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| 1 | Application of next-generation sequencing technology to precision medicine in cancer: joint consensus of the Tumor Biomarker Committee of the Chinese Society of Clinical Oncology显示文摘Next-generation sequencing(NGS) technology is capable of sequencing millions or billions of DNA molecules simultaneously.Therefore, it represents a promising tool for the analysis of molecular targets for the initial diagnosis of disease, monitoring of disease progression, and identifying the mechanism of drug resistance. On behalf of the Tumor Biomarker Committee of the Chinese Society of Clinical Oncology(CSCO) and the China Actionable Genome Consortium(CAGC), the present expert group hereby proposes advisory guidelines on clinical applications of NGS technology for the analysis of cancer driver genes for precision cancer therapy. This group comprises an assembly of laboratory cancer geneticists, clinical oncologists, bioinformaticians,pathologists, and other professionals. After multiple rounds of discussions and revisions, the expert group has reached a preliminary consensus on the need of NGS in clinical diagnosis, its regulation, and compliance standards in clinical sample collection. Moreover, it has prepared NGS criteria, the sequencing standard operation procedure(SOP), data analysis, report, and NGS platform certification and validation. | Xuchao Zhang Zhiyong Liang Shengyue Wang Shun Lu Yong Song Ying Cheng Jianming Ying Weiping Liu Yingyong Hou Yangqiu Li Yi Liu Jun Hou Xiufeng Liu Jianyong Shao Yanhong Tai Zheng Wang Li Fu Hui Li Xiaojun Zhou Hua Bai Mengzhao Wang You Lu Jinji Yang Wenzhao Zhong Qing Zhou Xuening Yang Jie Wang Cheng Huang Xiaoqing Liu Xiaoyan Zhou Shirong Zhang Hongxia Tian Yu Chen Ruibao Ren Ning Liao Chunyan Wu Zhongzheng Zhu Hongming Pan Yanhong Gu Liwei Wang Yunpeng Liu Suzhan Zhang Tianshu Liu Gong Chen Zhimin Shao Binghe Xu Qingyuan Zhang Ruihua Xu Lin Shen Yilong Wu | 2019 | Cancer Biology & Medicine2019，16，1： | 14 |
| 2 | Higher PD-1 expression concurrent with exhausted CD8+ T cells in patients with de novo acute myeloid leukemia显示文摘Objective: To investigate the association between the T cell inhibitory receptor programmed death 1(PD-1)and T cell exhaustion status in T cells from patients with de novo acute myeloid leukemia(AML) and AML in complete remission(CR).Methods:Surface expression of PD-1 and the exhaustion and immunosenescence markers CD244 and CD57 on CD3+,CD4+ and CD8+ T cells from peripheral blood samples from 20 newly diagnosed,untreated AML patients and 10 cases with AML in CR was analyzed by flow cytometry.Twenty-three healthy individuals served as control.Results:A significantly higher percentage of PD-1+ cells were found for CD3+ T cells in the de novo AML group compared with healthy controls.In addition,an increased level of PD-1+ CD8+ T cells,but not PD-1+ CD4+,was found for CD3+ T cells in the de novo AML and AML-CR samples.A higher percentage of CD244+ CD4+,CD244+ CD8+,CD57+ CD4+ and CD57+ CD8+ T cells was found in CD3+ T cells in samples from those with de novo AML compared with those from healthy controls.Strong increased PD-1+ CD244+ and PD-1+ CD57+ coexpression was found for CD4+ and CD8+ T cells in the de novo AML group compared with healthy controls.Conclusions:We characterized the major T cell defects,including co-expression of PD-1 and CD244,CD57-exhausted T cells in patients with de novo AML,and found a particular influence on CD8+ T cells,suggesting a poor anti-leukemia immune response in these patients. | Jiaxiong Tan Shaohua Chen Yuhong Lu Danlin Yao Ling Xu Yikai Zhang Lijian Yang Jie Chen Jing Lai Zhi Yu Kanger Zhu Yangqiu Li | 2017 | Chinese Journal of Cancer Research2017，29，5： | 10 |
| 3 | Guiding T lymphopoiesis from pluripotent stem cells by defined transcription factors显示文摘Achievement of immunocompetent and therapeutic T lymphopoiesis from pluripotent stem cells(PSCs)is a central aim in T cell regenerative medicine.To date,preferentially reconstituting T lymphopoiesis in vivo from PSCs remains a practical challenge.Here we documented that synergistic and transient expression of Runx1 and Hoxa9 restricted in the time window of endothelial-to-hematopoietic transition and hematopoietic maturation stages in a PSC differentiation scheme(iR9-PSC)in vitro induced preferential generation of engraftable hematopoietic progenitors capable of homing to thymus and developing into mature T cells in primary and secondary immunodeficient recipients.Single-cell transcriptome and functional analyses illustrated the cellular trajectory of T lineage induction from PSCs,unveiling the T-lineage specification determined at as early as hemogenic endothelial cell stage and identifying the bona fide pre-thymic progenitors.The induced T cells distributed normally in central and peripheral lymphoid organs and exhibited abundant TCRαβrepertoire.The regenerative T lymphopoiesis restored immune surveillance in immunodeficient mice.Furthermore,gene-edited iR9-PSCs produced tumor-specific T cells in vivo that effectively eradicated tumor cells.This study provides insight into universal generation of functional and therapeutic T cells from the unlimited and editable PSC source. | Rongqun Guo Fangxiao Hu Qitong Weng Cui Lv Hongling Wu Lijuan Liu Zongcheng Li Yang Zeng Zhijie Bai Mengyun Zhang Yuting Liu Xiaofei Liu Chengxiang Xia Tongjie Wang Peiqing Zhou Kaitao Wang Yong Dong Yuxuan Luo Xiangzhong Zhang Yuxian Guan Yang Geng Juan Du Yangqiu Li Yu Lan Jiekai Chen Bing Liu Jinyong Wang | 2020 | Cell Research2020，30，1： | 7 |
| 4 | Pathways related to PMA-differentiated THP1 human monocytic leukemia cells revealed by RNA-Seq显示文摘Previous analyses have reported that the human monocytic cell line THP1 can be differentiated into cells with macrophage-like characteristics by phorbol 12-myristate 13-acetate(PMA). However, little is known about the mechanism responsible for regulating this differentiation process. Here, we performed high-throughput RNA-Seq analysis to investigate the genes differently expressed in THP1 cells treated with and without PMA and examined those that may be responsible for the PMA-induced differentiation of monocytes into macrophages. We found 3,000 genes to be differentially expressed after PMA treatment. Gene ontology analysis revealed that genes related to cellular processes and regulation of biological processes were significantly enriched. KEGG analysis also demonstrated that the differentially expressed genes(DEGs) were significantly enriched in the PI3K/AKT signaling pathway and phagosome pathway. Importantly, we reveal an important role of the PI3K/AKT pathway in PMA-induced THP1 cell differentiation. The identified DEGs and pathways may facilitate further study of the detailed molecular mechanisms of THP1 differentiation. Thus, our results provide numerous potential therapeutic targets for modulation of the differentiation of this disease. | ZENG ChengWu WANG WenTao YU XiBao YANG LiJian CHEN ShaoHua LI YangQiu | 2015 | Science China(Life Sciences)2015，58，12： | 7 |
| 5 | Activation of transmembrane receptor tyrosine kinase DDR1-STAT3 cascade by extracellular matrix remodeling promotes liver metastatic colonization in uveal melanoma显示文摘Colonization is believed a rate-limiting step of metastasis cascade.However,its underlying mechanism is not well understood.Uveal melanoma(UM),which is featured with single organ liver metastasis,may provide a simplified model for realizing the complicated colonization process.Because DDR1 was identified to be overexpressed in UM cell lines and specimens,and abundant pathological deposition of extracellular matrix collagen,a type of DDR1 ligand,was noted in the microenvironment of liver in metastatic patients with UM,we postulated the hypothesis that DDR1 and its ligand might ignite the interaction between UM cells and their surrounding niche of liver thereby conferring strengthened survival,proliferation,sternness and eventually promoting metastatic colonization in liver.We tested this hypothesis and found that DDR1 promoted these malignant cellular phenotypes and facilitated metastatic colonization of UM in liver.Mechanistically,UM cells secreted TGF-β1 which induced quiescent hepatic stellate cells(qHSCs)into activated HSCs(aHSCs)which secreted collagen type I.Such a remodeling of extracellular matrix,in turn,activated DDR1,strengthening survival through upregulating STAT3-dependent Mcl-1 expression,enhancing sternness via upregulating STAT3-dependent S0X2,and promoting clonogenicity in cancer cells.Targeting DDR1 by using 7rh,a specific inhibitor,repressed proliferation and survival in vitro and in vivo outgrowth.More importantly,targeting cancer cells by pharmacological inactivation of DDR1 or targeting microenvironmental TGF-β1-collagen I loop exhibited a prominent anti-metastasis effect in mice.In conclusion,targeting DDR1 signaling and TGF-β1 signaling may be a novel approach to diminish hepatic metastasis in UM. | Wei Dai Shenglan Liu Shubo Wang Li Zhao Xiao Yang Jingfeng Zhou Yun Wang Jing Zhang Ping Zhang Ke Ding Yangqiu Li Jingxuan Pan | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 3 |
| 6 | Tribological behaviors of different diamond-like carbon coatings on nitride mild steel lubricated with benzotriazole-eontaining borate es- ters 显示文摘 | Jia Zhengfeng Xia Yangqiu Pang Xianjuan | 2011 | Tribology Letters2011，41，： | 1 |
| 7 | Parallel spectral clustering in distributed systems 显示文摘 | WenYen Chen Yangqiu Song Hongjie Bai | 2011 | IEEE Transac- tions on Pattern Analysis and Machine Intelligence2011，33，3： | 1 |
| 8 | TextFlow:Towards Beiter Understanding of Evolving Topics in Text显示文摘 | Weiwei Cui Shixia Liu Li Tan Conglei Shi Yangqiu Song Zekai Gao Huamin Qu Xin Tong | | 0，，12： | 1 |
| 9 | The real-time guide data fusion for an optoelectronic TH eodolite 显示文摘 | Chen Juan Huang Yangqiu | 2004 | SPIE2004，5434，： | 1 |
| 10 | Study of asphaltene dispersion and removal for high-asphaltene oil wells显示文摘Many wellbores are blocked by asphaltene deposits,which lead to production problems in the oilfield development process.In this paper,methods such as elemental analysis,and solvent extraction are adopted for the study of wellbore blockages.The content of organic matter in blockages is higher than 96% and asphaltene is the main component of the organic matter with n-heptane asphaltene content of 38%.Based on the above analyses,an agent for asphaltene dispersion and removal(named as SDJ) was developed.The performance of the SDJ agent was evaluated,and it was found that the dissolution rate of asphaltene can reach 2.9 mg.mL-1.min-1 at 60 oC.SDJ agent(1wt%) was added to crude oil with a colloid instability index greater than 0.9 can effectively inhibit asphaltene deposition in the wellbore.By the viscosity method,the dissolution amount of SDJ agent was calculated,and it was found that when the viscosity of the system is around 2,000 mPa.s(the common viscosity of crude oil),the amount of SDJ agent added to the blockage was at least 96 g per 100 g blockages.Therefore,SDJ agent has promising application for dispersion and removal of asphaltene deposition in high-asphaltene wells. | Chen Chaogang Guo Jixiang An Na Pan Yangqiu Li Yaguang Jiang Qingzhe | 2012 | Petroleum Science2012，9，4： | 1 |
| 11 | Parallel Spectral Clustering in Distributed Systems 显示文摘 | CHEN Wen-Yen SONG Yangqiu BAI Hongjie | 2011 | IEEE Transac- tions on Pattern Analysis and Machine Intelligence2011，33，3： | 1 |
| 12 | Low-valence ion addition induced more compact passive films on nickel-copper nano-coatings显示文摘Ni-Cu nano-coatings were prepared by pulsed electroplating technique in the baths containing various amount of boric acid. Their microstructure, morphologies and corrosion resistance were characterized in detail. The addition of boric acid strongly influences on the microstructure of the Ni-Cu coatings.The coating with a grain size of 130 nm, obtained from the bath containing 35 g L^-1 boric acid, shows the highest corrosion resistance. This is attributed to the low-valence Cu ion(Cu^+) additions in nickel oxide, which could significantly decrease the oxygen ion vacancy density in the passive film to form a more compact passive film. The higher Cu^+ additions and the lower diffusivity of point defects(D0) are responsible for the formation of more compact passive film on the coating obtained from the bath with 35 g L^-1 boric acid. | Quangquan Do Hongze An Guozhe Meng Weihua Li Lai-Chang Zhang Yangqiu Wang Bin Liu Junyi Wang Fuhui Wang | 2019 | Journal of Materials Science & Technology2019，35，10： | 1 |
| 13 | A unified framework for semi-supervised dimensionality reduction显示文摘 | Yangqiu Song Feiping Nie Changshui Zhang Shiming Xiang | 2008 | Pattern Recognition2008，，9： | 1 |
| 14 | Semi-supervised sub-manifold discriminant analysis显示文摘 | Yangqiu Song Feiping Nie Changshui Zhang | 2008 | Pattern Recognition Letters2008，，13： | 1 |
| 15 | Syntheti, Lubricants 显示文摘 | Ou Yangqiu Li Gang | 1995 | 22 ( 3 ) : 1 - 51995，8，： | 1 |
| 16 | 显示文摘 | LUShen—Zhou(陆神洲) YANGQiu—Hong(杨秋红) XUFeng(徐峰) eta1 | 2012 | 光学学报2012，32，3： | 1 |
| 17 | Nonformaldehyde wrinklefree garment finishing of cotton slacks 显示文摘 | Wei Weishu Yang Charles Q Jiang Yangqiu | 1999 | Textile Chem Color1999，61，1： | 1 |
| 18 | Semi- supervised Sub-manifold Discriminant Analysis 显示文摘 | Song Yangqiu Nie Feiping Zhang Changshui | 2008 | Pattern Recognition Letters2008，29，13： | 1 |
| 19 | Preparation of coal - based microfiltration carbon membrane and application in oily wastewater treatment 显示文摘 | Song Chengwen Wang Tonghua Pan Yangqiu | 2006 | Sep Purif Technol2006，51，： | 1 |
| 20 | TIARA: Interactive, Topic-Based Visual Text Summarization and Analysis 显示文摘 | Shixia Liu Michelle X Zhou Shimei Pan Yangqiu Song Weihong Qima Weijia Cai Xiaoxiao Lima | 2012 | ACM Transactions on Intelligent Systems and Technology (TIST)2012，3，2： | 1 |
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