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| 1 | A NAC transcription factor, NOR-like1, is a new positive regulator of tomato fruit ripening显示文摘Ripening of the model fruit tomato(Solanum lycopersicum)is controlled by a transcription factor network including NAC(NAM,ATAF1/2,and CUC2)domain proteins such as No-ripening(NOR),SlNAC1,and SlNAC4,but very little is known about the NAC targets or how they regulate ripening.Here,we conducted a systematic search of fruit-expressed NAC genes and showed that silencing NOR-like1(Solyc07g063420)using virus-induced gene silencing(VIGS)inhibited specific aspects of ripening.Ripening initiation was delayed by 14 days when NOR-like1 function was inactivated by CRISPR/Cas9 and fruits showed obviously reduced ethylene production,retarded softening and chlorophyll loss,and reduced lycopene accumulation.RNA-sequencing profiling and gene promoter analysis suggested that genes involved in ethylene biosynthesis(SlACS2,SlACS4),color formation(SlGgpps2,SlSGR1),and cell wall metabolism(SlPG2a,SlPL,SlCEL2,and SlEXP1)are direct targets of NOR-like1.Electrophoretic mobility shift assays(EMSA),chromatin immunoprecipitation-quantitative PCR(ChIP-qPCR),and dual-luciferase reporter assay(DLR)confirmed that NOR-like1 bound to the promoters of these genes both in vitro and in vivo,and activated their expression.Our findings demonstrate that NOR-like1 is a new positive regulator of tomato fruit ripening,with an important role in the transcriptional regulatory network. | Ying Gao Wei Wei Xiaodan Zhao Xiaoli Tan Zhongqi Fan Yiping Zhang Yuan Jing Lanhuan Meng Benzhong Zhu Hongliang Zhu Jianye Chen Cai-Zhong Jiang Donald Grierson Yunbo Luo Da-Qi Fu | 2018 | Horticulture Research2018，5，1： | 9 |
| 2 | Secalonic acid D induces cell apoptosis in both sensitive and ABCG2-overexpressing multidrug resistant cancer cells through upregulating c-Jun expression显示文摘Secalonic acid D(SAD) could inhibit cell growth in not only sensitive cells but also multidrug resistant(MDR) cells. However, the molecular mechanisms need to be elucidated. Here, we identified that SAD possessed potent cytotoxicity in 3 pairs of MDR and their parental sensitive cells including S1-MI-80 and S1,H460/MX20 and H460, MCF-7/ADR and MCF-7 cells. Furthermore, SAD induced cell G2/M phase arrest via the downregulation of cyclin B1 and the increase of CDC2 phosphorylation. Importantly, JNK pathway upregulated the expression of c-Jun in protein level and increased c-Jun phosphorylation induced by SAD, which was linked to cell apoptosis via c-Jun/Src/STAT3 pathway. To investigate the mechanisms of upregulation of c-Jun protein by SAD, the mR NA expression level and degradation of c-Jun were examined. We found that SAD did not alter the mR NA level of c-Jun but inhibited its proteasome-dependent degradation. Taken together, these results implicate that SAD induces cancer cell death through c-Jun/Src/STAT3 signaling axis by inhibiting the proteasome-dependent degradation of c-Jun in both sensitive cells and ATP-binding cassette transporter sub-family G member 2(ABCG2)-mediated MDR cells. | Hong Zhang Liyan Huang Liyang Tao Jianye Zhang Fang Wang Xu Zhang Liwu Fu | 2019 | Acta Pharmaceutica Sinica B2019，9，3： | 5 |
| 3 | Perspective on gallium-based room temperature liquid metal batteries显示文摘Recent years have witnessed a rapid development of deformable devices and epidermal electronics that are in urgent request for flexible batteries.The intrinsically soft and ductile conductive electrode materials can offer pivotal hints in extending the lifespan of devices under frequent deformation.Featuring inherent liquidity,metal-licity,and biocompatibility,Ga-based room-temperature liquid metals(GBRTLMs)are potential candidates to fulfill the requirement of soft batteries.Herein,to illustrate the glamour of liquid components,high-temperature liquid metal batteries(HTLMBs)are briefly summarized from the aspects of principle,application,advantages,and drawbacks.Then,Ga-based liquid metals as main working electrodes in primary and secondary batteries are reviewed in terms of battery configurations,working mechanisms,and fiinctions.Next,Ga-based liquid metals as auxiliary working electrodes in lithium and nonlithium batteries are also discussed,which work as functional self-healing additives to alleviate the degradation and enhance the durability and capacity of the battery system.After that,Ga-based liquid metals as interconnecting electrodes in multi-scenarios including photovoltaics solar cells,generators,and supercapacitors(SCs)are interpreted,respectively.The summary and perspective of Ga-based liquid metals as diverse battery materials are also focused on.Finally,it was suggested that tremendous endeavors are yet to be made in exploring the innovative battery chemistry,inherent reaction mechanism,and multifunctional integration of Ga-based liquid metal battery systems in the coining future. | Zerong XING Junheng FU Sen CHEN Jianye GAO Ruiqi ZHAO Jing LIU | 2022 | Frontiers in Energy2022，16，1： | 3 |
| 4 | Liquid metal hydraulics paradigm:Transmission medium and actuation of bimodal signals显示文摘In this article,room temperature gallium-based fluids(RTGFs)with unique thermal and conductive properties are proposed as a transmission fluid for the force carrying medium of hydraulics,which rectify the lack of the multi-functional hydraulic roles of liquid metal(LM).The typical physical properties of RTGFs and comparative conventional hydraulic fluids(commercial hydraulic oil and deionized water),such as thermal stability and rheological characteristics,are evaluated.Experimental and numerical methods,then,were adopted to clarify the force transmission performance of RTGFs and commercial hydraulic oils,as well as the influence of temperature fields on the viscosity of fluids.The results disclosed that the advantages of inherent flame resistance,wide liquid temperature range,and the viscosity changing slightly with temperature made RTGFs potential in finding efficient application in the hydraulics as new working fluids.Finally,for illustration,the rigid and flexible actuators driven by RTGFs were designed as hydraulic fluid and demonstrated their capabilities in grasping objects with various shapes and weights,respectively.And the tunable stiffness of such a flexible actuator was enabled via the solid-liquid phase change of LM.Additionally,a frequency-adjustable antenna was manufactured and showcased owing to the introduced transformable electromagnetic behaviors of LM.Overall,the gallium-based LM fluids with thermoelectrical and mechanical multimodal signal medium would serve as a potential candidate for future complex multifunctional signal transmission systems. | FU JunHeng GAO JianYe QN Peng LI DongDong YU DeHai SUN Peng HE ZhiZhu DENG ZhongShan LIU Jing | 2022 | Science China(Technological Sciences)2022，65，1： | 1 |
| 5 | Dynamic phosphorylation of CENP-N by CDK1 guides accurate chromosome segregation in mitosis显示文摘In mitosis,accurate chromosome segregation depends on the kinetochore,a supermolecular machinery that couples dynamic spin-dle microtubules to centromeric chromatin.However,the structure–activity relationship of the constitutive centromere-associated network(CCAN)during mitosis remains uncharacterized.Building on our recent cryo-electron microscopic analyses of human CCAN structure,we investigated how dynamic phosphorylation of human CENP-N regulates accurate chromosome segregation.Our mass spectrometric analyses revealed mitotic phosphorylation of CENP-N by CDK1,which modulates the CENP-L–CENP-N interaction for accurate chromosome segregation and CCAN organization.Perturbation of CENP-N phosphorylation is shown to prevent proper chromosome alignment and activate the spindle assembly checkpoint.These analyses provide mechanistic insight into a previously undefined link between the centromere–kinetochore network and accurate chromosome segregation. | Ran Liu Zhen Dou Tian Tian Xinjiao Gao Lili Chen Xiao Yuan Chunyue Wang Jiahe Hao Ping Gui McKay Mullen Felix Aikhionbare Liwen Niu Guoqiang Bi Peng Zou Xuan Zhang Chuanhai Fu Xuebiao Yao Jianye Zang Xing Liu | 2023 | Journal of Molecular Cell Biology2023，15，6： | 0 |
| 6 | The key roles of cancer stem cell-derived extracellular vesicles显示文摘Cancer stem cells(CSCs),the subpopulation of cancer cells,have the capability of proliferation,self-renewal,and differentiation.The presence of CSCs is a key factor leading to tumor progression and metastasis.Extracellular vesicles(EVs)are nano-sized particles released by different kinds of cells and have the capacity to deliver certain cargoes,such as nucleic acids,proteins,and lipids,which have been recognized as a vital mediator in cell-to-cell communication.Recently,more and more studies have reported that EVs shed by CSCs make a significant contribution to tumor progression.CSCs-derived EVs are involved in tumor resistance,metastasis,angiogenesis,as well as the maintenance of sternness phenotype and tumor immunosuppression microenvironment.Here,we summarized the molecular mechanism by which CSCs-derived EVs in tumor progression.We believed that the fully understanding of the roles of CSCs-derived EVs in tumor development will definitely provide new ideas for CSCs-based therapeutic strategies. | Chaoyue Su Jianye Zhang Yosef Yarden Liwu Fu | 2021 | Signal Transduction and Targeted Therapy2021，6，4： | 0 |
| 7 | Hierarchical dual-porous hydroxyapatite doped dendritic mesoporous silica nanoparticles based scaffolds promote osteogenesis in vitro and in vivo显示文摘Biomaterial based scaffolds for treating large bone defects require excellent biocompatibility and osteoconductivity.Here we report on the fabrication of hydroxyapatite-dendritic mesoporous silica nanoparticles(HA-DMSN)based scaffolds with hierarchical micro-pores(5µm)and nano-pores(6.4 nm),and their application for bone regeneration.The in vitro studies demonstrated good biocompatibility of dissolution extracts,as well as enhanced osteogenic potential indicated by dose-dependent upregulation of bone marker gene expression(osteocalcin gene(OCN),osteopontin gene(OPN),collagen type I alpha 1 gene(CoL1A1),runt-related transcription factor 2 gene(RUNX2),and integrin-binding sialoprotein gene(IBSP)),alkaline phosphatise(ALP)activity,and alizarin red staining.The in vivo studies showed that HA-DMSN scaffolds significantly increased bone formation in a rat cranial bone defect model after 4 weeks healing.Our study provides a simple method to fabricate promising inorganic scaffolds with hierarchical pores for bone tissue engineering. | Chang Lei Yuxue Cao Sepanta Hosseinpour Fang Gao Jingyu Liu Jianye Fu Reuben Staples Saso Ivanovski Chun Xu | 2021 | Nano Research2021，14，3： | 0 |
| 8 | Small-molecule agents for cancer immunotherapy显示文摘Cancer immunotherapy,exemplified by the remarkable clinical benefits of the immune checkpoint blockade and chimeric antigen receptor T-cell therapy,is revolutionizing cancer therapy.They induce long-term tumor regression and overall survival benefit in many types of cancer.With the advances in our knowledge about the tumor immune microenvironment,remarkable progress has been made in the development of small-molecule drugs for immunotherapy.Small molecules targeting PRR-associated pathways,immune checkpoints,oncogenic signaling,metabolic pathways,cytokine/chemokine signaling,and immune-related kinases have been extensively investigated.Monotherapy of smallmolecule immunotherapeutic drugs and their combinations with other antitumor modalities are under active clinical investigations to overcome immune tolerance and circumvent immune checkpoint inhibitor resistance.Here,we review the latest development of small-molecule agents for cancer immunotherapy by targeting defined pathways and highlighting their progress in recent clinical investigations. | Fang Wang Kai Fu Yujue Wang Can Pan Xueping Wang Zeyu Liu Chuan Yang Ying Zheng Xiaopeng Li Yu Lu Kenneth Kin Wah To Chenglai Xia Jianye Zhang Zhi Shi Zeping Hu Min Huang Liwu Fu | 2024 | Acta Pharmaceutica Sinica B2024，14，3： | 0 |
| 9 | Silica Nanoparticles with Virus-Mimetic Spikes Enable Efficient siRNA Delivery In Vitro and In Vivo显示文摘Oligonucleotide-based therapy has experienced remarkable development in the past 2 decades,but its broad applications are severely hampered by delivery vectors.Widely used viral vectors and lipid nanovectors are suffering from immune clearance after repeating usage or requiring refrigerated transportation and storage,respectively.In this work,amino-modified virus-mimetic spike silica nanoparticles(NH_(2)-SSNs)were fabricated using a 1-pot surfactant-free approach with controlled spike lengths,which were demonstrated with excellent delivery performance and biosafety in nearly all cell types and mice.It indicated that NH2-SSNs entered cells by spike-dependent cell membrane docking and dynamin-dependent endocytosis.The positively charged spikes with proper length on the surface can facilitate the efficient encapsulation of RNAs,protect the loaded RNAs from degradation,and trigger an early endosome escape during intracellular trafficking,similarly to the cellular internalization mechanism of virions.Regarding the fantastic properties of NH_(2)-SSNs in nucleic acid delivery,it revealed that nanoparticles with solid spikes on the surface would be excellent vehicles for gene therapy,presenting self-evident advantages in storage,transportation,modification,and quality control in large-scale production compared to lipid nanovectors. | Jianye Fu Wenwei Han Xue Zhang Yutong Sun Rajendra Bhadane Bo Wei Li Li Liangmin Yu Jinbo Yang Jessica MRosenholm Outi MHSalo-Ahen Taojian Fan Bin Zhang Wageh Swelm Ahmed AAl-Ghamdi Lin Xia Han Zhang Meng Qiu Hongbo Zhang Xin Wang | 2023 | Research2023，，3： | 0 |
| 10 | First Beam Commissioning of SSC-Linac as an Injector of SSC显示文摘The construction of the SSC-Linac has made significant progress in 2019.The first beam commissioning of SSC-Linac as an injector of the Separated Sector Cyclotron(SSC)was performed succssfully with ^(40)Ar^(7+) beam and the particle energy of 5.98 MeV/u was obtained at the exit of SSC on December 17,2019. | Du Heng Yin Xuejun Yang Weiqing Li Zhongshan Kong Qiyu Li Xiaoni Yang Shengli Qian Cheng WangHaoning Cong Yan Jin Peng Zhang Ruifeng Han Xiaodong Fu Xin Wang Kai Jing Long Zhang Yong Li Lili Xie Wenjun Xu Xiaowei Wang Shaoming Chen Wenjun Liu Xiaojun Yue Min An Jingrui Zhang Xiang Yan Huaihai Feng Anhui Zhang Shuai Zhang Yongbo Sun Peng Zhu Tieming Sha Xiaoping Xu Jianye Sun Xiaolong Su Xuemin Li Anping Yan Tailai Meng Jun Zhang Wei Wu Junxia Yao Qinggao Yang Yaqing Xu Zhe Sun Liangting Gao Daqing Yuan Youjin Yang Jiancheng Xia Jiawen | 2019 | IMP & HIRFL Annual Report2019，，1： | 0 |
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