|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Effect of intestinal ischemia-reperfusion injury on protein levels of leptin and orexin-A in peripheral blood and central secretory tissues显示文摘AIM: To explore the effect of intestinal ischemia-reperfusion injury on protein levels of leptin and orexin-A in peripheral blood and their central secretory tissues and to find out the role leptin and orexin-A play in acute inflammatory responses.METHODS: An intestinal ischemia-reperfusion (I/R)injury model of rats was established and rats were divided randomly into six groups: sham-operation group, 60 min ischemia/30 min reperfusion group (I60'R30'), I60'R90',I60'R150', I60'R240' and I60'R360', 9 rats each group.Two highly-sensitive radioimmunoassays for leptin and orexin-A were established and used to check the change of their concentrations in peripheral blood and central secretory tissues before and after intestinal I/R injury.RESULTS: Compared with the serum leptin level before injury, it decreased significantly in I60'R30' group and increased significantly in I60'R360' group; compared to sham-operation group after injury, serum leptin level increased significantly in I60'R360' group; compared to sham-operation group after injury, adipose leptin levels decreased significantly in I60'R30' and I60'R90' groups,while increased significantly in I60'R360' group. There was no significant difference between the expression levels of orexin-A before and after I/R injury.CONCLUSION: Leptin has a time-dependent response and orexin-A has a delayed response to acute inflammatory stimuli such as intestinal I/R injury and they may participate in metabolic disorders in injury as inflammatory cytokines. | JiLin Guang-TaoYan Xiu-HuaHao Lu-HuanWang KaiZhang HuiXue | 2005 | World Journal of Gastroenterology2005，11，7： | 31 |
| 2 | Effect of compound rhodiola sachalinensis A Bor on CCI4-induced liver fibrosis in rats and its probable molecular mechanisms显示文摘AIM: To explore the anti-fibrotic effect of a traditional Chinese medicine, compound rhodiola sachalinensis A Bor on CCl4-induced liver fibrosis in rats and its probable molecular mechanisms. METHODS: Ninety healthy male SD rats were randomly divided into three groups: normal group (n=-10), treatment group of compound rhodiola sachalinensis A Bor (n=-40) and CCl4-induced model group (n=40). The liver fibrosis was induced by CCl4 subcutaneous injection. Treatment group was administered with compound rhodiola sachalinensis A Bor (0.5 g/kg) once a day at the same time. Then the activities of several serum fibrosis-associated enzymes: alanine aminotransferase (ALT), aspartate aminotransferase (AST), N-acetyl-beta-D-glucosaminidase (β-NAG) and the levels ofserum procollagen Ⅲ (PCⅢ), collagen Ⅳ (CⅣ), hyaluronic acid (HA) were assayed. The histooathol(mical chanaes were observed with HE, VG and Masson stain. The expression of TGF-β1 mRNA,αl (I) mRNA and Na+/Ca2+ exchanger (NCX ) mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR) in situ.RESULTS: Compound rhodiola sachalinensis A Bor significantly reduced serum activities of ALT, AST, β-NAG and decreased the levels of PCⅢ, CⅣ, HA, improved the liver histopathological changes, inhibited the expression of TGF-β1 mRNA, α(1) mRNA and Na+/Ca2+ exchanger mRNA in rats. CONCLUSION: Compound rhodiola sachalinensis A Bor can intervene in CCI4-induced liver fibrosis in rats, in which potential mechanisms may be decreasing the production of TGF-β1, reducing the production of collagen, preventing the activation of hepatic stellate cell (HSC) and inhibiting theexpression of TGF-β1 mRNA, αl(I) mRNA and Na+/Ca2+ exchanger mRNA. | Chun-TaoLei Ming-DeJiang Xiao-BinChen YongZhang HuiXu ZhaoWang Xiao-LingWU Wei-ZhengZeng Pi-LongWang | 2003 | World Journal of Gastroenterology2003，9，7： | 21 |
| 3 | cagA and vacA genotype of Helicobacter pylori associated with gastric diseases in Xi'an area显示文摘AIM: To establish stock of clinical Helicobacter pylori (H.pylon) isolates, to perform cagA and vacA typing of these isolates, to evaluate the relationship between genotypes of cagA and vacA and upper gastrointestinal diseases and to assess the association of vacA genotypes with presence of the pathogenicity marker-cagA.METHODS: Clinical H.pylori strains were isolated from the antrum of 259 patients in Clumbia agar. The isolated H.pylori strains were identified by histology, and16SrRNA PCR.CagA genotypes were detected by colony hybridization, the probe was derived from the cloned plasmid PcagA, and digested by EcoRI-HindⅢ and the isolated PcagA DNA fragment was radioactively labelled by the random priming method. vacA genes types (s,m)and subtypes (s1a, s1b,s2) were typed by PCR. Vacuolating toxin was detected with neutral red absorb test. The results were treated statistically by χ2test, ttest, and rank sum test.RESULTS: A total of 192 clinical H. pylori strains were isolated and the stock of Helicobacter pylori was established. The total positive rate of cagA was 87 % in all gastric diseases,and 95 % in gastric cancer group. There was a difference between gastric cancer group and the other groups (P<0.05)except duodenal ulcer group. The expression of type s1 of vacA was more than type s2 (P<0.05), and, the expression of type m1 was equal to type m2. In gastric cancer group,there was a difference between s1a and s1b (P<0.05), and s1a was more than s1b. Vacuolating toxins were more in Xi′an area isolates.CONCLUSION: The cagA+ vacA type s1 clinical isolates are more in Xi′an area, but this can not serve as an index to predict gastric cancer. | WenQiao Jia-LuHu BingXiao, Kai-ChunWu Dao-RongPeng JohnCAtherton HuiXue | 2003 | World Journal of Gastroenterology2003，9，8： | 13 |
| 4 | The Blue-Light Receptor CRY1 Interacts with BZR1 and BIN2 to Modulate the Phosphorylation and Nuclear Function of BZR1 in Repressing BR Signaling in Arabidopsis显示文摘The blue-light receptor cryptochrome 1(CRY1)primarily mediates blue-light inhibition of hypocotyl elongation in Arabidopsis.However,the underlying mechanisms remain largely elusive.We report here that CRY1 inhibits hypocotyl elongation by repressing brassinosteroid(BR)signaling.A genetic interaction assay reveals the negative regulatory effect of CRY1 on the function of BZR1,a core transcription factor in the BR signaling pathway.We demonstrated that CRY1 interacts with the DNA-binding domain of BZR1 to interfere with the DNA-binding ability of BZR1,and represses its transcriptional activity.Furthermore,we found that CRY1 promotes the phosphorylation of BZR1 and inhibits the nuclear accumulation of BZR1.Interestingly,we discovered that CRY1 interacts with the GSK3-like kinase BIN2 and enhances the interaction of BIN2 and BZR1 in a light-dependent manner.Our findings revealed that CRY1 negatively regulates the function of BZR1 through at least two mechanisms:interfering with the DNA-binding ability of BZR1 and promoting the phosphorylation of BZR1.Therefore,we uncover a novel CRY1-BIN2-BZR1 regulatory module that mediates crosstalk between blue light and BR signaling to coordinate plant growth in Arabidopsis. | Guanhua He Jie Liu Huixue Dong Jiaqiang Sun | 2019 | Molecular Plant2019，12，5： | 8 |
| 5 | Effects of lanthanide ions on hydrolysis of phosphatidylin-ositol in human erythrocyte membranes显示文摘The effects of lanthanides on the hydrolysis of phosphatidylinositol in human erythrocyte membranes were studied. 3H-inositoI labeling chromatography and HPLC were used to determine inositol 1, 4, 5-triphosphatc and dia-cylglycerol separately, the hydrolytic products of phosphatidylinositol due to the reaction of lanthanide ions with human erythrocyte membranes. The unhydrolyzed phosphatidylinositol in membranes was also determined. The results indicate that the hydrolysis of phosphatidylinositol can be promoted by lanthanides (La3+, Ce3+, Y3+, Tb3+) and the effects of La3+ and Ce3+ are stronger than those of Y3+ and Tb3+. | Huixue Liu Jian Hu Xiangtao Liu Rongchang Li Kui Wang | 2001 | Chinese Science Bulletin2001，46，5： | 5 |
| 6 | Advances of adipose-derived mesenchymal stem cells-based biomaterial scaffolds for oral and maxillofacial tissue engineering显示文摘The management of oral and maxillofacial tissue defects caused by tumors,trauma,and congenital or acquired deformities has been a major challenge for surgeons over the last few decades.Autologous tissue transplantation,the gold standard of tissue reconstruction,is a valid method for repairing the oral and maxillofacial functions and aesthetics.However,several limitations hinder its clinical applications including complications of donor sites,limited tissue volume,and uncertain long-term outcomes.Adipose-derived mesenchymal stem cells(ADMSCs)widely exist in adipose tissue and can be easily obtained through liposuction.Like the bone marrow-derived mesenchymal stem cells(BMSCs),ADMSCs also have the multi-pluripotent potencies to differentiate into osteoblasts,chondrocytes,neurons,and myocytes.Therefore,the multilineage capacity of ADMSCs makes them valuable for cell-based medical therapies.In recent years,researchers have developed many candidates of ADMSCs-based biomaterial scaffolds to cater for the needs of oral and maxillofacial tissue engineering due to their superior performance.This review presents the advances and applications of ADMSCs-based biomaterial scaffolds,and explores their tissue engineering prospects in oral and maxillofacial reconstructions. | Tong Liu Jia Xu Xun Pan Zhangfan Ding Hao Xie Xiaoyi Wang Huixu Xie | 2021 | Bioactive Materials2021，6，8： | 5 |
| 7 | Photoexcited phytochrome B interacts with brassinazole resistant 1 to repress brassinosteroid signaling in Arabidopsis显示文摘Photoreceptor phytochrome B(phy B)mediates a variety of light responses in plants.To further elucidate the molecular mechanisms of phy B-regulated hypocotyl elongation,we performed firefly luciferase complementation imaging(LCI)screening for phy B-interacting transcription factors(TFs).LCI assays showed that phy B possibly interacts with brassinazoleresistant 1(BZR1),BZR2,AUXIN RESPONSE FACTOR 6(ARF6),and several WRKY DNA-binding TFs in a red light-dependent manner.Furthermore,biochemical assays demonstrated that photoexcited phy B specifically interacts with nonphosphorylated BZR1,the physiologically active form of a master TF in brassinosteroid(BR)signaling,and this interaction can be competitively interfered by phytochrome-interacting factor 4.Furthermore,we showed that phy B can directly interact with the DNA-binding domain of BZR1 and affect the enrichment of BZR1 on the chromatin of target genes.Moreover,our genetic evidence and RNA-seq analysis demonstrated that phy B negatively regulates BR signaling.Together,we revealed that photoexcited phy B directly interacts with the TF BZR1 to repress BR signaling in Arabidopsis. | Huixue Dong Jie Liu Guanhua He Pan Liu Jiaqiang Sun | 2020 | Journal of Integrative Plant Biology2020，62，5： | 5 |
| 8 | ELASTO-PLASTIC FEM ANALYSIS OF CYLINDER UPSETTING BETWEEN TWO FLAT PLATENS显示文摘0INTRODUCTIONCylinderupsetingisanimportantprocesintheformingtechnologyofheavyforgings.Theresearchofupsetingacylinderbetweenfl... | Sun Huixue Hu Jinhua Shi Yanguo Yanshan University | 1999 | Chinese Journal of Mechanical Engineering1999，12，1： | 5 |
| 9 | Preparation and Electrochemical Properties of 3-Pyridinyl-6-aryl-1,2,4-triazolo [3,4-b][1, 3,4]thiadiazoles显示文摘A series of 3-pyridinyl-6-aryl-l, 2, 4-triazolo[3, 4-b][1, 3, 4]thiadiazoles(PATT) were prepared, the structures were confirmed by IR and H NMR spectra. The results of cyclic 1 voltammetry measurements imply that all these compounds have a higher electron affinity (EA) than 2-(4-biphenyl)-5-(4-tert-butyl phenyl)-1, 3, 4-oxadiazole (PBD) which implies that PATT could be acting as better electron acceptors than widely used electron transporting material PBD. | TaiXIAO HuiXueLI ChunXiaTAN MiaoCHEN | 2005 | Chinese Chemical Letters2005，16，3： | 3 |
| 10 | Vanadyl ions binding to GroEL (HSP60) and inducing its depolymerization显示文摘Several vanadium compounds have been known for the hypoglycemic and anticancer effects. However, the mechanisms of the pharmacological and toxicological effects were not clear. In this work, we in- vestigated the potential targets of vanadium in mitochondria. Vanadyl ions were found to bind to mi- tochondria from rat liver with a stoichiometry of 244±58 nmol/mg protein and an apparent dissocia- tion constant (Kd) of (2.0±0.8)×10·16 mol/L. Using size exclusion chromatography, a vanadium-binding protein was isolated and identified to be the 60-kDa heat shock protein (HSP60) by mass spectrometry analysis and immunoassays. Additionally, binding of vanadyl ions was found to result in depolymeri- zation of homo-oligomeric HSP60 (GroEL). HSP60 is an indispensable molecular chaperone and in- volved in many kinds of pathogenesis of inflammatory and autoimmune diseases, e.g. type 1 diabetes. Our results suggested that HSP60 could be a novel important target involved in the biological and/or toxicological effects of vanadium compounds. | LEI WanHua LIU HuiXue ZHONG LiJun YANG XiaoDa WANG Kui | 2007 | Chinese Science Bulletin2007，52，20： | 2 |
| 11 | On the mean-square of the error term related ton ∑_(n≤x)λ~2(nj)显示文摘We prove a non-trivial upper bound for the quantity ∫X2|X ∑_(n≤x)λ~2(nj)-c(j-1)x| 2dx for j=2, 3, 4. | LAO HuiXue SANKARANARAYANAN Ayyadurai | 2011 | Science China Mathematics2011，54，5： | 1 |
| 12 | Accelerated loosely-coupled CFD/CSD method for nonlinear static aeroelasticity analysis显示文摘 | Dang Huixue Yang Zhichun Li Yi | 2010 | Aerospace Science and Technology2010，14，： | 1 |
| 13 | Ternary Ambipolar Phosphine Oxide Hosts Based on Indirect Linkage for Highly Efficient Blue Electrophosphorescence: Towards High Triplet Energy, Low Driving Voltage and Stable Efficiencies显示文摘 | DonghuiYu FangchaoZhao ChunmiaoHan HuiXu JingLi ZhenZhang ZhaopengDeng DonggeMa PengfeiYan | 2012 | Adv Mater2012，，4： | 1 |
| 14 | Quantitativestructure-toxicity relationship study of lethal concentration to tadpole(Bufo vulgaris formosus)for organophosphorous pesticides显示文摘 | Dong Yunyan Xin Jiang Shao Huixu | 2008 | Chemosphere2008，71，： | 1 |
| 15 | Wheat male-sterile 2 reduces ROS levels to inhibit anther development by deactivating ROS modulator 1显示文摘Ms2 is an important dominant male-sterile gene in wheat,but the biochemical function of Ms2 and the mechanism by which it causes male sterility remain elusive.Here,we report the molecular basis underlying Ms2-induced male sterility in wheat.We found that activated Ms2 specifically reduces the reactive oxygen species(ROS)signals in anthers and thereby induces termination of wheat anther development at an early stage.Furthermore,our results indicate that Ms2 is localized in mitochondria,where it physically interacts with a wheat homolog of ROS modulator 1(TaRomo1).Romo1 positively regulates the ROS levels in humans but has never been studied in plants.We found that single amino acid substitutions in the Ms2 protein that rescue the ms2 male-sterile phenotype abolish the interaction between Ms2 and TaRomo1.Significantly,Ms2 promotes the transition of TaRomo1 proteins from active monomers to inactive oligomers.Taken together,our findings unravel the molecular basis of Ms2-induced male sterility and reveal a regulatory mechanism in which ROS act as essential signals guiding the anther development program in wheat. | Jie Liu Chuan Xia Huixue Dong Pan Liu Ruizhen Yang Lichao Zhang Xu Liu Jizeng Jia Xiuying Kong Jiaqiang Sun | 2022 | Molecular Plant2022，15，9： | 1 |
| 16 | Repair for hypospadias: experience of two decades 显示文摘 | Yudong He Jun Lu | 1999 | Asian J Surg1999，22，1： | 1 |
| 17 | Experimental research on the relationship between bypass diode configuration of photovoltaic module and hot spot generation显示文摘A hot spot is a reliability problem in photovoltaic(PV) modules where a mismatched or shaded cell heats up significantly and degrades the PV module output power performance. High PV cell temperature due to a hot spot can damage the cell encapsulate and lead to second breakdown, which both cause permanent damage to the PV module. In present systems, bypass diodes are used to mitigate the hot spot problem. In this work, five commercial polysilicon P V modules configured with different numbers of bypass diodes are used to study the influence of bypass diodes on the reverse bias voltage of a shaded cell and the resulting hot spot phenomenon. The reverse bias voltage of the shaded cell, and the hot spot probability and severity decrease as the number of bypass diodes increases. Negative terminal voltage of a shaded cell accompanied by a switched-off bypass diode are the necessary condition for hot spot generation. In an extreme case where each cell has an individual bypass diode in a P V module, it still cannot avoid the hazards of a hot spot under the shading areas of 5-7 cm2, but the probability of a hot spot is reduced to a minimum of 0.41%. | Cong Gao Peng Liang Huixue Ren Peide Han | 2018 | Journal of Semiconductors2018，39，12： | 1 |
| 18 | Cloning and expression of mouse peroxiredoxin I in 1EC-6 Cells显示文摘AIM: To clone and express mouse peroxiredoxin Ⅰ in IEC-6 cells.METHODS: Total RNAs were isolated from cultured IEC-6 cells, and the coding region of peroxiredoxin I was amplified by RT-PCR. After it was cloned into T-vector and sequenced,pSG5 was used to transiently express peroxiredoxin I in IEC-6 by liposome-mediated transfection, and the expression of peroxiredoxin I was evaluated by RT-PCRand Western blot. RESULTS: A DNA fragment about 750 bp was amplified from total RNAs of IEC-6 cells using specific primers of peroxiredoxin Ⅰ. The sequencing confirmed the coding region was successfully cloned into T-vector, which was completely coincident with the sequence in GeneBank. After the EcoRI-BamHI fragment of T-vector containing peroxiredoxin I was inserted into pSG5, the recombinant plasmid was transferred to IEC-6 cells. RT-PCR assay showed that a DNA fragment of 930 bp could be amplified,which indicated the transcription of pSG5-Prx. Western blot confirmed the expression of peroxiredoxin Ⅰ in IEC-6 cells.CONCLUSION: Mouse peroxiredoxin I can be successfully expressed in IEC-6 cells. | BoZhang Yong-PingSu TaoWang Feng-ChaoWang Guo-PingAi HuiXu Jun-PingWang Yue-ShengHuang Jian-XinJiang | 2004 | World Journal of Gastroenterology2004，10，14： | 1 |
| 19 | Preparation of chitosan/silk fibroin blending membrane fixed with alginate dialdehyde for wound dressing 显示文摘 | GU Zhipeng XIE Huixu HAUNG Chengcheng | 2013 | International Journal of Biological Macromolecules2013，58，： | 1 |
| 20 | (h0,h,M0)-Uniform stability properties for nonliear differential systerms显示文摘 | Lao Huixue Fu Xilin | 2002 | Vietnam Journal of Mathematics2002，30，2： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员