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| 1 | Novel functional proteins interact with midkine in hepatic cancer cells显示文摘BACKGROUND: Midkine is a heparin-binding growth factor that promotes the proliferation, survival, migration and differentiation of various target cells. Midkine plays an important role in tumorigenesis and tumor progression, and is overexpressed in many human malignant tumors. Patients with high tumor midkine expression frequently have a worse prognosis than those with low expression. The present study was designed to investigate the interaction network of midkine in hepatic cancer cells, and to elucidate its role in hepatocellular carcinoma. METHODS: DNA encoding full-length midkine was cloned into pDBLeu vector to serve as bait in yeast two-hybrid screening to identify interacting proteins. Candidate proteins were examined on SC-Leu-Trp-His+3-AT (20 mmol/L) plates and assayed for X-gal activity, then sequenced and classified according to the GenBank. Finally, identified proteins were expressed by the in vitro expression system pCMVTnT, and protein interactions were confirmed by co-immunoprecipitation. RESULTS: Using the yeast two-hybrid system, we found 6 proteins that interacted with midkine: NK-kappa-B inhibitor alpha (I-κ-B-α), Dvl-binding protein naked cuticle 2, granulin, latent active TGF-β binding protein 3, latent active TGF-β binding protein 4, and phospholipid scramblase 1. In vitro co-immunoprecipitation demonstrated that all identified proteins directly interacted with midkine.CONCLUSION: The identification of midkine-interacting proteins in hepatic cancer cells indicates that midkine is a multifunctional factor that may participate in cell migration, differentiation, and proliferation, and is also associated with the multicellular response feedback during the development of hepatocellular carcinoma. | Qiang Yan, Hui-Lian Huang, Xing Yao, Jing Li, Li-Qin Li, Jing Zhong, Li-Shan Min, Li-Cheng Dai and Shu-Sen Zheng Department of Hepatobiliary and Pancreatic Surgery, Huzhou Central Hospital (Yan Q and Yao X) Huzhou Key Laboratory of Molecular Medicine, Affiliated Central Hospital of Huzhou Teachers College (Huang HL, Li J, Li LQ, Zhong J, Min LS and Dai LC), Huzhou 313000, China Division of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China (Yan Q and Zheng SS) | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，3： | 7 |
| 2 | Survival factors after resection of small hepatocellular carcinoma显示文摘Early resection of hepatocellular carcinoma is a key measure to prolong the survival of patients. This study was designed to summarize our experience in surgical resection of small hepatocellular carcinoma (HCC), and to analyze the factors influencing the postoperative survival of patients. METHODS:The clinicopathologic data of 105 patients with small HCC after resection from 1986 through 2003 were analyzed ; the patients had been followed up for more than half a year (median 33 months). Nine clinicopathologic factors, preoperative α-fetoprotein (AFP) level, liver cirrhosis, Child-Pugh score, tumor size (>2cm vs.≤2 cm) and number (single vs. multiple), capsule formation, portal vein tumor thrombi (PVTT), Edmondson tumor grade and surgical method, were analyzed by the log-rank test and the Cox proportional harzards model analysis. RESULTS:The cumulative 1-,3- and 5-year survival rates after the operation were 86.5%, 70.3% and 55.2%, respectively, and the 1-, 3- and 5-year disease-free survival rates were 78%, 58. 9% and 45. 6%, respectively. One patient died from esophagogastric varices hemorrhage in 2 weeks after reoperation. Thirty-six patients had intrahepatic recurrence or metastasis postoperatively and 34 patients died. The Kaplan-Meier method and the Cox proportional harzards model analysis indicated that poor Child-Pugh score, tumor more than 2 cm in diameter, PVTT and multiple lesions (including satellitic lesions) were adverse factors affecting postoperative survival. The Cox proportional harzards model analysis indicated that tumor size, PVTT and multiple lesions were the factors influencing postoperative disease-free survival. CONCLUSIONS:Limited hepatectomy with a margin more than 1 cm is an appropriate surgical approach. Adverse preoperative Child-Pugh score and postoperative intrahepatic recurrences are the main factors leading to the death of patients with small HCC. | Departments of Surgical Oncology (Wu FS, Zhao WH, Ma ZM, Teng LS and Wang M) and General Surgery ( Liang TB and Zheng SS) , First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，3： | 5 |
| 3 | Secretion,purification and characterization of a recombinant Aspergillus oryzae tannase in Pichia pastoris显示文摘 | Zhong XF Peng LS Zheng SL | 2004 | Protein Expression and Purification2004，36，2： | 1 |
| 4 | An epididymis-specific beta-defensin is important for the initiation of sperm maturation显示文摘 | Zhou CX Zhang YL Xiao L Zheng M Leung KM Chan MY Lo PS Tsang LL Wong HY Ho LS | | 0，，05： | 1 |
| 5 | Rhein reverses the diabetic phenotype of mesangial cells over-expressing the glucose transporter(GLUT1)by inhibiting the hexosamine pathway显示文摘 | Zheng JM Zhu JM Li LS | 2008 | Br J Pharmacol2008，153，7： | 1 |
| 6 | Systematic qualitative and quantitative assessment of fatty acids in the seeds of 60 tree peony(Paeonia section Moutan)cultivars by GC-MS显示文摘 | Li SS Yuan RY Chen LG Wang LS Hao XH Wang LJ Zheng XC Du H | 2015 | Food Chem2015，173，15： | 1 |
| 7 | NDRG1 is down-regulated in the early apoptotic event induced by camptotheein analogs: The potential role in proteolytie activation of PKCd and apoptosis显示文摘 | Zheng Y Wang LS Xia L | 2009 | Proteomics2009，9，8： | 1 |
| 8 | Human bocavirus infection in young children with acute respiratory tract infection in Lanzhou显示文摘 | Zheng LS Yuan XH Xie ZP | | 0，，02： | 1 |
| 9 | Struetural insight into the au- toinhibition mechanism of AMP-activated protein kinase 显示文摘 | Chert L Jiao ZH Zheng LS | 2009 | Na- ture2009，459，7250： | 1 |
| 10 | Efficacy of consensus in- terferon in treatment of HBeAg-positive chronic hepatitis B: amuhicentre, randomized controlled trial 显示文摘 | ZHENG YL ZHAO LS WU TX | 2009 | Virol J2009，6，14： | 1 |
| 11 | Human Bocavirus Infection in Young Children With Acute Respiratory Tract Infection in Lan zhou, China显示文摘 | Zheng LS Yuan XH Xie ZP | 2010 | J Med Vi rol2010，82，2： | 1 |
| 12 | Cranial neural crest ablation of Jagged/recapitulates the craniofacial phenotype of Alagille syndrome patients显示文摘 | Humphreys R Zheng W Prince LS | 2012 | Hum Mol Genet2012，21，6： | 1 |
| 13 | Human bocavirus infection in young children with acute respiratory tract infection in lanzhou, China 显示文摘 | Zheng LS Yuan XH Xie ZP | 2010 | J Med Virol2010，2，2： | 1 |
| 14 | Human bocavirus infection in young children with acute respiratory tract infection in Lanzhou 显示文摘 | Zheng LS Yuan XH Xie ZP | 2010 | J Med Virol2010，82，2： | 1 |
| 15 | Anthocyanins composition and an- tioxidant activity of wild Lycium mthenicum Mum from Qinghai - Tibet Plateau显示文摘 | Zheng J Ding CX Wang LS | 2011 | Food Chem2011，126，3： | 1 |
| 16 | Rhein reverses the diabetic phenotype of mesangial cells over-expressing the glucose trans- porter (GLUT1) by inhibiting the hexosamine pathway 显示文摘 | ZHENG JM ZHU JM LI LS | 2008 | Br JPharmacol2008，153，7： | 1 |
| 17 | Clinical studies in 500 patients with aort- oarteritis显示文摘 | Zheng DY Liu LS Fan DJ | 1990 | Chin Med J (Engl)1990，103，7： | 1 |
| 18 | Experimental study of repairing the defects of articular cartilage with bone marrow stromal cells 显示文摘 | Dai LS Dang QK Zheng ZG | 2000 | Chin J Clin Rehabil2000，4，： | 1 |
| 19 | Human bocavirus infection inyoung children with acute respiratory tract infection in Lanzhou China显示文摘 | Zheng LS Yuan XH Xie ZP | 2010 | Journal of Medical Virology2010，82，2： | 1 |
| 20 | An experimental study for early diagnostic features in fluorosis 显示文摘 | Wang J Zheng ZA Zhang LS | 1993 | Fluoride1993，26，： | 1 |
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