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| 1 | A strategy of screening and binding analysis of bioactive components from traditional Chinese medicine based on surface plasmon resonance biosensor显示文摘Elucidating the active components of traditional Chinese medicine(TCM)is essential for understanding the mechanisms of TCM and promote its rational use as well as TCM-derived drug development.Recent studies have shown that surface plasmon resonance(SPR)technology is promising in this field.In the present study,we propose an SPR-based integrated strategy to screen and analyze the major active components of TCM.We used Radix Paeoniae Alba(RPA)as an example to identify the compounds that can account for its anti-inflammatory mechanism via tumor necrosis factor receptor type 1(TNF-R1).First,RPA extraction was analyzed using an SPR-based screening system,and the potential active ingredients were collected,enriched,and identified as paeoniflorin and paeonol.Next,the affinity constants of paeoniflorin and paeonol were determined as 4.9 and 11.8 mM,respectively.Then,SPR-based competition assays and molecular docking were performed to show that the two compounds could compete with tumor necrosis factor-a(TNF-a)while binding to the subdomain 1 site of TNF-R1.Finally,in biological assays,the two compounds suppressed cytotoxicity and apoptosis induced by TNF-a in the L929 cell line.These findings prove that SPR technology is a useful tool for determining the active ingredients of TCM at the molecular level and can be used in various aspects of drug development.The SPR-based integrated strategy is reliable and feasible in TCM studies and will shed light on the elucidation of the pharmacological mechanism of TCM and facilitate its modernization. | Diya Lv Jin Xu Minyu Qi DongyaoWang Weiheng Xu Lei Qiu Yinghua Li Yan Cao | 2022 | Journal of Pharmaceutical Analysis2022，12，3： | 3 |
| 2 | Peroxisome proliferator‐activated receptor‐γ activation inhibits hepatocellular carcinoma cell invasion by upregulating plasminogen activator inhibitor‐1显示文摘 | Xiaojuan Pang Yinna Wei Ya Zhang Minyue Zhang Yan Lu Pingping Shen | 2013 | Cancer Sci2013，，6： | 1 |
| 3 | Preparation and characterization of nano - TiO2 powder显示文摘 | BAORANG LI XIAOHUI WANG MINYU YAN | 2001 | Materials Chemistry and Physics2001，119，： | 1 |
| 4 | Influence of grain size on distribution of temperature and thermal stress in ZnO varistor ceramics显示文摘 | Qingheng Chen Jinliang He Kexiong Tan Shuiming Chen Minyu Yan Jianxin Tang | 2002 | Science in China Series E: Technolgical Science2002，，4： | 1 |
| 5 | A nonlinear neural network model of mixture of local principal component analysis : Application to handwritten digits recognition 显示文摘 | Zhang Bailing Fu Minyue Yan Hong | 2001 | Pattern Recognition2001，34，2： | 1 |
| 6 | Influence of grain size on distribution of temperature and thermal stress in ZnO varistor ceramics显示文摘 | Qingheng Chen Jinliang He Kexiong Tan Shuiming Chen Minyu Yan Jianxin Tang | 2002 | Science in China Series E: Technolgical Science2002，，4： | 1 |
| 7 | Transcriptome and weighted gene co-expression network analysis of jujube(Ziziphus jujuba Mill.)fruit reveal putative genes involved in proanthocyanin biosynthesis and regulation显示文摘Proanthocyanidin(PA)is an important bioactive compound with multiple physiological benefits in jujube(Ziziphus jujube Mill.).However,the molecular mechanisms underlying PA biosynthesis in jujube fruit have not been investigated.Here,the profiling of PA,(+)-catechin and(–)-epicatechin and transcriptome sequencing of three jujube cultivars from Xinjiang Uyghur Autonomous Region of China at five developmental stages were analyzed.The levels of total PAs and catechin exhibited a decreased trend over jujube ripening,and epicatechin content of two jujube cultivars increased first and then declined.Transcriptome analysis revealed that the differentially expressed genes(DEGs)were mainly enriched in ribosome,glycolysis/gluconeogenesis,fructose and mannose metabolism.17 DEGs encoding PAL,CHS,CHI,CHS,F3'H,LAR,ANR,C4Hs,4CLs,FLSs,DFRs and UFGTs involved in PA biosynthesis were relatively abundant.The highly transcribed LAR gene may greatly contribute to epicatechin accumulation.A weighted gene co-expression network analysis(WGCNA)was performed,and a network module including 1620 genes highly correlated with content of Pas and catechin was established.We identified 58 genes including 9 structural genes and 49 regulatory genes related to PA biosynthesis and regulation in the WGCNA module.Sixteen genes encoding 9 families of transcriptional factors(i.e.,MYB,bHLH,ERF,bZIP,NAC,SBP,MIKC,HB,WRKY)were considered as hub genes.The results of qRT-PCR analysis validating 10 genes were well consistent with the transcriptome data.These findings provide valuable knowledge to facilitate its genetic studies and molecular breeding. | Wenqiang Wang Yunfeng Pu Hao Wen Dengyang Lu Min Yan Minzhe Liu Minyu Wu Hongjin Bai Lirong Shen Cuiyun Wu | 2023 | Food Science and Human Wellness2023，12，5： | 1 |
| 8 | Handwritten digit recognition by adaptive-subspace self-organizing map显示文摘 | Bailing Zhang Minyue Fu Hong Yan Marwan A Jabri | 1999 | IEEE Transactions on neural network1999，10，4： | 1 |
| 9 | Materials Preparation and characterization of nano - TiO2 powder 显示文摘 | Li Baorang Wang Xiaohui Yan Minyu | 2003 | Chemistry and Physics2003，78，13： | 1 |
| 10 | Influence of grain size on distribution of temperature and thermal stress in ZnO varistor ceramics显示文摘 | Qingheng Chen Jinliang He Kexiong Tan Shuiming Chen Minyu Yan Jianxin Tang | 2002 | Science in China Series E: Technolgical Science2002，，4： | 1 |
| 11 | Preparation and characterization of nano-Ti02 powder显示文摘 | Baorang Li Xiaohui Wang Minyu Yan | 2002 | Materials Chemistry and Physics2002，78，1： | 1 |
| 12 | Development of a surface plasmon resonance biosensor for accurate and sensitive quantitation of small molecules in blood samples显示文摘Therapeutic drug monitoring(TDM)has played an important role in clinical medicine for precise dosing.Currently,chromatographic technology and immunoassay detection are widely used in TDM and have met most of the needs of clinical drug therapy.However,some problems still exist in practical applications,such as complicated operation and the influence of endogenous substances.Surface plasmon resonance(SPR)has been applied to detect the concentrations of small molecules,including pesticide residues in crops and antibiotics in milk,which indicates its potential for in vivo drug detection.In this study,a new SPR-based biosensor for detecting chloramphenicol(CAP)in blood samples was developed and validated using methodological verification,including precision,accuracy,matrix effect,and extraction recovery rate,and compared with the classic ultra-performance liquid chromatographyultraviolet(UPLC-UV)method.The detection range of SPR was 0.1-50 ng/mL and the limit of detection was 0.099±0.023 ng/mL,which was lower than that of UPLC-UV.The intra-day and inter-day accuracies of SPR were 98%-114% and 110%-122%,which met the analysis requirement.The results show that the SPR biosensor is identical to UPLC-UV in the detection of CAP in rat blood samples;moreover,the SPR biosensor has better sensitivity.Therefore,the present study shows that SPR technology can be used for the detection of small molecules in the blood samples and has the potential to become a method for therapeutic drug monitoring. | Minyu Qi Diya Lv Ying Zhang Dongyao Wang Xiaofei Chen Zhenyu Zhu Zhanying Hong Yifeng Chai Hai Zhang Yan Cao | 2022 | Journal of Pharmaceutical Analysis2022，12，6： | 0 |
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