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| 1 | Cloning and functional characterization of Rht8,a“Green Revolution”replacement gene in wheat显示文摘Dear Editor,Wheat(Triticum aestivum)is a major staple crop in the world and provides~20%of the food calories for human consumption(Appels et al.,2018).During the 1960s and 1970s,the introduction of two semi-dwarfing genes Rht-B1b and Rht-D1b in wheat varieties strikingly improved lodging resistance and harvest index,and therefore substantially increased grain yield,resulting in the well-known'Green Revolution'(Peng et al.,1999).However,plants carrying Rht-B1b and Rht-D1b suffer from several drawbacks. | Hongchun Xiong Chunyun Zhou Meiyu Fu Huijun Guo Yongdun Xie Linshu Zhao Jiayu Gu Shirong Zhao Yuping Ding Yuting Li Jiazi Zhang Ke Wang Xuejun Li Luxiang Liu | 2022 | Molecular Plant2022，15，3： | 7 |
| 2 | Rumen microbiome structure and metabolites activity in dairy cows with clinical and subclinical mastitis显示文摘Background:Due to the high prevalence and complex etiology,bovine mastitis(BM)is one of the most important diseases to compromise dairy cow health and milk quality.The shift in milk compositions has been widely investigated during mastitis,but recent studies suggested that gastrointestinal microorganism also has a crucial effect on the inflammation of other peripheral tissues and organs,including the mammary gland.However,research focused on the variation of rumen inner-environment during mastitis is still limited.Therefore,the ruminal microbial profiles,metabolites,and milk compositions in cows with different udder health conditions were compared in the present study.Furthermore,the correlations between udder health status and ruminal conditions were investigated.Based on the somatic cell counts(SCC),California mastitis test(CMT)parameters and clinical symptoms of mastitis,60 lactating Holstein dairy cows with similar body conditions(excepted for the udder health condition)were randomly divided into 3 groups(n=20 per group)including the healthy(H)group,the subclinical mastitis(SM)group and the clinical mastitis(CM)group.Lactation performance and rumen fermentation parameters were recorded.And rumen microbiota and metabolites were also analyzed via 16S rRNA amplicon sequencing and untargeted metabolomics,respectively.Results:As the degree of mastitis increased,rumen lactic acid(LA)(P<0.01),acetate,propionate,butyrate,valerate(P<0.001),and total volatile fatty acids(TVFAs)(P<0.01)concentrations were significantly decreased.In the rumen of CM cows,the significantly increased bacteria related to intestinal and oral inflammation,such as Lachnospiraceae(FDR-adjusted P=0.039),Moraxella(FDR-adjusted P=0.011)and Neisseriaceae(FDR-adjusted P=0.036),etc.,were accompanied by a significant increase in 12-oxo-20-dihydroxy-leukotriene B4(FDR-adjusted P=5.97×10^(−9))and 10beta-hydroxy-6beta-isobutyrylfuranoeremophilane(FDR-adjusted P=3.88×10^(−10)).Meanwhile,in the rumen of SM cows,the Ruminiclostridium_9(FDR-adjusted P=0.042)and Enterorhabdus(FDR-adjusted P=0.043)were increased along with increasing methenamine(FDR-adjusted P=6.95×10^(−6)),5-hydroxymethyl-2-furancarboxaldehyde(5-HMF)(FDR-adjusted P=2.02×10^(−6))and 6-methoxymellein(FDR-adjusted P=2.57×10^(−5)).The short-chain fatty acids(SCFAs)-producing bacteria and probiotics in rumen,including Prevoterotoella_1(FDRadjusted P=0.045)and Bifidobacterium(FDR-adjusted P=0.035),etc.,were significantly reduced,with decreasing 2-phenylbutyric acid(2-PBA)(FDR-adjusted P=4.37×10^(−6)).Conclusion:The results indicated that there was a significant shift in the ruminal microflora and metabolites associated with inflammation and immune responses during CM.Moreover,in the rumen of cows affected by SM,the relative abundance of several opportunistic pathogens and the level of metabolites which could produce antibacterial compounds or had a competitive inhibitory effect were all increased. | Yue Wang Xuemei Nan Yiguang Zhao Linshu Jiang Mengling Wang Hui Wang Fan Zhang Fuguang Xue Dengke Hua Jun Liu Junhu Yao Benhai Xiong | 2021 | Journal of Animal Science and Biotechnology2021，12，3： | 6 |
| 3 | Modified pectin-based carrier for gene delivery:Cellular barriers in gene delivery course显示文摘 | Tali Katav LinShu Liu Tamar Traitel | 2008 | Journal of Controlled Release2008，130，： | 1 |
| 4 | Pectin-based systems for colon-specific drug delivery via oral route显示文摘 | Linshu Liu Marshall L Fishman | 2003 | Biomaterials2003，24，： | 1 |
| 5 | Searching algorithm based on node ability automatically fitting in P2P network 显示文摘 | CHEN Linshu LIU Yuanhui | 2009 | Journal of Hunan University of Science&Technology:Nat- ural Science Edition2009，24，2： | 1 |
| 6 | Gelatin-pectin composite films from polyion-complex hydrogels 显示文摘 | Stefano Farris Karen M Schaich Liu Linshu | 2011 | Food Hydrocolloids2011，25，1： | 1 |
| 7 | Pectin-based systems for colon-specific drug delivery via oral route显示文摘 | Liu Linshu Marshall L Fishman Joseph Kost | 2003 | Biomaterials2003，24，19： | 1 |
| 8 | Reinforcing and toughening effects of bamboo pulp fiber on poly (3-hydroxybutyrate-co-3-hydroxyvaIerate) fiber composites 显示文摘 | Jiang Long Chen Feng Qian Jun Huang Jijun Wolcott M Liu Linshu Zhang Jinwen | 2009 | Industrial & Engineering Chemistry Research2009，49，2： | 1 |
| 9 | The effect of hepatitis B virus infected embryos on pregnancy outcome显示文摘 | Feng Ye Yi Liu Yan Jin Juanzi Shi Xueliang Yang Xiaojing Liu Xi Zhang Shumei Lin Ying Kong Linshu Zhang | 2014 | European Journal of Obstetrics and Gynecology2014，，： | 1 |
| 10 | Development of polyion-complex hydrogels as an alternative approach for the production of bio-based polymers for food packaging applications: a review显示文摘 | Stefano Farris Karen M. Schaich LinShu Liu Luciano Piergiovanni Kit L. Yam | 2009 | Trends in Food Science & Technology2009，，8： | 1 |
| 11 | An osteoconductive collagen/hyaluronate matrix for bone regeneration 显示文摘 | Liu Linshu Thompson AY Heidaran MA | 1999 | Biomaterials1999，20，12： | 1 |
| 12 | Composite films from pectin and fish skin gelatin or soybean flour protein显示文摘 | Liu Linshu Liu Chengkung Marshall L Fishman | 2007 | J Agric Food Chem2007，55，： | 1 |
| 13 | Colon-specific delivery of a probiotic-derived soluble protein ameliorates intestinal inflammation in mice through an EGFR-dependent mechanism显示文摘 | Yan Fang Cao Hanwei Cover Timothy L Washington M Kay Shi Yan Liu LinShu Chaturvedi Rupesh Peek Richard M Wilson Keith T Polk D Brent | 2011 | Journal of Clinical Investigation2011，，6： | 1 |
| 14 | Preparation and properties of water and glycerol-plasticized sugar beet pulp plastics 显示文摘 | Liu Bo Zhang Jinwen Liu Linshu | 2011 | Journal of Polymers and the Environment2011，19，3： | 1 |
| 15 | Dietary supplementation with inulin improves lactation performance and serum lipids by regulating the rumen microbiome and metabolome in dairy cows显示文摘This study investigated the effects of inulin on rumen fermentation parameters,ruminal microbiome and metabolites,as well as lactation performance and serum indexes in dairy cows.Sixteen Holstein dairy cows with similar body conditions were randomly divided into 2 groups(n=8 per group),with inulin addition at 0 and 200 g/d percow.The experiment lasted for 6 weeks,including a 1-week adaptation period and a 5-week treatment period.At the end of the experimental period,the milk,serum and rumen fluid were sampled and analyzed.The microbiome and metabolome in the rumen fluid were analyzed via 16 S rRNA sequencing and untargeted metabolomics,respectively.The results showed that supplementation with inulin(200 g/d per cow)increased the milk yield(P=0.001),milk protein(P=0.032),lacto se rate(P=0.004)and proportion of saturated fatty acids(SFA)in milk(P<0.001),but decreased the proportion of unsaturated fatty acids(USFA)(P=0.041).Rumen pH(P=0.040)and the concentration of NH3-N(P=0.024)were decreased;however,acetate(P<0.001),propionate(P=0.003),butyrate(P<0.001)and lactic acid(LA)(P=0.043)were increased.The total cholesterol(TC)(P=0.008)and triglycerides(TG)(P=0.01)in serum were also reduced.Additionally,inulin addition elevated the relative abundance of several beneficial symbiotic and short-chain fatty acid(SCFA)-producing bacteria,such as Muribaculaceae(false discovery rate[FDR]-adjusted P<0.01),Acetitomaculum(FDR-adjusted P=0.043),and Bu tyrivibrio(FDR-adjusted P=0.036),while elevating the levels of L-lysine(FDR-adjusted P=4.24×10^(-3)),L-proline(FDR-adjusted P=0.0158),and L-phenylalanine(FDRadj usted P=0.027).In contrast,several pathogens and ruminal bacteria abu ndant in high-fat diets,such as Escherichia-Sh igella(FD R-adj usted P=0.022),Erysipelo trichaceaeUCG-004(FD R-adjusted P<0.01)and RF39(FDR-adjusted P=0.042)were decreased along with the reduction of lysophosphatidylcholine(LysoPC)(18:1(9 Z))(FDR-adjusted P=1.03×10^(-3)),LysoPC(16:0)(FDR-adjusted P=0.0108),LysoPC(18:2(9 Z,12 Z))(FDRadjusted P=1.65×10^(-3))and 8-methylnonenoate.In conclusion,dietary inulin supplementation could increase the relative abundance of commensal microbiota and SCFA-producing bacteria,upregulate amino acidmetabolism and downregulate lipid metabolism in the rumen of dairy cows,which might further improve lactation performance and the level of serum lipids. | Yue Wang Xuemei Nan Yiguang Zhao Linshu Jiang Hui Wang Dengke Hua Fan Zhang Yapin Wang Jun Liu Junhu Yao Benhai Xiong | 2021 | Animal Nutrition2021，，4： | 1 |
| 16 | Feeding citrus flavonoid extracts decreases bacterial endotoxin and systemic inflammation and improves immunometabolic status by modulating hindgut microbiome and metabolome in lactating dairy cows显示文摘The objectives of this study were to determine the effects of dietary supplementation with citrus flavonoid extracts(CFE)on milk performance,serum biochemistry parameters,fecal volatile fatty acids,fecal microbial community,and fecal metabolites in dairy cows.Eight multiparous lactating Holstein cows were used in a replicated 4×4 Latin square design(21-day period).Cows were fed a basal diet without addition(CON)or basal diet with added CFE at 50(CFE50),100(CFE10),and 150 g/d(CFE150).Feeding CFE up to 150 g/d increased milk yield and milk lactose percentage.Supplementary CFE linearly decreased milk somatic cell count.Serum cytokines interleukin-1β(IL-1β),IL-2,IL-6,and tumor necrosis factor-α(TNF-α)concentrations decreased linearly as the levels of CFE increased.Cows in CFE150 had lower serum lipopolysaccharide and lipopolysaccharide binding protein compared with CON.These results indicate feeding CFE decreased systemic inflammation and endotoxin levels in dairy cows.Furthermore,feeding CFE linearly increased the concentrations of total volatile fatty acids,acetate,and butyrate in feces.The relative abundances of beneficial bacteria Bifidobacterium spp.,Clostridium coccoides-Eubacterium rectale group,and Faecalibacterium prausnitzii in feces increased linearly with increasing CFE supplementation.The diversity and community structure of fecal microbiota were un-affected by CFE supplementation.However,supplementing CFE reduced the relative abundances of genera Ruminococcus_torques_group,Roseburia,and Lachnospira,but increased genera Bacteroides and Phascolarctobacterium.Metabolomics analysis showed that supplementary CFE resulted in a significant modification in the fecal metabolites profile.Compared with CON,fecal naringenin,hesperetin,hippuric acid,and sphingosine concentrations were greater in CFE150 cows,while fecal GlcCer(d18:1/20:0),Cer(d18:0/24:0),Cer(d18:0/22:0),sphinganine,and deoxycholic acid concentrations were less in CFE150 cows.Predicted pathway analysis suggested that'sphingolipid metabolism'was significantly enriched.Overall,these results indicate that citrus flavonoids could exert health-promoting effects by modulating hindgut microbiome and metabolism in lactating cows. | Yuchao Zhao Shiqiang Yu Liuxue Li Huiying Zhao Yuqin Li Linshu Jiang Ming Liu | 2023 | Animal Nutrition2023，，2： | 0 |
| 17 | Could natural phytochemicals be used to reduce nitrogen excretion and excreta‑derived N_(2)O emissions from ruminants?显示文摘Ruminants play a critical role in our food system by converting plant biomass that humans cannot or choose not to consume into edible high-quality food.However,ruminant excreta is a significant source of nitrous oxide(N_(2)O),a potent greenhouse gas with a long-term global warming potential 298 times that of carbon dioxide.Natural phytochemicals or forages containing phytochemicals have shown the potential to improve the efficiency of nitrogen(N)utilization and decrease N_(2)O emissions from the excreta of ruminants.Dietary inclusion of tannins can shift more of the excreted N to the feces,alter the urinary N composition and consequently reduce N_(2)O emissions from excreta.Essential oils or saponins could inhibit rumen ammonia production and decrease urinary N excretion.In grazed pastures,large amounts of glucosinolates or aucubin can be introduced into pasture soils when animals consume plants rich in these compounds and then excrete them or their metabolites in the urine or feces.If inhibitory compounds are excreted in the urine,they would be directly applied to the urine patch to reduce nitrification and subsequent N_(2)O emissions.The phytochemicals’role in sustainable ruminant production is undeniable,but much uncertainty remains.Inconsistency,transient effects,and adverse effects limit the effectiveness of these phytochemicals for reducing N losses.In this review,we will identify some current phytochemicals found in feed that have the potential to manipulate ruminant N excretion or mitigate N_(2)O production and deliberate the challenges and opportunities associated with using phytochemicals or forages rich in phytochemicals as dietary strategies for reducing N excretion and excreta-derived N_(2)O emissions. | Yuchao Zhao Ming Liu Linshu Jiang Leluo Guan | 2024 | Journal of Animal Science and Biotechnology2024，15，2： | 0 |
| 18 | Effects of different molecular weights of chitosan on methane production and bacterial community structure in vitro显示文摘As a new feed additive,chitosan has been shown in recent years to have a certain role in reducing methane emissions from the gastrointestinal tracts of ruminants.However,the effects of chitosan with different molecular weights on rumen ferme ntation,methane production and bacterial comm unity structure are not yet clear.A basal diet without chitosa n served as the control(CTL),and the treatment diets were supplemented with chitosan with different molecular weights:1000(1K),3000(3K),5000(5K),50000(5W)and 200000(20W)dry matter(DM).Six fermentation units per treatment were established.Gas chromatography was used to measure the co nee ntrations of metha ne,H2 and volatile fatty acids(VFAs).The bacterial 16S rRNA genes were sequeneed with an Illumina MiSeq platform and analysed to reveal the relative abunda nces of bacterial community taxa.The results showed that the propi onate proporti on was sign ifica ntly in creased by the addition of chitosan with different molecular weights(P<0.05),while methane production and the acetate proportion were significantly decreased(P<0.05).The relative mbundances of Rikenellaceae_RC9_gut_group and Prevotellaceae_UCG_003 were significa ntly in creased in the 3K chitosa n group compared with the CTL group,whereas the relative abunda nee of Ruminococcaceae_NK4A214_group was significantly decreased(P<0.05).Correlation analyses of the relative abundances of the bacterial genera showed that Prevotella was positively related to propionmte production(Pv0.05).In conclusion,3K chitosan could reduce methane production by replacing fibrolytic bacteria(Firmicutes and Fibrobacteres)with amylolytic bacteria(Bacteroidetes and Proteobacteria)in the bacterial community structure. | TONG Jin-jin ZHANG Hua WANG Jia LIU Yun MAO Sheng-yong XIONG Ben-hai JIANG Linshu | 2020 | Journal of Integrative Agriculture2020，19，6： | 0 |
| 19 | Novel chemical-and protein-mediated methods for glucosamine detection显示文摘We describe two novel approaches for the determination of glucosamine(GlcN).The first approach is based on the chemical derivatization of GlcN with the non-fluorophor 1,3-diphenyl-1,3-propanedione(DPPD),which results in a condensation product with interesting fluorescent properties.The obtained compound was isolated by silica-gel chromatography and its structure elucidated by NMR and mass spectrometry.The second approach is based on a previously undescribed sensitivity of the enzyme glucosamine-6-phosphate deaminase(GPDA)towards GlcN,which resulted in the precipitation of the enzyme.Using a rational enzyme engineering approach and both liquid-based and plate-based screening methods,mutational GPDA variants with significantly improved precipitation properties were identified and characterized.These novel glucosamine detection methods may be a useful addition to the repertoire of currently available glucosamine detection sensors. | Linshu Chen Pedro Laborda Zhipeng Cai Andrew Kevin Hagan Aimin Lu Josef Voglmeir Li Liu | 2022 | Food Materials Research2022，2，1： | 0 |
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