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| 1 | Stable classi?cation with limited sample: transferring a 30-m resolution sample set collected in 2015 to mapping 10-m resolution global land cover in 2017显示文摘As the world strives to reduce the impact of population growth, urbanization, agricultural expansion, and climate change on food security, energy and water shortage, resource over-exploration, biodiversity loss, environmental pollution, and ultimately human health, timely and higher resolution land cover information is urgently needed to achieve the sustainable development goals of the United Nations. | Peng Gong Han Liu Meinan Zhang Congcong Li Jie Wang Huabing Huang Nicholas Clinton Luyan Ji Wenyu Li Yuqi Bai Bin Chen Bing Xu Zhiliang Zhu Cui Yuan Hoi Ping Suen Jing Guo Nan Xu Weijia Li Yuanyuan Zhao Jun Yang Chaoqing Yu Xi Wang Haohuan Fu Le Yu Iryna Dronova Fengming Hui Xiao Cheng Xueli Shi Fengjin Xiao Qiufeng Liu Lianchun Song | 2019 | Science Bulletin2019，64，6： | 166 |
| 2 | Qi-Qin Accretionary Belt in Central China Orogen: accretion by trench jam of oceanic plateau and formation of intra-oceanic arc in the Early Paleozoic Qin-Qi-Kun Ocean显示文摘Most orogenic belts have experienced a complex accretionary process with multiple episodes of sea?oor subduction and trench retreat.This accretionary process is important in continenta development and growth[1,2].Three giant orogens extend in China,e.g.,the Central Asian Orogen in the north,the Central China Orogen in the middle and the Himalayan Orogen in the southwest.They are keys for the formation of the Eurasian continent(Fig.1a).The Central China Orogen | Shuguang Song Liming Yang Yuqi Zhang Yaoling Niu Chao Wang Li Su Yanlin Gao | 2017 | Science Bulletin2017，62，15： | 28 |
| 3 | The first all-season sample set for mapping global land cover with Landsat-8 data显示文摘We report the world's first all-season training and validation sample sets for global land cover classification with Landsat-8 data.Prior to this,such samples were only available at a single date primarily from the growing season.It is unknown how much limitation such a single-date sample has to mapping global land cover in other seasons of the year.To answer this question,we selected available Landsat-8 images from four seasons and collected training and validation samples from them.We compared the performances of training samples in different seasons using Random Forest algorithm.We found that the use of training samples from any individual season would result in the best overall classification accuracy when validated by samples in the same season.The global overall accuracy from combined best seasonal results was 67.2% when classifying the 11 Level-1 classes in the Finer Resolution Observation and Monitoring of Global Land Cover(FROM-GLC) classification system.The use of training samples from all seasons(named all-season training sample set hereafter) produced an overall accuracy of 67.0%.We also tested classification within 10° latitude 60° longitude zones using all-season training subsample within each zone and obtained an overall accuracy of 70.2%.This indicates that properly grouped subsamples in space can help improve classification accuracies.All the results in this study seem to suggest that it is possible to use an all-season training sample set to reach global optimality with universal applicability in classifying images acquired at any time of a year for global land cover mapping. | Congcong Li Peng Gong Jie Wang Zhiliang Zhu Gregory S. Biging Cui Yuan Tengyun Hu Haiying Zhang Qi Wang Xuecao Li Xiaoxuan Liu Yidi Xu Jing Guo Caixia Liu Kwame O. Hackman Meinan Zhang Yuqi Cheng Le Yu Jun Yang Huabing Huang Nicholas Clinton | 2017 | Science Bulletin2017，62，7： | 21 |
| 4 | Identification of mecciRNAs and their roles in the mitochondrial entry of proteins显示文摘Mammalian mitochondria have small genomes encoding very limited numbers of proteins.Over one thousand proteins and noncoding RNAs encoded by the nuclear genome must be imported from the cytosol into the mitochondria.Here,we report the identification of hundreds of circular RNAs(mecciRNAs)encoded by the mitochondrial genome.We provide both in vitro and in vivo evidence to show that mecciRNAs facilitate the mitochondrial entry of nuclear-encoded proteins by serving as molecular chaperones in the folding of imported proteins.Known components involved in mitochondrial protein and RNA importation,such as TOM40 and PNPASE,interact with mecciRNAs and regulate protein entry.The expression of mecciRNAs is regulated,and these transcripts are critical for the adaption of mitochondria to physiological conditions and diseases such as stresses and cancers by modulating mitochondrial protein importation.mecciRNAs and their associated physiological roles add categories and functions to the known eukaryotic circular RNAs and shed novel light on the communication between mitochondria and the nucleus. | Xu Liu Xiaolin Wang Jingxin Li Shanshan Hu Yuqi Deng Hao Yin Xichen Bao Qiangfeng Cliff Zhang Geng Wang Baolong Wang Qinghua Shi Ge Shan | 2020 | Science China(Life Sciences)2020，63，10： | 15 |
| 5 | Comprehensive assessment for removing multiple pollutants by plants in bioretention systems显示文摘Bioretention is a best management practice which uses vegetation to improve the pollutant removal rates in the rain water management.To select the best plant species to remove multiple pollutants in a bioretention system,we tested thirty species of plants in a laboratory in Beijing,China.We found that the ability of the plants to reduce concentrations of heavy metals including cadmium(Cd),lead(Pb),zinc(Zn),and ammonium(NH4?–N)is more than90%.The removal efficiencies for NH4?–N,nitrate(NO3-–N),total phosphorus,and copper(Cu)varied markedly among plant species.The single overall best plant was not easy to be determined.To select the best plant species,we used a dynamic neural network to establish an assessment index system,assessment criteria,and an assessment model that is used here for the first time for multiple pollutants’removal.Applying the theory and model,we discovered that Plantago asiatica L.and Digitaria sanguinalis(L.)Scop.are the overall best plants for removing the seven typicalpollutants.This paper will provide a simple and useful guide for the comprehensive assessment of multiple pollutant removal by plants in complex ecological systems. | Xiaohua Yang Ying Mei Jun He Rong Jiang Yuqi Li Jianqiang Li | 2014 | Chinese Science Bulletin2014，59，13： | 14 |
| 6 | Annual 30-m land use/land cover maps of China for 1980–2015 from the integration of AVHRR, MODIS and Landsat data using the BFAST algorithm显示文摘Annual land use land cover(LULC)change information at medium spatial resolution(i.e.at 30 m)is required in numerous subjects,such as biophysical modelling,land management and global change studies.Annual LULC information,however,is usually not available at continental or national scale due to reasons such as insufficient remote sensing data coverage or lack of computational capabilities.Here we integrate high temporal resolution and coarse spatial resolution satellite images(i.e.,Moderate Resolution Imaging Spectroradiometer(MODIS)and Global Inventory Modelling and Mapping Studies(GIMMS)normalized difference vegetation index(NDVI))with high spatial resolution datasets(China’s Land-Use/cover Datasets(CLUDs)derived from 30-meter Landsat TM/ETM+/OLI)to generate reliable annual nominal 30 m LULC maps for the whole of China between 1980 and 2015.We also test the performance of a statistical based change detection algorithm(Breaks for Additive Seasonal and Trend),originally designed for tracking forest change,in classifying all-type LULC change.As a result,a nominal 30 m annual land use/land cover datasets(CLUD-A)from 1980 to 2015 was developed for the whole China.The mapping results were assessed with a change sample dataset,a regional annual validation sample set and a three-year China sample set.Of the detected change years,75.61%matched the exact time of conversion within±1 year.Annual mapping results provided a detail process of urbanization,deforestation,afforestation,water and cropland dynamics over the past 36 years.The consistent characterization of land change dynamics for China can be further used in scientific research and to support land management for policy-makers. | Yidi XU Le YU Dailiang PENG Jiyao ZHAO Yuqi CHENG Xiaoxuan LIU Wei LI Ran MENG Xinliang XU Peng GONG | 2020 | Science China Earth Sciences2020，63，9： | 12 |
| 7 | Outline of Uranium Resources Characteristics and Metallogenetic Regularity in China显示文摘Uranium,as one of twenty-six kinds of important minerals in China,is strategic resource and energy mineral,which has been thoroughly investigated in the project of National Potential Evaluation of Uranium Resource.During the implementation of this project,the authors summarized the metallogenic regularity of uranium resource in China systematically,through the researches of geological characteristics of uranium resource,uranium deposits type(genetic and prediction type),temporal and spatial distribution,and metallogenic series.Based on the investigation of present situation and progress in uranium exploration,this paper proposes the uranium deposits in China should be divided into 4 classes,9 types,21 subtypes in genetic,and 50 types in prediction;suggests to divide China into 29 uranium metallogenic belts and 20 uranium prospective area,and constructs 20uranium-polymetallic metallogenic series,through summarizing temporal and spatial distribution characteristics and metallogenic regularity of uranium deposits in China.The above research is beneficial to the comprehensive understanding of regional uranium metallogenic regularity,and will direct the uranium exploration in the future. | CAI Yuqi ZHANG Jindai LI Ziying GUO Qingyin SONG Jiye FAN Honghai LIU Wusheng QI Fucheng ZHANG Minglin | 2015 | Acta Geologica Sinica(English Edition)2015，89，3： | 11 |
| 8 | Electrospun Nanofibrous Materials for Wound Healing显示文摘Wound dressing materials which are capable of meeting the demands of accelerating wound closure and promoting wound healing process have being highly desired.Electrospun nanofibrous materials show great application potentials for wound healing owing to relatively large surface area,better mimicry of native extracellular matrix,adjustable waterproofness and breathability,and programmable drug delivery process.In this review article,we begin with a discussion of wound healing process and current commercial wound dressing materials.Then,we emphasize on electrospun nanofibrous materials for wound dressing,covering the efforts for controlling fiber alignment and morphology,constructing 3D scaffolds,developing waterproof-breathable membrane,governing drug delivery performance,and regulating stem cell behavior.Finally,we finish with challenges and future prospects of electrospun nanofibrous materials for wound dressings. | Yuping Dong Yuqi Zheng Keyan Zhang Yueming Yao Lihuan Wang Xiaoran Li Jianyong Yu Bin Ding | 2020 | Advanced Fiber Materials2020，2，4： | 10 |
| 9 | Regional accessibility of land traffic network in the Yangtze River Delta显示文摘在一个给定的区域,任何点的可接近性应该是综合地内部、外部的可接近性的评估。用 MapX 部件和 Delphi,作者介绍一个信息系统根据最短的旅行时间计算并且分析地区性的可接近性,这样产生传播图的一个标记。基于陆地交通网络,这份报纸估计礼品和未来在长江三角洲的十六个主要城市的地区性的可接近性。结果证明长江三角洲的地区性的可接近性作为它的核心与上海介绍一把扇子。二个很可存取的城市是的顶上海和 Jiaxing,和底部二是 Taizhou (浙江省) 和 Nantong。与 Sutong 桥牌, Hangzhouwan 桥和 Zhoushan 桥牌的构造,所有城市的地区性的内部可接近性将被改进。特别为 Shaoxing, Ningbo 和 Taizhou (江苏省) ,地区性的内部可接近性将到一个小时被减少,并且另外的城市将平均到大约 25 分钟被弄短。作为在 Nantong 的 Yangkou 港口的构造,在江苏省的港口城市的地区性的外部可接近性将到大约一个小时被加快。 | ZHANG Li LU Yuqi | 2007 | Journal of Geographical Sciences2007，17，3： | 9 |
| 10 | Comparative study on skin temperature response to menstruation at acupuncture points in healthy volunteers and primary dysmenorrhea patients显示文摘OBJECTIVE:To assess skin temperature response to menstruation at acupuncture points in primary dysmenorrhea(PD) patients and healthy volunteers so as to explore acupuncture point specificity in reflecting diseases in the light of skin temperature.METHODS:Fifty-two PD patients and 49 healthy volunteers were recruited.Skin temperature measurements were performed with a skin temperature assessment device at 10 points.Absolute difference between skin temperature of the same point on the left and right side is used as main outcome measure.RESULTS:On the first day of menstruation, when menstrual pain attacking in PD patients, a significant increase in skin temperature difference was detected at Taixi(KI 3) compared with the healthy group(P < 0.01).A significant reduction in skin temperature difference was detected at Taixi(KI 3) in the first day of menstruation compared with those values in the third day after menstruation(P < 0.01)in the healthy group.On the third day after menstruation, a significant reduction in skin temperature difference was found at Zhongdu(LR 6) in PD group compared with the healthy group(P < 0.05).No significant differences of skin temperature were detected at other points(P > 0.05).CONCLUSION:The skin temperature difference at menstruation-relevant points in PD patients did not all change significantly more than those in women without PD.Significant difference was only found in Taixi(KI 3), the Yuan-source point of Kidney meridian. | She Yanfen Ma Liangxiao Zhu Jiang Qi Conghui Wang Yanxia Tang Ling Li Chunhua Yuan Hongwen Liu Yuqi Song Jiashan | 2017 | Journal of Traditional Chinese Medicine2017，37，2： | 8 |
| 11 | Determination of rare earth elements in plant protoplasts by MAA显示文摘A preliminary study on the speciation of rare earth elements in plant cells has been carried out by molecular activation analysis (MAA). Mesophyll protoplasts of Brassica napus were isolated by enzymatic digestion. After being washed with isosmotic solution containing EDTA for several times, the protoplasts were purified by gradient centrifugation. Then the concentration of rare earth elements (REEs) in the protoplasts was determined by neutron activation analysis. The result shows that REEs can enter the cells of the plant. | ZHANG Zhiyong WANG Yuqi SUN Jingxin LI Fuliang CHAI Zhifang XU Lei LI Xin CAO Guoyin | 2000 | Chinese Science Bulletin2000，45，16： | 8 |
| 12 | Altered expression of mitochondrial related genes in the native Tibetan placents by mitochondrial cDNA array analysis显示文摘Objective: To explore the mechanism of native Tibetan fetuses adaptation to hypoxia, we tried to find the different expression genes about mitochondrial function in the native Tibetan placents. Methods: In this study, the placents of native Tibetan and the high-altitude Han (ha-Han) were collected. After the total RNA extraction, the finally synthesized cDNAs were hybridized to mitochondrial array to find the altered expression genes between them. Then, the cytochrome c oxidase 17 (Cox17), dynactin 2 (DCTN2, also known as p50), and vascular endothelial growth factor receptor (VEGFR, also known as KDR) were chosen from the altered expression genes to further verify the array results using the SYBR Green real-time PCR. Because the altered expression genes (such as Cybb and Cox17) in the array results related to the activities of COXI and COXIV, the placental mitochondria activities of COXI and COXIV were measured to find their changes in the hypoxia. Results: By a standard of ≥1.5 or ≤0.67, there were 24 different expressed genes between the native Tibetan and the ha-Han placents, including 3 up-regulated genes and 21 down-regulated genes. These genes were related to energy metabolism, signal transduction, cell proliferation, electron transport, cell adhesion, nucleotide-excision repair. The array results of Cox17, DCTN2 and KDR were further verified by the real-time RT-PCR. Through the mitochondria respiration measurements, the activity of COXI in the native Tibetan placents were higher than that of ha-Han, there was no difference in COXIV activity between them. Conclusion: The altered mitochondrial related genes in the native Tibetan placents may have a role in the high altitude adaptation for fetuses through changing the activity of mitochondrial COX. | Luo Yongjun Gao Wenxiang Zhao Xiuxin SUO Lang Chen Li Liu Fuyu Song Tonglin Chen Jian Gao Yuqi | 2009 | Journal of Medical Colleges of PLA(China)2009，24，1： | 7 |
| 13 | Diamond Raman laser:a promising high-beam-quality and low-thermal-effect laser显示文摘Stimulated Raman-scattering-based lasers provide an effective way to achieve wavelength conversion.However,thermally induced beam degradation is a notorious obstacle to power scaling and it also limits the applicable range where high output beam quality is needed.Considerable research efforts have been devoted to developing Raman materials,with diamond being a promising candidate to acquire wavelength-versatile,high-power,and high-quality output beam owing to its excellent thermal properties,high Raman gain coefficient,and wide transmission range.The diamond Raman resonator is usually designed as an external-cavity pumped structure,which can easily eliminate the negative thermal effects of intracavity laser crystals.Diamond Raman converters also provide an approach to improve the beam quality owing to the Raman cleanup effect.This review outlines the research status of diamond Raman lasers,including beam quality optimization,Raman conversion,thermal effects,and prospects for future development directions. | Yulan Li Jie Ding Zhenxu Bai Xuezong Yang Yuqi Li Jingling Tang Yu Zhang Yaoyao Qi Yulei Wang Zhiwei Lu | 2021 | High Power Laser Science and Engineering2021，9，3： | 7 |
| 14 | STING signaling activation inhibits HBV replication and attenuates the severity of liver injury and HBV-induced fibrosis显示文摘The covalently closed circular DNA(cccDNA)of HBV plays a crucial role in viral persistence and is also a risk factor for developing HBV-induced diseases,including liver fibrosis.Stimulator of interferon genes(STING),a master regulator of DNA-mediated innate immune activation,is a potential therapeutic target for viral infection and virus-related diseases.In this study,agonist-induced STING signaling activation in macrophages was revealed to inhibit cccDNA-mediated transcription and HBV replication via epigenetic modification in hepatocytes.Notably,STING activation could efficiently attenuate the severity of liver injury and fibrosis in a chronic recombinant cccDNA(rcccDNA)mouse model,which is a proven suitable research platform for HBV-induced fibrosis.Mechanistically,STING-activated autophagic flux could suppress macrophage inflammasome activation,leading to the amelioration of liver injury and HBV-induced fibrosis.Overall,the activation of STING signaling could inhibit HBV replication through epigenetic suppression of cccDNA and alleviate HBV-induced liver fibrosis through the suppression of macrophage inflammasome activation by activating autophagic flux in a chronic HBV mouse model.This study suggests that targeting the STING signaling pathway may be an important therapeutic strategy to protect against persistent HBV replication and HBV-induced fibrosis. | Yuqi Li Minjing He Ziyu Wang Zhiyun Duan Zhiwei Guo Ziteng Wang Ruijie Gong Tianhao Chu Jiabin Cai Bo Gao | 2022 | Cellular & Molecular Immunology2022，19，1： | 7 |
| 15 | Changes and Effecting Factors of Grain Production in China显示文摘Based on the data from the Cost-benefit Data of Farm Produce and the China Agricultural Yearbook,this paper aims to examine the spatial and temporal change characteristics of total grain production and its affecting factors.The results show that:1) During 1980 to 2007,total grain production increased from 3.20 × 108 t to 5.02 × 108 t in China,with annual increasing rate of 1.68%.From the regional disparities,most of the regions present increasing trend of total grain production except for several regions with higher level of economic development;2) Grain sown area decreased from 1.17 × 108 ha in 1980 to 1.06 × 108 ha in 2007,which has negative effect on total grain production;3) The increase of grain yield per unit area caused by land use intensity changes contributed to the increase of total grain yield greatly.However,as the land use intensity showed that farmers pay more attention to labor-saving input but not yield-increasing input,the less enthusiasm of farmers in grain production may become an important constraint on fu-ture grain production increase in China;4) Based on the results,this paper proposed different land management poli-cies in different regions,for example,the government should protect cultivated land,promote large scale production.As to the less developed regions,the government should pay more attention to agricultural subsidies to promote farm-ers' enthusiasm in grain production. | CHEN Yuqi LI Xiubin WANG Jing | 2011 | Chinese Geographical Science2011，21，6： | 6 |
| 16 | Removal of PCDD/Fs and PCBs from flue gas using a pilot gas cleaning system显示文摘A 100 Nm3 /hr capacity pilot scale dual bag filter (DBF) system was tested on the flue gas from an actual hazardous waste incinerator (HWI), the removal efficiency of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans (PCDD/Fs) and polychlorinated biphenyls (PCBs) was also studied. The first filter collected most of the fly ash and associated chlorinated organic; then activated carbon (AC) was injected and used to collect phase chlorinated organic from the gas. Concentrations of PCDD/Fs and PCBs after the DBF system were 0.07 and 0.01 ng TEQ/Nm3 , respectively, which were both far below the national emission standard. Comparing with the original single bag filter system, the PCDD/Fs concentration dropped a lot from 0.36 to 0.07 ng TEQ/Nm3 . Increasing AC feeding rate enhanced their collection efficiency, yet reduced the AC utilization efficiency, and it still needs further study to select an appropriate feeding rate in the system. These results will be useful for industrial application and assist in controlling emissions of PCDD/Fs and other persistent organic pollutions from stationary sources in China. | Xiaoqing Lin Yuqi Jin Hailong Wu Tong Chen Xiaodong Li Shengyong Lu Xuguang Jiang Jianhua Yan | 2013 | Journal of Environmental Sciences2013，25，9： | 5 |
| 17 | Finite element analysis of shape control for continuous hot rolling mills of super wide strip steel显示文摘The efficacy of shape control is the core of this technology and the main basis of automatic shape control system model designing.This passage constructs the three-dimensional elastic deformation model of CVC plus roll system in 2250 mm hot rolling mill.Comparing and analyzing different influence of working factors on control characteristic,the shape control characteristic of CVC plus roll system in its whole work time is studied,and the cause is analyzed and the difference of the roll gap curve and crown adjustable area in early and latter work time is compared.The outcome has crucial meaning in both theory and production. | YANG Guanghui LI Yuqi GAO Yang | 2013 | Computer Aided Drafting,Design and Manufacturing2013，23，1： | 5 |
| 18 | Measurement of overburden failure zones in close-multiple coal seams mining显示文摘In the Kaiping Coal field,mining of five coal seams,located within 80 m in the Kailuan Group,#5,#7,#8,#9 and#12 coal seam,is difficult due to small interburden thickness,concentrated stress distributions,high coal seam metamorphism,and complex geological conditions.By using the ZTR12 geological penetration radar(GPR)survey combined with borehole observations,the overburden caving due to mining of the five coals seams was measured.The development characteristics of full-cover rock fractures after mining were obtained from the GPR scan,which provides a measurement basis for the control of rock strata in close multiple coal seam mining.For the first time,it was found that the overburden caving pattern shows a periodic triangular caved characteristic.Furthermore,it is proposed that an upright triangular collapsed pile masonry and an inverted triangular with larger fragments piled up alternately appear in the lower gob.The research results show that the roof structure formed in the gob area can support the key overlying strata,which is beneficial to ensure the integrity and stability of the upper coal seams in multiple-seam mining of close coal seams. | Yang Li Yuqi Ren Syd S.Peng Haozhou Cheng Nan Wang Junbo Luo | 2021 | International Journal of Mining Science and Technology2021，31，1： | 5 |
| 19 | Solvent-free nanocasting toward universal synthesis of ordered mesoporous transition metal sulfide@N-doped carbon composites for electrochemical applications显示文摘Transition metal sulfides (TMSs) have a wide range of applications owing to their intriguing properties.Significant efforts have been devoted to nanostructuring TMSs to enhance their properties and performance,still there is a high need in general synthesis of TMS nanostructures.Herein,for the first time,a simple solvent free reactive nanocasting approach that integrates solid precursor loading,in-situ sulfuration and carbonization into a single heating step is developed for the universal synthesis of ordered mesoporous TMS@N-doped carbon composites (denoted as OM-TMS@NCs) with methionine (Met) and metal chlorides as the precursors and the mesoporous silica (SBA-15) as the hard template.A series of OM-TMS@NCs with a hexagonal mesostructure,ultra-high surface areas (430-754 m2·g-1),large pore volumes (0.85-1.32 cm3·g-1),and unique TMS stoichiometries,including MoS2,Fe7S8,Co9S8,NiS,Cu7S4 and ZnS,are obtained.Two distinct structure configurations,namely,highly dispersed ultrathin TMS nanosheets within NCs and TMS@NC co-nanowire arrays,can be obtained depending on different metals.The structure evolution of the OM-TMS@NCs over the solvent-free nanocasting process is studied in detail for a deep understanding of the synthesis.As demonstrations,these materials are promising for electrocatalytic hydrogen evolution reaction and lithium ion storage with high performances. | Jiahui Zhu Zhi Chen Lin Jia Yuqi Lu Xiangru Wei Xiaoning Wang Winston Duo Wu Na Han Yanguang Li Zhangxiong Wu | 2019 | Nano Research2019，12，9： | 5 |
| 20 | Regulation of phenylpropanoid biosynthesis by MdMYB88 and MdMYB124 contributes to pathogen and drought resistance in apple显示文摘MdMYB88 and MdMYB124 have been demonstrated to be responsible for lignin accumulation in apple under drought stress.In this study,using a metabolomic approach,we identified differentially accumulated phenylpropanoid and flavonoid metabolites in MdMYB88/124 transgenic RNAi plants under control and long-term drought stress conditions in apple roots.We confirmed the regulation of phenylalanine by MdMYB88 and MdMYB124 via UPLC-MS in apple roots under both control and drought conditions.Using Electrophoretic Mobility Shift Assay(EMSA)and ChIPquantitative PCR(qPCR)analyses,we found that MdMYB88 positively regulates the MdCM2 gene,which is responsible for phenylalanine biosynthesis,through binding to its promoter region.Under long-term drought conditions,MdMYB88/124 RNAi plants consistently accumulated increased amounts of H2O2 and MDA,while MdMYB88 and MdMYB124 overexpression plants accumulated decreased amounts of H2O2 and MDA.We also examined the accumulation of metabolites in the phenylpropanoid biosynthesis pathway in the leaves of MdMYB88 and MdMYB124 transgenic apple plants after long-term drought stress.We found that metabolites responsible for plant defense,including phenylpropanoids and flavonoids,accumulated less in the RNAi plants but more in the overexpression plants under both control and drought conditions.We further demonstrated that MdMYB88/124 RNAi plants were more sensitive to Alternaria alternata f.sp.mali and Valsa mali,two pathogens that currently severely threaten apple production.In contrast,MdMYB88 and MdMYB124 overexpression plants were more tolerant to these pathogens.The cumulative results of this study provided evidence for secondary metabolite regulation by MdMYB88 and MdMYB124,further explained the molecular roles of MdMYB88 and MdMYB124 in drought resistance,and provided information concerning molecular aspects of their roles in disease resistance. | Dali Geng Xiaoxia Shen Yinpeng Xie Yusen Yang Ruiling Bian Yuqi Gao Pengmin Li Liying Sun Hao Feng Fengwang Ma Qingmei Guan | 2020 | Horticulture Research2020，7，1： | 5 |
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