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| 1 | Effects of Xinfeng capsule on pulmonary function based on treg-mediated notch pathway in a rat model of adjuvant arthritis显示文摘OBJECTIVE:To observe the impact of xinfeng xapsule(XFC) on pulmonary function in a rat model of adjuvant arthritis(AA) and to investigate the mechanism of action.METHODS:Forty rats were randomly divided into four groups of ten:normal control(NC);model control(MC);tripterygium glycosides tablet(TPT);and xinfeng capsule(XFC).Except for the NC group,AA was induced in all rats by intracutaneous injection of 0.1 mL Freund's complete adjuvant in the right paw on the 19th day.NC and MC groups were given(0.9%) physiological saline.The TPT and XFC groups were given TPT(10 mg/kg) and XFC(1.2 g/kg),respectively.Thirty days after administration,changes in paw edema(E),the arthritis index(AI),pulmonary function,levels of regulatory T-cells(Treg),ultrastructure of lung tissue,and expression of Notch receptors and ligands in lung tissue were observed.RESULTS:In the MC group,E and the AI were increased and pulmonary function significantly decreased;the structure of alveolar type-II cells was damaged;ratios of Treg in peripheral blood were reduced;and expression of Notch receptors such as Notch3 and Notch4 and ligands such as Delta1 in lung tissue were significantly increased whereas expression of Notch1,Jagged1 and Jagged2 were significantly decreased.After intervention with XFC,E and the AI were decreased;pulmonary function was enhanced;the structure of alveolar type-II cells was improved;and expression of Treg,Notch1,Jagged1,Jagged2 was elevated,whereas that of Notch3,Notch4 and Delta1 was reduced.CONCLUSION:XFC can not only inhibit E and the AI and improve joint symptoms,it can also improve pulmonary function and reduce inflammation in lung tissue.These actions could be carried out through increases in the expression of Treg,Notch receptors(Notch1) and ligands(Jagged1,Jagged2),and reductions in the expression of Notch3,Notch4 and Delta1.These phenomena would reduce the deposition of immune complexes and the inflammatory response in lung tissue,thereby improving joint symptoms and pulmonary function. | Lei Wan Jian Liu Chuanbing Huang Yuan Wang Xi Shen Wandong Zhang Guizheng Wang Haixia Fan Yao Ge Ruilian Chen Yunxiang Cao Ruikai Zong Li Lei | 2012 | Journal of Traditional Chinese Medicine2012，32，3： | 17 |
| 2 | The mechanosensitive lncRNA Neat1 promotes osteoblast function through paraspeckle-dependent Smurf1 mRNA retention显示文摘Mechanical stimulation plays an important role in bone remodeling. Exercise-induced mechanical loading enhances bone strength,whereas mechanical unloading leads to bone loss. Increasing evidence has demonstrated that long noncoding RNAs(lnc RNAs) play key roles in diverse biological, physiological and pathological contexts. However, the roles of lnc RNAs in mechanotransduction and their relationships with bone formation remain unknown. In this study, we screened mechanosensing lnc RNAs in osteoblasts and identified Neat1, the most clearly decreased lnc RNA under simulated microgravity. Of note, not only Neat1 expression but also the specific paraspeckle structure formed by Neat1 was sensitive to different mechanical stimulations, which were closely associated with osteoblast function. Paraspeckles exhibited small punctate aggregates under simulated microgravity and elongated prolate or larger irregular structures under mechanical loading. Neat1 knockout mice displayed disrupted bone formation, impaired bone structure and strength, and reduced bone mass. Neat1 deficiency in osteoblasts reduced the response of osteoblasts to mechanical stimulation. In vivo, Neat1 knockout in mice weakened the bone phenotypes in response to mechanical loading and hindlimb unloading stimulation. Mechanistically, paraspeckles promoted nuclear retention of E3 ubiquitin ligase Smurf1 m RNA and downregulation of their translation, thus inhibiting ubiquitination-mediated degradation of the osteoblast master transcription factor Runx2, a Smurf1 target. Our study revealed that Neat1 plays an essential role in osteoblast function under mechanical stimulation, which provides a paradigm for the function of the lnc RNA-assembled structure in response to mechanical stimulation and offers a therapeutic strategy for long-term spaceflight-or bedrest-induced bone loss and age-related osteoporosis. | Caizhi Liu Xingcheng Gao Yuheng Li Weijia Sun Youjia Xu Yingjun Tan Ruikai Du Guohui Zhong Dingsheng Zhao Zizhong Liu Xiaoyan Jin Yinlong Zhao Yinbo Wang Xinxin Yuan Junjie Pan Guodong Yuan Youyou Li Wenjuan Xing Guanghan Kan Yanqing Wang Qi Li Xuan Han Jianwei Li Shukuan Ling Yingxian Li | 2022 | Bone Research2022，10，2： | 3 |
| 3 | Hormones induce the formation of luminal-derived basal cells in the mammary gland显示文摘In the mammary gland, it is widely believed that the luminal cells are unipotent after birth, contributing only to the luminal compartment in normal development. Here, by lineage tracing, we uncovered an unexpected potential of luminal cells that can give rise to basal cells during pregnancy. These luminal-derived basal cells (LdBCs) persisted through mammary regression and gen erated more proge ny in successive rounds of preg nan cies. LdBCs express basal markers as well as estroge n receptor a (ERa). I n ovariectomized (OVX) mice, stimulation with estrogen and progesterone promoted the formation of LdBCs. In serial transplantation assays, LdBCs were able to rec on stitute new mammary glands in a hormone-depende nt manner. Tran scriptome an alysis and genetic experiments suggest that Wnt/p-catenin signaling is essential for the formation and maintenance of LdBCs. Our data uncover an unexpected bi-potency of luminal cells in a physiological context. The discovery of ERa+ basal cells, which can respond to hormones and are endowed with stem cell-like regenerative capacity in parous mammary gland, provides new insights into the association of hormones and breast cancer. | Wenqian Song Ran Wang Weimin Jiang Qi Yin Guangdun Peng Ruikai Yang Qing Cissy Yu Jianfeng Chen Jingsong Li Tom H. Cheung Naihe Jing Yi Arial Zeng | 2019 | Cell Research2019，29，3： | 2 |
| 4 | Protein C receptor is a therapeutic stem cell target in a distinct group of breast cancers显示文摘Breast cancer is a heterogeneous disease.In particular,triple-negative breast cancer(TNBC)comprises various molecular subgroups with unclear identities and currently has few targeted treatment options.Our previous study identified protein C receptor(Procr)as a surface marker on mammary stem cells(MaSCs)located in the basal layer of the normal mammary gland.Given the possible connection of TNBC with basal layer stem cells,we conducted comparative analyses of Procr in breast cancers of mouse and human origin.In mouse mammary tumors,we showed that Procr+cells are enriched for cancer stem cells(CSCs)in Wnt1 basal-like tumors,but not in Brea basal-like tumors or PyVT luminal tumors.In human cancers,PROCR was robustly expressed in half of TNBC cases.Experiments with patient-derived xenografts(PDXs)revealed that PROCR marks CSCs in this discrete subgroup(referred to as PROCR+TNBC).Interfering with the function of PROCR using an inhibitory nanobody reduced the CSC numbers,arrested tumor growth and prevented rapid tumor recurrence.Our data suggest a key role of MaSC in breast tumorigenesis.Moreover,our work indicates that PROCR can be used as a biomarker to stratify TNBC into clinically relevant subgroups and may provide a novel targeted treatment strategy for this clinically important tumor subtype. | Daisong Wang Xin Hu Chunye Liu Yingying Jia Yiqin Bai Cheguo Cai Jingqiang Wang Lanyue Bai Ruikai Yang ChangDong Lin Yi-Rong Liu Shan Li Feng Qiao Ling Yao Li Chen Gaoxiang Ge Hai Jiang Dianfan Li Lin Li JianFeng Chen Zhi-Ming Shao Yi Arial Zeng | 2019 | Cell Research2019，29，10： | 2 |
| 5 | Effect of Nanobubble Evolution on Hydrate Process: A Review显示文摘As a huge reserve for potential energy, natural gas hydrates(NGHs) are attracting increasingly extra attentions, and a series of researches on gas recovery from NGHs sediments have been carried out. But the slow formation and dissociation kinetics of NGHs is a major bottleneck in the applications of NGHs technology. Previous studies have shown that nanobubbles, which formed from melt hydrates, have significant promotion effects on dissociation and reformation dynamics of gas hydrates. Nanobubbles can persist for a long time in liquids, disaccording with the standpoint of classical thermodynamic theories, thus they can participate in the hydrate process. Based on different types of hydrate systems(gas + water, gas +water +inhibitors/promoters, gas + water + hydrophilic/hydrophobic surface), the effects of nanobubble evolution on nucleation, dissociation, reformation process and 'memory effect' of gas hydrates are discussed in this paper. Researches on the nanobubbles in hydrate process are also summarized and prospected in this study. | ZHANG Yue ZHAO Li DENG Shuai ZHAO Ruikai NIE Xianhua LIU Yinan | 2019 | Journal of Thermal Science2019，28，5： | 1 |
| 6 | Review on Applications of Zeotropic Mixtures显示文摘Compared with the pure fluids,the zeotropic mixtures can balance the requirements of environmental protection,heat source matching and system safety,and exhibit excellent thermodynamic performance.However,compared to the widespread applications of pure fluids,zeotropic mixtures are rarely exploited in thermodynamic cycles,and there is a lack of targeted summary on refrigeration systems,organic Rankine cycle systems and combined power and refrigeration systems.In the recent years,zeotropic mixtures are developing at an unprecedented pace,while the working fluids components are inevitably explored in the process.In this paper,the research progress of zeotropic mixtures in the field of refrigeration systems,organic Rankine cycle systems and combined power and refrigeration systems are reviewed.Based on the review of zeotropic working mixtures,the reasonable predictions can be proposed.In the future,environmental problems will still be one of the most important concerned issues.Therefore,the zeotropic mixtures consisting of natural hydrocarbons and carbon dioxide,which are environmentally friendly,have great potential for development.Furthermore,zeotropic mixtures of natural working fluids can improve comprehensive energy efficiency of combined systems and will play an important role in future carbon emission reduction technologies. | BAI Mengjie ZHAO Li ZHAO Ruikai | 2022 | Journal of Thermal Science2022，31，2： | 1 |
| 7 | Author Correction:Hormones induce the formation of luminal-derived basal cells in the mammary gland显示文摘In the initial published version of this article,there was a mistake in one author name(Jingsong Li).The correct name should be“Jinsong Li”.This correction does not affect the description of the results or the conclusions of this work. | Wenqian Song Ran Wang Weimin Jiang Qi Yin Guangdun Peng Ruikai Yang Qing Cissy Yu Jianfeng Chen Jingsong Li Tom H.Cheung Naihe Jing Yi Arial Zeng | 2019 | Cell Research2019，29，4： | 1 |
| 8 | HuR-mediated nucleocytoplasmic translocation of HOTAIR relieves its inhibition of osteogenic differentiation and promotes bone formation显示文摘Bone marrow mesenchymal stem cell(BMSC)osteogenic differentiation and osteoblast function play critical roles in bone formation,which is a highly regulated process.Long noncoding RNAs(lncRNAs)perform diverse functions in a variety of biological processes,including BMSC osteogenic differentiation.Although several studies have reported that HOX transcript antisense RNA(HOTAIR)is involved in BMSC osteogenic differentiation,its effect on bone formation in vivo remains unclear.Here,by constructing transgenic mice with BMSC(Prx1-HOTAIR)-and osteoblast(Bglap-HOTAIR)-specific overexpression of HOTAIR,we found that Prx1-HOTAIR and Bglap-HOTAIR transgenic mice show different bone phenotypes in vivo.Specifically,Prx1-HOTAIR mice showed delayed bone formation,while Bglap-HOTAIR mice showed increased bone formation.HOTAIR inhibits BMSC osteogenic differentiation but promotes osteoblast function in vitro.Furthermore,we identified that HOTAIR is mainly located in the nucleus of BMSCs and in the cytoplasm of osteoblasts.HOTAIR displays a nucleocytoplasmic translocation pattern during BMSC osteogenic differentiation.We first identified that the RNA-binding protein human antigen R(HuR)is responsible for HOTAIR nucleocytoplasmic translocation.HOTAIR is essential for osteoblast function,and cytoplasmic HOTAIR binds to miR-214 and acts as a ceRNA to increase Atf4 protein levels and osteoblast function.BglapHOTAIR mice,but not Prx1-HOTAIR mice,showed alleviation of bone loss induced by unloading.This study reveals the importance of temporal and spatial regulation of HOTAIR in BMSC osteogenic differentiation and bone formation,which provides new insights into precise regulation as a target for bone loss. | Yuheng Li Weijia Sun Jianwei Li Ruikai Du Wenjuan Xing Xinxin Yuan Guohui Zhong Dingsheng Zhao Zizhong Liu Xiaoyan Jin Junjie Pan Youyou Li Qi Li Guanghan Kan Xuan Han Shukuan Ling Xiqing Sun Yingxian Li | 2023 | Bone Research2023，11，4： | 0 |
| 9 | Berberine alleviates non-alcoholic hepatic steatosis partially by promoting SIRT1 deacetylation of CPT1A in mice显示文摘Background:Berberine effectively alleviates non-alcoholic fatty liver disease(NAFLD).Nevertheless,the mechanism is incompletely comprehended.It has been reported that SIRT1 mediates lipid metabolism in liver and berberine promotes the expression of SIRT1 in hepatocytes.We hypothesized that SIRT1 mediated the effect of berberine on NAFLD.Methods:The effects of berberine on NAFLD were evaluated in C57BL/6J mice fed a high-fat diet(HFD)and in mouse primary hepatocytes and cell lines exposed to palmitate.The change of fatty acid oxidation(FAO)and the activity of CPT1A were observed in HepG2 cells.Quantitative real-time polymerase chain reaction and Western blot were employed to observe the expression of SIRT1 and lipid metabolism-related molecules.The interaction between SIRT1 and CPT1A was investigated by using co-immunoprecipitation assay in HEK293T cells.Results:Berberine treatment attenuated hepatic steatosis,reduced triglyceride(190.1611.2 lmol/g liver vs 113.667.6 lmol/g liver,P<0.001)and cholesterol(11.362.5 lmol/g liver vs 6.360.4 lmol/g liver,P<0.001)concentration in the liver,and improved lipid and glucose metabolism disorders compared with the HFD group.The expression of SIRT1 was reduced in the liver of NAFLD patients and mouse models.Berberine increased the expression of SIRT1 and promoted the protein level of CPT1A and its activity in HepG2 cells.SIRT1 overexpression mimicked the effect of berberine on reducing triglyceride levels in HepG2 cells,whereas SIRT1 knock-down attenuated the effect of berberine.Mechanistically,berberine increased the expression of SIRT1.SIRT1 deacetylated CPT1A at the Lys675 site,which suppressed its ubiquitin-dependent degradation,thereby promoting FAO and alleviating non-alcoholic liver steatosis.Conclusions:Berberine promoted SIRT1 deacetylation of CPT1A at the Lys675 site,which reduced the ubiquitin-dependent degradation of CPT1A and ameliorated non-alcoholic liver steatosis. | Peng Wang Ruikai Li Yuqi Li Siwei Tan Jie Jiang Huiling Liu Xiuqing Wei | 2023 | Gastroenterology Report2023，11，1： | 0 |
| 10 | Optimization of hot-air microwave combined drying control system based on air outlet temperature and humidity monitoring显示文摘Microwave drying is one of the most important drying methods in agriculture.The online measurement and control of material dried,as a challenging research issue,is helping to improve the drying quality of the final product and the energy utilization efficiency of microwave dryers.In order to realize the online measurement of temperature in microwave drying,a detection strategy was based on the temperature and humidity monitoring at the air outlet of coupling hot air and microwave drying method.LabVIEW programming software was employed to collect the temperature and humidity signal value at the outlet of the dryer to estimate the drying degree of the material.According to the amount of moisture removal in real-time,the microwave input power was adjusted gradually in the microwave drying process.Taking potato as material dried,the microwave power of microwave and hot air coupling drying is monitored to realize the real-time regulation.The results showed that the total color difference value of the product of 3.09,the rehydration ratio of 2.92,the unit energy consumption of 17419.35 kJ/kg,and the drying rate of 0.442 g/s were obtained.The minimum comprehensive weighted score of the product was 26.36.Compared with the orthogonal experiment,the optimal drying process was obtained as the total color difference value of the product of 2.84,the rehydration ratio of 3.01,the unit energy consumption of 17419.35 kJ/kg,and the drying rate of 0.397 g/s.The minimum comprehensive weighted score of the product was 20.67,and the difference was not significant.The control strategy not only solves the non-uniform phenomenon of drying,and makes the microwave drying real-time and continuous,but also improves the drying efficiency and quality. | Fujie Zhang Anqi’er Hu Ruikai Song Lixia Li | 2021 | International Journal of Agricultural and Biological Engineering2021，14，4： | 0 |
| 11 | Single-Photon Lidar for Fast Imaging at Low SBR and Few Photons显示文摘In low-light conditions,the single-photon light detection and ranging(Lidar)technique based on timecorrelated single-photon counting(TCSPC)is suited for collecting a three-dimensional(3D)profile of the target.We present a rapid 3D reconstruction approach for single-photon Lidar with low signal-to-background ratio(SBR)and few photons based on a combination of short-duration range gate selection,photon accumulation of surrounding pixels,and photon efficiency algorithm in this paper.We achieve the best noise filtering and 3D image reconstruction by choosing the optimal combined order of simple methods.Experiments were carried out to validate the various depth estimation algorithms using simulated data and single-photon avalanche diode(SPAD)array data under varying SBR.The experimental results demonstrate that our proposed method can achieve high-quality 3D reconstruction with a faster processing speed compared to the existing algorithms.The proposed technology will encourage the use of single-photon Lidar to suit practical needs such as quick and noise-tolerant 3D imaging. | Xue Ruikai Kang Yan Zhang Tongyi Meng Fanxing Wang Xiaofang Li Weiwei Li Lifei Zhao Wei | 2023 | 激光与光电子学进展2023，60，8： | 0 |
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