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| 1 | DK crush technique: modified treatment of bifurcation lesions in coronary artery显示文摘 | CHEN Shao-liang YE Fei ZHANG Jun-jie ZHU Zhong-sheng LIN Song SHAN Shou-jie LIU Zhi-zhong LIU Yan DUAN Bao-xiang GE Jun-bo | 2005 | Chinese Medical Journal2005，，20： | 35 |
| 2 | Karyotype Stability and Unbiased Fractionation in the Paleo-Allotetraploid Cucurbita Genomes显示文摘 | Honghe Sun Shan WU Guoyu Zhang Chen Jiao Shaogui Guo Yi Ren Jie Zhang Haiying Zhang Guoyi Gong Zhangcai Jia Fan Zhang Jiaxing Tian William J. Lucas Jeff J. Doyle Haizhen Li Zhangjun Fei Yong Xu | 2017 | Molecular Plant2017，10，10： | 33 |
| 3 | Special Section:SARS-CoV-2 Preliminary evidence from a multicenter prospective observational study of the safety and efficacy of chloroquine for the treatment of COVID-19显示文摘Effective therapies are urgently needed for the SARS-CoV-2 pandemic.Chloroquine has been proved to have antiviral effect against coronavirus in vitro.In this study,we aimed to assess the efficacy and safety of chloroquine with different doses in COVID-19.In this multicenter prospective observational study,we enrolled patients older than 18 years old with confirmed SARS-CoV-2 infection excluding critical cases from 12 hospitals in Guangdong and Hubei Provinces.Eligible patients received chloroquinephosphate s00 mg,orally,once(half dose)or twice(full dose)daily.Patients treated with non-chloroquine therapy were included as historical controls.The primary endpoint is the time to undetectable viral RNA.Secondary outcomes include the proportion of patients with undetectable viral RNA by day 10 and 14,hospitalization time,duration of fever,and adverse events.A total of 197 patients completed chloroquine treatment,and 176patients were included as historical controls.The median time to achieve an undetectable viral RNA was shorter in chloroquine than in non-chloroquine(absolute difference in medians-6.0 days;95%CI-6.0 to—4.0).The duration of fever is shorter in chloroquine(geometric mean ratio 0.6;95%CIO.5 to 0.8).No serious adverse events were observed in the chloroquine group.Patients treated with half dose experienced lower rate of adverse events than with full dose.Although randomized trials are needed for further evaluation,this study provides evidence for safety and efficacy of chloroquine in COVID-19 and suggests that chloroquine can be a cost-effective therapy for combating the COVID-19 pandemic. | Mingxing Huang Man Li Fei Xiao Pengfei Pang Jiabi Liang Tiantian Tang Shaoxuan Liu Binghui Chen Jingxian Shu Yingying You Yang Li Meiwen Tang Jianhui Zhou Guanmin Jiang Jingfen Xiang Wenxin Hong Songmei He Zhaoqin Wang Jianhua Feng Changqing Lin Yinong Ye Zhilong Wu Yaocai Li Bei Zhong Ruilin Sun Zhongsi Hong Jing Liu Huili Chen Xiaohua Wang Zhonghe Li Duanqing Pei Lin Tian Jinyu Xia Shanping Jiang Nanshan Zhong Hong Shan | 2020 | National Science Review2020，7，9： | 20 |
| 4 | Depth of tumor invasion and tumor-occupied portions of stomach are predictive factors of intra-abdominal metastasis显示文摘Objective:Diagnostic laparoscopy is recommended for the pretherapeutic staging of gastric cancer to detect any unexpected or unconfirmed intra-abdominal metastasis.The aim of this study was to evaluate the role and indications of diagnostic laparoscopy in the detection of intra-abdominal metastasis.Methods:Standard diagnostic laparoscopy with peritoneal cytology examination was performed prospectively on patients who were clinically diagnosed with primary local advanced gastric cancer(c T≥2M0).We calculated the rate of intra-abdominal metastases identified by diagnostic laparoscopy,and examined the relationship between peritoneal dissemination(P)and cytology results(CY).Split-sample method was applied to find clinical risk factors for intra-abdominal metastasis.Multivariate logistic regression analysis and receiver-operator characteristic(ROC)analysis were performed in training set to find out risk factors of intra-abdominal metastasis,and then validate it in testing set.Results:Out of 249 c M0 patients,51(20.5%)patients with intra-abdominal metastasis were identified by diagnostic laparoscopy,including 20(8.0%)P1CY1,17(6.8%)P0CY1 and 14(5.6%)P1CY0 patients.In the training set,multivariate logistic regression analysis and ROC analysis showed that the depth of tumor invasion on computer tomography(CT)scan≥21 mm and tumor-occupied≥2 portions of stomach are predictive factors of metastasis.In the testing set,when diagnostic laparoscopy was performed on patients who had one or two of these risk factors,the sensitivity and positive predictive value for detecting intra-abdominal metastasis were 90.0%and32.1%,respectively.Conclusions:According to our results,depth of tumor invasion and tumor-occupied portions of stomach are predictive factors of intra-abdominal metastasis. | Ziyu Li Zhemin Li Shuqin Jia Zhaode Bu Lianhai Zhang Xiaojiang Wu Shuangxi Li Fei Shan Xin Ji Jiafu Ji | 2017 | Chinese Journal of Cancer Research2017，29，2： | 14 |
| 5 | Genotypic Characterization of Methicillin-resistant Staphylococcus aureus Isolated from Pigs and Retail Foods in China显示文摘Objective To investigate the genotypic diversity of Methicillin-resistant Staphylococcus aureus(MRSA) isolated from pigs and retail foods from different geographical areas in China and further to study the routes and rates of transmission of this pathogen from animals to food. Methods Seventy-one MRSA isolates were obtained from pigs and retail foods and then characterized by multi-locus sequencing typing(MLST), spa typing, multiple-locus variable number of tandem repeat analysis(MLVA), pulsed-field gel electrophoresis(PFGE), and antimicrobial susceptibility testing. Results All isolated MRSA exhibited multi-drug resistance(MDR). Greater diversity was found in food-associated MRSA(7 STs, 8 spa types, and 10 MLVA patterns) compared to pig-associated MRSA(3 STs, 1 spa type, and 6 MLVA patterns). PFGE patterns were more diverse for pig-associated MRSA than those of food-associated isolates(40 vs. 11 pulse types). Among the pig-associated isolates, CC9-ST9-t899-MC2236 was the most prevalent clone(96.4%), and CC9-ST9-t437-MC621(20.0%) was the predominant clone among the food-associated isolates. The CC9-ST9 isolates showed significantly higher antimicrobial resistance than other clones. Interestingly, CC398-ST398-t034 clone was identified from both pig-and food-associated isolates. Of note, some community-and hospital-associated MRSA strains(t030, t172, t1244, and t4549) were also identified as food-associated isolates. Conclusion CC9-ST9-t899-MC2236-MDR was the most predominant clone in pigs, but significant genetic diversity was observed in food-associated MRSA. Our results demonstrate the great need for improved surveillance of MRSA in livestock and food and effective prevention strategies to limit MDR-MRSA infections in China. | WANG Wei LIU Feng ZULQARNAIN Baloch ZHANG Cun Shan MA Ke PENG Zi Xin YAN Shao Fei HU Yu Jie GAN Xin DONG Yin Ping BAI Yao LI Feng Qin YAN Xiao Mei MA Ai Guo XU Jin | 2017 | Biomedical and Environmental Sciences2017，30，8： | 13 |
| 6 | Seroepidemiological Investigation of Lyme Disease and Human Granulocytic Anaplasmosis among People Living in Forest Areas of Eight Provinces in China显示文摘Objective Lyme disease and Human granulocytic anaplasmosis are tick‐borne diseases caused by Borrelia burgdorferi and Anaplasma phagocytophilum respectively. We have investigated infection and co-infection of the two diseases in the population of forest areas of eight provinces in China by measuring seroprevalence of antibodies against B. burgdorferi and A. phagocytophilum. Methods Forest areas in 8 provinces were chosen for investigation using whole sampling and questionnaire survey methods. 3 669 serum samples from people in the forest areas were tested for the presence of antibodies by indirect immunofluorescent assay (IFA). Results Seroprevalence against B. burgdorferi was 3% to 15% and against A. phagocytophilum was 2% to 18% in the study sites in the 8 provinces in China. We also found co-infection of B. burgdorferi and A. phagocytophilum in 7 of the 8 provinces (the exception being the Miyun area in Beijing). The seroprevalence for both B. burgdorferi and A. phagocytophilum was significantly higher among people exposed to ticks than among people who were not exposed to ticks. Conclusion We conclude that both pathogens are endemic in the forest areas in the eight provinces, but the prevalence of B. burgdorferi and A. phagocytophilum differs between the provinces. | HAO Qin GENG Zhen HOU Xue Xia TIAN Zhen YANG Xiu Jun JIANG Wei Jia SHI Yan ZHAN Zhi Fei LI Guo Hua YU De Shan WANG Hua Yong XU Jian Guo WAN Kang Lin | 2013 | Biomedical and Environmental Sciences2013，26，3： | 13 |
| 7 | Status and distribution changes of the endangered Green Peafowl (Pavo muticus) in China over the past three decades (1990s-2017)显示文摘Background: Understanding the changes in population dynamics, including demographics, distribution and threats, is essential for species status assessing. The endangered Green Peafowl(Pavo muticus) has experienced sharp population declines and distribution range diminishing both in China and Southeast Asia. Field population surveys have not been conducted in China since the 1990 s, which hindered conservation planning and decision-making.Methods: With interview and line transects methods, we figured out the population and distribution changes of Green Peafowl across its historical ranges over the past three decades in China during 2014-2017.Results: The Green Peafowl once habituated in 54 counties in China. Nearly 60% of the distribution counties were lost in the past three decades, with the left 22 counties distributed in central, southern and western Yunnan, SW China. Population decrease detected in all distribution areas except for Shuangbai and Xinping county where more than 60% of the total population is located. Only about 30% of the former bird population were recorded with the same interviewing method as 20 years ago. Three birds, 1 carcass, 6 calls and 12 footprints were detected along the 865 km line transects, indic-ating extremely low encounter rate of Green Peafowl in field. Sharp decreases in flock sizes were also detected, from 820 birds per flock in the 1990 s to 3-5 birds at present. Poaching and habitat conversion are two widespread and long-lasting threats, while poisoning caused mortality in the past and hydropower construction affect regional population's survival. Large flocks of 18–27 birds were discovered in the field, which increases our confidence of population recovery of this endangered pheasant in China.Conclusions: Only interviewed bird number and counts based on line transects were presented in this study, without further population estimation due to limitation of the data sets. Although the actual population of this cryptic bird must be underestimated, dramatic population declines and distribution concentrations of the endangered Green Peafowl occurred over the past 30 years in China undoubtedly. | Dejun Kong Fei Wu Pengfei Shan Jianyun Gao Dao Yan Weixiong Luo Xiaojun Yang | 2018 | Avian Research2018，9，2： | 12 |
| 8 | Qingxuan Jiangya Decoction(清眩降压汤) Prevents Blood Pressure Elevation and Ameliorates Vascular Structural Remodeling via Modulating TGF-β 1/Smad Pathway in Spontaneously Hypertensive Rats显示文摘Objective:To elevate the effects of Qingxuan Jiangya Decoction(清眩降压汤,QXJYD)on hypertension and vascular structural remodeling(VSR)in spontaneously hypertensive rats(SHRs),and investigate the underlying mechanisms.Methods:SHRs(n=8)were given intra-gastric administration with 60 mg/kg of QXJYD or saline,daily for 8 weeks,while rats in SHR-control(n=8)and WKY(n=8)groups were received equal volumes of saline solution.Systolic blood pressures(SBP),diastolic blood pressures(DBP)and mean blood pressures(MBP)were measured once a week.The levels of angiotensinⅡ(AngⅡ),endothelin 1(ET-1)and plasma renin activity(PRA)were tested by enzyme-linked immunosorbent assay(ELISA)and radioimmunoassay,respectively.The effect of QXJYD on VSR was determined by examining the media thickness and the ex vivo contractility of thoracic aortic.The proliferation and fibrosis of vascular smooth muscle cells(VSMCs)were examined via immunohistochemical(IHC)staining for proliferating cell nuclear antigen(PCNA),collagen Ⅰ and collagen Ⅲ,respectively.The mRNA and protein expressions of transforming growth factor β1(TGF-β1),Smad3 and phosphorylation of Smad3 in thoracic aorta tissues were determined by real-time polymerase chain reaction(PCR)and Western blot assay,respectively.Results:QXJYD treatment led to a significant decrease of the elevation of blood pressure in SHRs and reduced the levels of AngⅡ,ET-1 and PRA in the serum(P<0.05).In addition,QXJYD treatment remarkably ameliorated VSR and vascular function in SHRs.Moreover,QXJYD inhibited VSMC proliferation and fibrosis by suppressing the expression of PCNA,collagen Ⅰ and collagen Ⅲ in thoracic aortic.Furthermore,QXJYD inhibited the expression of TGF-β1,Smad3 and the phosphorylation of Smad3,respectively(P<0.05).Conclusion:QXJYD reversed VSR by inhibiting VSMC proliferation and collagen deposition via regulation of TGF-β1/Smad signaling pathway,which may,in part,illuminate its anti-hypertensive activities. | HE Fei CHU Jian-feng CHEN Hong-wei LIN Wei LIN Shan CHEN You-qin PENG Jun CHEN Ke-ji | 2020 | Chinese Journal of Integrative Medicine2020，26，3： | 12 |
| 9 | Genome-wide association study dissects the genetic bases of salt tolerance in maize seedlings显示文摘Excess salinity is a natural stress that causes crop yield losses worldwide. The genetic bases of maize salt tolerance remain largely unknown. Here we investigated the survival rates of 445 maize natural accessions after salt treatments. A skewed distribution of the salttolerant phenotypes was observed in this population.Genome-wide association studies(GWAS) revealed 57 loci significantly associated with salt tolerance. Forty-nine candidate genes were detected from these loci. About10% of these genes were co-localized with loci from QTL mapping. Forty four percent of the candidate genes were involved in stress responses, ABA signaling,stomata division, DNA binding/transcription regulation and auxin signaling, suggesting that they are key genetic mechanisms of maize salt tolerance. Transgenic studies showed that two genes, the salt-tolerance-associatedgene 4(SAG4, GRMZM2 G077295) and SAG6(GRMZM2 G106056), which encode a protein transport protein and the double-strand break repair protein MRE11,respectively, had positive roles in plant salt tolerance,and their salt-tolerant haplotypes were revealed. The genes we identified in this study provide a list of candidate targets for further study of maize salt tolerance, and of genetic markers and materials that may be used for breeding salt-tolerance in maize. | Xi Luo Bingcai Wang Shan Gao Fei Zhang William Terzaghi Mingqiu Dai | 2019 | Journal of Integrative Plant Biology2019，61，6： | 8 |
| 10 | The RNA-binding protein QKI5 regulates primary miR-124-1 processing via a distal RNA motif during erythropoiesis显示文摘 | Fang Wang Wei Song Hongmei Zhao Yanni Ma Yuxia Li Di Zhai Jingnan Pi Yanmin Si Jiayue Xu Lei Dong Rui Su Mengmeng Zhang Yong Zhu Xiaoxia Ren Fei Miao Wenjie Liu Feng Li Junwu Zhang Aibin He Ge Shan Jingyi Hui Linfang Wang Jia Yu | 2017 | Cell Research2017，27，3： | 7 |
| 11 | Imbalance of the reciprocally inhibitory loop between the ubiquitin-specific protease USP43 and EGFR/PI3K/AKT drives breast carcinogenesis显示文摘 | Lin He Xinhua Liu Jianguo Yang Wanjin Li Shumeng Liu Xujun Liu Ziran Yang Jie Ren Yue Wang Lin Shan Chengjian Guan Fei Pei Liandi Lei Yu Zhang Xia Yi Xiaohan Yang Jing Liang Rong Liu Luyang Sun Yongfeng Shang | 2018 | Cell Research2018，28，9： | 5 |
| 12 | Anatomical variation of infra-pyloric artery origination: A prospective multicenter observational study (IPA-Origin)显示文摘Objective: Infra-pyloric artery(IPA) is an important anatomical landmark in treatment of gastric cancer and is the key vessel for pylorus-preserving gastrectomy and subgroup of infra-pyloric lymph nodes. However, its anatomical variation is not thoroughly understood. Our study aimed to clarify the origination of the IPA.Methods: We did this prospective, multicenter, open-label, observational study at gastric surgery departments of34 hospitals in China. Gastric cancer patients aged 18 years or older and scheduled to undergo elective total or distal gastrectomy were assigned. During the surgery, IPA dissecting and exposing the origination point with photographs or video clips were required. The primary outcome was the origination of the IPA. Analysis of variance, χ~2 tests and Fisher's tests were used to analyze the differences between groups. The study is registered at Clinicaltrials.gov(No. NCT03071237).Results: Between May 8 and July 31, 2017, 429 patients were assigned for the study, and 419(97.7%) patients had the IPA dissected and recorded through photograph or video and were included in the primary outcome analysis. The median age was 62 years old, and 73.7% were male. Among the patients, 78.5% received laparoscopic surgery. Single IPA origination was identified in 398(95.0%) patients, including gastroduodenal artery(GDA) in154(36.8%) patients, anterior superior pancreaticoduodenal artery(ASPDA) in 130(31.0%) patients, and right gastroepiploic artery(RGEA) in 114(27.2%) patients. Fifteen(3.6%) patients were identified with multiple IPA and 6(1.4%) patients were identified as IPA absence. The differences in the distribution of surgical approach(P=0.003) and geographic area(P=0.030) were statistically significant. No difference was shown in sex, age,gastrectomy type, tumor location, and clinical T, N and M stage.Conclusions: Our study found that the IPA originates from GDA, ASPDA and RGEA in similar proportions.Laparoscopic surgery may be more helpful in dissection of the IPA than open surgery. | Rulin Miao Jianjun Qu Zhengrong Li Daguang Wang Jiang Yu Weidong Zang Yong Li Fenglin Liu Jian Zhang Wu Song Kai Ye Su Yan Wei Wang Shuangyi Ren Lu Zang Changqing Jing Li Zhang Kuan Wang Weihua Fu Lin Fan Bin Liang Gang Zhao Jun Cai Li Yang Jiaming Zhu Jun You Kun Yang Qingxing Huang Zhaojian Niu Ning Ning Xingfeng Qiu Gang Ji Feng Liang Hua Huang Chao Gao Fei Shan Shuangxi Li Yongning Jia Lianhai Zhang Xiangji Ying Yan Zhang Zhaode Bu Xiangqian Su Gang Zhao Ziyu Li Jiafu Ji | 2018 | Chinese Journal of Cancer Research2018，30，5： | 5 |
| 13 | Effect of coronary bifurcation angle on clinical outcomes in Chinese patients treated with crush stenting: a subgroup analysis from DKCRUSH-1 bifurcation study显示文摘背景分叉角度可以影响压碎 stenting 的临床的结果。我们寻求了比较高度(60 ( | CHEN Shao-liang ZHANG Jun-jie YE Fei CHEN Yun-dai FANG Wei-yi WEI Meng HE Ben SUN Xue-wen YANG Song CHEN Jin-guo SHAN Shou-jie TIAN Nai-liang LI Xiao-bo LIU Zhi-zhong KAN Jing Lee Michael Kwan Tak W | 2009 | Chinese Medical Journal2009，，4： | 5 |
| 14 | Shexiang Tongxin Dropping Pill(麝香通心滴丸)Protects against Na2S2O4-Induced Hypoxia-Reoxygenation Injury in H9c2 Cells显示文摘Objectives: To investigate the protective effects of Shexiang Tongxin Dropping Pill(麝香通心滴丸,STP) on Na2S2O4-induced hypoxia-reoxygenation injury in cardiomyoblast H9c2 cells. Methods: The cell viability and levels of mRNA and protein expression in H9c2 cells were determined following Na2S2O4-induced hypoxia using Hoechst staining, annexin V/propidium iodide(PI) flow cytometry, real-time polymerase chain reaction and Western blot analysis. Results: STP pretreatment signi?cantly increased the viability and inhibited aberrant morphological changes in H9c2 cardiomyoblast cells induced by Na2S2O4 treatment(P<0.05). In addition, STP pretreatment attenuated Na2S2O4-induced hypoxic damage, down-regulated the expression of pro-apoptotic Bax,and up-regulated the expression of anti-apoptotic Bcl-2 in H9c2 cells(P<0.05). Conclusions: STP was strongly cardioprotective in hypoxia-reoxygenation injury by preventing hypoxic damage and inhibiting cellular apoptosis.These results further support the use of STP as an effective drug for the treatment of ischemic heart disease. | LIN Shan LIN Jiu-mao ZHANG Ling CHEN Da-xin XIAO Fei CHEN Hong-wei CHEN You-qin ZHU Yu-ling CHU Jian-feng PENG Jun | 2019 | Chinese Journal of Integrative Medicine2019，25，6： | 5 |
| 15 | Imatinib mesylate in clinically suspected gastric stromal tumors显示文摘Gastrointestinal stromal tumors(GISTs)occur most frequently in the stomach.Diagnosis of gastric GIST is not always clear before surgery.Flexible endoscopy may suggest the nature of the lesion(a bulky tumor with preserved mucosa);however,biopsy is rarely diagnostic.Therefore,diagnostic medication with safe drugs may provide a feasible way under such conditions after an informed consent is obtained.Based on the excellent efficacy of imatinib mesylate(IM)in the treatment of GIST,we successfully applied it in the diagnostic medication of two patients with clinically suspected gastric stromal tumors.In conclusion,the diagnostic medication with IM can be an alternative option for patients with suspected GIST that can not be confirmed pathologically. | Zi-Yu Li Lei Tang Shuang-Xi Li Fei Shan Zhao-De Bu Jia-Fu Ji | 2013 | Chinese Journal of Cancer Research2013，25，5： | 4 |
| 16 | Hexafluoro-2-propanol as a potent cosolvent for chemical ligation of membrane proteins显示文摘The study on membrane proteins is an important challenge mainly because of their very poor solubility in various solvents.The traditional recombinant expression strategy and the native chemical ligation method both have difficulty in generating sufficient amounts of desired proteins with high efficiency.Previous studies have shown that multiply fluorinated alcohols exhibit good ability to dissolve difficult peptide sequences,especially hexafluoro-2-propanol (HFIP).In the present study we systematically studied the capability of solvents containing different percentage of HFIP in dissolving transmembrane peptides.Through both HPLC and UV analyses we concluded that 60% HFIP/8 M urea constituted a good solvent system.In this solvent system we also optimized conditions to perform native chemical ligation (NCL).Under the optimized conditions we successfully achieved NCL’s for both dipeptide formation and the synthesis of a model protein (Trifolitoxin).These results suggested that HFIP was a potential cosolvent that could be used in the ligation of poorly soluble peptides for the generation of membrane proteins. | SHEN Fei,TANG Shan & LIU Lei Department of Chemistry,University of Science and Technology of China,Hefei 230026,China Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology,Ministry of Education Department of Chemistry,Tsinghua University.Beijing 100084,China | 2011 | Science China Chemistry2011，54，1： | 4 |
| 17 | A local-optimization refinement algorithm in single particle analysis for macromolecular complex with multiple rigid modules显示文摘ABSTRACTSingle 粒子分析,能从千被认为是信号的一般水准或弄平百万粒子设计,是一个有效方法学习生物大分子的三维的结构。在单个粒子分析的一个内在的假设是所有分析粒子必须有相同作文和符合构造。因此,在作文或符合构造的标本异质为高分辨率的分析提起了大挑战。为有多重符合构造的粒子,不精密的排列和取向参数愿望与减少的分辨率和涂的密度产出一张平均地图。除广泛的分类途径以外,这里在平衡附近基于 macromolecular 建筑群由取向和位置在其亲戚的多重僵硬模块组成的假设轻视变化,我们建议一个新方法作为本地优化精炼打电话到地址为一个改进决定的这 conformational 异质。关键想法是优化每个僵硬模块的取向和移动参数然后重建他们的三维的结构个别地。用有相对变化在之间的 80S/70S 核糖体的模仿的数据大(60S/50S ) 并且小(40S/30S ) 子单元,我们测试了这个算法并且发现两个子单元的分辨率显著地被改进。我们的方法为 macromolecular 建筑群的高分辨率的单个粒子分析向一个 proof-of-principle 答案提供动态符合构造。 | Hong Shan Zihao wang Fa Zhang Yong Xiong Chang-Cheng Yin Fei Sun | 2016 | Protein & Cell2016，7，1： | 4 |
| 18 | The role of MAPK and FAS death receptor pathways in testicular germ cell apoptosis induced by lead显示文摘现在的学习的目的是调查在阴囊的细菌房间的导致铅的 apoptosis 涉及 MAPK 的信号小径和船边交货的死亡信号受体小径的基因表示。首先,房间生存能力被 MTT 试金决定。第二,使用单个房间胶化电气泳动测试(彗星试金) 和染色技术, apoptotic 率和阴囊的细菌房间的房间 apoptosis 本地化的 TUNEL 在分别地与 0.15% , 0.3% ,和 0.6% 铅一起对待的老鼠被测量。第三, K 地岬, c-fos,船边交货,和活跃 caspase-3 蛋白质的 immunolocalization 被 immunohistochemistry 决定。最后,在 K 地岬, c-fos,船边交货,和活跃 caspase-3 的翻译层次的变化被西方的污点分析进一步检测。我们的结果证明铅能显著地以一种剂量依赖者方式导致阴囊的细菌房间 apoptosis (P < 0.01 ) 。机制是仔细与在 apoptotic 细菌房间的 K 地岬, c-fos,船边交货,和活跃 caspase-3 的增加的表情有关。在结论, K-ras/c-fos 和 Fas/caspase-3 死亡发信号受体小径在老鼠涉及阴囊的细菌房间的导致铅的 apoptosis。 | Shuying Dong Duoping Liang Na An Li Jia Yujuan Shan Chao Chen Kuo Sun Fei Niu Huiyan Li Songbin Fu | 2009 | Acta Biochimica et Biophysica Sinica2009，41，9： | 4 |
| 19 | Intraspecific DNA methylation polymorphism in the non-edible oilseed plant castor bean显示文摘Investigation of the relationships of phenotypic and epigenetic variations might be a good way to dissect the genetic or molecular basis of phenotypic variation and plasticity in plants. Castor bean(Ricinus communis L.), an important non-edible oilseed crop, is a mono-species genus plant in the family Euphorbiaceae. Since it displays rich phenotypic variations with low genetic diversity, castor bean is a good model to investigate the molecular basis of phenotypic and epigenetic variations. Cytosine DNA methylation represents a major molecular mechanism of epigenetic occurrence. In this study, epigenetic diversity of sixty landrace accessions collected worldwide was investigated using the methylationsensitive amplification polymorphism(MSAP) technique. Results showed that the epigenetic diversity(based on the polymorphism of DNA methylated loci) exhibited a medium variation(Ne=1.395,He=0.242,I=0.366) at the population level though the variation was great, ranging from 3.80% to 34.31% among accessions. Both population structure analysis and the phylogenetic construction(using the neighbor-joining criteria) revealed that the two main clades were identified, but they did not display a distinct geographic structure. After inspecting the location of polymorphic methylated loci on genome we identified that the polymorphic methylated loci occur widely in nuclear and organelle genomes. This study provides new data to understand phenotypic and epigenetic variations in castor bean. | Shan He Wei Xu Fei Li Yue Wang Aizhong Liu | 2017 | Plant Diversity2017，39，5： | 4 |
| 20 | A 256×256 time-of-flight image sensor based on center-tap demodulation pixel structure显示文摘This paper proposes a 256×256 time-of-flight(TOF) image sensor based on the center-tap(CT)demodulation pixel structure. The image sensor can capture both the two-dimensional(2D) high speed image and the three-dimensional(3D) depth image. The CT pixel consists of two split pinned photodiode(PPD)regions and two pairs of transfer transistors. The transfer transistors adopt a non-uniform doped channel(NUDC) structure, which can increase the electron transfer speed along the transfer channel and eliminate the image lag for high speed imaging. The pixel size is 10 μm×10 μm, and we design the implementation process of the pixel to increase the electron transfer speed. The sensor is fabricated in a 0.18 μm 1P5 M CMOS image sensor process. Test results show that it can capture the 430-fps intensity image and the 90-fps depth image in two different imaging modes. The rectified non-linearity within the 1.0–7.5 m depth measurement range achieves less than 3 cm, and the measurement accuracy achieves 4.0 cm at 2.5 m, corresponding to the relative error of1.6%. | Zhe CHEN Shan DI Zhongxiang CAO Qi QIN Fei JI Liyuan LIU Nanjian WU | 2016 | Science China(Information Sciences)2016，59，4： | 4 |
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