightly.Those changes contributed to an overall SIAs increase in 2019.Higher molar ratios of NO_(3)^(–)to SO_(4)^(2–)and NH_(4)^(+)to SO_(4)^(2–)were observed at urban sites than rural sites,being highest in the heavily polluted days.Mean molar ratios of NH_(3)/NH_xwere higher in 2019 than 2015 at both urban and rural sites,implying increasing NH_xremained as free NH_(3).Our observations indicated a slower transition from sulfate-driven to nitrate-driven aerosol pollution and less efficient control of NO_(x)than SO_(2)related aerosol formation in rural regions than urban regions.Moreover,the common factor at urban and rural sites appears to be a combination of lower SO_(4)^(2–)levels and an increasing fraction of NO_(3)^(–)to PM_(2.5)under NH_(4)^(+)-rich conditions.Our findings imply that synchronous reduction in NO_(x)and NH_(3)emissions especially rural areas would be effective to mitigate NO_(3)^(–)-driven aerosol pollution. | Yangyang Zhang Aohan Tang Chen Wang Xin Ma Yunzhe Li Wen Xu Xiaoping Xia Aihua Zheng Wenqing Li Zengguo Fang Xiufen Zhao Xianlong Peng Yuping Zhang Jian Han Lijuan Zhang Jeffrey L.Collett Jr Xuejun Liu | 2022 | Journal of Environmental Sciences2022，34，12： | 2 |
| 9 | Reliability analysis based on a novel density estimation method for structures with correlations显示文摘Estimating the Probability Density Function(PDF) of the performance function is a direct way for structural reliability analysis,and the failure probability can be easily obtained by integration in the failure domain.However,efficiently estimating the PDF is still an urgent problem to be solved.The existing fractional moment based maximum entropy has provided a very advanced method for the PDF estimation,whereas the main shortcoming is that it limits the application of the reliability analysis method only to structures with independent inputs.While in fact,structures with correlated inputs always exist in engineering,thus this paper improves the maximum entropy method,and applies the Unscented Transformation(UT) technique to compute the fractional moments of the performance function for structures with correlations,which is a very efficient moment estimation method for models with any inputs.The proposed method can precisely estimate the probability distributions of performance functions for structures with correlations.Besides,the number of function evaluations of the proposed method in reliability analysis,which is determined by UT,is really small.Several examples are employed to illustrate the accuracy and advantages of the proposed method. | Baoyu LI Leigang ZHANG Xuejun ZHU Xiongqing YU Xiaodong MA | 2017 | Chinese Journal of Aeronautics2017，30，3： | 2 |
| 10 | Contrasting effects of nitrogen addition on litter decomposition in forests and grasslands in China显示文摘Nitrogen(N)addition has profound impacts on litter-mediated nutrient cycling.Numerous studies have reported different effects of N addition on litter decomposition,exhibiting positive,negative,or neutral effects.Previous meta-analysis of litter decomposition under N addition was mainly based on a small number of samples to allow comparisons among ecosystem types.This study presents the results of a meta-analysis incorporating data from 53 published studies(including 617 observations)across forests,grasslands,wetlands,and croplands in China,to investigate how environmental and experimental factors impact the effects of N addition on litter decomposition.Averaged across all of the studies,N addition significantly slows litter decomposition by 7.02%.Considering ecosystem types,N addition significantly accelerates litter decomposition by 3.70%and 11.22%in grasslands and wetlands,respectively,clearly inhibits litter decomposition by 14.53%in forests,and has no significant effects on litter decomposition in croplands.Regarding the accelerated litter decomposition rate in grasslands due to N addition,litter decomposition rate increases slightly with increasing rates of N addition.However,N addition slows litter decomposition in forests,but litter decomposition is at a significantly increasing rate with increasing amounts of N addition.The responses of litter decomposition to N addition are also influenced by the forms of N addition,experiential duration of N addition,humidity index,litter quality,and soil pH.In summary,N addition alters litter decomposition rate,but the direction and magnitude of the response are affected by the forms of N addition,the rate of N addition,ambient N deposition,experimental duration,and climate factors.Our study highlights the contrasting effects of N addition on litter decomposition in forests and grasslands.This finding could be used in biogeochemical models to better evaluate ecosystem carbon cycling under increasing N deposition due to the differential responses of litter decomposition to N addition rates and ecosystem types. | SU Yuan MA Xiaofei GONG Yanming LI Kaihui HAN Wenxuan LIU Xuejun | 2021 | Journal of Arid Land2021，13，7： | 2 |
| 11 | Cadmium pollution from phosphate fertilizers in arable soils and crops:an overview显示文摘The application of mineral and organic phosphorus fertilizers to arable land has greatly increased crop yield to meet the world food demand.On the other hand,impurities in these fertilizers,such as heavy metals,are being added to agricultural soils,resulting both from the raw materials themselves and the processes used to obtain the final product.Cadmium,a non-essential and toxic heavy metal,has been found in relatively high amounts in common P fertilizers obtained from sediments.This metal poses a high risk for soil fertility,crop cultivation,and plants in general.Furthermore,human health might be compromised by the cadmium concentrations in agricultural and livestock products,due to the bioaccumulation effect in the food web.The accumulation in the different matrixes is the result of the high mobility and flexible availability of this harmful metal.This review summarizes risks to human health,the factors influencing cadmium movement in soils and crop uptake,as well as common plant responses to its toxicity.In addition,it summarizes cadmium balances in soils,trends,long-term experiments,and further studies.Cadmium inputs and outputs in arable soil,together with their calculated concentrations,are compared between two different regions:the European countries(in particular Germany)and China.The comparison appears useful because of the different proportions in the inputs and outputs of cadmium,and the diverse geographical,environmental and social factors.Moreover,these variables and their influences on cadmium contamination improve the understanding of the pollution from phosphate fertilizers and will help to establish future mitigation policies. | Andrea Giovanna NINO-SAVALA Zhong ZHUANG Xin MA Andreas FANGMEIER Huafen LI Aohan TANG Xuejun LIU | 2019 | Frontiers of Agricultural Science and Engineering2019，6，4： | 2 |
| 12 | Genomic Surveillance for SARS-CoV-2 Variants of Concern from Imported COVID-19 Cases-the Mainland of China,2021显示文摘Introduction:After the epidemic in Wuhan City was brought under control in 2020,local outbreaks of coronavirus disease 2019(COVID-19)in the mainland of China were mainly due to imported COVID-19 cases.The ongoing evolution of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has continued to generate new variants.Some have been designated as variants of concern(VOCs)by the World Health Organization(WHO).To better assess the role of imported SARS-CoV-2 surveillance and the prevalence of VOCs in 2021,the genomic surveillance data of SARS-CoV-2 from imported COVID-19 cases of 2021 in the mainland of China were analyzed.Methods:The analyses included the number of sequence submissions,time of sequence deposition,and time of detection of the VOCs in order to determine the timeliness and sensitivity of the surveillance.The proportions of VOCs were analyzed and compared with data from the Global Initiative of Sharing All Influenza Data(GISAID).Results:A total of 3,355 sequences of imported cases were submitted from 29 provincial-level administrative divisions,with differences in the number of sequence submissions and median time of sequence deposition.A total of 2,388 sequences with more than 90%genomic coverage were used for lineage analysis.The epidemic trend from Alpha to Delta to Omicron in imported cases was consistent with that in the GISAID.In addition,VOCs from imported cases were usually identified after WHO designation and before causing local outbreaks.Conclusions:The global distribution of SARSCoV-2 VOCs changed rapidly in 2021.Robust genomic surveillance of the imported SARS-CoV-2 in the mainland of China is of great significance.Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the etiological agent of coronavirus disease 2019(COVID-19),is constantly mutating under the different circumstances of global transmission(1).The emerging SARS-CoV-2 variants may have potential adverse impacts on epidemic traits and severity.To some extent,it is also capable of escaping natural and vaccine-induced immunity(2–3).Some of them were designated as variants of concern(VOCs)by the World Health Organization(WHO)(4).Therefore,robust surveillance is essential to assess the evolution of viruses in real time.After the epidemic in Wuhan City was brought under control in 2020,several COVID-19 outbreaks in the mainland of China have been proven to relate to SARS-CoV-2 contaminated cold-chain products(5–7),while most were caused by transmission through imported cases on flights,at isolation facilities,or in designated hospitals(8–9).Therefore,genomic surveillance for SARS-CoV-2 from imported cases is of great significance for monitoring the risk of different variants that were imported into the mainland of China,assessing the risk of importation-associated domestic spread,and helping guide public health interventions.On March 17,2020,the China CDC released a notice and launched genomic surveillance for SARS-CoV-2 from imported COVID-19 cases nationwide.The laboratories of provincial CDCs were required to conduct SARS-CoV-2 whole-genome sequencing for samples from imported cases and submit the genomic sequences to the China CDC in time.This study includes the analysis of genomic surveillance data of imported SARS-CoV-2 cases of 2021 from the mainland of China. | Yenan Feng Xiang Zhao Zhixiao Chen Kai Nie Zeyuan Yin Ying Xia Ji Wang Peihua Niu Ruhan A Lili Li Dayan Wang Wenjie Tan Xuejun Ma Shiwen Wang Huanyu Wang George F.Gao Cao Chen Wenbo Xu | 2022 | China CDC weekly2022，4，31： | 1 |
| 13 | Killing reduction of 5-dimensional spacetime 显示文摘 | Yang Xuejun Ma Yongge Shao Jianbing | 2003 | Phys Rev2003，68，02： | 1 |
| 14 | Optimization of digital multi-beamforming for space-based ADS-B using distributed cooperative coevolution with an adaptive grouping strategy显示文摘Space-based Automatic Dependent Surveillance-Broadcast(ADS-B)technology can eliminate the blind spots of terrestrial ADS-B systems because of its global coverage capability.However,the space-based ADS-B system faces new problems such as extremely low Signal-toNoise Ratio(SNR)and serious co-channel interference,which result in long update intervals.To minimize the position message update interval at an update probability of 95%with full coverage constraint,this paper presents an optimization model of digital multi-beamforming for space-based ADS-B.Then,a coevolution method DECCG_A&A is proposed to enhance the optimization efficiency by using an improved adaptive grouping strategy.The strategy is based on the locations of uncovered areas and the aircraft density under the coverage of each beam.Simulation results show that the update interval can be effectively controlled to be below 8 seconds compared with other existing methods,and DECCG_A&A is superior in convergence to the Genetic Algorithm(GA)as well as the coevolution algorithms using other grouping strategies.Overall,the proposed optimization model and method can significantly reduce the update interval,thus improving the surveillance performance of space-based ADS-B for air traffic control. | Xueyuan LI Xuejun ZHANG Yuanhao TAN Jianxiang MA | 2023 | Chinese Journal of Aeronautics2023，36，10： | 1 |
| 15 | Surveillance and Analysis of SARS-CoV-2 Variant Importation—China,January–June 2022显示文摘Introduction:The severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)Omicron variant is the dominant circulating strain worldwide.To assess the importation of SARS-CoV-2 variants in the mainland of China during the Omicron epidemic,the genomic surveillance data of SARS-CoV-2 from imported coronavirus disease 2019(COVID-19)cases in the mainland of China during the first half of 2022 were analyzed.Methods:Sequences submitted from January to July 2022,with a collection date before June 30,2022,were incorporated.The proportions of SARS-CoV-2 variants as well as the relationships between the origin and destination of each Omicron imported case were analyzed.Results:4,946 sequences of imported cases were submitted from 27 provincial-level administrative divisions(PLADs),and the median submission interval was within 1 month after collection.In 3,851 Omicron sequences with good quality,1 recombinant(XU)and 4 subvariants under monitoring(BA.4,BA.5,BA.2.12.1,and BA.2.13)were recorded,and 3 of them(BA.4,BA.5,and BA.2.12.1)caused local transmissions in the mainland of China later than that recorded in the surveillance.Omicron subvariants dominated in the first half of 2022 and shifted from BA.1 to BA.2 then to BA.4 and BA.5.The percentage of BA.2 in the imported SARS-CoV-2 surveillance data was far higher than that in the Global Initiative on Sharing All Influenza Data(GISAID).The imported cases from Hong Kong Special Administrative Region,China,accounted for 32.30%of Omicron cases sampled,and 98.71%of them were BA.2.Conclusions:The Omicron variant showed the intra-Omicron evolution in the first half of 2022,and all of the Omicron subvariants were introduced into the mainland of China multiple times from multiple different locations. | Yenan Feng Xiang Zhao Zeyuan Yin Changcheng Wu Zhixiao Chen Kai Nie Ruhan A Lili Li Peihua Niu Ji Wang Yuchao Wu Shiwen Wang Dayan Wang Wenjie Tan Huanyu Wang Xuejun Ma George FGao Cao Chen Wenbo Xu | 2022 | China CDC weekly2022，4，50： | 1 |
| 16 | Treatment of the wastewater containing low-level 241 Am using flocculation-microfiltration process显示文摘 | Gao Yong Zhao Jun Zhang Guanghui Zhang Dong Cheng Weiwen Yuan Guoqi Liu Xuejun Ma Bangzhong Zeng Junhui Gu Ping | 2004 | Separation and Purification Technology2004，，2： | 1 |
| 17 | Ordered Macroporous Titanium Phosphonate Materials: Synthesis, Photocatalytic Activity, and Heavy Metal Ion Adsorption 显示文摘 | Ma Tianyi Zhang Xuejun Shao Gaosong | 2008 | J Phys Chem C2008，112，8： | 1 |
| 18 | Development of a PDRA Method for Detection of the D614G Mutation in COVID-19 Virus—Worldwide,2021显示文摘ABSTRACT Background:COVID-19 infection is a major public health problem worldwide,and the D614G mutation enhances the infectivity of COVID-19.Methods:A probe-directed recombinase amplification(PDRA)assay was discussed to detect the D614G mutation at 39℃for 30 min.The sensitivity,specificity,and reproducibility of the PDRA were evaluated by D614 and G614 recombinant plasmids.The clinical performance of PDRA assay was validated by testing of 53 previously confirmed COVID-19 positive RNAs and 10 negative samples.Direct sequencing was carried out in parallel for comparison.Result:With good reproducibility and specificity,the PDRA assay worked well with the concentration in the range of 103–107 copies/reaction.Compared with direct sequencing as a reference,the recombinase-aided amplification(RAA)assay obtained 100%sensitivity and 100%specificity using clinical samples. | Ziwei Chen Xinxin Shen Ji Wang Xiang Zhao Yuan Gao Ruiqin Zhang Jinrong Wang Leping Liu Xinmin Nie Xuejun Ma | 2021 | China CDC weekly2021，3，21： | 1 |
| 19 | Field Evaluation of a Duplex RT-RAA Assay for Rapid Detection of SARS-CoV-2--Hebei Province,China,January 2021显示文摘Introduction:Recently,a local cluster epidemic has occurred in Shijiazhuang City,Hebei Province.Failure to promptly identify patients with fever in rural areas was the major reason for this epidemic.Methods:We presented the field evaluation of a new real-time reverse transcription recombinase-aided amplification(RT-RAA)kit incorporating an endogenous internal control in a single-tube format,completed at the Hebei CDC from January 17,2021 to January 27,2021.Results:We evaluated the diagnostic performance of RT-RAA assay using automatic extracted RNA of 808 clinical samples.Compared with reverse transcriptase real-time quantitative PCR(qRT-PCR),RT-RAA kit achieved 92.41%sensitivity,98.78%specificity and a 96.29%coincidence rate,demonstrating an excellent agreement between the RTRAA assay and qRT-PCR assay.Furthermore,58 samples were extracted using a manual extraction method within 5 minutes,but only samples with high nucleic acid concentration(cycle threshold value not higher than 32)could be stably detected.Discussion:The RT-RAA is more suitable to meet the needs of rapid,sensitive,and accurate detection in community-level medical institutions. | Ruiqing Zhang Shunxiang Qi Jiandong Li Shiyou Liu Guangyue Han Xinxin Shen Qi Li Wenbo Xu Xuejun Ma | 2022 | China CDC weekly2022，4，23： | 1 |
| 20 | PVA-assisted solvothermal fabrication of tin oxide sub-microrods显示文摘 | Hua Chunxia Wua Youshi Ma Xuejun | 2004 | Journal of Crystal Growth2004，265，： | 1 |
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