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| 1 | Alterations in metastatic properties of hepatocellular carcinoma cell following H-ras oncogene transfection显示文摘AIM To demonstrate the relationship betweenH-ras oncogene and hepatocellular carcinoma(HCC) metastasis.METHODS Activated H-ras oncogene wastransfected into SMMC 7721, a cell line derivedfrom human HCC, by calcium phosphatetransfection method. Some metastasis-relatedparameters were detected in vitro, includingadhesion assay, migration assay, expression ofcollagenase ⅣV (c ⅣV ase) and epidermal growthfactor receptor (EGFR).RESULTS The abilities of H-ras-transfected cellclones in adhesion to laminin (LN) or fibronectin(FN), migration, c Ⅳ ase secretion increasedmarkedly, and the expression of EGFR elevatedmoderately. More importantly, these alterationswere consistent positively with the expressionof p21, the protein product of H-ras oncogene.CONCLUSION H-ras oncogene could inducethe metastatic phenotype of HCC cell in vitro toraise its metastatic potential. | Qing Wang~1 Zhi Ying Lin~2 Xiao Li Feng~3 ~1Department of Microbiology,Medical Center of Fudan University.the former Shanghai Medical University,Shanghai 200032,China ~2Liver Cancer Institute,Zhongshan Hospital,Shanghai 200032,China ~3Shanghai Institute of Biochemistry,Academy Sinica,Shanghai 200031,ChinaQing Wang earned master degree from Shanghai Medical University in 1996,now a senior lecturer of microbiology,specialized in the role of oncogcncs on tumor metastasis,having 8 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 48 |
| 2 | Virulence and potential pathogenicity of coccoid Helicobacter pylori induced by antibiotics显示文摘AIM To explore the virulence and the potential pathogenicity of coccoid Helicobacter pylori (H. pylori) transformed from spiral form by exposure to antibiotic.METHODS Three strains of H. pylori, isolated from gastric biopsy specimens of confirmed peptic ulcer, were converted from spiral into coccoid from by exposure to metronidazole.Both spiral and coccoid form of H. pylori were tested for the urease activity, the adherence to Hep-2 cells and the vacuolating cytotoxicity to Hela cells, and the differences of the protein were analysed by SDS-PAGE and Western blot,The mutation of the genes including ureA, ureB,hpaA; vacA and cagA, related with virulence,was detected by means of PCR and PCR-SSCP.RESULTS In the coccoid H. pylori, the urease activity, the adherence to Hep-2 cells and the vacuolating cytotoxicity to Hela cells alldecreased. In strain F44, the rate and index of adherence reduced from 70.0% ± 5.3% to 33% ±5.1% and from 2.6 ±0.4 to 0.96 ±0.3 (P<0.01),respectively. The invasion of coccoid H. pylori into Hep-2 cell could be seen under electronmicroscope. SDS-PAGE showed that the content of the protein with the molecular weight over Mr74 000 decreased, and the hybriditional signal in band Mr 125 000 weakened, while the band Mr 110000 and Mr63000 strengthened in coccoid H. pylori as shown in Western blot. The results of PCR were all positive, and PCR-SSCP indicated that there may exist the point mutation in gene hpaA or vacA.CONCLUSION The virulence and the proteins with molecular weight over Mr74 000 in coccoid H. pylori decrease, but no deletion exists in amplification fragments from ureA, ureB, hpaA,vacA and cagA genes, suggesting that coccoid H. pylori may have potential pathogenicity. | Fei Fei She1 Dong Hui Su1 Jian Yin Lin2 Lin Ying Zhou3 1Department of Microbiology, Fujian Medical University. Fuzhou 350004, Fujian Province, China2Department of Molecular Medicine, Fujian Medical University, Fuzhou 350004, Fujian Province, China 3Laboratory of Electron Microscope, Fujian Medical University, Fuzhou 350004. Fujian Province. ChinaFei Fei She. graduated from Fujian Medical University as a postgraduate in 1991, now associate professor of microbiology and immunology, specialized in molecular biology of pathogen, having 15 papers published. | 2001 | World Journal of Gastroenterology2001，7，2： | 18 |
| 3 | Piglets cloned from induced pluripotent stem cells显示文摘 | Nana Fan Jijun Chen Zhouchun Shang Hongwei Dou Guangzhen Ji Qingjian Zou Lu Wu Lixiazi He Fang Wang Kai Liu Na Liu Jianyong Han Qi Zhou Dengke Pan Dongshan Yang Bentian Zhao Zhen Ouyang Zhaoming Liu Yu Zhao Lin Lin Chongming Zhong Quanlei Wang Shouqi Wang Ying Xu Jing Luan Yu Liang Zhenzhen Yang Jing Li Chunxia Lu Gabor Vajta Ziyi Li Hongsheng Ouyang Huayan Wang Yong Wang Yang Yang Zhonghua Liu Hong Wei Zhidong Luan Miguel A Esteban Hongkui Deng Huanming Yang Duanqing Pei Ning Li Gang Pei Lin Liu Yutao Du Lei Xiao Liangxue Lai | 2013 | Cell Research2013，23，1： | 12 |
| 4 | Pharmacokinetics of the prototype and hydrolyzed carboxylic forms of ginkgolides A, B, and K administered as a ginkgo diterpene lactones meglumine injection in beagle dogs显示文摘Ginkgo diterpene lactones meglumine injection(GDLI)is a commercially available product used for neuroprotection.However,the pharmacokinetic properties of the prototypes and hydrolyzed carboxylic forms of the primary components in GDLI,i.e.,ginkgolide A(GA),ginkgolide B(GB),and ginkgolide K(GK),have never been fully evaluated in beagle dogs.In this work,a simple,sensitive,and reliable method based on ultra-fast liquid chromatography-tandem mass spectrometry(UFLC-MS/MS)was developed,and the prototypes and total amounts of GA,GB,and GK were determined in beagle dog plasma.The plasma concentrations of the hydrolyzed carboxylic forms were calculated by subtracting the prototype concentrations from the total lactone concentrations.For the first time,the pharmacokinetics of GA,GB,and GK were fully assessed in three forms,i.e.,the prototypes,the hydrolyzed carboxylic forms,and the total amounts,after intravenous administration of GDLI in beagle dogs.It was shown that ginkgolides primarily existed in the hydrolyzed form in plasma,and the ratio of hydrolysates to prototype forms of GA and GB decreased gradually to a homeostatic ratio.All of the three forms of the three ginkgolides showed linear exposure of AUC to the dosages.GA,GB,and GK showed a constant half-life approximately 2.7,3.4,and 1.2 h,respectively,which were consistent for the forms at three dose levels(0.3,1.0,and 3.0 mg·kg^(-1))and after a consecutive injection of GDLI for 7 days(1.0 mg·kg^(-1)). | WANG Shu-Yao A Ji-Ye FEI Fei GENG Jian-Liang PENG Ying OUYANG Bing-Chen WANG Pei JIN Xiao-Liang ZHAO Yu-Qing WANG Jian-Kun GENG Ting LI Yan-Jing HUA NG Wen-Zhe WANG Zhen-Zhong XIAO Wei WANG Guang-Ji | 2017 | Chinese Journal of Natural Medicines2017，15，10： | 9 |
| 5 | Effect of Sr on microstructure and aging behavior of Mg-14Li alloys | Bin Jiang a,b,c,Ying Zeng b,Hengmei Yin a,b,Ruihong Li a,b,Fusheng Pan a,b a National Engineering Research Center for Magnesium Alloys,Chongqing University,Chongqing 400030,China b College of Materials Science and Engineering,Chongqing University,Chongqing 400030,China c New Materials Center,Chongqing Academy of Science and Technology,Chongqing 401123,China | 2012 | Progress in Natural Science:Materials International2012，22，2： | 3 |
| 6 | Landslides & Debris Flows Formation from Gravelly Soil Surface Erosion and Particle Losses in Jiangjia Ravine显示文摘Gravelly soils are made up of gravel, sand,silt and clay. They are widely used in engineering applications such as rock-fill dams with clay cores,which are the main researches at present. The strength and mechanical properties of the gravelly soils are affected by the content of coarse grain, fine particles, and their adhesive states. These Properties can be verified by laboratory unconsolidatedundrained triaxial tests with grain size less than 5 mm and by large scale direct-shear tests with original grain content. Fine particles of the loose gravelly slopes are released under rainfalls, alternated the structures and mechanical properties, even affected the slope stability. There are a series of large scale direct-shear tests with different coarse grain contents to study the influence of fine particles releasing and migration, results showed the strength behavior of the gravelly soils were affected by the coarse grain content(P5) and the inflection coarse grain contents. In order to study the erosion features of the gravelly soil slopes on rainfall conditions and the slopes stability alteration, we had carried out one sort of artificial rainfall local and model experiments, the runoff sediment contents were monitored during the experiments. Result showed that the shapes of the slopes surface transformed periodically, runoff sediment contents were divided into five phases according to the experiment phenomena, runoff sediment contents maintained downtrend during the rain time and the downtrend was obviouslyinterpreted by one descend belt no matter the rainfall intensity and the slope angels. Particle size analysis released the deposit on the slope surface lost almost all of the clay, most of the silt and sand after the experiments, this meant the fine particles releasing,migration and accumulation process on condition of rainfall resulted in the instability factor of the slopes even induced landslide or debris flow. | HU Ming-jian PAN Hua-li WEI Hou-zhen WANG Ren A Ying | 2013 | Journal of Mountain Science2013，10，6： | 3 |
| 7 | Comparison of ocular modulation transfer function determined by a ray-tracing aberrometer and a double-pass system in early cataract patients显示文摘 | Qiao Liya Wan Xiuhua Cai Xiaogu Balamurali Vasudevan Xiong Ying Tan Jiaxuan Guan Zheng David A Atchison Wang Ningli | 2014 | Chinese Medical Journal2014，，19： | 3 |
| 8 | Yohimbine protects against endotoxin-induced acute lung injury by blockade of alpha 2A adrenergic receptor in rats显示文摘 | LIN Ying ZHU Xi YAO Wan-zhen YANG Yan-lin A La-ta CHEN Li | 2011 | Chinese Medical Journal2011，，7： | 3 |
| 9 | GC-TOF/MS-based metabolomic profiling of estrogen deficiency-induced obesity in ovariectomized rats显示文摘 | Bo MA Qi ZHANG Guang-ji WANG Ji-ye A Di WU Ying LIU Bei CAO Lin-sheng LIU Ying-ying HU Yong-lu WANG Ya-ya ZHENG | 2011 | Acta Pharmacologica Sinica2011，32，2： | 3 |
| 10 | Reverse microemulsion synthesis of nanostructured complex oxides for catalytic combustion 显示文摘 | Zarur A J Ying J Y | 2000 | Nature2000，403，： | 1 |
| 11 | A saturated-fat diet aggrawates the outcome of traumatic brain injury on hippocampal plasticity and cognitive function by reducing brain-derived neurotrophic factor显示文摘 | Wu A Moheni R Ying Z | 2003 | Neuroscience2003，119，： | 1 |
| 12 | Relation between EPS adherence, viscoelastic properties, and MBR operation: biofouling study with QCM- D 显示文摘 | SWEITY A WANG Ying ALI-SHTAYEH M S | 2011 | Water Research2011，45，： | 1 |
| 13 | Active vibration control of the axially moving string using space feedward and feedback controllers 显示文摘 | Ying S Tan C A | 1996 | Journal of Vibration and Acoustics1996，118，： | 1 |
| 14 | Active Vibration control of the axially moving string using space feedward and feedback controllers显示文摘 | Ying S Tan C A | 1996 | Journal of Vibration and Acoustics1996，，118： | 1 |
| 15 | Self-assembly of a molecular crown as a structural analogue of calixarene to modify Keggin anions显示文摘 | TIAN A X LIN X L YING J | 2013 | Dalton Trans2013，42，27： | 1 |
| 16 | Blockade of CD40 - CD40 ligand Interactions protects against radiation induced pulmonary inflammation and fibrosis 显示文摘 | Adnan A Ying Z Raymond B | 1998 | Clin Immunol Immunopathol1998，89，3： | 1 |
| 17 | Reverse microemulsion synthesis of nanostructured complex oxides for catalytic combustion显示文摘 | Ying J Y | 2000 | Nature2000，403，： | 1 |
| 18 | Reverse microemulsion- mediated synthesis and structural evolution of barium hexaaluminate nanoparticles 显示文摘 | Zarur A J Hwu H H Ying J Y | 2000 | Langmuir2000，16，： | 1 |
| 19 | gamma/delta T cellsubsets in human aging using the classical alpha/beta T cell model显示文摘 | Vasudev A Ying CT Ayyadhury S | 2014 | J Leukoc Biol2014，96，4： | 1 |
| 20 | Genomic Surveillance for SARS-CoV-2 Variants of Concern from Imported COVID-19 Cases-the Mainland of China,2021显示文摘Introduction:After the epidemic in Wuhan City was brought under control in 2020,local outbreaks of coronavirus disease 2019(COVID-19)in the mainland of China were mainly due to imported COVID-19 cases.The ongoing evolution of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has continued to generate new variants.Some have been designated as variants of concern(VOCs)by the World Health Organization(WHO).To better assess the role of imported SARS-CoV-2 surveillance and the prevalence of VOCs in 2021,the genomic surveillance data of SARS-CoV-2 from imported COVID-19 cases of 2021 in the mainland of China were analyzed.Methods:The analyses included the number of sequence submissions,time of sequence deposition,and time of detection of the VOCs in order to determine the timeliness and sensitivity of the surveillance.The proportions of VOCs were analyzed and compared with data from the Global Initiative of Sharing All Influenza Data(GISAID).Results:A total of 3,355 sequences of imported cases were submitted from 29 provincial-level administrative divisions,with differences in the number of sequence submissions and median time of sequence deposition.A total of 2,388 sequences with more than 90%genomic coverage were used for lineage analysis.The epidemic trend from Alpha to Delta to Omicron in imported cases was consistent with that in the GISAID.In addition,VOCs from imported cases were usually identified after WHO designation and before causing local outbreaks.Conclusions:The global distribution of SARSCoV-2 VOCs changed rapidly in 2021.Robust genomic surveillance of the imported SARS-CoV-2 in the mainland of China is of great significance.Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the etiological agent of coronavirus disease 2019(COVID-19),is constantly mutating under the different circumstances of global transmission(1).The emerging SARS-CoV-2 variants may have potential adverse impacts on epidemic traits and severity.To some extent,it is also capable of escaping natural and vaccine-induced immunity(2–3).Some of them were designated as variants of concern(VOCs)by the World Health Organization(WHO)(4).Therefore,robust surveillance is essential to assess the evolution of viruses in real time.After the epidemic in Wuhan City was brought under control in 2020,several COVID-19 outbreaks in the mainland of China have been proven to relate to SARS-CoV-2 contaminated cold-chain products(5–7),while most were caused by transmission through imported cases on flights,at isolation facilities,or in designated hospitals(8–9).Therefore,genomic surveillance for SARS-CoV-2 from imported cases is of great significance for monitoring the risk of different variants that were imported into the mainland of China,assessing the risk of importation-associated domestic spread,and helping guide public health interventions.On March 17,2020,the China CDC released a notice and launched genomic surveillance for SARS-CoV-2 from imported COVID-19 cases nationwide.The laboratories of provincial CDCs were required to conduct SARS-CoV-2 whole-genome sequencing for samples from imported cases and submit the genomic sequences to the China CDC in time.This study includes the analysis of genomic surveillance data of imported SARS-CoV-2 cases of 2021 from the mainland of China. | Yenan Feng Xiang Zhao Zhixiao Chen Kai Nie Zeyuan Yin Ying Xia Ji Wang Peihua Niu Ruhan A Lili Li Dayan Wang Wenjie Tan Xuejun Ma Shiwen Wang Huanyu Wang George F.Gao Cao Chen Wenbo Xu | 2022 | China CDC weekly2022，4，31： | 1 |
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